Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_N04
(345 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34555.1 68417.m04910 40S ribosomal protein S25, putative 93 5e-20
At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E) ribo... 91 1e-19
At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B) 91 1e-19
At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A) 85 2e-17
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 31 0.16
At1g69430.1 68414.m07978 hypothetical protein 28 1.5
At2g17250.1 68415.m01992 expressed protein weak similarity to Ri... 28 1.9
At1g48180.1 68414.m05378 expressed protein ; expression supporte... 27 2.5
At2g22180.1 68415.m02634 hydroxyproline-rich glycoprotein family... 27 3.4
At1g24706.1 68414.m03104 expressed protein 27 3.4
At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containi... 27 4.4
At5g37570.1 68418.m04526 pentatricopeptide (PPR) repeat-containi... 26 5.9
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 26 7.7
At5g10660.1 68418.m01234 calmodulin-binding protein-related cont... 26 7.7
At3g28345.1 68416.m03541 ABC transporter family protein similar ... 26 7.7
At1g51960.1 68414.m05857 calmodulin-binding family protein conta... 26 7.7
>At4g34555.1 68417.m04910 40S ribosomal protein S25, putative
Length = 108
Score = 93.1 bits (221), Expect = 5e-20
Identities = 43/72 (59%), Positives = 55/72 (76%)
Frame = +1
Query: 112 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 291
+NN VLFD+ TY+KL E P++KLITP+++S+RL+I GSLARRA+ EL KG I+ V H
Sbjct: 37 VNNMVLFDQATYDKLLSEAPKFKLITPSILSDRLRINGSLARRAIRELMAKGTIRMVSAH 96
Query: 292 HGQVIYTRATKG 327
Q IYTRAT G
Sbjct: 97 SSQQIYTRATHG 108
>At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E)
ribosomal protein S25, Lycopersicon esculentum,
PIR2:S40089
Length = 108
Score = 91.5 bits (217), Expect = 1e-19
Identities = 41/70 (58%), Positives = 55/70 (78%)
Frame = +1
Query: 112 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 291
+NN VLFD+ TY+KL E P++KLITP+++S+R++I GSLARRA+ EL KG+I+ V H
Sbjct: 37 VNNMVLFDQATYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAKGVIRMVAAH 96
Query: 292 HGQVIYTRAT 321
Q IYTRAT
Sbjct: 97 SSQQIYTRAT 106
>At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B)
Length = 108
Score = 91.5 bits (217), Expect = 1e-19
Identities = 42/70 (60%), Positives = 55/70 (78%)
Frame = +1
Query: 112 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 291
+NN VLFD+ TY+KL E P++KLITP+++S+R++I GSLARRA+ EL KGLI+ V H
Sbjct: 37 VNNMVLFDQGTYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAKGLIRMVSAH 96
Query: 292 HGQVIYTRAT 321
Q IYTRAT
Sbjct: 97 SSQQIYTRAT 106
>At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A)
Length = 125
Score = 84.6 bits (200), Expect = 2e-17
Identities = 39/70 (55%), Positives = 53/70 (75%)
Frame = +1
Query: 112 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 291
+NN VLFD+ TY+KL E P++KLITP+++S+RL+I GSLAR+A+ +L KG I+ V H
Sbjct: 53 VNNMVLFDQATYDKLMSEAPKFKLITPSILSDRLRINGSLARKAIRDLMVKGTIRMVSTH 112
Query: 292 HGQVIYTRAT 321
Q I TRAT
Sbjct: 113 SSQQINTRAT 122
>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
CAF protein [Arabidopsis thaliana] GI:6102610; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain, PF03368: Domain of
unknown function, PF00636: RNase3 domain, PF00035:
Double-stranded RNA binding motif
Length = 1676
Score = 31.5 bits (68), Expect = 0.16
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -1
Query: 291 VLDYLFD*TFISKFDKCSPGKGASDLQPFR-DNSGCDQLVLWYFLIQFLICGFVKQHLVV 115
VLDYL F + F K PG+ +DL+ +N + + + L +FL C + H V+
Sbjct: 1310 VLDYLMTSYFFTVFPKLKPGQ-LTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVI 1368
Query: 114 *LVTDF 97
T+F
Sbjct: 1369 EDYTNF 1374
>At1g69430.1 68414.m07978 hypothetical protein
Length = 350
Score = 28.3 bits (60), Expect = 1.5
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = -3
Query: 154 VSHMWVCQTTLGCLTCHGLFLLTTSSSW 71
++++W+C + CLT +FL+ SS+
Sbjct: 172 ITYLWICTVIVVCLTSFCVFLVAVCSSF 199
>At2g17250.1 68415.m01992 expressed protein weak similarity to
Ribosome biogenesis protein MAK21 (Swiss-Prot:Q12176)
[Saccharomyces cerevisiae]
Length = 577
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/36 (30%), Positives = 24/36 (66%)
Frame = +1
Query: 115 NNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIR 222
+++ + +KPT +K E L++PA +S+R+K++
Sbjct: 245 SDESISEKPTDKKKKTEKGDSTLLSPATISKRMKLK 280
>At1g48180.1 68414.m05378 expressed protein ; expression supported
by MPSS
Length = 239
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -2
Query: 227 EPRIFNLSETTAGVISLYCGTSLYSFSYVGL 135
+P I L + + + +YCGTS SYVGL
Sbjct: 144 KPHI-ELQQDASAITGIYCGTSGEPASYVGL 173
>At2g22180.1 68415.m02634 hydroxyproline-rich glycoprotein family
protein
Length = 291
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = -1
Query: 192 GCDQLVLWYFLIQFLICGFVKQHLVV*LVTDFSF*P 85
GC + +LW+F+I +I GF+ ++ L+ F+F P
Sbjct: 115 GCSKRLLWFFII-LVIFGFLLGAII--LILHFAFNP 147
>At1g24706.1 68414.m03104 expressed protein
Length = 1781
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -2
Query: 113 NLSRTFPFDHFFFLALPPPDP 51
+L ++ P DHF LPPP P
Sbjct: 1562 SLEKSHPDDHFHSQGLPPPPP 1582
>At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 1028
Score = 26.6 bits (56), Expect = 4.4
Identities = 19/53 (35%), Positives = 24/53 (45%)
Frame = -1
Query: 315 SCVDHLPMVLDYLFD*TFISKFDKCSPGKGASDLQPFRDNSGCDQLVLWYFLI 157
S + HL LD L T I + KC KG+S Q F + +V W LI
Sbjct: 756 SLIFHLAHDLDELTSNTLIDMYAKCGDMKGSS--QVFDEMRRRSNVVSWNSLI 806
>At5g37570.1 68418.m04526 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 550
Score = 26.2 bits (55), Expect = 5.9
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
Frame = +1
Query: 151 KLYKEVPQYKLITPAVVSERLKIR--GSLARRALVELRDKGLIKQVV 285
KL++E+PQ L++ + E + I GS A R ++ D+G++ V
Sbjct: 361 KLFEEMPQRDLVSYCSMMEGMAIHGCGSEAIRLFEKMVDEGIVPDEV 407
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 25.8 bits (54), Expect = 7.7
Identities = 11/21 (52%), Positives = 14/21 (66%), Gaps = 1/21 (4%)
Frame = -2
Query: 197 TAGVISLY-CGTSLYSFSYVG 138
T G I LY CG + Y FS++G
Sbjct: 25 TPGKIGLYVCGITAYDFSHIG 45
>At5g10660.1 68418.m01234 calmodulin-binding protein-related
contains weak similarity to calmodulin-binding proteins
Length = 407
Score = 25.8 bits (54), Expect = 7.7
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = +3
Query: 30 EDPKEEGGIRWRQSQEEEVVKRXXXXXXXXXXXXXXTHI*ETV 158
E+PKE I S+EEE VK+ T + E V
Sbjct: 270 EEPKEPENIEENNSEEEEEVKKKSDDEENSETVATTTDMNEAV 312
>At3g28345.1 68416.m03541 ABC transporter family protein similar to
P-glycoprotein [Arabidopsis thaliana] GI:3849833;
contains Pfam profiles PF00005: ABC transporter,
PF00664: ABC transporter transmembrane region
Length = 1240
Score = 25.8 bits (54), Expect = 7.7
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -2
Query: 167 TSLYSFSYVGLSNNTWLFNLSRTFPF 90
T +Y+ S+VGL+ ++L N+S+ + F
Sbjct: 712 TRIYALSFVGLAVLSFLINISQHYNF 737
>At1g51960.1 68414.m05857 calmodulin-binding family protein contains
IQ calmodulin-binding motif, Pfam:PF00612
Length = 351
Score = 25.8 bits (54), Expect = 7.7
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +1
Query: 193 AVVSERLKIRGSLARRALVELRDKGLIKQVVQHHGQVIYTRA 318
A V + RGSLAR+AL L KG++K G ++ RA
Sbjct: 102 AAVKIQKVFRGSLARKALRAL--KGIVKLQALVRGYLVRKRA 141
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,762,916
Number of Sequences: 28952
Number of extensions: 119625
Number of successful extensions: 337
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 337
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 419412672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -