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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_O02
         (567 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g61160.2 68416.m06845 shaggy-related protein kinase beta / AS...    30   0.94 
At3g61160.1 68416.m06844 shaggy-related protein kinase beta / AS...    30   0.94 
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain...    29   1.6  
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain...    29   2.2  
At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identic...    28   5.0  
At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM ...    27   6.6  

>At3g61160.2 68416.m06845 shaggy-related protein kinase beta /
           ASK-beta (ASK2) identical to shaggy-related protein
           kinase beta SP:O23145 GI:2569931 from [Arabidopsis
           thaliana]
          Length = 438

 Score = 30.3 bits (65), Expect = 0.94
 Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = +2

Query: 446 IDVXSDIATDAVXKIF-LGPKYNDYGFPITLEENWHKFY 559
           +++   + T A  +I  + P+YND+ FP    + WHK +
Sbjct: 320 VEIIKILGTPAREEIKNMNPRYNDFKFPQIKAQPWHKIF 358


>At3g61160.1 68416.m06844 shaggy-related protein kinase beta /
           ASK-beta (ASK2) identical to shaggy-related protein
           kinase beta SP:O23145 GI:2569931 from [Arabidopsis
           thaliana]
          Length = 431

 Score = 30.3 bits (65), Expect = 0.94
 Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = +2

Query: 446 IDVXSDIATDAVXKIF-LGPKYNDYGFPITLEENWHKFY 559
           +++   + T A  +I  + P+YND+ FP    + WHK +
Sbjct: 313 VEIIKILGTPAREEIKNMNPRYNDFKFPQIKAQPWHKIF 351


>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to  Chain B, Crystal Structure Of N-Terminal Domain Of
           Drosophila Ap180 (GP:13399617) [Drosophila
           melanogaster]; supporting cDNA
           gi|20465326|gb|AY096427.1|
          Length = 601

 Score = 29.5 bits (63), Expect = 1.6
 Identities = 35/132 (26%), Positives = 59/132 (44%), Gaps = 14/132 (10%)
 Frame = +2

Query: 17  WQENADLYEEEVTKDYQ-RSYEIVARHV---LGAAPKPFD---KHTFMPSALDFYQTALR 175
           W     L+ EE  + Y+   Y+I A  +    GA+ K  D     T+    L   +   +
Sbjct: 139 WIRTYALFLEERLECYRVLKYDIEAERLPKGSGASSKNVDFNASQTYRTRMLSDEELLEQ 198

Query: 176 DPAFYQLYNRIVGYI---NAFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQ 334
            PA  QL  R++G     +A+ +YL  Y      K  F +   IND ++  +  FF+ S+
Sbjct: 199 LPALQQLLYRLIGCQPEGSAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSR 258

Query: 335 FDATNSVFLTKK 370
            DA  ++ + K+
Sbjct: 259 HDAVKALNIYKR 270


>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
           domain-containing protein / clathrin assembly
           protein-related low similarity to clathrin assembly
           protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
           profile PF01417: ENTH domain
          Length = 591

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 35/127 (27%), Positives = 56/127 (44%), Gaps = 9/127 (7%)
 Frame = +2

Query: 17  WQENADLYEEEVTKDYQ-RSYEIVARHVLGAAPKPFDKH-TFMPSALDFYQTALRDPAFY 190
           W     L+ EE  + Y+   Y+I A  +  A+      H T M S  D  +     PA  
Sbjct: 139 WVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRMLSGEDLLEQL---PALQ 195

Query: 191 QLYNRIVGYIN---AFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQFDATN 349
           QL  R++G      A+ +YL  Y      K  F +   IND ++  +  FF+ S+ DA  
Sbjct: 196 QLLYRLIGCQPEGAAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSRHDAVK 255

Query: 350 SVFLTKK 370
           ++ + K+
Sbjct: 256 ALNIYKR 262


>At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identical
           to SP|Q9ZWM9 DNA-binding protein RAV1 {Arabidopsis
           thaliana}, RAV1 GI:3868857 from [Arabidopsis thaliana]
          Length = 344

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 21/75 (28%), Positives = 35/75 (46%)
 Frame = +2

Query: 143 SALDFYQTALRDPAFYQLYNRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEKLVTFF 322
           SA   ++ A+      +L   ++   +A KH+  P P   +   GV +N   V   V  F
Sbjct: 183 SAEALFEKAVTPSDVGKLNRLVIPKHHAEKHF--PLPSSNVSVKGVLLNFEDVNGKVWRF 240

Query: 323 DYSQFDATNSVFLTK 367
            YS ++++ S  LTK
Sbjct: 241 RYSYWNSSQSYVLTK 255


>At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM
           TIGR01640 : F-box protein interaction domain;
          Length = 364

 Score = 27.5 bits (58), Expect = 6.6
 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
 Frame = -3

Query: 421 ESWLTNLEVVWVTSLNLFFGQEYTVSG--IKLAIVKECD*FLNDNI-IDF 281
           ESW  NL ++    ++ +F Q Y V G  ++ + V E     N+ + IDF
Sbjct: 39  ESWFVNLNLLRTNRISGYFIQHYIVKGHELRTSFVHERSDLQNNGVSIDF 88


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,619,069
Number of Sequences: 28952
Number of extensions: 226274
Number of successful extensions: 640
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 640
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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