Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_O05
(550 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40942.1 68418.m04973 ribonucleoside-diphosphate reductase sm... 31 0.51
At5g05970.1 68418.m00661 transducin family protein / WD-40 repea... 28 0.66
At1g77270.1 68414.m08999 expressed protein 29 1.5
At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type f... 29 2.7
At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type f... 29 2.7
At5g26920.1 68418.m03210 calmodulin-binding protein similar to c... 28 3.6
At3g14850.2 68416.m01876 expressed protein 28 3.6
At3g14850.1 68416.m01877 expressed protein 28 3.6
At5g44120.3 68418.m05400 12S seed storage protein (CRA1) nearly ... 27 6.2
At5g58080.1 68418.m07268 two-component responsive regulator fami... 27 8.3
At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit... 27 8.3
At3g51560.1 68416.m05646 disease resistance protein (TIR-NBS-LRR... 27 8.3
At3g48500.1 68416.m05294 expressed protein 27 8.3
At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1) i... 27 8.3
>At5g40942.1 68418.m04973 ribonucleoside-diphosphate reductase small
chain, putative / ribonucleotide reductase, putative
similar to ribonucleotide reductase R2 [Nicotiana
tabacum] GI:1044912; contains Pfam profile PF00268:
Ribonucleotide reductase, small chain
Length = 231
Score = 31.1 bits (67), Expect = 0.51
Identities = 14/46 (30%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = -2
Query: 525 GFRLVFHNLHS-VVARTRVFDAQARVRLCGAIQQARVAVSDAVATA 391
GF++ N+HS ++ RT+ + + + +C A++ R V DA++ A
Sbjct: 101 GFQIAIENIHSEILLRTKFDEDRLKAIVCDAVEIEREFVCDALSCA 146
>At5g05970.1 68418.m00661 transducin family protein / WD-40 repeat
family protein contains similarity to regulatory protein
Nedd1; contains Pfam PF00400: WD domain, G-beta repeat
(6 copies, 2 weak)|19804256|gb|AV785466.1|AV785466
Length = 781
Score = 27.9 bits (59), Expect(2) = 0.66
Identities = 22/80 (27%), Positives = 34/80 (42%)
Frame = +1
Query: 229 PVCGTDGNTYPNKCSLECSRPLAPSLEMKHRGECQEVKVADIQPCICTREIKQVCGSDGV 408
PV G DG +C S S + G Q VK+ D+Q +C +++K G
Sbjct: 81 PVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIWDLQRKLCIKKLK---GHTST 137
Query: 409 TYGNPCLLNCATQSNPSLSI 468
G + NC + S+S+
Sbjct: 138 ITG--VMYNCKDEHLASVSV 155
Score = 21.4 bits (43), Expect(2) = 0.66
Identities = 6/21 (28%), Positives = 12/21 (57%)
Frame = +1
Query: 184 TCEEADPCVCTFIYAPVCGTD 246
+ + DPCV ++ +P C +
Sbjct: 27 SADSGDPCVLSYTPSPGCAVN 47
>At1g77270.1 68414.m08999 expressed protein
Length = 682
Score = 29.5 bits (63), Expect = 1.5
Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = +3
Query: 3 RYISDRRCANIRRNSAAVVMRVRKKLKTGL-RIRRQDLPQPVPLVLREGQDAQRFENRE 176
RY+ D + N R + + + R K+LKT + R + + P+V + +D QR +NRE
Sbjct: 391 RYVRDSQEKNTRNDESQTIFRASKRLKTEIGRADHNSISKTNPVV--KTKDIQR-KNRE 446
>At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type
family protein contains Pfam domain PF00050: Kazal-type
serine protease inhibitor domain
Length = 144
Score = 28.7 bits (61), Expect = 2.7
Identities = 18/49 (36%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = +1
Query: 52 PSSCA--CARNLRPVCGSDGKTYHNQCLLYCERDKTHSDLKIVKEGTCE 192
P+SC C R PVCG D TY C D +++VK+G C+
Sbjct: 68 PASCPVQCFRP-DPVCGEDSVTYWCGC-----ADALCHGVRVVKQGACD 110
Score = 27.5 bits (58), Expect = 6.2
Identities = 21/60 (35%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = +1
Query: 313 KHRGE-CQEVKVADIQPCICTREIKQVCGSDGVTYGNPCLLNCATQSNPSLSIEHPGPCD 489
K+RGE C+ + P C R VCG D VTY C CA + + G CD
Sbjct: 56 KNRGEFCEGIAKPASCPVQCFRP-DPVCGEDSVTYW--C--GCADALCHGVRVVKQGACD 110
>At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type
family protein contains Pfam domain PF00050: Kazal-type
serine protease inhibitor domain
Length = 117
Score = 28.7 bits (61), Expect = 2.7
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 85 PVCGSDGKTYHNQCLLYCERDKTHSDLKIVKEGTCE 192
PVCG+DG TY C D ++VK+G C+
Sbjct: 53 PVCGTDGVTYWCGC-----PDAACHGARVVKKGACD 83
>At5g26920.1 68418.m03210 calmodulin-binding protein similar to
calmodulin-binding protein TCB60 GI:1698548 from
[Nicotiana tabacum]
Length = 492
Score = 28.3 bits (60), Expect = 3.6
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -1
Query: 517 VGFPQPSLCCRKDPGVRCSSSGS 449
VG PQ SL C +DPG + SGS
Sbjct: 301 VGHPQRSLQCPQDPGFVVTCSGS 323
>At3g14850.2 68416.m01876 expressed protein
Length = 321
Score = 28.3 bits (60), Expect = 3.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 403 GVTYGNPCLLNCATQSNPSLSIEHPGPCDNRVKVVE 510
GV +G P +C Q P L ++PG V V++
Sbjct: 223 GVLWGEPAAKSCVGQKEPLLGTKYPGGLPAEVGVLK 258
>At3g14850.1 68416.m01877 expressed protein
Length = 253
Score = 28.3 bits (60), Expect = 3.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 403 GVTYGNPCLLNCATQSNPSLSIEHPGPCDNRVKVVE 510
GV +G P +C Q P L ++PG V V++
Sbjct: 155 GVLWGEPAAKSCVGQKEPLLGTKYPGGLPAEVGVLK 190
>At5g44120.3 68418.m05400 12S seed storage protein (CRA1) nearly
identical to SP|P15455 [Plant Mol Biol 11:805-820
(1988)]; contains Pfam profile PF00190 Cupin and Prosite
11-S plant seed storage proteins signature PS00305
Length = 472
Score = 27.5 bits (58), Expect = 6.2
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = +1
Query: 223 YAPVCGTDGNTYPNKCSLECSRPLAPSLEMKHRGECQEVKVAD 351
YA G G +PN+C L+ L PS +K E ++V D
Sbjct: 21 YAAQQGQQGQQFPNECQLDQLNALEPSHVLK--SEAGRIEVWD 61
>At5g58080.1 68418.m07268 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver domain
Length = 581
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1
Query: 433 NCATQSNPSLSIEHPGPCDNRV 498
NC +NP +IE+P P DN V
Sbjct: 477 NCTMLNNPFGNIEYPLPADNMV 498
>At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At2) / UFO-binding protein (UIP2) E3
ubiquitin ligase; skp1b; identical to UIP2 GI:3719211
from [Arabidopsis thaliana]; contains Pfam profiles
PF01466: Skp1 family, dimerisation domain and
PF03931:Skp1 family, tetramerisation domain; identical
to cDNA UFO binding protein UIP2 mRNA, partial cds
GI:3719210
Length = 171
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 50 CRRHARAQET*DRSADQTARLTTTSAS 130
C+RH A E + +AD A TTT+ +
Sbjct: 60 CKRHVEAAEKSETTADAAAATTTTTVA 86
>At3g51560.1 68416.m05646 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1253
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -3
Query: 404 PSLPQTCLISRVQIHGCISATLTSW 330
P+LPQ+ + + +HGC+S SW
Sbjct: 983 PALPQS--LKLLNVHGCVSLESVSW 1005
>At3g48500.1 68416.m05294 expressed protein
Length = 668
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 217 FIYAPVCGTDGNTYPNKCSLECSRPLAP 300
F + P+ DG+T P++ +C RP P
Sbjct: 354 FDFPPIFHRDGDTNPDEIRRDCGRPPEP 381
>At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1)
identical to GDP-mannose pyrophosphorylase from
Arabidopsis thaliana [GI:3598958]; updated per Conklin
PL et al, PNAS 1999, 96(7):4198-203
Length = 361
Score = 27.1 bits (57), Expect = 8.3
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = -3
Query: 374 RVQIHGCISATLTSWHS 324
R++ H CIS+++ WHS
Sbjct: 297 RIKKHACISSSIIGWHS 313
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,512,263
Number of Sequences: 28952
Number of extensions: 273458
Number of successful extensions: 885
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 851
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 884
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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