Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_B11
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 66 3e-11
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 64 6e-11
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 63 1e-10
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 62 3e-10
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 61 6e-10
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 60 1e-09
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 57 1e-08
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 55 4e-08
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 54 6e-08
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 52 3e-07
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 49 2e-06
At4g01220.2 68417.m00161 expressed protein 29 3.6
At4g01220.1 68417.m00160 expressed protein 29 3.6
At3g10120.1 68416.m01214 expressed protein 28 4.8
At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein... 28 4.8
At1g30650.1 68414.m03748 WRKY family transcription factor contai... 28 6.3
At3g47810.3 68416.m05210 calcineurin-like phosphoesterase family... 27 8.3
At3g47810.1 68416.m05209 calcineurin-like phosphoesterase family... 27 8.3
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 65.7 bits (153), Expect = 3e-11
Identities = 25/51 (49%), Positives = 38/51 (74%)
Frame = +3
Query: 501 TLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
++ E + H+ DDCWVVI +V+D+S+++DEHPGG D++L AG DA+ F
Sbjct: 9 SMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDF 59
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 64.5 bits (150), Expect = 6e-11
Identities = 25/52 (48%), Positives = 38/52 (73%)
Frame = +3
Query: 498 ITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
I+ +V+ H +DCW++I+ +V+DISTF+DEHPGG +++L G DAS F
Sbjct: 5 ISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDF 56
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 63.3 bits (147), Expect = 1e-10
Identities = 25/54 (46%), Positives = 37/54 (68%)
Frame = +3
Query: 492 RTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
+ TL EVS H + DCW+VI +V+D++ FLD+HPGG +++L G DA+ F
Sbjct: 6 KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDF 59
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 62.1 bits (144), Expect = 3e-10
Identities = 23/62 (37%), Positives = 46/62 (74%)
Frame = +3
Query: 468 PGAEDAKDRTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDAST 647
P + + ++ + EV++H+ +DCW++I D+V+DI+++++EHPGG D +L++AG D++
Sbjct: 39 PVEDVVRPKSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTD 97
Query: 648 AF 653
F
Sbjct: 98 GF 99
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 61.3 bits (142), Expect = 6e-10
Identities = 24/54 (44%), Positives = 37/54 (68%)
Frame = +3
Query: 492 RTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
+ TL EVS H+ DCW+VI +V++++ FL++HPGG D++L G DA+ F
Sbjct: 6 KIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDF 59
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 60.1 bits (139), Expect = 1e-09
Identities = 21/54 (38%), Positives = 37/54 (68%)
Frame = +3
Query: 492 RTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
+ ++ EVS H+ DCW++I +V+D++ F+D+HPGG +++L G DA+ F
Sbjct: 6 KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDF 59
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 56.8 bits (131), Expect = 1e-08
Identities = 20/51 (39%), Positives = 35/51 (68%)
Frame = +3
Query: 501 TLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
++ EV H++ D CW++++ ++D + FL +HPGG+D +L AGTD + F
Sbjct: 546 SMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEF 596
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 55.2 bits (127), Expect = 4e-08
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 1/76 (1%)
Frame = +3
Query: 429 VESEKNKWTDQIIPGA-EDAKDRTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGG 605
+ SE N + + + + ++ EV H+T D W++++ ++D + FL +HPGG
Sbjct: 524 ISSESNNTLKKSVSSPFMNTASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGG 583
Query: 606 ADIMLEYAGTDASTAF 653
D +L AGTD + F
Sbjct: 584 TDSILINAGTDCTEEF 599
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 54.4 bits (125), Expect = 6e-08
Identities = 22/60 (36%), Positives = 35/60 (58%)
Frame = +3
Query: 474 AEDAKDRTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
A+ K R +T ++ H+ P D W+ I +V+D+S ++ HPGG +L AG D + AF
Sbjct: 2 ADQTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 52.0 bits (119), Expect = 3e-07
Identities = 21/58 (36%), Positives = 35/58 (60%)
Frame = +3
Query: 480 DAKDRTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
++ R I + EV H T D W V+ RV++IS +++ HPGG D++++ G D + F
Sbjct: 123 ESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLF 180
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 49.2 bits (112), Expect = 2e-06
Identities = 19/60 (31%), Positives = 35/60 (58%)
Frame = +3
Query: 474 AEDAKDRTITLVEVSLHDTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTAF 653
AE+ + + IT ++ H+ D W+ I +V+++S ++ HPGG ++L G D + AF
Sbjct: 2 AEETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At4g01220.2 68417.m00161 expressed protein
Length = 299
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +3
Query: 327 EIKLSTAIPVNQDNSELDLMALTMAALRFVNPWTVESEKNKWTDQIIPGAED 482
+ L A+ N + + A++ L F+N W + + K DQ++ AED
Sbjct: 80 DYSLPQAVKFVAKNGTVIVCAVSYPYLPFLNNWLISVSRQKHQDQVLVIAED 131
>At4g01220.1 68417.m00160 expressed protein
Length = 360
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +3
Query: 327 EIKLSTAIPVNQDNSELDLMALTMAALRFVNPWTVESEKNKWTDQIIPGAED 482
+ L A+ N + + A++ L F+N W + + K DQ++ AED
Sbjct: 80 DYSLPQAVKFVAKNGTVIVCAVSYPYLPFLNNWLISVSRQKHQDQVLVIAED 131
>At3g10120.1 68416.m01214 expressed protein
Length = 173
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/54 (29%), Positives = 30/54 (55%)
Frame = +3
Query: 360 QDNSELDLMALTMAALRFVNPWTVESEKNKWTDQIIPGAEDAKDRTITLVEVSL 521
Q+ + + M TM +RF NP VE E+ + D++ ++ K++T +V V +
Sbjct: 70 QETNSIKHMKKTMKKVRFANP-EVEKEEQE-EDRLTDCCDNTKEKTNGVVRVKM 121
>At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 100
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +3
Query: 525 DTPDDCWVVIYDRVFDISTFLDEHPGGADIMLEYAGTDASTA 650
D +V I RVFD++T + G D + +AG DAS A
Sbjct: 15 DESKPIYVAIKGRVFDVTTGKSFYGSGGDYSM-FAGKDASRA 55
>At1g30650.1 68414.m03748 WRKY family transcription factor contains
Pfam profile: PF03106 WRKY DNA -binding domain
Length = 430
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/53 (28%), Positives = 26/53 (49%)
Frame = -1
Query: 632 PGVLQHNICSTRMFIEECGDIKDSIVNNDPAIVRGVMQADFYKSYCTILSILR 474
P + + N + + GD ++I NN+ +V V + D KS C+I +R
Sbjct: 68 PLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCSIFPRIR 120
>At3g47810.3 68416.m05210 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 190
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 632 PGVLQHNICSTRMFIEECGDIKDSIVNNDPAIVRGVMQAD 513
PG +QH IC+ + I+E D +I D IVRG D
Sbjct: 29 PGKIQHIICTGNLCIKEIHDYLKTIC-PDLHIVRGEFDED 67
>At3g47810.1 68416.m05209 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 190
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 632 PGVLQHNICSTRMFIEECGDIKDSIVNNDPAIVRGVMQAD 513
PG +QH IC+ + I+E D +I D IVRG D
Sbjct: 29 PGKIQHIICTGNLCIKEIHDYLKTIC-PDLHIVRGEFDED 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,806,164
Number of Sequences: 28952
Number of extensions: 241306
Number of successful extensions: 713
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 702
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 712
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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