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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_P07
         (326 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g13680.1 68416.m01727 F-box family protein contains F-box dom...    29   0.97 
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl...    28   1.3  
At3g50380.1 68416.m05511 expressed protein                             26   6.8  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    26   6.8  
At5g18710.1 68418.m02220 hypothetical protein predicted proteins...    25   9.0  
At1g60980.1 68414.m06864 gibberellin 20-oxidase, putative simila...    25   9.0  

>At3g13680.1 68416.m01727 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 395

 Score = 28.7 bits (61), Expect = 0.97
 Identities = 15/38 (39%), Positives = 21/38 (55%)
 Frame = +3

Query: 12  ITSLLAIHSTVQRTRRASKSLICSLRMSSYTTKASTFL 125
           +TSL AI ST Q+    SKS IC  + ++   K   F+
Sbjct: 21  LTSLRAIRSTCQKWNSLSKSQICGRKATAAENKFLGFM 58


>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
           identical to ubiquitin-protein ligase 1 [Arabidopsis
           thaliana] GI:7108521; E3, HECT-domain protein family;
           similar to GI:7108521, GB:AAF36454 from [Arabidopsis
           thaliana]
          Length = 3891

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = -2

Query: 169 NIIGFRKVRGNVEFVRKVLAFVVYEDILKEHIRL 68
           N++ F    G  EFV +++  V YED + E IR+
Sbjct: 534 NVVSF--FNGEPEFVNELVTLVSYEDTVPEKIRI 565


>At3g50380.1 68416.m05511 expressed protein
          Length = 3071

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = -2

Query: 94  DILKEHIRLFEARRVRWTVEWIAKRLV 14
           DI+ E+  LF    + W +EW  K LV
Sbjct: 661 DIVFEYSSLFSLALLIWQIEWAQKLLV 687


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
           identical to ubiquitin-protein ligase 2 [Arabidopsis
           thaliana] GI:7108523; E3, HECT-domain protein family;
           similar to ubiquitin-protein ligase 2 GI:7108523 from
           [Arabidopsis thaliana]
          Length = 3658

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = -2

Query: 169 NIIGFRKVRGNVEFVRKVLAFVVYEDILKEHIRL 68
           N++ F    G  EFV +++  V YED +   IR+
Sbjct: 285 NVVSF--FNGEPEFVNELVTLVSYEDTVPAKIRI 316


>At5g18710.1 68418.m02220 hypothetical protein predicted proteins -
           Arabidopsis thaliana
          Length = 563

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 11/42 (26%), Positives = 24/42 (57%)
 Frame = -2

Query: 142 GNVEFVRKVLAFVVYEDILKEHIRLFEARRVRWTVEWIAKRL 17
           G ++ +  V AF   + ++K H+   +A  ++  ++WI KR+
Sbjct: 365 GKIQKITFVQAFE-QDPVVKLHVGRLKATVIKGVIQWIDKRM 405


>At1g60980.1 68414.m06864 gibberellin 20-oxidase, putative similar
           to gibberellin 20-oxidase GB:CAA58295 from [Arabidopsis
           thaliana]
          Length = 376

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 10/36 (27%), Positives = 17/36 (47%)
 Frame = +2

Query: 65  KEPNMFFKDVFVYHEGEHFPYKFNVPPYFSKTNDVP 172
           K P   F    + H+ +H P +F  P +   + +VP
Sbjct: 18  KNPLRIFDSTVLNHQPDHIPQEFVWPDHEKPSKNVP 53


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,202,572
Number of Sequences: 28952
Number of extensions: 91583
Number of successful extensions: 220
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 370553816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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