Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0005_A05
(562 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g24230.1 68418.m02851 expressed protein 32 0.23
At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family prote... 31 0.53
At1g06590.1 68414.m00698 expressed protein 30 0.92
At5g67040.1 68418.m08452 hypothetical protein contains Pfam prof... 30 1.2
At1g10040.1 68414.m01132 expressed protein non-consensus GC dono... 30 1.2
At5g57840.1 68418.m07233 transferase family protein similar to a... 29 2.1
At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase (X... 29 2.8
At5g24220.1 68418.m02850 lipase class 3-related 28 4.9
At4g10550.1 68417.m01727 subtilase family protein contains simil... 28 4.9
At5g59400.2 68418.m07444 expressed protein predicted protein, Ar... 27 6.5
At5g59400.1 68418.m07443 expressed protein predicted protein, Ar... 27 6.5
At4g10955.1 68417.m01782 lipase class 3 family protein contains ... 27 6.5
At1g26120.1 68414.m03188 esterase-related contains similaity to ... 27 6.5
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 27 8.6
At2g34500.1 68415.m04237 cytochrome P450 family protein similar ... 27 8.6
At1g68960.1 68414.m07893 hypothetical protein contains Pfam prof... 27 8.6
At1g44130.1 68414.m05097 nucellin protein, putative similar to n... 27 8.6
At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase f... 27 8.6
>At5g24230.1 68418.m02851 expressed protein
Length = 369
Score = 32.3 bits (70), Expect = 0.23
Identities = 29/87 (33%), Positives = 45/87 (51%)
Frame = +3
Query: 12 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLTGLQVGR 191
LH +T RF+ + + +A+AK G + A+ L GHSLG+ ++ AGK T +G +
Sbjct: 149 LHDST--RFV-HAIQVIQSAVAKTGNA--AVWLAGHSLGAAVALLAGKIMTR-SGFPLES 202
Query: 192 ISGLDPAGPCFSNIPAELRLKHTDAKY 272
P FS+IP E +K K+
Sbjct: 203 YL----FNPPFSSIPIEKLVKSEKLKH 225
>At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family protein
similar to protein phosphatase methylesterase-1 [Homo
sapiens] GI:5533003; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 350
Score = 31.1 bits (67), Expect = 0.53
Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 3/46 (6%)
Frame = +3
Query: 57 EVLAAMAK-GGMSPDAIHLIGHSLGSHIS--GFAGKTFTTLTGLQV 185
+VLA + + G SP AI L+GHS+G ++ A KT +L GL V
Sbjct: 134 DVLAVIKELYGDSPPAIVLVGHSMGGSVAVQVAANKTLPSLAGLVV 179
>At1g06590.1 68414.m00698 expressed protein
Length = 916
Score = 30.3 bits (65), Expect = 0.92
Identities = 17/56 (30%), Positives = 27/56 (48%)
Frame = +3
Query: 309 LKEPCGHADYYPNSGSQQPNCLFQTCSHSRAVLLYGESIGNVNAFPAVRCKDWESF 476
L++P G P+SGS++ + FQ C H V + G + A +R WE +
Sbjct: 542 LQKPWGPPVISPDSGSRKSSTFFQLCDH--LVSIPGSVSQLIGASYLLRATSWELY 595
>At5g67040.1 68418.m08452 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 165
Score = 29.9 bits (64), Expect = 1.2
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 36 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH 134
F+G L A G+ P++I+ IGH LGS+
Sbjct: 104 FLGASEAFCLTASMYPGLKPNSIYYIGHGLGSY 136
>At1g10040.1 68414.m01132 expressed protein non-consensus GC donor
splice site at exon boundary 21576
Length = 412
Score = 29.9 bits (64), Expect = 1.2
Identities = 19/48 (39%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = +3
Query: 15 HST-TY--VRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFA 149
HST T+ V +GE+L E + ++ K S I +GHSLG I+ +A
Sbjct: 120 HSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYA 167
>At5g57840.1 68418.m07233 transferase family protein similar to
anthranilate N-hydroxycinnamoyl/benzoyltransferase from
Dianthus caryophyllus [gi:2239091]
Length = 443
Score = 29.1 bits (62), Expect = 2.1
Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 2/80 (2%)
Frame = -3
Query: 488 LSDSK--GLPVLAPDSWECVHVADRLAVEQHGATVAARLEKAVRLLGSAVRVVVGVTTGF 315
++DSK VL +W C A+ E+H + ++ RL + +G T
Sbjct: 247 IADSKFSTYEVLVAHTWRCASFANEDLSEEHSTRLHIIIDGRPRLQPKLPQGYIGNTLFH 306
Query: 314 L*PVNSAIGVYHVDVFSVCV 255
PV S +G +H + FS V
Sbjct: 307 ARPV-SPLGAFHRESFSETV 325
>At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase
(XSP1) identical to subtilisin-type serine endopeptidase
XSP1 GI:6708179 from [Arabidopsis thaliana]
Length = 749
Score = 28.7 bits (61), Expect = 2.8
Identities = 23/94 (24%), Positives = 41/94 (43%), Gaps = 9/94 (9%)
Frame = +3
Query: 57 EVLAAMAKGGMSPDAIHLIGHSLGSHISGFAG-----KTFT----TLTGLQVGRISGLDP 209
+V+ + G++PD+ + H LG + + G K FT + G + + G P
Sbjct: 138 DVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVP 197
Query: 210 AGPCFSNIPAELRLKHTDAKYVDVIHTDGGVYGL 311
AG S I + HT + V+ + +YG+
Sbjct: 198 AGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGI 231
>At5g24220.1 68418.m02850 lipase class 3-related
Length = 376
Score = 27.9 bits (59), Expect = 4.9
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +3
Query: 90 SPDAIHLIGHSLGSHISGFAGKTFTT 167
S AI L GHSLG+ + AGKT T+
Sbjct: 171 SEAAIWLAGHSLGAALVLLAGKTMTS 196
>At4g10550.1 68417.m01727 subtilase family protein contains
similarity to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana]
Length = 778
Score = 27.9 bits (59), Expect = 4.9
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +3
Query: 39 IGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH-ISGFAGKTFTTLTGLQVGRISGLDPAG 215
+G+ +G +A + G + A ++ + + +S A T TT + +SG A
Sbjct: 500 VGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQGFIMLSGTSMAA 559
Query: 216 PCFSNIPAELRLKHTD 263
P S + A L+ H D
Sbjct: 560 PAISGVAALLKALHRD 575
>At5g59400.2 68418.m07444 expressed protein predicted protein,
Arabidopsis thaliana
Length = 301
Score = 27.5 bits (58), Expect = 6.5
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +1
Query: 7 CTSIRPRTFGS*ERSSVKSSRLWQRA 84
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At5g59400.1 68418.m07443 expressed protein predicted protein,
Arabidopsis thaliana
Length = 299
Score = 27.5 bits (58), Expect = 6.5
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +1
Query: 7 CTSIRPRTFGS*ERSSVKSSRLWQRA 84
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At4g10955.1 68417.m01782 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 350
Score = 27.5 bits (58), Expect = 6.5
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +3
Query: 12 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKT 158
LH+TT + + ++A++ GG S + L GHSLG+ ++ GKT
Sbjct: 136 LHTTTRFEIAIQAVRNIVASV--GGSS---VWLAGHSLGASMALLTGKT 179
>At1g26120.1 68414.m03188 esterase-related contains similaity to
esterase 6 GI:606998 from [Drosophila simulans] and
esterase GI:12584120 from [Sphingomonas elodea]
Length = 476
Score = 27.5 bits (58), Expect = 6.5
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +3
Query: 72 MAKGGMSPDAIHLIGHSLGSHIS 140
+A+ G PD I+L+G S G+HI+
Sbjct: 270 IAEYGGDPDRIYLMGQSAGAHIA 292
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 27.1 bits (57), Expect = 8.6
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = +3
Query: 36 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT--GLQVGRISGLDP 209
F+ +K+ V A A G++ A+ + G S A K F + GL + LDP
Sbjct: 328 FVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISESE-ATKNFYLIDKDGLVTTERTKLDP 386
Query: 210 AGPCFSNIPAELR 248
F+ PAE+R
Sbjct: 387 GAVLFAKNPAEIR 399
>At2g34500.1 68415.m04237 cytochrome P450 family protein similar to
Cytochrome P450 61 (C-22 sterol desaturase) (SP:P54781)
{Saccharomyces cerevisiae}
Length = 495
Score = 27.1 bits (57), Expect = 8.6
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +3
Query: 93 PDAIHLIGHSLGSHISG 143
PDA HLIGH G + G
Sbjct: 101 PDAFHLIGHPFGKKLFG 117
>At1g68960.1 68414.m07893 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 376
Score = 27.1 bits (57), Expect = 8.6
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +3
Query: 36 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT 173
F+GE L+A G++P++++ GH G I A T L+
Sbjct: 313 FLGENESFCLSAKDFPGLNPNSVYFAGHGSGYGIWDLASCTIRYLS 358
>At1g44130.1 68414.m05097 nucellin protein, putative similar to
nucellin GI:2290202 from [Hordeum vulgare]
Length = 405
Score = 27.1 bits (57), Expect = 8.6
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = +1
Query: 172 PDFKSAGYRDWIRRDPASVIFPLS 243
P KS+ ++ +I+ P+SV+FPLS
Sbjct: 17 PLSKSSIFKTFIKSSPSSVVFPLS 40
>At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase
family protein contains Pfam profile PF01553:
Acyltransferase
Length = 585
Score = 27.1 bits (57), Expect = 8.6
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = -2
Query: 159 TSYQRNLICESRGCALSDESHRDSY 85
T YQ L+ ++R C++S + +RD++
Sbjct: 82 TKYQELLLDKNRACSVSSDHYRDTF 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,587,159
Number of Sequences: 28952
Number of extensions: 262165
Number of successful extensions: 732
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 715
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 731
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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