Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_J16
(650 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein. 106 2e-25
EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein. 106 2e-25
EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein. 92 4e-21
EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein. 92 4e-21
EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein. 91 6e-21
AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein. 91 6e-21
EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage prot... 76 3e-16
AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase prot... 27 0.21
>EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein.
Length = 684
Score = 106 bits (255), Expect = 2e-25
Identities = 56/129 (43%), Positives = 86/129 (66%), Gaps = 1/129 (0%)
Frame = +2
Query: 35 LIALVQSSVVSPKTYHFKTKDVDAVFVERQKKVLSLFQDVDQVNVDD-EYYKIGKDYDVE 211
L+ L+ S+V + Y KT D D F+ +QKKV +L V Q + + +Y G+ +++E
Sbjct: 10 LVGLLAFSLVGAEYYDTKTADKD--FLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIE 67
Query: 212 ANIDNYTNKKAVEEFLKLYRIGYLPKYYEFSIFYQKLREEAIALFHLFYYAKDFETFYKS 391
ANID+YTN AV+EFL +Y+ G LP+ FS++Y +L E ALF LFY+AKDF+ F+K+
Sbjct: 68 ANIDSYTNAAAVKEFLSIYKHGMLPRGELFSLYYPQLLREMSALFKLFYHAKDFDIFFKT 127
Query: 392 AAFARVHLN 418
A +A+ ++N
Sbjct: 128 ALWAKNNIN 136
Score = 39.5 bits (88), Expect = 3e-05
Identities = 19/42 (45%), Positives = 24/42 (57%)
Frame = +3
Query: 420 EGQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLK 545
E Q++Y+ Y AVI R DT P YE+ P FF N + L K
Sbjct: 137 EAQYIYSLYTAVITRPDTKFIQLPPLYEMCPYFFFNSEVLQK 178
>EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein.
Length = 684
Score = 106 bits (255), Expect = 2e-25
Identities = 56/129 (43%), Positives = 86/129 (66%), Gaps = 1/129 (0%)
Frame = +2
Query: 35 LIALVQSSVVSPKTYHFKTKDVDAVFVERQKKVLSLFQDVDQVNVDD-EYYKIGKDYDVE 211
L+ L+ S+V + Y KT D D F+ +QKKV +L V Q + + +Y G+ +++E
Sbjct: 10 LVGLLAFSLVGAEYYDTKTADKD--FLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIE 67
Query: 212 ANIDNYTNKKAVEEFLKLYRIGYLPKYYEFSIFYQKLREEAIALFHLFYYAKDFETFYKS 391
ANID+YTN AV+EFL +Y+ G LP+ FS++Y +L E ALF LFY+AKDF+ F+K+
Sbjct: 68 ANIDSYTNAAAVKEFLSIYKHGMLPRGELFSLYYPQLLREMSALFKLFYHAKDFDIFFKT 127
Query: 392 AAFARVHLN 418
A +A+ ++N
Sbjct: 128 ALWAKNNIN 136
Score = 39.5 bits (88), Expect = 3e-05
Identities = 19/42 (45%), Positives = 24/42 (57%)
Frame = +3
Query: 420 EGQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLK 545
E Q++Y+ Y AVI R DT P YE+ P FF N + L K
Sbjct: 137 EAQYIYSLYTAVITRPDTKFIQLPPLYEMCPYFFFNSEVLQK 178
>EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein.
Length = 686
Score = 92.3 bits (219), Expect = 4e-21
Identities = 53/141 (37%), Positives = 85/141 (60%), Gaps = 2/141 (1%)
Frame = +2
Query: 8 MKTVLVLAGLIALVQSSVVSPKTYHFKTKDVDAVFVERQKKVLSLFQDVDQVNVDD-EYY 184
+ V++L L A+ + S H T D+D F+ +QKK+ L V Q ++ D E+Y
Sbjct: 2 LSKVVLLVALAAICGAQGASYAGRH--TADMD--FLHKQKKIFDLLLYVRQADLSDAEWY 57
Query: 185 KIGKDYDVEANIDNYTNKKAVEEFLKLYRIG-YLPKYYEFSIFYQKLREEAIALFHLFYY 361
+G++YD+E+N+D Y +K V++FL Y+ G +L + F+ + + E LF L Y
Sbjct: 58 DVGRNYDMESNMDMYKDKNVVQKFLWWYKQGMFLSRNAIFTPLNSEQKYEVRMLFELLYN 117
Query: 362 AKDFETFYKSAAFARVHLN*G 424
AKDF+TFYK+AA+AR+ +N G
Sbjct: 118 AKDFQTFYKTAAWARLRMNSG 138
Score = 39.1 bits (87), Expect = 4e-05
Identities = 25/76 (32%), Positives = 36/76 (47%)
Frame = +3
Query: 423 GQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLKIYRTKMPDGILHDAKAINY 602
G F A+ IAV+ R DT PA YE+YP +F + + + KM G ++
Sbjct: 138 GMFTTAFSIAVLYRPDTKYMKFPAIYEIYPNYFFDSSVIEEAQNLKMSRG-----SSVVT 192
Query: 603 GIVQEEEQYVYYANYS 650
G + E Y+ NYS
Sbjct: 193 G-MNNIETYIVNTNYS 207
>EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein.
Length = 686
Score = 92.3 bits (219), Expect = 4e-21
Identities = 53/141 (37%), Positives = 85/141 (60%), Gaps = 2/141 (1%)
Frame = +2
Query: 8 MKTVLVLAGLIALVQSSVVSPKTYHFKTKDVDAVFVERQKKVLSLFQDVDQVNVDD-EYY 184
+ V++L L A+ + S H T D+D F+ +QKK+ L V Q ++ D E+Y
Sbjct: 2 LSKVVLLVALAAICGAQGASYAGRH--TADMD--FLHKQKKIFDLLLYVRQADLSDAEWY 57
Query: 185 KIGKDYDVEANIDNYTNKKAVEEFLKLYRIG-YLPKYYEFSIFYQKLREEAIALFHLFYY 361
+G++YD+E+N+D Y +K V++FL Y+ G +L + F+ + + E LF L Y
Sbjct: 58 DVGRNYDMESNMDMYKDKNVVQKFLWWYKQGMFLSRNAIFTPLNSEQKYEVRMLFELLYN 117
Query: 362 AKDFETFYKSAAFARVHLN*G 424
AKDF+TFYK+AA+AR+ +N G
Sbjct: 118 AKDFQTFYKTAAWARLRMNSG 138
Score = 39.1 bits (87), Expect = 4e-05
Identities = 25/76 (32%), Positives = 36/76 (47%)
Frame = +3
Query: 423 GQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLKIYRTKMPDGILHDAKAINY 602
G F A+ IAV+ R DT PA YE+YP +F + + + KM G ++
Sbjct: 138 GMFTTAFSIAVLYRPDTKYMKFPAIYEIYPNYFFDSSVIEEAQNLKMSRG-----SSVVT 192
Query: 603 GIVQEEEQYVYYANYS 650
G + E Y+ NYS
Sbjct: 193 G-MNNIETYIVNTNYS 207
Score = 21.8 bits (44), Expect = 5.9
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +2
Query: 224 NYTNKKAVEEFLKLYRIGYLPKYYEFSIFYQKLRE 328
NY++K E Y++ Y + E + +Y +RE
Sbjct: 205 NYSSKNMREYNDPEYKLDYFMEDVELNAYYYYMRE 239
>EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein.
Length = 683
Score = 91.5 bits (217), Expect = 6e-21
Identities = 43/110 (39%), Positives = 69/110 (62%), Gaps = 1/110 (0%)
Frame = +2
Query: 92 KDVDAVFVERQKKVLSLFQDVDQVNV-DDEYYKIGKDYDVEANIDNYTNKKAVEEFLKLY 268
K D +V RQK + LF VDQ V E Y+ + +++ N+DNY +K+AV EF++L
Sbjct: 25 KVADKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLL 84
Query: 269 RIGYLPKYYEFSIFYQKLREEAIALFHLFYYAKDFETFYKSAAFARVHLN 418
+ G LP+ F++ +++R +A+ LF L Y AK F+ FY +A +AR ++N
Sbjct: 85 KHGMLPRGQVFTMMNKEMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVN 134
Score = 39.1 bits (87), Expect = 4e-05
Identities = 20/50 (40%), Positives = 25/50 (50%)
Frame = +3
Query: 420 EGQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLKIYRTKMPD 569
E +LYA +AVI R DT P YEV P + N + + K Y M D
Sbjct: 135 EQMYLYALSVAVIHRPDTKLMKLPPMYEVMPHLYFNDEVMQKAYNIAMGD 184
>AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein.
Length = 683
Score = 91.5 bits (217), Expect = 6e-21
Identities = 43/110 (39%), Positives = 69/110 (62%), Gaps = 1/110 (0%)
Frame = +2
Query: 92 KDVDAVFVERQKKVLSLFQDVDQVNV-DDEYYKIGKDYDVEANIDNYTNKKAVEEFLKLY 268
K D +V RQK + LF VDQ V E Y+ + +++ N+DNY +K+AV EF++L
Sbjct: 25 KVADKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLL 84
Query: 269 RIGYLPKYYEFSIFYQKLREEAIALFHLFYYAKDFETFYKSAAFARVHLN 418
+ G LP+ F++ +++R +A+ LF L Y AK F+ FY +A +AR ++N
Sbjct: 85 KHGMLPRGQVFTMMNKEMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVN 134
Score = 39.1 bits (87), Expect = 4e-05
Identities = 20/50 (40%), Positives = 25/50 (50%)
Frame = +3
Query: 420 EGQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVNMDTLLKIYRTKMPD 569
E +LYA +AVI R DT P YEV P + N + + K Y M D
Sbjct: 135 EQMYLYALSVAVIHRPDTKLMKLPPMYEVMPHLYFNDEVMQKAYNIAMGD 184
>EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage protein
protein.
Length = 1010
Score = 75.8 bits (178), Expect = 3e-16
Identities = 45/134 (33%), Positives = 70/134 (52%), Gaps = 1/134 (0%)
Frame = +2
Query: 35 LIALVQSSVVSPKTYHFKTKDVDAVFVERQKKVLSLFQDVDQVNVDDEYYKIGKDYDVEA 214
L+ALV V +P K + D + +Q+ V+ L Q + Q + E +G YD+E+
Sbjct: 7 LLALVALGVCAPNV---KQRAADQDLLNKQQDVIQLLQKISQPIPNQELQNLGASYDIES 63
Query: 215 NIDNYTNKKAVEEFLKLYRIGYL-PKYYEFSIFYQKLREEAIALFHLFYYAKDFETFYKS 391
N Y N V + + G + P+ FS +LR+E L+ + AKD++TF K+
Sbjct: 64 NSHQYKNPIIVMYYAGAVKAGLVQPQGTTFSNSISQLRKEVSLLYRILLGAKDYQTFLKT 123
Query: 392 AAFARVHLN*GTVL 433
AA+ARVH+N G L
Sbjct: 124 AAWARVHVNEGQFL 137
Score = 37.1 bits (82), Expect = 1e-04
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 420 EGQFLYAYYIAVIQRNDTHGFV*PAPYEVYPQ 515
EGQFL A+ AV+ R DT + P YE+ PQ
Sbjct: 133 EGQFLKAFVAAVLTRQDTQSVIFPPVYEILPQ 164
Score = 22.6 bits (46), Expect = 3.4
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 101 DAVFVERQKKVLSLFQDVDQ 160
D VF + KKV++L+Q Q
Sbjct: 431 DPVFYQLYKKVMNLYQQYQQ 450
>AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase
protein.
Length = 693
Score = 26.6 bits (56), Expect = 0.21
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +3
Query: 429 FLYAYYIAVIQRNDTHGFV*PAPYEVYPQFFVN 527
F+YA +A++ R DT P EV+P +++
Sbjct: 125 FIYALSVAILHRPDTKDLPVPPLTEVFPDKYMD 157
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 181,882
Number of Sequences: 438
Number of extensions: 3802
Number of successful extensions: 27
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19682733
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -