SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_M20
         (540 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3F10.12c |||transcription factor |Schizosaccharomyces pombe|...    38   0.001
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S...    32   0.047
SPAC222.06 |mak16||nuclear HMG-like acidic protein Mak16|Schizos...    30   0.25 
SPCC330.02 |rhp7|SPCC613.14|Rad7 homolog Rhp7|Schizosaccharomyce...    29   0.44 
SPAC22F3.02 |atf31||transcription factor Atf31|Schizosaccharomyc...    29   0.58 
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    28   0.77 
SPAC664.08c |||traub transciption factor family protein |Schizos...    28   0.77 
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S...    28   1.0  
SPBC337.06c |cwf15||complexed with Cdc5 protein Cwf15 |Schizosac...    28   1.0  
SPBC146.03c |cut3|smc4, smc4|condensin subunit Cut3|Schizosaccha...    27   2.3  
SPCC126.09 |||vacuolar membrane zinc transporter |Schizosaccharo...    27   2.3  
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom...    27   2.3  
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos...    26   3.1  
SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces pom...    26   3.1  
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces...    26   3.1  
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr...    26   4.1  
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe...    26   4.1  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    25   7.2  
SPBC4B4.02c |nca2||mitochondrial protein Nca2 |Schizosaccharomyc...    25   7.2  
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom...    25   7.2  
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom...    25   7.2  
SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces...    25   9.5  
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac...    25   9.5  
SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo...    25   9.5  
SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schi...    25   9.5  
SPAC22F8.09 |rrp16|nop53|rRNA processing protein Rrp16 |Schizosa...    25   9.5  

>SPAC3F10.12c |||transcription factor |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 201

 Score = 37.5 bits (83), Expect = 0.001
 Identities = 18/56 (32%), Positives = 34/56 (60%)
 Frame = +3

Query: 312 RREAHTQAEQKRRDAIKKGYDSLQELVPTCQQTDASGYKHSKAAVLQKSIDYIQYL 479
           +R +H + E++RR+AI +G   L  +VP C++        +K ++LQ++  YI+ L
Sbjct: 89  KRLSHKEVERRRREAISEGIKELANIVPGCEK--------NKGSILQRTAQYIRSL 136


>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
           Tor2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2337

 Score = 32.3 bits (70), Expect = 0.047
 Identities = 20/49 (40%), Positives = 30/49 (61%), Gaps = 2/49 (4%)
 Frame = +1

Query: 196 VAVGLYKTS--IKLRRLLIKIQKTKMTVEIIRHRHLVLKKGEEKLTPKQ 336
           + +G+ KT   I +RR  IK+  T   ++  RHR  VL+KG EK+ P+Q
Sbjct: 735 ILIGITKTEQDITIRRETIKLIGTLGALDPNRHR--VLEKGTEKVVPEQ 781


>SPAC222.06 |mak16||nuclear HMG-like acidic protein
           Mak16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 302

 Score = 29.9 bits (64), Expect = 0.25
 Identities = 17/73 (23%), Positives = 35/73 (47%)
 Frame = +3

Query: 168 DICNMYSRCGSSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKR 347
           +I ++    GS  S++        S+++  DED+  DNK      ++R+ +   ++ +KR
Sbjct: 222 EISDLEDWLGSDQSMETSESEEEESSESESDEDEDEDNKG---KIRKRKTDDAKKSRKKR 278

Query: 348 RDAIKKGYDSLQE 386
              I   Y+  +E
Sbjct: 279 APHIHIEYEQERE 291


>SPCC330.02 |rhp7|SPCC613.14|Rad7 homolog Rhp7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 563

 Score = 29.1 bits (62), Expect = 0.44
 Identities = 16/52 (30%), Positives = 25/52 (48%)
 Frame = +3

Query: 198 SSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKRRD 353
           S+G+   + QTP+S  +N EDE+            K  RR    Q ++K+ D
Sbjct: 44  STGTESEVIQTPTSVEENNEDENSMSTTTIEIPVVK--RRNLRNQKKKKKTD 93


>SPAC22F3.02 |atf31||transcription factor Atf31|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 209

 Score = 28.7 bits (61), Expect = 0.58
 Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
 Frame = +3

Query: 234 SSSNQNTEDEDDSGDNKASALSFKERR--REAHTQAEQKRRDAIKKGYDSLQELV 392
           +S N  + + DDSGD   S L+  ++   +  + QA QK R   KK   +LQ+ V
Sbjct: 99  ASHNSPSRELDDSGDENTSKLTGTKQSMLKARNRQAAQKCRIKKKKYLQTLQDQV 153


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1067

 Score = 28.3 bits (60), Expect = 0.77
 Identities = 15/55 (27%), Positives = 28/55 (50%)
 Frame = +3

Query: 231 PSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKRRDAIKKGYDSLQELVP 395
           PS+  +  +D DD   N  S+ S K   + + TQA + + D  + G +  ++ +P
Sbjct: 87  PSTDKKKKKDNDDFKQNLKSS-SNKTEEKYSATQASKSKNDEFELGGEENEDEMP 140


>SPAC664.08c |||traub transciption factor family protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 452

 Score = 28.3 bits (60), Expect = 0.77
 Identities = 16/66 (24%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
 Frame = +3

Query: 198 SSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKRRDAI-KKGYD 374
           +SGS+ +   +PS    + EDE+D+        S  E    +    +  +  AI +K  D
Sbjct: 81  NSGSLNSQSSSPSEEEDSEEDENDAVSEDHENFSSSEASSISEENEDDDQSSAIPEKDMD 140

Query: 375 SLQELV 392
            L++++
Sbjct: 141 RLKKII 146


>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1944

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 3/57 (5%)
 Frame = +3

Query: 222  HQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQ---KRRDAIKKGYDSLQ 383
            +  P   N +T DED S   + S+       REA++ A +   +RR      +DSL+
Sbjct: 974  YSNPDDKNTSTSDEDTSESEEESSNGLFSLAREANSHASKSLPQRRQIQFLDFDSLK 1030


>SPBC337.06c |cwf15||complexed with Cdc5 protein Cwf15
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 265

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
 Frame = +3

Query: 213 QNIHQTPSSSNQNTEDEDDSGD--NKASALSFKERRREAHTQ 332
           Q + +    ++++  D DDS D  NK S +S K R+ E+++Q
Sbjct: 105 QELLRQTLEADEDASDSDDSVDSSNKNSEVSIKRRKTESNSQ 146


>SPBC146.03c |cut3|smc4, smc4|condensin subunit
            Cut3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1324

 Score = 26.6 bits (56), Expect = 2.3
 Identities = 16/79 (20%), Positives = 37/79 (46%)
 Frame = +3

Query: 162  ITDICNMYSRCGSSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQ 341
            +T+I  +  +    G I+  ++   S   +  ++     +K + +SFK+++ E  +Q+ Q
Sbjct: 916  VTEIKTLQDKIMEIGGIR--YRIQKSKVDDLHEQLKFVKDKLNKMSFKKKKNEQRSQSFQ 973

Query: 342  KRRDAIKKGYDSLQELVPT 398
                 +   YD+  E + T
Sbjct: 974  VELSNLTSEYDTTTESIAT 992


>SPCC126.09 |||vacuolar membrane zinc transporter
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 418

 Score = 26.6 bits (56), Expect = 2.3
 Identities = 13/39 (33%), Positives = 19/39 (48%)
 Frame = -2

Query: 365 FLNGISSFLFCLGVSFSSPFFKTKCRCLIISTVIFVFCI 249
           FL+    F  C G+   + F  T C+  I+S  I  FC+
Sbjct: 9   FLHLSKEFYDCCGLFLLTFFLLTTCQLRILSLSINAFCV 47


>SPCC1223.08c |dfr1||dihydrofolate reductase
           Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 461

 Score = 26.6 bits (56), Expect = 2.3
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = -2

Query: 260 VFCILIRRRRSLMDVLYRPTATTPRVHVAYISD 162
           VF    R  +   D  Y P  TTP +H+A  SD
Sbjct: 144 VFVGGFRAEKPEFDHFYNPKLTTPSLHIAGTSD 176


>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 482

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = +3

Query: 222 HQTPSSSNQNTEDEDDSGDNKASALS 299
           + TP S  +  ED+D +G NK +A S
Sbjct: 365 NNTPMSEKRKREDDDANGPNKFAARS 390


>SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 459

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = +3

Query: 300 FKERRREAHTQAEQKRRDAIKKGYDSLQELV 392
           +KE  R  HT   +  ++A++K  D L +LV
Sbjct: 4   YKELARRLHTLQSKNEKEALEKQIDFLDKLV 34


>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 446

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 14/53 (26%), Positives = 27/53 (50%)
 Frame = +3

Query: 195 GSSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKRRD 353
           G SG +  +      S+ ++++ D+  D+    LS  ERR     + +QK++D
Sbjct: 378 GYSGYVAGLSSRDPDSDFDSDNVDEYIDDHKRGLSSTERRDLDRVRDKQKKQD 430


>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 872

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 15/86 (17%), Positives = 36/86 (41%)
 Frame = +1

Query: 100 LFVNGTYFNTIDKILRFQLYKSLIYATCTLGVVAVGLYKTSIKLRRLLIKIQKTKMTVEI 279
           L VN +       + +  LY+ ++     LG+    L+  S   +     + +   T+E+
Sbjct: 133 LEVNSSVSLENTNVCKASLYRFMLMCKNELGLTDAALFSISEIYKPSTAPLVRALQTIEL 192

Query: 280 IRHRHLVLKKGEEKLTPKQNRNEEMP 357
           +  ++ V    +   TP  + ++ +P
Sbjct: 193 LLKKYEVSNTTKSSSTPSPSTDDNVP 218


>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 317

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 15/64 (23%), Positives = 28/64 (43%)
 Frame = +3

Query: 186 SRCGSSGSIQNIHQTPSSSNQNTEDEDDSGDNKASALSFKERRREAHTQAEQKRRDAIKK 365
           +R  SS  I +   TPSSS+ ++     S  +K+S+ S       + +++      + K 
Sbjct: 139 TRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSSSSSSSKSSSSSSSSSKS 198

Query: 366 GYDS 377
              S
Sbjct: 199 SSSS 202


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = +1

Query: 214 KTSIKLRRLLIKIQKTKMTVEIIRHRHLVLKKGEEKLTPKQN 339
           KT ++  RL  +++KTK   E+    H++      ++T KQN
Sbjct: 755 KTVLEQHRLS-EMRKTKKQTELTNSAHVIKPSPTPQITVKQN 795


>SPBC4B4.02c |nca2||mitochondrial protein Nca2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 573

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 10/17 (58%), Positives = 15/17 (88%)
 Frame = +1

Query: 229 LRRLLIKIQKTKMTVEI 279
           +R LLI++QKTK+ VE+
Sbjct: 419 VRTLLIQLQKTKVDVEV 435


>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
           pombe|chr 1||Partial|Manual
          Length = 1887

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 14/46 (30%), Positives = 23/46 (50%), Gaps = 4/46 (8%)
 Frame = +3

Query: 243 NQNTEDEDDSGDNKASALSFKERRRE----AHTQAEQKRRDAIKKG 368
           N   E+E++ G+ K  A + KER  +       +  Q+RR+   KG
Sbjct: 294 NGGEEEEEEEGEEKEDAQNIKERMVDFCFSKFMEKNQQRREQQDKG 339


>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase
           Tlh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1919

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 14/46 (30%), Positives = 23/46 (50%), Gaps = 4/46 (8%)
 Frame = +3

Query: 243 NQNTEDEDDSGDNKASALSFKERRRE----AHTQAEQKRRDAIKKG 368
           N   E+E++ G+ K  A + KER  +       +  Q+RR+   KG
Sbjct: 294 NGGEEEEEEEGEEKEDAQNIKERMVDFCFSKFMEKNQQRREQQDKG 339


>SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1441

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 11/38 (28%), Positives = 20/38 (52%)
 Frame = +1

Query: 325 TPKQNRNEEMPLRKDMIHFKSWFLPVNKLMHQAISTVK 438
           +P+Q     +PLRKD + F    L + +     I+T++
Sbjct: 251 SPEQTSTVTLPLRKDTVLFSLVTLDLEQRASAVITTIQ 288


>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +3

Query: 249 NTEDEDDSGDNKASALSFKERRREAHTQAEQKRRDAIKKGYDSLQELVPTCQQTDASG 422
           N ++ D+S   K   L  KER  E     E++RRD  +K +D+  + +   ++   SG
Sbjct: 303 NAKELDESRRKKIELLEKKERDEEQF--LEKERRDTARK-WDNQGDFIRNMRKEIYSG 357


>SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog
           Rhp16|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 963

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 10/27 (37%), Positives = 15/27 (55%)
 Frame = +3

Query: 228 TPSSSNQNTEDEDDSGDNKASALSFKE 308
           +P  SN++   +DD  D  AS +  KE
Sbjct: 230 SPEESNESEFIDDDESDEVASIIDIKE 256


>SPAC1B3.10c |||SEL1 repeat protein, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 680

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
 Frame = +2

Query: 8   EVDTY-YYNDIILFFITYLGKMGYAKKNLLIFFL*MERTLIQ*IRFYVFNF 157
           E+D Y Y+++  ++++ Y  +MGYA   +   +L  E   +    F  FN+
Sbjct: 489 EIDEYGYFHNSFVYYL-YAAQMGYALAEINAAYLMDENKFLINSVFRYFNY 538


>SPAC22F8.09 |rrp16|nop53|rRNA processing protein Rrp16
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 419

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 19/79 (24%), Positives = 31/79 (39%), Gaps = 4/79 (5%)
 Frame = +3

Query: 216 NIHQTPSSSNQNTEDEDDSGDNKASALS----FKERRREAHTQAEQKRRDAIKKGYDSLQ 383
           +I     S ++  E    S  NK    S     ++RR E     EQK+ + + +  D   
Sbjct: 249 SIDSDDQSEHEQAETPIPSSKNKRKTRSQRNKIRQRREEELRLLEQKKNEELLRTIDKAP 308

Query: 384 ELVPTCQQTDASGYKHSKA 440
            +    QQ D +    +KA
Sbjct: 309 AISKKLQQKDKAEKFSNKA 327


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,119,032
Number of Sequences: 5004
Number of extensions: 42540
Number of successful extensions: 211
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -