BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_O18 (227 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g80070.1 68414.m09373 splicing factor, putative strong simila... 159 2e-40 At4g38780.1 68417.m05491 splicing factor, putative strong simila... 158 4e-40 At5g23200.1 68418.m02713 expressed protein 27 1.3 At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family pro... 27 2.2 At5g08270.1 68418.m00974 expressed protein 26 2.9 At5g15920.1 68418.m01862 structural maintenance of chromosomes (... 26 3.9 At3g06240.1 68416.m00717 F-box family protein contains F-box dom... 25 5.1 At2g14800.1 68415.m01677 hypothetical protein 25 5.1 At3g46420.1 68416.m05032 leucine-rich repeat family protein / pr... 25 6.8 At1g67140.1 68414.m07638 expressed protein 25 6.8 At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT... 25 8.9 At4g19530.1 68417.m02873 disease resistance protein (TIR-NBS-LRR... 25 8.9 At1g28020.1 68414.m03431 pentatricopeptide (PPR) repeat-containi... 25 8.9 >At1g80070.1 68414.m09373 splicing factor, putative strong similarity to splicing factor Prp8 [Homo sapiens] GI:3661610; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family Length = 2382 Score = 159 bits (387), Expect = 2e-40 Identities = 73/75 (97%), Positives = 75/75 (100%) Frame = +2 Query: 2 YRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEV 181 YRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK+IHTSVWAGQKRLGQLAKWKTAEEV Sbjct: 1833 YRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEV 1892 Query: 182 AALIRSLPVEEQPKQ 226 AAL+RSLPVEEQPKQ Sbjct: 1893 AALVRSLPVEEQPKQ 1907 >At4g38780.1 68417.m05491 splicing factor, putative strong similarity to splicing factor Prp8 [Homo sapiens] GI:3661610; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family Length = 2332 Score = 158 bits (384), Expect = 4e-40 Identities = 73/75 (97%), Positives = 74/75 (98%) Frame = +2 Query: 2 YRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEV 181 YRVTIHKTFEGNLTTKPING IFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEV Sbjct: 1785 YRVTIHKTFEGNLTTKPINGVIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEV 1844 Query: 182 AALIRSLPVEEQPKQ 226 AAL+RSLPVEEQPKQ Sbjct: 1845 AALVRSLPVEEQPKQ 1859 >At5g23200.1 68418.m02713 expressed protein Length = 399 Score = 27.5 bits (58), Expect = 1.3 Identities = 16/53 (30%), Positives = 24/53 (45%) Frame = +2 Query: 68 FIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ 226 F NPR Q + ++ RLG+ A+ AEE+ + + EQ KQ Sbjct: 21 FAKNPRQLQFEADVNKLFMYTSYNRLGREAEETDAEEIIEMAGKATLSEQQKQ 73 >At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family protein similar to ubiquitin-conjugating enzyme GB:3319990 from [Mus musculus]; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 1163 Score = 26.6 bits (56), Expect = 2.2 Identities = 15/46 (32%), Positives = 26/46 (56%) Frame = +2 Query: 83 RTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQP 220 + G++ L +I T W G+KR L K T ++ I++L + E+P Sbjct: 686 KCGKVCLSLIST--WTGKKREKWLPKESTMLQLLVSIQALILNEKP 729 >At5g08270.1 68418.m00974 expressed protein Length = 386 Score = 26.2 bits (55), Expect = 2.9 Identities = 17/53 (32%), Positives = 23/53 (43%) Frame = +2 Query: 68 FIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ 226 F NPR Q I ++ RLG+ A+ AEE+ + EQ KQ Sbjct: 26 FAKNPRQLQFEADINKLFMFTSYNRLGRDAEEADAEEIIEMAGKATFSEQQKQ 78 >At5g15920.1 68418.m01862 structural maintenance of chromosomes (SMC) family protein (MSS2) similar to SMC-related protein MSS2 [Arabidopsis thaliana] GI:9965743; contains Pfam profiles PF02483: SMC family C-terminal domain, PF02463: RecF/RecN/SMC N terminal domain Length = 1053 Score = 25.8 bits (54), Expect = 3.9 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +3 Query: 45 RSLSTVLYSYSTPELDNCSSR 107 RS+ST+LY S +L NC R Sbjct: 945 RSVSTILYLVSLQDLTNCPFR 965 >At3g06240.1 68416.m00717 F-box family protein contains F-box domain Pfam:PF00646 Length = 427 Score = 25.4 bits (53), Expect = 5.1 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = +2 Query: 8 VTIHKTFEGNLTTKPINGAIFIFNPRTG 91 V I + G + P GA+F++NP TG Sbjct: 163 VEIVGSSNGLVCISPGEGAVFLYNPTTG 190 >At2g14800.1 68415.m01677 hypothetical protein Length = 580 Score = 25.4 bits (53), Expect = 5.1 Identities = 13/30 (43%), Positives = 22/30 (73%) Frame = +3 Query: 33 VI*LRSLSTVLYSYSTPELDNCSSRSFIRV 122 +I L+ L+TVL+SY P+L + S+S++ V Sbjct: 524 IINLQQLATVLHSYEKPKLGD-FSQSYLLV 552 >At3g46420.1 68416.m05032 leucine-rich repeat family protein / protein kinase family protein contains leucine rich repeat (LRR) domains, INTERPRO:IPR001611; contains serine/threonine protein kinases active-site signature, Prosite:PS00108 Length = 838 Score = 25.0 bits (52), Expect = 6.8 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = -1 Query: 227 SVWVALQLAVSELEQPLLQRSSISRAVLDASD 132 SVW AL+LA+S +R ++S+ V+D + Sbjct: 771 SVWRALELAMSCANPTSEKRPNMSQVVIDLKE 802 >At1g67140.1 68414.m07638 expressed protein Length = 2158 Score = 25.0 bits (52), Expect = 6.8 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +1 Query: 49 AYQRCYIHIQPQNWTIVPQDHS 114 AY+R ++H+ P + I+PQ S Sbjct: 622 AYKRIFLHLYPHVFRIMPQKPS 643 >At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT3) identical to potassium channel [Arabidopsis thaliana] gi|1100898|gb|AAA97865; Note: also identical to AKT3 [Arabidopsis thaliana] gi|1172218|gb|AAA96153, which is a truncated version of AKT2, PMID:10852932; member of the 1 pore, 6 transmembrane (1P/6TM- Shaker-type) K+ channel family, PMID:11500563; identical to cDNA inward-rectifying K+ channel (AKT3) GI:1172219 Length = 802 Score = 24.6 bits (51), Expect = 8.9 Identities = 8/18 (44%), Positives = 9/18 (50%) Frame = +1 Query: 70 HIQPQNWTIVPQDHSYEC 123 HI+ W I P D Y C Sbjct: 60 HIRSSGWIISPMDSRYRC 77 >At4g19530.1 68417.m02873 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1167 Score = 24.6 bits (51), Expect = 8.9 Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 2/71 (2%) Frame = +2 Query: 20 KTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRL--GQLAKWKTAEEVAALI 193 K EG L T PI + P T + + ++ Q+ GQ+ KWK A +L+ Sbjct: 92 KELEGKLVTIPI---FYNVEPATVRYQKEAFGAALTKTQENDSDGQMKKWKEALTYVSLL 148 Query: 194 RSLPVEEQPKQ 226 P + K+ Sbjct: 149 VGFPFNSKSKE 159 >At1g28020.1 68414.m03431 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 612 Score = 24.6 bits (51), Expect = 8.9 Identities = 17/59 (28%), Positives = 25/59 (42%) Frame = +2 Query: 47 KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPK 223 KP + I N R + F K + S W G+K++ L A + L +EE K Sbjct: 399 KPSDLKCLIKNLRDSKQFSKALQVSEWMGEKQVCNLYLEDYAARLYLTENVLGLEEAEK 457 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,199,825 Number of Sequences: 28952 Number of extensions: 90249 Number of successful extensions: 253 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 250 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 253 length of database: 12,070,560 effective HSP length: 55 effective length of database: 10,478,200 effective search space used: 209564000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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