Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_N20
         (548 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g06599.1 68417.m01024 ubiquitin family protein contains Pfam ...   113   6e-26
At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative...    43   1e-04
At5g38470.1 68418.m04650 DNA repair protein RAD23, putative simi...    39   0.002
At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative...    36   0.013
At2g30100.1 68415.m03663 ubiquitin family protein low similarity...    36   0.018
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu...    34   0.054
At1g53950.1 68414.m06145 ubiquitin family protein contains INTER...    33   0.13 
At1g64470.1 68414.m07309 ubiquitin family protein contains INTER...    29   2.7  
At5g66130.1 68418.m08331 cell cycle checkpoint protein-related w...    27   6.2  

>At4g06599.1 68417.m01024 ubiquitin family protein contains Pfam
           domian PF00240: Ubiquitin family
          Length = 340

 Score =  113 bits (273), Expect = 6e-26
 Identities = 49/75 (65%), Positives = 60/75 (80%)
 Frame = +1

Query: 322 KINKRVRDYQINLLNQPRPGKKLLVLDIDYTLFDHRSVAETGYELMRPFLHEFLTSAYVD 501
           K+ +R+  Y+INL    R GKKLLVLDIDYTLFDHRS AE   +LMRP+LHEFLT+AY +
Sbjct: 135 KLRRRIDQYKINLRTPCRQGKKLLVLDIDYTLFDHRSTAENPLQLMRPYLHEFLTAAYAE 194

Query: 502 YDLVIWSATGMTWIE 546
           YD++IWSAT M W+E
Sbjct: 195 YDIMIWSATSMKWVE 209



 Score = 58.8 bits (136), Expect = 2e-09
 Identities = 33/73 (45%), Positives = 46/73 (63%)
 Frame = +2

Query: 2   NGKEYDIPEFSPSDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPN 181
           NGKEY +      DSVA LK  I   T V P+RQKLL  K   K++ D+  LS ++ KP+
Sbjct: 31  NGKEYTV-RICADDSVAELKRRICLLTTVLPKRQKLLYPKIGNKLSDDSLLLSSISFKPS 89

Query: 182 LKIMMMGSLEEAI 220
           LK+ M+G++E+ I
Sbjct: 90  LKMTMIGTVEDDI 102


>At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative
           (UBP7) similar to GI:11993467
          Length = 532

 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 24/78 (30%), Positives = 41/78 (52%)
 Frame = +2

Query: 8   KEYDIPEFSPSDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPNLK 187
           K ++  E   S    + K  + + +GV PERQK++    +G +  D+   S L LK   K
Sbjct: 66  KVFESIEIDTSQPPFVFKAQLYDLSGVPPERQKIM---VKGGLLKDDADWSTLGLKNGQK 122

Query: 188 IMMMGSLEEAIQGAQTKP 241
           +MMMG+ +E ++  +  P
Sbjct: 123 LMMMGTADEIVKAPEKGP 140


>At5g38470.1 68418.m04650 DNA repair protein RAD23, putative similar
           to DNA repair by nucleotide excision (NER) RAD23
           protein, isoform I GI:1914683 from [Daucus carota]
          Length = 378

 Score = 39.1 bits (87), Expect = 0.002
 Identities = 29/79 (36%), Positives = 41/79 (51%), Gaps = 1/79 (1%)
 Frame = +2

Query: 2   NGKEYDIPEFSPSDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPN 181
           +G  ++I E  P+D V+ +K AIE   G      K + I  QGKV  D  TL + N+  N
Sbjct: 9   SGSNFEI-EVKPADKVSDVKTAIETVKGAEYPAAKQMLI-HQGKVLKDETTLEENNVVEN 66

Query: 182 LKIMMMGSLEEAI-QGAQT 235
             I++M S  +A   GA T
Sbjct: 67  SFIVIMLSKTKASPSGAST 85


>At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative
           (UBP6) similar to GI:11993465
          Length = 482

 Score = 36.3 bits (80), Expect = 0.013
 Identities = 18/60 (30%), Positives = 35/60 (58%)
 Frame = +2

Query: 53  MLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPNLKIMMMGSLEEAIQGAQ 232
           + K  + + TGV PERQK++    +G +  D+   + + +K   K+MMMG+ +E ++  +
Sbjct: 26  VFKAQLYDLTGVPPERQKIM---VKGGLLKDDGDWAAIGVKDGQKLMMMGTADEIVKAPE 82


>At2g30100.1 68415.m03663 ubiquitin family protein low similarity to
           SP|Q9UQ13 Leucine-rich repeat protein SHOC-2
           (Ras-binding protein Sur-8) {Homo sapiens}; contains
           Pfam profiles PF00240: Ubiquitin family, PF01535: PPR
           repeat, PF00560: Leucine Rich Repeat
          Length = 897

 Score = 35.9 bits (79), Expect = 0.018
 Identities = 21/58 (36%), Positives = 31/58 (53%)
 Frame = +2

Query: 32  SPSDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPNLKIMMMGS 205
           SP  +V  LK  ++  T V P  QKL+   F+GKV  +  TL   ++    K+M+M S
Sbjct: 552 SPDCTVKDLKSQLQPITNVLPRGQKLI---FKGKVLVETSTLKQSDVGSGAKLMLMAS 606


>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
           polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
           thaliana] (Genetics 139 (2), 921-939 (1995))
          Length = 631

 Score = 34.3 bits (75), Expect = 0.054
 Identities = 19/62 (30%), Positives = 34/62 (54%)
 Frame = +2

Query: 38  SDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPNLKIMMMGSLEEA 217
           SDS+  +K  I+N  G+  ++Q+L+   F GK   D  TL+D N++    + ++  L   
Sbjct: 22  SDSIHNVKAKIQNKEGIPLDQQRLI---FAGKQLEDGLTLADYNIQKESTLHLVLRLRGG 78

Query: 218 IQ 223
           +Q
Sbjct: 79  MQ 80


>At1g53950.1 68414.m06145 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 216

 Score = 33.1 bits (72), Expect = 0.13
 Identities = 19/60 (31%), Positives = 33/60 (55%)
 Frame = +2

Query: 20  IPEFSPSDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCTLSDLNLKPNLKIMMM 199
           I E   SD++  LK  I +  G   ++Q L ++   G    +N T+SD N+KP+  +++M
Sbjct: 154 ILEVKGSDTIKKLKSMIHDQGGPPVDQQDLNHL---GTKLKNNATISDYNIKPDANLVIM 210


>At1g64470.1 68414.m07309 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 213

 Score = 28.7 bits (61), Expect = 2.7
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = +2

Query: 38  SDSVAMLKIAIENATGVRPERQKLLNIKFQGKVATDNCT 154
           SDS+A +K+ I+   G R  RQKL+   F G+    N +
Sbjct: 53  SDSIAEVKLRIQTCNGFRVRRQKLV---FSGRELARNAS 88


>At5g66130.1 68418.m08331 cell cycle checkpoint protein-related weak
           similarity to cell cycle checkpoint protein RAD17 [Homo
           sapiens] GI:4102916
          Length = 599

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 13/54 (24%), Positives = 29/54 (53%)
 Frame = +1

Query: 49  SDVENSYRKCHGRSSRKTKAAQYKISGESGNRQLYIIRFESQAKFEDYDDGLSG 210
           S+ ++  R   G ++    + + ++SG S  ++   +  + +  FED+D+ LSG
Sbjct: 24  SNAKSKPRSSAGTATNPRASKRARLSGASATQEDSSLVDKIRLSFEDFDEALSG 77


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,926,650
Number of Sequences: 28952
Number of extensions: 169247
Number of successful extensions: 456
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 454
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)