BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_M02
(586 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,... 29 3.0
At3g57420.1 68416.m06393 expressed protein contains Pfam domain ... 28 5.3
At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine-... 27 9.2
>At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,
putative / stearoyl-ACP desaturase, putative similar to
Acyl-[acyl-carrier protein] desaturase from Spinacia
oleracea SP|P28645, Olea europaea SP|Q43593; contains
Pfam profile PF03405 Fatty acid desaturase
Length = 396
Score = 28.7 bits (61), Expect = 3.0
Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 3/98 (3%)
Frame = +3
Query: 123 PNHVSKSITISASWSIENNINHYKNETAVKIWILMKKQNWLLPLSVPF-SPLNPTHQFEA 299
P H+ S T + N + H T K+ I +NW + + P+ + Q +
Sbjct: 36 PRHLQVSKTFRPIKEVSNQVTH--TITQEKLEIFKSMENWAQENLLSYLKPVETSWQPQ- 92
Query: 300 VIMFNVLYSAKDYDTFYKTTVYMKDRVNQ--DLYIYVL 407
+ L KD D FY+ ++DR + D Y VL
Sbjct: 93 ----DFLPETKDEDRFYEQVKELRDRTKEIPDDYFVVL 126
>At3g57420.1 68416.m06393 expressed protein contains Pfam domain
PF03385: Protein of unknown function, DUF288
Length = 765
Score = 27.9 bits (59), Expect = 5.3
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 336 YDTFYKTTVYMKDRVNQDLYIYVLSTLHIHR 428
Y +KT V + R N DLY+ HI++
Sbjct: 534 YGRIFKTVVILSSRKNSDLYVQEAKLDHIYK 564
>At3g55400.1 68416.m06153 methionyl-tRNA synthetase /
methionine--tRNA ligase / MetRS (cpMetRS) identical to
methionyl-tRNA synthetase MEtRS [Arabidopsis thaliana]
GI:2266985
Length = 616
Score = 27.1 bits (57), Expect = 9.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 336 YDTFYKTTVYMKDRVNQDLYIYVLSTLHIHRSDLEG 443
YD F +TT + + ++ Y V + I+R+D EG
Sbjct: 155 YDKFIRTTDPKHEAIVKEFYARVFANGDIYRADYEG 190
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,906,450
Number of Sequences: 28952
Number of extensions: 267765
Number of successful extensions: 607
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 607
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1151426952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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