BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_P20 (201 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) ide... 27 1.3 At5g07910.1 68418.m00914 leucine-rich repeat family protein cont... 27 1.7 At1g04050.1 68414.m00392 SET domain-containing protein / suppres... 27 2.3 At1g27430.1 68414.m03343 GYF domain-containing protein contains ... 26 3.0 At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family pr... 26 4.0 At1g36310.1 68414.m04513 expressed protein 25 5.2 At3g53030.1 68416.m05845 protein kinase family protein contains ... 25 6.9 At3g49220.1 68416.m05379 pectinesterase family protein contains ... 25 6.9 At1g67420.1 68414.m07674 24 kDa vacuolar protein, putative simil... 25 6.9 At4g17340.1 68417.m02601 major intrinsic family protein / MIP fa... 25 9.2 At3g49450.1 68416.m05405 F-box family protein contains F-box dom... 25 9.2 At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138... 25 9.2 >At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) identical to sterol-C5-desaturase GB:AAD12944 GI:4234768 from [Arabidopsis thaliana] Length = 281 Score = 27.5 bits (58), Expect = 1.3 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +1 Query: 58 ASPWRNILQFLPTINNTLLLRASYKRLGSIPEFSW 162 A PW +L PT++ +++ R K SI EF W Sbjct: 94 AMPWYTLL---PTVSESMIERGWTKCFASIGEFGW 125 >At5g07910.1 68418.m00914 leucine-rich repeat family protein contains leucine rich repeat (LRR) domains, Pfam:PF00560 Length = 262 Score = 27.1 bits (57), Expect = 1.7 Identities = 15/32 (46%), Positives = 20/32 (62%), Gaps = 4/32 (12%) Frame = +1 Query: 70 RNILQFLP----TINNTLLLRASYKRLGSIPE 153 RN+L +LP ++ N LLL S RL S+PE Sbjct: 123 RNMLIYLPDTIGSLRNLLLLNVSNNRLKSLPE 154 >At1g04050.1 68414.m00392 SET domain-containing protein / suppressor of variegation related 1 (SUVR1) identical to suppressor of variegation related 1 [Arabidopsis thaliana] GI:15004614; contains Pfam profiles PF00856: SET domain, PF05033: Pre-SET motif; identical to cDNA trithorax 3 (ATX3) partial cds GI:15217142 Length = 630 Score = 26.6 bits (56), Expect = 2.3 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -1 Query: 183 NQFNRRVPREFRYGPKPFV 127 N+ N +VP FRY P FV Sbjct: 319 NEINEKVPSRFRYMPHSFV 337 >At1g27430.1 68414.m03343 GYF domain-containing protein contains Pfam profile: PF02213 GYF domain Length = 1531 Score = 26.2 bits (55), Expect = 3.0 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = -2 Query: 161 QENSGMDPSRL*DARSNNVLLIVGRNCNIFRHGD 60 + NS +DP+ DA S N + N F HGD Sbjct: 719 RRNSQIDPNISLDALSGNRMSQFEHESNFFNHGD 752 >At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family protein similar to CHY1 [gi:8572760]; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 409 Score = 25.8 bits (54), Expect = 4.0 Identities = 12/34 (35%), Positives = 15/34 (44%) Frame = +2 Query: 71 EIYYNFYQQLTTRYYFERLTNGLGPYLNSLGTRL 172 E+ F+ YY RL LG YL G +L Sbjct: 174 EVQIGFHPDAGASYYLSRLPGYLGEYLALTGQKL 207 >At1g36310.1 68414.m04513 expressed protein Length = 404 Score = 25.4 bits (53), Expect = 5.2 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = +2 Query: 62 RRGEIYYNFYQQLTTRYYFERLTNGLG 142 R+G + YN Y + + ERL +G+G Sbjct: 352 RKGAVVYNRYYHVFSEGELERLASGVG 378 >At3g53030.1 68416.m05845 protein kinase family protein contains eukaryotic protein kinase domain, INTERPRO:IPR000719 Length = 529 Score = 25.0 bits (52), Expect = 6.9 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = +2 Query: 41 RYGNLKHRRGEIYYNFYQQLTTRYYF-ERLTNGLGPYLNSL 160 R+G+L+H R ++ + LT +Y F E+ N L +L S+ Sbjct: 406 RHGDLRHIRRLRFWPMNKVLTEKYEFSEQDANDLSDFLVSI 446 >At3g49220.1 68416.m05379 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 598 Score = 25.0 bits (52), Expect = 6.9 Identities = 17/45 (37%), Positives = 23/45 (51%) Frame = +2 Query: 47 GNLKHRRGEIYYNFYQQLTTRYYFERLTNGLGPYLNSLGTRLLNW 181 G H RG + +N L T YY E L +G G + LG R ++W Sbjct: 518 GGHVHTRGWLEWNTTFALDTLYYGEYLNSGPG---SGLGQR-VSW 558 >At1g67420.1 68414.m07674 24 kDa vacuolar protein, putative similar to 24 kDa vacuolar protein VP24 [Ipomoea batatas] gi|5821406|dbj|BAA83809 Length = 873 Score = 25.0 bits (52), Expect = 6.9 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +2 Query: 80 YNFYQQLTTRYYFERLTNGLGPYLNSLGTRLLNWL 184 + FY T+ Y+F L G ++ S+ + LL W+ Sbjct: 497 FGFYAFATSAYFFAGLNGGFMTFVISI-SMLLGWI 530 >At4g17340.1 68417.m02601 major intrinsic family protein / MIP family protein contains Pfam profile: MIP PF00230 Length = 250 Score = 24.6 bits (51), Expect = 9.2 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 21 LSGGVLRDMVTLSIAVAKYITIST 92 +SGG L VTL +AV IT+ T Sbjct: 77 ISGGHLNPAVTLGLAVGGNITVIT 100 >At3g49450.1 68416.m05405 F-box family protein contains F-box domain Pfam:PF00646 Length = 397 Score = 24.6 bits (51), Expect = 9.2 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -2 Query: 185 VTSLIGEYQENSGMDPSRL 129 ++S +GEY ENSG P+ L Sbjct: 18 ISSSVGEYGENSGTLPTDL 36 >At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'growth regulator protein -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 499 Score = 24.6 bits (51), Expect = 9.2 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = -3 Query: 157 RIQVWTQAVCKTLEVITC 104 R+QVW VC ++ V TC Sbjct: 27 RVQVWFVRVCSSILVWTC 44 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,634,354 Number of Sequences: 28952 Number of extensions: 75338 Number of successful extensions: 210 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 210 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 12,070,560 effective HSP length: 46 effective length of database: 10,738,768 effective search space used: 214775360 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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