Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_C14
(507 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 169 7e-43
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 169 9e-43
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 169 9e-43
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 169 9e-43
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 89 2e-18
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 85 2e-17
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 85 3e-17
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 83 1e-16
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 83 1e-16
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 74 5e-14
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 69 2e-12
At4g18820.1 68417.m02778 expressed protein 29 2.4
At5g13030.1 68418.m01494 expressed protein contains Pfam profile... 28 4.2
At1g79060.1 68414.m09218 expressed protein 28 4.2
At5g18200.1 68418.m02136 expressed protein 27 7.3
At4g30360.1 68417.m04314 cyclic nucleotide-regulated ion channel... 27 7.3
At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl... 27 9.6
At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative simila... 27 9.6
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 169 bits (412), Expect = 7e-43
Identities = 79/128 (61%), Positives = 101/128 (78%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
IAV T +GLVVPV+R+ + M++ IE +N LA+KA G ++ +M GG+FT+SNGGV+G
Sbjct: 330 IAVGTSKGLVVPVIRDADKMNFADIEKTINGLAKKATEGTISIDEMAGGSFTVSNGGVYG 389
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIKGKVEIRPMMYLALSYDHRLIDGREAVLFLRK 475
SL+S PIIN PQS ILGMH+I QRP+ + G V RPMMY+AL+YDHRLIDGREAV FLR+
Sbjct: 390 SLISTPIINPPQSAILGMHSIVQRPMVVGGSVVPRPMMYVALTYDHRLIDGREAVYFLRR 449
Query: 476 VKAGVEDP 499
+K VEDP
Sbjct: 450 IKDVVEDP 457
>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 365
Score = 169 bits (411), Expect = 9e-43
Identities = 79/128 (61%), Positives = 100/128 (78%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
IAV T +GLVVPV+R + M++ IE +N+LA+KA G ++ +M GG+FT+SNGGV+G
Sbjct: 231 IAVGTSKGLVVPVIRGADKMNFAEIEKTINSLAKKANEGTISIDEMAGGSFTVSNGGVYG 290
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIKGKVEIRPMMYLALSYDHRLIDGREAVLFLRK 475
SL+S PIIN PQS ILGMH+I RP+ + G V RPMMY+AL+YDHRLIDGREAV FLR+
Sbjct: 291 SLISTPIINPPQSAILGMHSIVSRPMVVGGSVVPRPMMYVALTYDHRLIDGREAVYFLRR 350
Query: 476 VKAGVEDP 499
VK VEDP
Sbjct: 351 VKDVVEDP 358
>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 463
Score = 169 bits (411), Expect = 9e-43
Identities = 79/128 (61%), Positives = 100/128 (78%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
IAV T +GLVVPV+R + M++ IE +N+LA+KA G ++ +M GG+FT+SNGGV+G
Sbjct: 329 IAVGTSKGLVVPVIRGADKMNFAEIEKTINSLAKKANEGTISIDEMAGGSFTVSNGGVYG 388
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIKGKVEIRPMMYLALSYDHRLIDGREAVLFLRK 475
SL+S PIIN PQS ILGMH+I RP+ + G V RPMMY+AL+YDHRLIDGREAV FLR+
Sbjct: 389 SLISTPIINPPQSAILGMHSIVSRPMVVGGSVVPRPMMYVALTYDHRLIDGREAVYFLRR 448
Query: 476 VKAGVEDP 499
VK VEDP
Sbjct: 449 VKDVVEDP 456
>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 169 bits (411), Expect = 9e-43
Identities = 79/128 (61%), Positives = 100/128 (78%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
IAV T +GLVVPV+R + M++ IE +N+LA+KA G ++ +M GG+FT+SNGGV+G
Sbjct: 330 IAVGTSKGLVVPVIRGADKMNFAEIEKTINSLAKKANEGTISIDEMAGGSFTVSNGGVYG 389
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIKGKVEIRPMMYLALSYDHRLIDGREAVLFLRK 475
SL+S PIIN PQS ILGMH+I RP+ + G V RPMMY+AL+YDHRLIDGREAV FLR+
Sbjct: 390 SLISTPIINPPQSAILGMHSIVSRPMVVGGSVVPRPMMYVALTYDHRLIDGREAVYFLRR 449
Query: 476 VKAGVEDP 499
VK VEDP
Sbjct: 450 VKDVVEDP 457
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 88.6 bits (210), Expect = 2e-18
Identities = 48/132 (36%), Positives = 77/132 (58%), Gaps = 5/132 (3%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
IAVAT +GL+ P+++N + I L + LA+KAR+G L P + QGGTF+ISN G++
Sbjct: 500 IAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNLGMYP 559
Query: 296 SLLSMPIINMPQSCILGM---HAIFQRPVAIKG--KVEIRPMMYLALSYDHRLIDGREAV 460
IIN PQ+ IL + + + + + + G K + M + LS DHR+ DG+
Sbjct: 560 VDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDGQVGA 619
Query: 461 LFLRKVKAGVED 496
F+ ++++ ED
Sbjct: 620 SFMSELRSNFED 631
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 85.4 bits (202), Expect = 2e-17
Identities = 50/133 (37%), Positives = 73/133 (54%), Gaps = 3/133 (2%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISN-GGVF 292
+AV T GL VPV+++ + I + LA+KA+ +L P D +GGTFT+SN GG F
Sbjct: 404 VAVQTENGLYVPVVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNLGGPF 463
Query: 293 GSLLSMPIINMPQSCILGMHAIFQRPVAIKG--KVEIRPMMYLALSYDHRLIDGREAVLF 466
G +IN PQ+ IL + + +R V G + + M + LS DHR+IDG +
Sbjct: 464 GIKQFCAVINPPQAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAIGAEW 523
Query: 467 LRKVKAGVEDPNS 505
L+ K +E P S
Sbjct: 524 LKAFKGYIETPES 536
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 84.6 bits (200), Expect = 3e-17
Identities = 48/133 (36%), Positives = 73/133 (54%), Gaps = 3/133 (2%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISN-GGVF 292
+AV T GL VPV+++ + I + LA+KA+ +L P D +GGTFT+SN GG F
Sbjct: 404 VAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGTFTVSNLGGPF 463
Query: 293 GSLLSMPIINMPQSCILGMHAIFQRPVAIKGKVEIRPMMYL--ALSYDHRLIDGREAVLF 466
G ++N PQ+ IL + + +R V G + Y+ LS DHR++DG +
Sbjct: 464 GIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHRVVDGAIGAEW 523
Query: 467 LRKVKAGVEDPNS 505
L+ K +E+P S
Sbjct: 524 LKAFKGYIENPKS 536
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 82.6 bits (195), Expect = 1e-16
Identities = 45/129 (34%), Positives = 69/129 (53%), Gaps = 1/129 (0%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
+A+AT GLVVP ++NV+S+ I ++ L A L P D+ GGT T+SN G G
Sbjct: 347 VAMATEHGLVVPNIKNVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIG 406
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIK-GKVEIRPMMYLALSYDHRLIDGREAVLFLR 472
P++N+P+ I+ + I + P K G V +M + ++ DHR++DG F
Sbjct: 407 GKFGSPLLNLPEVAIIALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCC 466
Query: 473 KVKAGVEDP 499
+ K VE P
Sbjct: 467 QWKEYVEKP 475
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 82.6 bits (195), Expect = 1e-16
Identities = 45/129 (34%), Positives = 69/129 (53%), Gaps = 1/129 (0%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
+A+AT GLVVP ++NV+S+ I ++ L A L P D+ GGT T+SN G G
Sbjct: 347 VAMATEHGLVVPNIKNVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIG 406
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIK-GKVEIRPMMYLALSYDHRLIDGREAVLFLR 472
P++N+P+ I+ + I + P K G V +M + ++ DHR++DG F
Sbjct: 407 GKFGSPLLNLPEVAIIALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCC 466
Query: 473 KVKAGVEDP 499
+ K VE P
Sbjct: 467 QWKEYVEKP 475
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 74.1 bits (174), Expect = 5e-14
Identities = 43/129 (33%), Positives = 69/129 (53%), Gaps = 1/129 (0%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
+AVA GL+ PVL+N + +D + L +KAR L P + GTFT+SN G+FG
Sbjct: 332 VAVAIDGGLITPVLQNADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFG 391
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIK-GKVEIRPMMYLALSYDHRLIDGREAVLFLR 472
I+ I+ + A VA K G++ ++ M + ++ DHR+I G + FL+
Sbjct: 392 VDRFDAILPPGTGAIMAVGASQPSVVATKDGRIGMKNQMQVNVTADHRVIYGADLAQFLQ 451
Query: 473 KVKAGVEDP 499
+ + +EDP
Sbjct: 452 TLASIIEDP 460
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 68.9 bits (161), Expect = 2e-12
Identities = 42/131 (32%), Positives = 68/131 (51%), Gaps = 1/131 (0%)
Frame = +2
Query: 116 IAVATPRGLVVPVLRNVESMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFG 295
+AVA GL+ PVL++ + +D + L KAR+ L P + GTFT+SN G+FG
Sbjct: 347 VAVAINGGLITPVLQDADKLDLYLLSQKWKELVGKARSKQLQPHEYNSGTFTLSNLGMFG 406
Query: 296 SLLSMPIINMPQSCILGMHAIFQRPVAIK-GKVEIRPMMYLALSYDHRLIDGREAVLFLR 472
I+ Q I+ + A VA K G ++ M + ++ DHR++ G + FL+
Sbjct: 407 VDRFDAILPPGQGAIMAVGASKPTVVADKDGFFSVKNTMLVNVTADHRIVYGADLAAFLQ 466
Query: 473 KVKAGVEDPNS 505
+E+P+S
Sbjct: 467 TFAKIIENPDS 477
>At4g18820.1 68417.m02778 expressed protein
Length = 1111
Score = 28.7 bits (61), Expect = 2.4
Identities = 21/78 (26%), Positives = 39/78 (50%)
Frame = +2
Query: 197 AMNALAEKARTGNLTPADMQGGTFTISNGGVFGSLLSMPIINMPQSCILGMHAIFQRPVA 376
A+ +A ++ G+L A+M T+ + G +S+P++ S +L H I Q V
Sbjct: 629 ALKLIASRS-DGSLRDAEM-----TLEQLSLLGQRISVPLVQELVSIVLSHHHIKQLKVG 682
Query: 377 IKGKVEIRPMMYLALSYD 430
+ ++ ++ LALS D
Sbjct: 683 LVSDEKLVDLLDLALSAD 700
>At5g13030.1 68418.m01494 expressed protein contains Pfam profile
PF02696: Uncharacterized ACR, YdiU/UPF0061 family
Length = 633
Score = 27.9 bits (59), Expect = 4.2
Identities = 16/39 (41%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -3
Query: 130 CCHSYYFYFKVI-AFCSTGSSPFLASSSRTADNLAPPRS 17
CC S +FY + + F ST PF S R L+P RS
Sbjct: 6 CCPSSFFYLRPLHLFSSTAKVPFCPSLPRQF-RLSPSRS 43
>At1g79060.1 68414.m09218 expressed protein
Length = 396
Score = 27.9 bits (59), Expect = 4.2
Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 4/68 (5%)
Frame = +2
Query: 170 SMDYPRIELAMNALAEKARTGNLTPADMQGGTFTISNGGVFGSLLS----MPIINMPQSC 337
S+D PR+ + + + + +P+ GGT + G+ S S P++N+P
Sbjct: 289 SVDSPRLNSSGKIVFQNLERSSSSPSSFNGGTSGYRHRGMERSYSSNVRVTPVLNVPVCS 348
Query: 338 ILGMHAIF 361
I G +F
Sbjct: 349 IRGGSVVF 356
>At5g18200.1 68418.m02136 expressed protein
Length = 351
Score = 27.1 bits (57), Expect = 7.3
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = -1
Query: 351 CIPRMQDCGMFMIGIDNNEPNTPPLLIVNVPPCMSAGVK 235
CI R Q+C + + +++PN +I N+ P +S ++
Sbjct: 66 CIGREQECAPELFRVPDHDPNWKLRVIENLYPALSRNLE 104
>At4g30360.1 68417.m04314 cyclic nucleotide-regulated ion channel,
putative (CNGC17) similar to cyclic nucleotide and
calmodulin-regulated ion channel cngc5 GI:4581205 from
[Arabidopsis thaliana]
Length = 720
Score = 27.1 bits (57), Expect = 7.3
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 474 FLRNNTASRPSINLWSYESARYIIGLISTLPL 379
F R P W Y + +II LI+TLPL
Sbjct: 158 FGRGELVMDPKAIAWRYIKSDFIIDLIATLPL 189
>At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast
(FAD7) (FADD) identical to omega-3 fatty acid
desaturase, chloroplast precursor SP:P46310 [Arabidopsis
thaliana (Mouse-ear cress)]; identical to Pfam profile
PF00487: Fatty acid desaturase; identical to cDNA
plastid fatty acid desaturase GI:809491
Length = 446
Score = 26.6 bits (56), Expect = 9.6
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -1
Query: 492 STPALTFLRNNTASRPSINLWSYESA 415
+TP FL NN RPS++ SY+++
Sbjct: 19 TTPRSNFLSNNNKFRPSLSSSSYKTS 44
>At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative similar
to RNA helicase GI:3776027 from [Arabidopsis thaliana]
Length = 513
Score = 26.6 bits (56), Expect = 9.6
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +2
Query: 113 IIAVATPRGLVVPVLRNVESMDY-PRIELAMNALAEKARTG 232
I+ RG+ +P+L NV + D+ PR ++ ++ + AR G
Sbjct: 326 IVTDIAARGIDIPLLDNVINWDFPPRPKIFVHRVGRAARAG 366
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,262,211
Number of Sequences: 28952
Number of extensions: 235573
Number of successful extensions: 741
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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