Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_N17
(348 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 64 2e-11
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 63 4e-11
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 62 1e-10
At5g13330.1 68418.m01533 AP2 domain-containing transcription fac... 28 2.0
At5g36820.1 68418.m04411 F-box family protein contains Pfam PF00... 27 2.6
At5g36730.1 68418.m04395 F-box family protein contains Pfam PF00... 27 2.6
At1g57680.2 68414.m06546 expressed protein 27 2.6
At1g57680.1 68414.m06545 expressed protein 27 2.6
At1g79800.1 68414.m09316 plastocyanin-like domain-containing pro... 27 3.4
At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin... 27 3.4
At5g65820.1 68418.m08282 pentatricopeptide (PPR) repeat-containi... 26 6.0
At1g15240.1 68414.m01824 phox (PX) domain-containing protein wea... 26 6.0
At4g01640.1 68417.m00213 hypothetical protein 26 7.9
At3g07840.1 68416.m00959 polygalacturonase, putative / pectinase... 26 7.9
At2g07676.1 68415.m00907 expressed protein 26 7.9
At2g07240.1 68415.m00831 Ulp1 protease family protein contains P... 26 7.9
At1g57780.1 68414.m06556 heavy-metal-associated domain-containin... 26 7.9
>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase A SP:P13723 from
[Dictyostelium discoideum]
Length = 541
Score = 64.5 bits (150), Expect = 2e-11
Identities = 29/53 (54%), Positives = 39/53 (73%)
Frame = +3
Query: 3 DTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAY 161
DTSRHYL + I + I++M+ K+NVLHWHIVD+QSFP + +P L + GAY
Sbjct: 193 DTSRHYLPIDVIKQIIESMSFAKLNVLHWHIVDEQSFPLETPTYPNLWK-GAY 244
Score = 47.6 bits (108), Expect = 2e-06
Identities = 18/40 (45%), Positives = 24/40 (60%)
Frame = +2
Query: 176 YTKRDIEIVVKHAAERGVRVLPEFDVPGHTSSWGVAFPSI 295
YT D +V+ A RG+ V+ E DVPGH SWG +P +
Sbjct: 250 YTVEDASEIVRFAKMRGINVMAEVDVPGHAESWGTGYPDL 289
>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
contains Pfam PF00728: Glycosyl hydrolase family 20,
catalytic domain; contains Pfam PF02838: Glycosyl
hydrolase family 20, domain 2; similar to
Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
(N-acetyl-beta- glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase B)
(Swiss-Prot:P07686) [Homo sapiens]
Length = 535
Score = 63.3 bits (147), Expect = 4e-11
Identities = 31/53 (58%), Positives = 35/53 (66%)
Frame = +3
Query: 3 DTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAY 161
DTSRHYL L I ID+M K+NVLHWHIVD QSFP +P+L GAY
Sbjct: 190 DTSRHYLPLPVIKNVIDSMTYAKLNVLHWHIVDTQSFPLEIPSYPKLWN-GAY 241
Score = 48.0 bits (109), Expect = 2e-06
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = +2
Query: 176 YTKRDIEIVVKHAAERGVRVLPEFDVPGHTSSWGVAFPSI 295
YT D +V +A RG+ VL E DVPGH SWG +P++
Sbjct: 247 YTFEDAAEIVNYARRRGIHVLAEIDVPGHALSWGKGYPAL 286
>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase precursor SP:P43077 from
[Candida albicans]
Length = 580
Score = 62.1 bits (144), Expect = 1e-10
Identities = 27/55 (49%), Positives = 35/55 (63%)
Frame = +3
Query: 3 DTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYHP 167
DTSR+Y + I++ I AM+ANK+NV HWHI D QSFP P L+ G+ P
Sbjct: 179 DTSRNYYGVDDIMRTIKAMSANKLNVFHWHITDSQSFPLVLPSEPSLAAKGSLGP 233
Score = 61.3 bits (142), Expect = 2e-10
Identities = 26/46 (56%), Positives = 32/46 (69%)
Frame = +2
Query: 176 YTKRDIEIVVKHAAERGVRVLPEFDVPGHTSSWGVAFPSILTKCYN 313
YT D+ +V++ E GVRVLPE D PGHT SWG A+P I+T C N
Sbjct: 237 YTPEDVSKIVQYGFEHGVRVLPEIDTPGHTGSWGEAYPEIVT-CAN 281
>At5g13330.1 68418.m01533 AP2 domain-containing transcription factor
family protein similar to AP2 domain containing protein
RAP2.6, Arabidopsis thaliana, EMBL:AF003099; contains
Pfam profile PF00847: AP2 domain
Length = 212
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/51 (25%), Positives = 27/51 (52%)
Frame = -1
Query: 195 SISRLVYTNVDDKLRVDLVRETFHCDKENSDHRRYANGVRSFYSRPWHRYS 43
++SR++ D ++ +L + H ++ RR+ GVR RPW +++
Sbjct: 4 ALSRVIENPTDPPVKQELDKSDQHQPDQDQPRRRHYRGVR---QRPWGKWA 51
>At5g36820.1 68418.m04411 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 410
Score = 27.5 bits (58), Expect = 2.6
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = -1
Query: 189 SRLVYTNVDDKLRVDLVRETFHCD 118
++L+ N DD++RVD + + FHCD
Sbjct: 93 AKLIRLN-DDRVRVDDICKVFHCD 115
>At5g36730.1 68418.m04395 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 410
Score = 27.5 bits (58), Expect = 2.6
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = -1
Query: 189 SRLVYTNVDDKLRVDLVRETFHCD 118
++L+ N DD++RVD + + FHCD
Sbjct: 93 AKLIRLN-DDRVRVDDICKVFHCD 115
>At1g57680.2 68414.m06546 expressed protein
Length = 362
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -1
Query: 222 LSAACFTTISISRLVYTNVDDKLRVDLVRETFHCDKENSDHR 97
LS CF +SI LVY V D + + +R+T D ++ R
Sbjct: 277 LSLFCFCVVSICLLVYFPVSDSMALRGLRDTDDEDTAVTEER 318
>At1g57680.1 68414.m06545 expressed protein
Length = 362
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -1
Query: 222 LSAACFTTISISRLVYTNVDDKLRVDLVRETFHCDKENSDHR 97
LS CF +SI LVY V D + + +R+T D ++ R
Sbjct: 277 LSLFCFCVVSICLLVYFPVSDSMALRGLRDTDDEDTAVTEER 318
>At1g79800.1 68414.m09316 plastocyanin-like domain-containing
protein
Length = 192
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 39 LKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYH 164
L+N A+ ++ + +HI D SF Y + E+ + G YH
Sbjct: 46 LQNDSAVYSHWASSNRFHIGDSLSFVYDKDSVMEVDKWGFYH 87
>At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase
1 [Arabidopsis thaliana] Swiss-Prot:Q42525
Length = 493
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 90 ANGVRSFYSRPWHRYSLVSSITINSAWMCP 1
A G +F SRP Y ++S++ NSA CP
Sbjct: 13 ALGSFTFSSRPRSNYIVMSAVRSNSASTCP 42
>At5g65820.1 68418.m08282 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 637
Score = 26.2 bits (55), Expect = 6.0
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -1
Query: 159 KLRVDLVRETFHCDKENSDHRRYANGV 79
K R DL+ +FH + D+ R +NG+
Sbjct: 32 KSRFDLIHRSFHVSRALEDNFRRSNGI 58
>At1g15240.1 68414.m01824 phox (PX) domain-containing protein weak
similarity to SP|Q9Y5W8 Sorting nexin 13 {Homo sapiens};
contains Pfam profiles PF00787: PX domain, PF02194: PXA
domain
Length = 706
Score = 26.2 bits (55), Expect = 6.0
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +2
Query: 221 RGVRVLPEFDV---PGHTSSWGVAFPSILTKCYNGEEYV 328
R ++V PE+ + P H S GV P I +C +EY+
Sbjct: 659 RRLKVFPEYKLHLPPKHFLSTGVDIPVIQERCVLLDEYI 697
>At4g01640.1 68417.m00213 hypothetical protein
Length = 300
Score = 25.8 bits (54), Expect = 7.9
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = +3
Query: 81 LHWHIVDDQSFPYHSEKFPELSRLGAYHPH 170
LHW + SF H+E F +S+ H H
Sbjct: 154 LHWLTTNVLSFDLHTETFQVVSKAPFLHHH 183
>At3g07840.1 68416.m00959 polygalacturonase, putative / pectinase,
putative strong similarity to polygalacturonase PGA3
[Arabidopsis thaliana] GI:3152948; contains
non-consensus AA acceptor splice site at exon 3
Length = 401
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 3/40 (7%)
Frame = -1
Query: 255 GTSNSGKTRTPLSAACFTT---ISISRLVYTNVDDKLRVD 145
GT N + +T SAAC TT I ++ NV + + +D
Sbjct: 262 GTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILID 301
>At2g07676.1 68415.m00907 expressed protein
Length = 118
Score = 25.8 bits (54), Expect = 7.9
Identities = 11/40 (27%), Positives = 21/40 (52%)
Frame = +3
Query: 27 LSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELS 146
+SK +K I ++ W+I+DD ++ E+F L+
Sbjct: 39 ISKYIKGILKSRLSRREQSRWNIIDDTTYMAFFEEFASLN 78
>At2g07240.1 68415.m00831 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 928
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/36 (33%), Positives = 16/36 (44%)
Frame = -1
Query: 132 TFHCDKENSDHRRYANGVRSFYSRPWHRYSLVSSIT 25
T H K DH + ++ F S PW R S +T
Sbjct: 83 TSHYPKIIKDHAEMSEDLQGFLSYPWGRLSFEMMMT 118
>At1g57780.1 68414.m06556 heavy-metal-associated domain-containing
protein low similarity to myosin-like antigen GI:159877
Onchocerca volvulus; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 264
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +2
Query: 194 EIVVKHAAERGVRVLP-EFDVPGHTSSWGVAFPSILTKCYNGEEYV 328
EIV ++A + ++ EFD+P + F +++KC E YV
Sbjct: 120 EIVPQNADKPETSIMEVEFDIPFLCEKYEKDFGKVISKCTGVETYV 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,652,947
Number of Sequences: 28952
Number of extensions: 148242
Number of successful extensions: 386
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 375
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 386
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 429398688
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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