Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_B21
(626 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 155 3e-38
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 154 5e-38
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 151 4e-37
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 150 9e-37
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 91 7e-19
At4g39060.1 68417.m05532 F-box family protein low similarity to ... 30 1.1
At4g01810.1 68417.m00238 protein transport protein-related relat... 29 3.3
At2g37660.1 68415.m04619 expressed protein 28 4.4
At1g66950.1 68414.m07612 ABC transporter family protein similar ... 28 4.4
At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containi... 28 5.8
At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 27 7.7
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 155 bits (375), Expect = 3e-38
Identities = 67/122 (54%), Positives = 88/122 (72%)
Frame = +3
Query: 189 AFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIG 368
A AG+++G+EIWRI+NF+PV V + GKF+ GDSYI+LKTT+ + +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 369 SETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFN 548
++SQDEAGA A++TV LD GG AVQ+RE GHE+ FL YF + +GG SGFN
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFN 130
Query: 549 HV 554
HV
Sbjct: 131 HV 132
Score = 41.1 bits (92), Expect = 6e-04
Identities = 32/153 (20%), Positives = 65/153 (42%), Gaps = 2/153 (1%)
Frame = +3
Query: 111 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 290
+++Q VN Q + + +K P G +++WRI E + +++ + KFY
Sbjct: 364 LQRQGVNVQ-GLVKTSSSSSKDEPKPYIDGTGN---LQVWRINCEEKILLEAAEQSKFYS 419
Query: 291 GDSYIILKT--TSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREA 464
GD YI+ + D+ +L + W G ++ +++ +A L + + Q R
Sbjct: 420 GDCYILQYSYPGEDREEHL---VGTWFGKQSVEEDRASAISLANKMVESMKFVPAQARIN 476
Query: 465 MGHESNHFLQYFTSALQYLDGGNPSGFNHVVTN 563
G E F S + + G + + ++ N
Sbjct: 477 EGKEPIQFFVIMQSFITFKGGVSDAFKKYIAEN 509
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 154 bits (373), Expect = 5e-38
Identities = 70/124 (56%), Positives = 85/124 (68%)
Frame = +3
Query: 186 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 365
PAF AG++AG+EIWRI+NF P + GKF+ GDSYI+LKTT+ K L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 366 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 545
G +TSQDEAG AA+ TV LD GG AVQ+RE GHE+ FL YF + +GG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGF 129
Query: 546 NHVV 557
HVV
Sbjct: 130 KHVV 133
Score = 43.2 bits (97), Expect = 1e-04
Identities = 26/113 (23%), Positives = 47/113 (41%)
Frame = +3
Query: 219 VEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGA 398
+++WR+ +Q+ D KFY GD Y+ + + I W G ++ ++E G+
Sbjct: 394 LQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKE-EVLIGTWFGKQSVEEERGS 452
Query: 399 AAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVV 557
A + + + Q R G E F S + + GG SG+ +
Sbjct: 453 AVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSGYKKYI 504
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 151 bits (366), Expect = 4e-37
Identities = 70/123 (56%), Positives = 85/123 (69%)
Frame = +3
Query: 186 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 365
PAF AG++ G EIWRI+NFE V V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 366 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 545
G +TSQDEAG AA+ TV LD GG AVQHRE GHES+ FL YF + L+GG SGF
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 546 NHV 554
V
Sbjct: 128 KTV 130
Score = 51.6 bits (118), Expect = 4e-07
Identities = 32/130 (24%), Positives = 53/130 (40%)
Frame = +3
Query: 156 KDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRN 335
K A + P + G+ +E+W + + +D GK Y GD Y++L T
Sbjct: 374 KSAPVNEDIPPLLESGGK---LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGER 430
Query: 336 NLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQ 515
+ + W G ++ ++ A L + + G VQ R G E F+ F +
Sbjct: 431 KDEYFLSCWFGKKSIPEDQDTAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALF-QPMV 489
Query: 516 YLDGGNPSGF 545
L GG SG+
Sbjct: 490 VLKGGLSSGY 499
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 150 bits (363), Expect = 9e-37
Identities = 68/120 (56%), Positives = 84/120 (70%)
Frame = +3
Query: 186 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 365
PAF G++ G EIWRI+NFEPV V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 366 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 545
G +TSQDEAG AA+ TV LD GG AVQ+RE GHES+ FL YF + L+GG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Score = 54.8 bits (126), Expect = 4e-08
Identities = 39/154 (25%), Positives = 63/154 (40%)
Frame = +3
Query: 111 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 290
++QQ V + LS K + P G+ +E+W I + GK Y
Sbjct: 364 LKQQGVG--LKGLS-KSTPVNEDIPPLLEGGGK---LEVWYIDANSKTVLSKDHVGKLYS 417
Query: 291 GDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMG 470
GD Y++L T + + W G ++Q++ A L + + G VQ R G
Sbjct: 418 GDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLASTMTNSLKGRPVQARIFEG 477
Query: 471 HESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGA 572
E F+ F + L GG SG+ + +T G+
Sbjct: 478 KEPPQFVALF-QHMVVLKGGLSSGYKNSMTEKGS 510
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 90.6 bits (215), Expect = 7e-19
Identities = 39/122 (31%), Positives = 70/122 (57%)
Frame = +3
Query: 162 ARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNL 341
+R + AF G ++G+EIW + N + +++ FGKF+ G++Y++L+T K +
Sbjct: 2 SRLSKDIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESP 61
Query: 342 SWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYL 521
+DIHYW+G + ++ ++ A+ + LD G VQ+RE G E+ FL YF + +
Sbjct: 62 QYDIHYWLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPV 121
Query: 522 DG 527
+G
Sbjct: 122 EG 123
Score = 35.9 bits (79), Expect = 0.022
Identities = 26/115 (22%), Positives = 48/115 (41%), Gaps = 1/115 (0%)
Frame = +3
Query: 186 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYII-LKTTSDKRNNLSWDIHYW 362
P + N + +++WR+ + + D K + GD Y++ K T +R ++ W
Sbjct: 382 PLYTNC--RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL--LYVW 437
Query: 363 IGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDG 527
IG E+ Q + A + G +V G+E + F F S + + G
Sbjct: 438 IGCESIQQDRADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVFKGG 492
>At4g39060.1 68417.m05532 F-box family protein low similarity to
SKP1 interacting partner 6 [Arabidopsis thaliana]
GI:10716957; contains Pfam profile PF00646: F-box domain
Length = 375
Score = 30.3 bits (65), Expect = 1.1
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -2
Query: 568 PGFVTTWLKPDGLPPSKYCSADVKYCRKWLLSWPIASRCCTAAPPNL 428
P + + W+KPD ++YC+ K R L+ P+ S T PPNL
Sbjct: 64 PIWFSLWIKPDDQTLNQYCNIQEKSTRNLLV--PVPS-SYTPFPPNL 107
>At4g01810.1 68417.m00238 protein transport protein-related related
to Sec23 protein [Homo sapiens] gi|1296664|emb|CAA65774
Length = 880
Score = 28.7 bits (61), Expect = 3.3
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +3
Query: 249 PVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSW--DIHYWIGSETSQDEAGAAAIL 410
P + ++ G F + +Y L SDK L D+ W+G+E S DE +AA+L
Sbjct: 733 PRCLMHQEGGTFEELPAYD-LSMQSDKAVILDHGTDVFIWLGAELSADEVKSAAVL 787
>At2g37660.1 68415.m04619 expressed protein
Length = 325
Score = 28.3 bits (60), Expect = 4.4
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +3
Query: 360 WIGSETSQDEAGAAAILTVGLDDKFGGAAVQH-REAMGHESNHFLQYFTSALQYL-DGGN 533
WIG + D A AA + + L GG + H ++G+ + L + A QYL D G
Sbjct: 182 WIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSIGNA--NILVWKRKAEQYLADSGI 239
Query: 534 P 536
P
Sbjct: 240 P 240
>At1g66950.1 68414.m07612 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]
Length = 1454
Score = 28.3 bits (60), Expect = 4.4
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +3
Query: 438 GAAVQHREAMGHESNHFLQYFTS---ALQYLDGGNPSGFNHVVTNPGAEKRM 584
G V + ++GH S ++YF + + DG NP+ + VT P E +M
Sbjct: 1081 GGQVIYAGSLGHHSQKLVEYFEAVEGVPKINDGYNPATWMLDVTTPSMESQM 1132
>At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containing
protein low similiarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile: PF01535 PPR
repeat
Length = 409
Score = 27.9 bits (59), Expect = 5.8
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = -3
Query: 321 MSSLELYRNLLCRTSQSLSIGLRLVQNSESAKF 223
M++ L+ +L CR + + +G ++N+ES++F
Sbjct: 1 MATRSLFHSLRCRLTNNGVLGSNFIRNAESSRF 33
>At3g29810.1 68416.m03794 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL2 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 441
Score = 27.5 bits (58), Expect = 7.7
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -2
Query: 592 PXNIRFSAPGFVTTWLKPDGLPPSKYCSADVKYCRKWLLSWPI 464
P NI APG T + PS++ SAD + + LL+W I
Sbjct: 168 PRNITLKAPGPGYTCGPAKLVKPSRFISADKRRKTQSLLTWNI 210
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,810,000
Number of Sequences: 28952
Number of extensions: 321028
Number of successful extensions: 874
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 873
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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