Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_P16
(580 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f... 95 4e-20
At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ... 33 0.18
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 32 0.24
At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC1... 32 0.32
At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding ... 30 1.3
At4g18320.1 68417.m02717 hypothetical protein 29 1.7
At2g30100.1 68415.m03663 ubiquitin family protein low similarity... 29 2.2
At4g05100.1 68417.m00758 myb family transcription factor (MYB74)... 29 3.0
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 29 3.0
At1g02990.2 68414.m00269 expressed protein similar to mature-par... 29 3.0
At1g02990.1 68414.m00270 expressed protein similar to mature-par... 29 3.0
At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase f... 28 3.9
At2g03240.1 68415.m00277 EXS family protein / ERD1/XPR1/SYG1 fam... 28 3.9
At4g25350.1 68417.m03648 EXS family protein / ERD1/XPR1/SYG1 fam... 28 5.2
At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to... 28 5.2
At3g18080.1 68416.m02299 glycosyl hydrolase family 1 protein con... 28 5.2
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam... 28 5.2
At4g28650.1 68417.m04095 leucine-rich repeat transmembrane prote... 27 6.8
At3g44530.1 68416.m04786 transducin family protein / WD-40 repea... 27 6.8
At3g16555.1 68416.m02115 F-box family protein contains F-box dom... 27 6.8
>At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to synaptic glycoprotein SC2 spliced
variant from Homo sapiens [EMBL:AF038958], SC2 from
Rattus sp. [gi:256994]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 310
Score = 94.7 bits (225), Expect = 4e-20
Identities = 60/169 (35%), Positives = 88/169 (52%), Gaps = 12/169 (7%)
Frame = +3
Query: 108 MEIEILSVAGSKPL-GKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEA------KG 266
M++ ++S +G + L + L + +T+ +++E H K YP RQ + L K
Sbjct: 1 MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAK-KFYPSRQRLTLPVTPGSKDKP 59
Query: 267 KTLNDEDTLKSLNIQNGYKLYV--KDLGPQISWKNVFLAEYAGPLFVYLWVYQRP---WI 431
LN + +LK N L V KDLG Q+S++ +F EY GPL +Y Y P ++
Sbjct: 60 VVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFL 119
Query: 432 LYGEQTSTPGHVATVAAVCWSAHYAKRLFETQFIHRFSHGTMPLTNLFK 578
YGE V T A W HY KR+ ET F+HRFSH T P+ N+F+
Sbjct: 120 GYGEDCVIHP-VQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFR 167
>At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam
profile: PF00240 ubiquitin family
Length = 158
Score = 32.7 bits (71), Expect = 0.18
Identities = 26/76 (34%), Positives = 41/76 (53%)
Frame = +3
Query: 105 IMEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDE 284
+M I I ++ G + + +++ TI+N+K KI K + D+Q +L KGK L D
Sbjct: 85 VMFIFIKTLTGRRIV--LEVENCDTIENIKAKIQD--KQGIPVDQQ--RLIFKGKQLEDG 138
Query: 285 DTLKSLNIQNGYKLYV 332
TL NIQN L++
Sbjct: 139 LTLADYNIQNDSILHL 154
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 32.3 bits (70), Expect = 0.24
Identities = 26/75 (34%), Positives = 38/75 (50%)
Frame = +3
Query: 108 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 287
M+I I ++ G + +++ TI NVKEKI VK + PD+Q L G+ L D
Sbjct: 552 MQIFIKTLTGKTII--LEVESSDTIANVKEKI--QVKEGIKPDQQ--MLIFFGQQLEDGV 605
Query: 288 TLKSLNIQNGYKLYV 332
TL +I LY+
Sbjct: 606 TLGDYDIHKKSTLYL 620
Score = 27.5 bits (58), Expect = 6.8
Identities = 19/59 (32%), Positives = 26/59 (44%)
Frame = +3
Query: 156 IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYV 332
+++ TI NVK K+ + + R L GK L TL NIQ G LY+
Sbjct: 257 LEVESSDTIDNVKAKLQDKERIPMDLHR----LIFAGKPLEGGRTLAHYNIQKGSTLYL 311
>At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC13
[Arabidopsis thaliana] gi|21105767|gb|AAM34782; contains
Pfam domains, PF00439: Bromodomain and PF00240:
Ubiquitin family
Length = 1919
Score = 31.9 bits (69), Expect = 0.32
Identities = 18/68 (26%), Positives = 38/68 (55%)
Frame = +3
Query: 108 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 287
M+I + S+ G K+ + E ++ +++ K + + + +A+K+ KGK L+DE
Sbjct: 661 MKIIVKSLGGKG--SKLHVGIEESVSSLRAKASRKLD---FKETEAVKMFYKGKELDDEK 715
Query: 288 TLKSLNIQ 311
+L + N+Q
Sbjct: 716 SLAAQNVQ 723
>At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding
bromodomain-containing protein low similarity to
SP|P51123 Transcription initiation factor TFIID 230 kDa
subunit {Drosophila melanogaster}; contains Pfam
profiles: PF00439 bromodomain, PF00240: Ubiquitin family
Length = 1700
Score = 29.9 bits (64), Expect = 1.3
Identities = 17/68 (25%), Positives = 36/68 (52%)
Frame = +3
Query: 108 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 287
M+I++ ++ G L + + E ++ ++K K + + + +A+K+ GK L DE
Sbjct: 500 MKIKLSNLGGKGSL--LTVGREESVSSLKAKASRKLD---FKETEAVKMFYMGKELEDEK 554
Query: 288 TLKSLNIQ 311
+L N+Q
Sbjct: 555 SLAEQNVQ 562
>At4g18320.1 68417.m02717 hypothetical protein
Length = 320
Score = 29.5 bits (63), Expect = 1.7
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 342 GPQISWKNVFLAEYAGPLFVYLWVYQRPWILYGEQTSTPGH 464
G W+N FL+ G +V +RPW+LY E+ S H
Sbjct: 44 GVNTHWRNTFLSFREGG-----FVEERPWLLYRERGSRVAH 79
>At2g30100.1 68415.m03663 ubiquitin family protein low similarity to
SP|Q9UQ13 Leucine-rich repeat protein SHOC-2
(Ras-binding protein Sur-8) {Homo sapiens}; contains
Pfam profiles PF00240: Ubiquitin family, PF01535: PPR
repeat, PF00560: Leucine Rich Repeat
Length = 897
Score = 29.1 bits (62), Expect = 2.2
Identities = 18/57 (31%), Positives = 33/57 (57%)
Frame = +3
Query: 156 IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKL 326
+ ++ + T+K++K ++ T++ P Q KL KGK L + TLK ++ +G KL
Sbjct: 549 LSVSPDCTVKDLKSQLQPI--TNVLPRGQ--KLIFKGKVLVETSTLKQSDVGSGAKL 601
>At4g05100.1 68417.m00758 myb family transcription factor (MYB74)
contains Pfam profile: PF00249 myb-like DNA-binding
domain; identical to cDNA putative transcription
factor (MYB74) mRNA, partial cds GI:3941505
Length = 324
Score = 28.7 bits (61), Expect = 3.0
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 476 HSCYMTRSRSLFSIKNPRPLVNPKVYE*WPGIFSQK 369
H +M SR + S N +PLVNP++ + +FS +
Sbjct: 155 HQQHMNMSRLMMSDGNHQPLVNPEILKLATSLFSNQ 190
>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor, Homo
sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1793
Score = 28.7 bits (61), Expect = 3.0
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -1
Query: 325 SLYPFCMFKDFRVSSSFKVL-PLASNLIACRSGYNEVFT 212
+L C + + K L PL +NLI+C G NEV T
Sbjct: 1737 TLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQT 1775
>At1g02990.2 68414.m00269 expressed protein similar to
mature-parasite-infected erythrocyte surface antigen
(GI:160409) {Plasmodium falciparum}
Length = 1238
Score = 28.7 bits (61), Expect = 3.0
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +3
Query: 168 EESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYVKDLGP 347
E+ +I+N+K K K+ YP I+ ++K K+ +E + S + G V+DLG
Sbjct: 807 EDCSIENLKPK-----KSGRYPGENCIEGDSKQKSREEESSAPSKDNNWGLVNNVQDLGT 861
Query: 348 QISWK 362
+ K
Sbjct: 862 AVKVK 866
>At1g02990.1 68414.m00270 expressed protein similar to
mature-parasite-infected erythrocyte surface antigen
(GI:160409) {Plasmodium falciparum}
Length = 1069
Score = 28.7 bits (61), Expect = 3.0
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +3
Query: 168 EESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYVKDLGP 347
E+ +I+N+K K K+ YP I+ ++K K+ +E + S + G V+DLG
Sbjct: 807 EDCSIENLKPK-----KSGRYPGENCIEGDSKQKSREEESSAPSKDNNWGLVNNVQDLGT 861
Query: 348 QISWK 362
+ K
Sbjct: 862 AVKVK 866
>At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to steroid 5alpha-reductase - Rattus
norvegicus, PIR:A34239 [SP|24008]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 268
Score = 28.3 bits (60), Expect = 3.9
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 498 HYAKRLFETQFIHRFSHG 551
H+ KR+FE FIH++S G
Sbjct: 104 HFFKRVFEVLFIHKYSGG 121
>At2g03240.1 68415.m00277 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 823
Score = 28.3 bits (60), Expect = 3.9
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +1
Query: 352 YLGRTYFWLNMPGHYSYTFGFTKG 423
Y G Y+W +YS+ FGF G
Sbjct: 476 YAGNIYYWRRYRVNYSFIFGFKHG 499
>At4g25350.1 68417.m03648 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 745
Score = 27.9 bits (59), Expect = 5.2
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +1
Query: 352 YLGRTYFWLNMPGHYSYTFGFTKG 423
Y YFW +YS+ FGF +G
Sbjct: 398 YAANIYFWRRYRVNYSFIFGFKQG 421
>At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 360
Score = 27.9 bits (59), Expect = 5.2
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +3
Query: 351 ISWKNVFLAEYAGPLFVYLWVYQRPWILY 437
+SW+ +F YAG + L VY + W +Y
Sbjct: 244 VSWEELFTILYAGIIASGLVVYLQTWCIY 272
>At3g18080.1 68416.m02299 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to beta-glucosidase BGQ60 precursor GB:A57512
[Hordeum vulgare]; similar to beta-mannosidase enzyme
(GI:17226270) [Lycopersicon esculentum]
Length = 512
Score = 27.9 bits (59), Expect = 5.2
Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 4/39 (10%)
Frame = +3
Query: 333 KDLGPQISWKNVF----LAEYAGPLFVYLWVYQRPWILY 437
KDLG Q W F L + GP W+Y PW +Y
Sbjct: 362 KDLGYQQDWNVEFGFAKLGKPIGPRAYSSWLYNVPWGMY 400
>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 756
Score = 27.9 bits (59), Expect = 5.2
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +1
Query: 352 YLGRTYFWLNMPGHYSYTFGFTKG 423
Y YFW +YS+ FGF +G
Sbjct: 411 YAANIYFWRQYRVNYSFIFGFKQG 434
>At4g28650.1 68417.m04095 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase 5,
Arabidopsis thaliana, PIR1:S27756
Length = 1013
Score = 27.5 bits (58), Expect = 6.8
Identities = 15/34 (44%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Frame = +3
Query: 117 EILSVAG-SKPLGKIQLNEESTIKNVKEKIHSSV 215
+++S+ G +KP K NEE+T +++ EK HSSV
Sbjct: 971 DVISMLGEAKPRRKSNSNEENTSRSLAEK-HSSV 1003
>At3g44530.1 68416.m04786 transducin family protein / WD-40 repeat
family protein contains 6 (4 significant) WD-40 repeats
(PF0400); nuclear protein HIRA, mouse, PIR:S68141
Length = 1051
Score = 27.5 bits (58), Expect = 6.8
Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = +3
Query: 105 IMEIEILSVAGSKPLGK-IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLND 281
++E AGSK +Q N+ +T +V + + + S DR E+ G+TLN
Sbjct: 397 LLETASTKQAGSKRAASDVQQNQVTTKPSVSVESTAKRRKSQVDDRNKAA-ESTGQTLNK 455
Query: 282 EDTLKSLNIQNGYKLY 329
TL ++ K+Y
Sbjct: 456 ASTLNRVSSPVNQKVY 471
>At3g16555.1 68416.m02115 F-box family protein contains F-box domain
Pfam:PF00646
Length = 349
Score = 27.5 bits (58), Expect = 6.8
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +2
Query: 56 LLIQTCNRFQIFWIIYNNGDRNFKRCWFQTSRKNSVK*RIYYKECKRKD 202
L I C+ I WI Y N N + +R V + Y EC+R++
Sbjct: 95 LTITACDGRWIKWIEYENKGFNVCGVGYDNTRPEKVYKILEYLECRREE 143
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,908,837
Number of Sequences: 28952
Number of extensions: 243452
Number of successful extensions: 624
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 623
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1131744440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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