Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_L03
(424 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A1IRV2 Cluster: Putative uncharacterized protein; n=2; ... 35 0.60
UniRef50_Q1WMS8 Cluster: Putative uncharacterized protein UP10; ... 35 0.60
UniRef50_P85216 Cluster: Anionic antimicrobial peptide 2; n=1; G... 34 1.0
UniRef50_A6LW08 Cluster: Signal transduction histidine kinase, L... 33 1.8
UniRef50_A2DE23 Cluster: Putative uncharacterized protein; n=1; ... 33 1.8
UniRef50_Q5KFM1 Cluster: Putative uncharacterized protein; n=1; ... 33 2.4
UniRef50_UPI0000D564D6 Cluster: PREDICTED: similar to Zinc finge... 32 4.2
UniRef50_A7GEP1 Cluster: ABC transporter, permease protein; n=4;... 32 4.2
UniRef50_Q54H00 Cluster: Putative uncharacterized protein; n=1; ... 32 4.2
UniRef50_Q4YV32 Cluster: Putative uncharacterized protein; n=3; ... 32 4.2
UniRef50_Q229Q6 Cluster: Putative uncharacterized protein; n=1; ... 32 4.2
UniRef50_Q98S81 Cluster: Putative uncharacterized protein orf187... 32 5.6
UniRef50_A2DLG0 Cluster: Viral A-type inclusion protein, putativ... 32 5.6
UniRef50_Q5ADS9 Cluster: Putative uncharacterized protein; n=1; ... 32 5.6
UniRef50_Q2GKW8 Cluster: Type IV secretion system VirB6 family p... 31 7.4
UniRef50_Q23HA0 Cluster: Putative uncharacterized protein; n=1; ... 31 7.4
UniRef50_A3GG12 Cluster: Accessory factor to EIF3; n=2; Pichia s... 31 7.4
UniRef50_Q4FRA3 Cluster: K+ uptake transporter, KtrB subunit; n=... 31 9.7
UniRef50_Q1VTD3 Cluster: Putative acidic periplasmic protein; n=... 31 9.7
UniRef50_Q9N2L3 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
UniRef50_A5K2G3 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
UniRef50_A4VE75 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
UniRef50_A2DFY1 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
UniRef50_A0BEU6 Cluster: Chromosome undetermined scaffold_102, w... 31 9.7
UniRef50_A5DUT6 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
>UniRef50_A1IRV2 Cluster: Putative uncharacterized protein; n=2;
Neisseria meningitidis|Rep: Putative uncharacterized
protein - Neisseria meningitidis serogroup A
Length = 431
Score = 35.1 bits (77), Expect = 0.60
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +1
Query: 82 HAFVKRDAEAASTGNAFSEIEKQFVEIKKQVQENFKPDNVKK 207
H F K + A +F ++EK+F E+K+++ + KPD +K
Sbjct: 265 HQFAKAEMGGADFKTSFKQLEKEFYEVKQRLDIDGKPDKEQK 306
>UniRef50_Q1WMS8 Cluster: Putative uncharacterized protein UP10;
n=1; Coprinellus disseminatus|Rep: Putative
uncharacterized protein UP10 - Coprinellus disseminatus
Length = 217
Score = 35.1 bits (77), Expect = 0.60
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = +1
Query: 112 ASTGNAFSEIEKQFVEIKKQVQENFKPDNVKKQFNNMVDDFNKFMSSMNPNSEAPK 279
+ST +E EK+F E++++ E KK + V DFNK + +++ + + PK
Sbjct: 78 SSTSTRKTEAEKRFEEVQRKRLEQRVHKLAKKTHKDRVADFNKHLETLSEHHDIPK 133
>UniRef50_P85216 Cluster: Anionic antimicrobial peptide 2; n=1;
Galleria mellonella|Rep: Anionic antimicrobial peptide 2
- Galleria mellonella (Wax moth)
Length = 60
Score = 34.3 bits (75), Expect = 1.0
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
Frame = +1
Query: 106 EAASTGNAFSEIEKQFVEIKK----QVQENFKPDNVKKQFNNMVDDFNKFMSSMNPNSEA 273
E ST + I++Q E K QVQ F D +K + NN ++ K +++ EA
Sbjct: 1 ETESTPDYLKNIQQQLEEYTKNFNTQVQNAFDSDKIKSEVNNFIESLGKILNT--EKKEA 58
Query: 274 PK 279
PK
Sbjct: 59 PK 60
>UniRef50_A6LW08 Cluster: Signal transduction histidine kinase,
LytS; n=1; Clostridium beijerinckii NCIMB 8052|Rep:
Signal transduction histidine kinase, LytS - Clostridium
beijerinckii NCIMB 8052
Length = 457
Score = 33.5 bits (73), Expect = 1.8
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
Frame = +1
Query: 43 HLIIFLAVCFISVHAFVKRDAEAASTGNAFS-EIEK-QFVEIKKQVQENFKPDNVKKQFN 216
+LI+ LA+C + F++ +E S ++ K QF + + ++ D + K FN
Sbjct: 143 YLILILALCVVYALTFIRHISEGLKGMVEISNKVSKGQFEYYEGNKTDIYELDILTKTFN 202
Query: 217 NMVDDFNKFMSSMN 258
M+ D +SS+N
Sbjct: 203 TMITDIKNLISSLN 216
>UniRef50_A2DE23 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 533
Score = 33.5 bits (73), Expect = 1.8
Identities = 18/75 (24%), Positives = 39/75 (52%), Gaps = 2/75 (2%)
Frame = +1
Query: 73 ISVHAFVKRDAEAASTGNAFSEIEKQFVEIKKQ--VQENFKPDNVKKQFNNMVDDFNKFM 246
I+ A ++++E + ++ + ++KK +Q+NF+ D KK N++ KF+
Sbjct: 54 IAQDAIDQQESEILKKESIIEKLRQMLEQMKKDQILQQNFQSDEAKKLQNDIEQQHKKFL 113
Query: 247 SSMNPNSEAPK*KKT 291
++N ++A KT
Sbjct: 114 ETLNQLNQAKGPAKT 128
>UniRef50_Q5KFM1 Cluster: Putative uncharacterized protein; n=1;
Filobasidiella neoformans|Rep: Putative uncharacterized
protein - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 128
Score = 33.1 bits (72), Expect = 2.4
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = +1
Query: 106 EAASTGNAFSEIEKQFVEIKKQVQENFKPDNVKKQFNNMVDDFNKFMSSMNPNSEAPK 279
E G +E E++F+E +K+ +E KK V +FN + S++ + + P+
Sbjct: 67 ERREDGPKMTEAERRFLETQKRRREERAKHTAKKTHKERVQEFNAKLDSLSEHHDMPR 124
>UniRef50_UPI0000D564D6 Cluster: PREDICTED: similar to Zinc finger
FYVE domain-containing protein 9 (Mothers against
decapentaplegic homolog-interacting protein)
(Madh-interacting protein) (Smad anchor for receptor
activation) (Receptor activation anchor) (hSARA) (Novel
serine protease) (NSP...; n=1; Tribolium castaneum|Rep:
PREDICTED: similar to Zinc finger FYVE domain-containing
protein 9 (Mothers against decapentaplegic
homolog-interacting protein) (Madh-interacting protein)
(Smad anchor for receptor activation) (Receptor
activation anchor) (hSARA) (Novel serine protease)
(NSP... - Tribolium castaneum
Length = 1026
Score = 32.3 bits (70), Expect = 4.2
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +1
Query: 130 FSEIEKQFVEIKKQVQENFKPDNVKKQFNNMVDDFNKFMSS-MNPNSE 270
+SE+ Q KQ NFK NVK NN+ N+++++ ++P E
Sbjct: 17 YSELTDQHSSNHKQNFNNFKNSNVKHSINNVFHSLNEYLNTDISPKCE 64
>UniRef50_A7GEP1 Cluster: ABC transporter, permease protein; n=4;
Clostridium botulinum|Rep: ABC transporter, permease
protein - Clostridium botulinum (strain Langeland / NCTC
10281 / Type F)
Length = 283
Score = 32.3 bits (70), Expect = 4.2
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = -3
Query: 293 SVFFYFGASELGFMLLINLLKSSTMLLNCFLTLSGLKFSCTCFLISTNCFS 141
++F+ LGF+ ++ +L T+ F + +G SC CFL+ + FS
Sbjct: 153 AIFYSLKYYALGFLTVLAILSVFTLFSVIFKSSTGTISSCICFLVGSFIFS 203
>UniRef50_Q54H00 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 922
Score = 32.3 bits (70), Expect = 4.2
Identities = 20/55 (36%), Positives = 26/55 (47%)
Frame = +1
Query: 100 DAEAASTGNAFSEIEKQFVEIKKQVQENFKPDNVKKQFNNMVDDFNKFMSSMNPN 264
D A S NAFS K F +K Q+ +P+N Q NN+ NK + N N
Sbjct: 789 DCLAQSWDNAFSFENKYFDTLKNQINTKNQPNNKVNQINNL----NKVGQNQNQN 839
>UniRef50_Q4YV32 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium berghei
Length = 1298
Score = 32.3 bits (70), Expect = 4.2
Identities = 21/78 (26%), Positives = 37/78 (47%), Gaps = 2/78 (2%)
Frame = +1
Query: 31 KMNKHLIIFLAVCFISVHAFVKRDAEAASTGNAFSEIEKQFVEIKKQVQENFKPDNVKKQ 210
K HLII + I +KR + + + +++ EN K +NVKK
Sbjct: 447 KQRNHLIIVELMSKIQNRLIIKRLNQELTNKVNLENLINDVLKMFSNFVENMKDENVKKF 506
Query: 211 FNNMVDDF--NKFMSSMN 258
+ NM++ + NK +SS++
Sbjct: 507 YMNMINIYFRNKNLSSID 524
>UniRef50_Q229Q6 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 496
Score = 32.3 bits (70), Expect = 4.2
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 4/41 (9%)
Frame = +1
Query: 133 SEIEKQFVEIK----KQVQENFKPDNVKKQFNNMVDDFNKF 243
S I KQ ++++ +Q QEN K +N K N ++DFNK+
Sbjct: 196 SNINKQKLKLESFENEQQQENLKSNNTSKDQQNSLEDFNKY 236
>UniRef50_Q98S81 Cluster: Putative uncharacterized protein orf187;
n=1; Guillardia theta|Rep: Putative uncharacterized
protein orf187 - Guillardia theta (Cryptomonas phi)
Length = 187
Score = 31.9 bits (69), Expect = 5.6
Identities = 19/60 (31%), Positives = 30/60 (50%)
Frame = +1
Query: 88 FVKRDAEAASTGNAFSEIEKQFVEIKKQVQENFKPDNVKKQFNNMVDDFNKFMSSMNPNS 267
FV+ N+ EI+K+++ IK NFK DN+KK N +++F + N S
Sbjct: 37 FVRLSKGLVRDSNSTHEIDKKYIGIKNF---NFKFDNLKKNSNCNINNFISYNIENNEES 93
>UniRef50_A2DLG0 Cluster: Viral A-type inclusion protein, putative;
n=1; Trichomonas vaginalis G3|Rep: Viral A-type inclusion
protein, putative - Trichomonas vaginalis G3
Length = 3369
Score = 31.9 bits (69), Expect = 5.6
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = +1
Query: 94 KRDAEAASTGNAFSEIEKQFVEIKKQVQENFKP-DNVKKQFNNMVDDFNKFMSSMNPNSE 270
K D E ++ E+ K+ E K+QV E N+KK+ N+ N+ + ++ NS+
Sbjct: 1491 KYDEEVSNHNKEVEELTKKDEENKQQVDEKENEISNLKKEIENLKSSLNEKDNEISQNSQ 1550
Query: 271 A 273
A
Sbjct: 1551 A 1551
>UniRef50_Q5ADS9 Cluster: Putative uncharacterized protein; n=1;
Candida albicans|Rep: Putative uncharacterized protein -
Candida albicans (Yeast)
Length = 120
Score = 31.9 bits (69), Expect = 5.6
Identities = 18/45 (40%), Positives = 27/45 (60%)
Frame = -3
Query: 233 KSSTMLLNCFLTLSGLKFSCTCFLISTNCFSISLNALPVLAASAS 99
KS LL+CFLTL L C C LIS++C + ++L ++ A+
Sbjct: 38 KSDCNLLSCFLTL--LSNPC-CLLISSSCLVLETSSLMLVMTGAT 79
>UniRef50_Q2GKW8 Cluster: Type IV secretion system VirB6 family
protein; n=1; Anaplasma phagocytophilum HZ|Rep: Type IV
secretion system VirB6 family protein - Anaplasma
phagocytophilum (strain HZ)
Length = 2360
Score = 31.5 bits (68), Expect = 7.4
Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 4/65 (6%)
Frame = -3
Query: 305 IIIISVFFYFGASELGFMLLINLLKSSTMLLNCF-LTLSGLKFSCTCFLIS---TNCFSI 138
IIII+V FY G GF++ + T L++ + G+ FS L T C S
Sbjct: 436 IIIIAVLFYVGLCLYGFVIYLTAFVGVTFLISLMPILFVGILFSRFKSLFDGWLTQCISF 495
Query: 137 SLNAL 123
S+ A+
Sbjct: 496 SMQAI 500
>UniRef50_Q23HA0 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 644
Score = 31.5 bits (68), Expect = 7.4
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +1
Query: 142 EKQFVEIKKQVQENFKPDNVKKQFNNM 222
+K EIKK E FKPD KKQ N+
Sbjct: 6 QKNLKEIKKGFGEGFKPDKKKKQLKNV 32
>UniRef50_A3GG12 Cluster: Accessory factor to EIF3; n=2; Pichia
stipitis|Rep: Accessory factor to EIF3 - Pichia stipitis
(Yeast)
Length = 1242
Score = 31.5 bits (68), Expect = 7.4
Identities = 24/99 (24%), Positives = 50/99 (50%), Gaps = 4/99 (4%)
Frame = +1
Query: 10 KVVGRDFKMNKHLIIFLAVCFISVHAFVKRDAE-AASTGNAFSEIEKQFVEIK---KQVQ 177
KV+G D + K +++ S+ +VK A+ A ++GN+ +++ Q +K K +
Sbjct: 1147 KVLGPDDALTKQASKYVS----SIETYVKELAQQAVASGNSKIKVKSQTETVKNGKKNGK 1202
Query: 178 ENFKPDNVKKQFNNMVDDFNKFMSSMNPNSEAPK*KKTE 294
++ P + + +DD +F+ NP + K KK++
Sbjct: 1203 KSAIPQPDPEIASKSIDDILQFIEGNNPKKSSKKSKKSK 1241
>UniRef50_Q4FRA3 Cluster: K+ uptake transporter, KtrB subunit; n=4;
Bacteria|Rep: K+ uptake transporter, KtrB subunit -
Psychrobacter arcticum
Length = 586
Score = 31.1 bits (67), Expect = 9.7
Identities = 16/51 (31%), Positives = 30/51 (58%)
Frame = -3
Query: 302 IIISVFFYFGASELGFMLLINLLKSSTMLLNCFLTLSGLKFSCTCFLISTN 150
+IIS+ F FG +GF++++NLL+ + L G +++ +L+S N
Sbjct: 319 LIISITFLFGG--MGFLIVVNLLRYLRYRMQRALCRDGQRYAYQPWLLSIN 367
>UniRef50_Q1VTD3 Cluster: Putative acidic periplasmic protein; n=1;
Psychroflexus torquis ATCC 700755|Rep: Putative acidic
periplasmic protein - Psychroflexus torquis ATCC 700755
Length = 323
Score = 31.1 bits (67), Expect = 9.7
Identities = 23/94 (24%), Positives = 42/94 (44%), Gaps = 10/94 (10%)
Frame = +1
Query: 1 LIRKVVGRDFKMNKHLIIFLAVCFISVHAFVKRDAEAASTGNAFSEI--EKQFVE----- 159
++ + G D K I+F + I+ F + D +A T E+ +K + E
Sbjct: 24 VLNRGYGNDSTHMKKTIVFTILILITQFGFAQNDKKAVKTSKGVLELIKQKDYTEFFNSF 83
Query: 160 ---IKKQVQENFKPDNVKKQFNNMVDDFNKFMSS 252
IK +++E ++ MVDDFN+ +S+
Sbjct: 84 EDTIKMRIKE-YQKSAGSDYIQKMVDDFNETLSN 116
>UniRef50_Q9N2L3 Cluster: Putative uncharacterized protein; n=1;
Caenorhabditis elegans|Rep: Putative uncharacterized
protein - Caenorhabditis elegans
Length = 239
Score = 31.1 bits (67), Expect = 9.7
Identities = 18/71 (25%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Frame = +1
Query: 4 IRKVVGRDFKMNKHLIIFLAVCFISVHAFVKRDAEAASTGNAFSEIEKQFVEIK--KQVQ 177
+ KV + + K ++ A CF+++H D AAS ++ ++ K K++
Sbjct: 42 LEKVSLPNNSLEKLMVKHFANCFVALHTMNSLDELAASAPKFVADFKQAIGNKKTLKELH 101
Query: 178 ENFKPDNVKKQ 210
+NFK + K+Q
Sbjct: 102 DNFKSSDTKQQ 112
>UniRef50_A5K2G3 Cluster: Putative uncharacterized protein; n=1;
Plasmodium vivax|Rep: Putative uncharacterized protein -
Plasmodium vivax
Length = 1544
Score = 31.1 bits (67), Expect = 9.7
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = +1
Query: 181 NFKPDNVKKQFNNMVDDFNKFMSSMNPNSEAPK*KKT 291
NF P KK FNNM NK+++S N + K KKT
Sbjct: 619 NFYPSMKKKPFNNMTFSLNKYLNSY-VNFDRIKNKKT 654
>UniRef50_A4VE75 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 293
Score = 31.1 bits (67), Expect = 9.7
Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Frame = -3
Query: 281 YFGASELGFMLLINLLKSSTM--LLNCFLTLSGLKFSCTCFLISTNCFSISLNAL 123
Y S+ F +LINL +S + LL F +KF T +TN F I N L
Sbjct: 173 YLKQSKRSFYILINLFQSYLICFLLQIFFNFEEIKFCQTNLYKNTNTFDIFFNFL 227
>UniRef50_A2DFY1 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 2553
Score = 31.1 bits (67), Expect = 9.7
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +1
Query: 136 EIEKQFVEIKKQVQENFKP-DNVKKQFNN-MVDDFNKFMSSMNPNSEAPK 279
E + + + K +V F P +N KK F +V FNK M+SMN E P+
Sbjct: 711 EYDDESEKPKMEVDTGFVPVNNEKKSFKPPVVPIFNKLMNSMNNKQEQPE 760
>UniRef50_A0BEU6 Cluster: Chromosome undetermined scaffold_102, whole
genome shotgun sequence; n=4; Paramecium tetraurelia|Rep:
Chromosome undetermined scaffold_102, whole genome
shotgun sequence - Paramecium tetraurelia
Length = 1641
Score = 31.1 bits (67), Expect = 9.7
Identities = 19/87 (21%), Positives = 41/87 (47%), Gaps = 2/87 (2%)
Frame = +1
Query: 4 IRKVVGRDFKMNKHLIIFLAVCFISV-HAFVKRDAEAASTGNAFSEIEKQFVEIKKQVQE 180
+ +++ + F+ +K + CF + ++K G+ I+K+ +EI
Sbjct: 1263 VLEIISQSFQESKGSVDGFKKCFCQLAQKYIKLSMAKCKQGSENGSIKKKVIEI------ 1316
Query: 181 NFKPDNVK-KQFNNMVDDFNKFMSSMN 258
+ N K KQ N ++D+N++ +MN
Sbjct: 1317 GLREGNKKYKQVKNDIEDYNQYKEAMN 1343
>UniRef50_A5DUT6 Cluster: Putative uncharacterized protein; n=1;
Lodderomyces elongisporus NRRL YB-4239|Rep: Putative
uncharacterized protein - Lodderomyces elongisporus
(Yeast) (Saccharomyces elongisporus)
Length = 641
Score = 31.1 bits (67), Expect = 9.7
Identities = 11/44 (25%), Positives = 28/44 (63%)
Frame = -3
Query: 326 VGNSYIGIIIISVFFYFGASELGFMLLINLLKSSTMLLNCFLTL 195
+G ++I +III++ + G + F ++IN++ +++C +T+
Sbjct: 98 LGFNFITLIIIAISHFIGRKAILFAIVINVISLILTIISCIVTI 141
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 265,395,173
Number of Sequences: 1657284
Number of extensions: 3967299
Number of successful extensions: 19109
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 18266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19086
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 19810951153
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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