Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_J12
(280 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B) cont... 124 7e-30
At4g25740.1 68417.m03706 40S ribosomal protein S10 (RPS10A) 40S ... 120 2e-28
At5g52650.1 68418.m06536 40S ribosomal protein S10 (RPS10C) cont... 116 2e-27
At5g36210.1 68418.m04365 expressed protein 28 1.1
At1g16370.1 68414.m01958 transporter-related low similarity to o... 27 1.9
At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 27 2.4
At2g18870.1 68415.m02200 hypothetical protein contains 1 transme... 27 2.4
At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT... 26 3.2
At5g59500.1 68418.m07457 expressed protein 25 5.7
At2g22730.1 68415.m02694 transporter-related low similarity to s... 25 5.7
At2g26650.1 68415.m03197 potassium channel protein 1 (AKT1) iden... 25 7.5
At1g01010.1 68414.m00001 no apical meristem (NAM) family protein... 25 7.5
At5g19670.1 68418.m02340 exostosin family protein contains Pfam ... 25 9.9
At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,... 25 9.9
At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,... 25 9.9
At3g45870.1 68416.m04964 integral membrane family protein / nodu... 25 9.9
At1g54550.1 68414.m06221 F-box family protein contains Pfam:PF00... 25 9.9
At1g48310.1 68414.m05396 SNF2 domain-containing protein / helica... 25 9.9
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S... 25 9.9
>At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B)
contains similarity to 40S ribosomal protein S10
Length = 180
Score = 124 bits (300), Expect = 7e-30
Identities = 52/86 (60%), Positives = 70/86 (81%)
Frame = +1
Query: 22 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 201
M++ + NR I +YLFKEGV+ AKKD++ P+HP +E +PNLQVIK MQS KS+ YV+E F
Sbjct: 1 MIISETNRREISKYLFKEGVLFAKKDFNLPQHPLIESVPNLQVIKLMQSFKSKEYVRETF 60
Query: 202 AWRHFYWYLTNEGIEYLRIFLHLPPE 279
AW H+YW+LTNEGI++LR +L+LP E
Sbjct: 61 AWMHYYWFLTNEGIDFLRTYLNLPSE 86
>At4g25740.1 68417.m03706 40S ribosomal protein S10 (RPS10A) 40S
ribosomal protein S10 - Lumbricus rubellus, PID:e1329701
Length = 177
Score = 120 bits (288), Expect = 2e-28
Identities = 52/86 (60%), Positives = 69/86 (80%)
Frame = +1
Query: 22 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 201
M++ + NR I +YLFKEGV AKKD++ PKHP ++ +PNLQVIK MQS KS+ YV+E F
Sbjct: 1 MIISENNRREICKYLFKEGVCFAKKDFNLPKHPLID-VPNLQVIKLMQSFKSKEYVRETF 59
Query: 202 AWRHFYWYLTNEGIEYLRIFLHLPPE 279
AW H+YW+LTNEGIE+LR +L+LP +
Sbjct: 60 AWMHYYWFLTNEGIEFLRTYLNLPSD 85
>At5g52650.1 68418.m06536 40S ribosomal protein S10 (RPS10C)
contains similarity to 40S ribosomal protein S10
Length = 179
Score = 116 bits (280), Expect = 2e-27
Identities = 51/86 (59%), Positives = 68/86 (79%)
Frame = +1
Query: 22 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 201
M++ + NR I +YLFKEGV AKKD++ KHP ++ +PNLQVIK MQS KS+ YV+E F
Sbjct: 1 MIISEANRKEICKYLFKEGVCFAKKDFNLAKHPLID-VPNLQVIKLMQSFKSKEYVRETF 59
Query: 202 AWRHFYWYLTNEGIEYLRIFLHLPPE 279
AW H+YW+LTNEGIE+LR +L+LP +
Sbjct: 60 AWMHYYWFLTNEGIEFLRTYLNLPSD 85
>At5g36210.1 68418.m04365 expressed protein
Length = 676
Score = 27.9 bits (59), Expect = 1.1
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +1
Query: 19 KMLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKH 117
K++ P Q+R IYE L K+G+ VA +Y +H
Sbjct: 603 KVVTPDQSR-KIYEALKKKGLPVALVEYEGEQH 634
>At1g16370.1 68414.m01958 transporter-related low similarity to
organic cation transporter OCTN1 from [Homo sapiens]
GI:2605501, [Mus musculus] GI:4126605, [Rattus
norvegicus] GI:5679326; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 521
Score = 27.1 bits (57), Expect = 1.9
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +1
Query: 118 PDLEKIPNLQVIKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIEYLRIFLHL 270
P IP + SL ++ + +WR+ Y Y + + Y IFL+L
Sbjct: 210 PRATMIPFTLFVLGFMSLSGIAFLAQDSSWRYLYLYTSVPAVFYC-IFLYL 259
>At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative
similar to mitogen-activated protein kinase [Arabidopsis
thaliana] gi|1255448|dbj|BAA09057; contains Pfam
PF00069: Protein kinase domain
Length = 560
Score = 26.6 bits (56), Expect = 2.4
Identities = 23/91 (25%), Positives = 42/91 (46%), Gaps = 1/91 (1%)
Frame = +1
Query: 1 VESWLSKMLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSR 180
+ SWL L+ + + S+YE + ++G A K+ L+K +Q + +Q L+
Sbjct: 300 ITSWLKGQLLGRGSYASVYEAISEDGDFFAVKEVSL-----LDK--GIQAQECIQQLEGE 352
Query: 181 GYVKEQFAWRHFYWYL-TNEGIEYLRIFLHL 270
+ Q ++ Y T + + L IFL L
Sbjct: 353 IALLSQLQHQNIVRYRGTAKDVSKLYIFLEL 383
>At2g18870.1 68415.m02200 hypothetical protein contains 1
transmembrane domain; tandem duplication of fibronectin
type III domain protein (GI:3004551)
(TIGR_Ath1:At2g18880) [Arabidopsis thaliana]
Length = 239
Score = 26.6 bits (56), Expect = 2.4
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +1
Query: 94 KDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQFAWRHFYWY 225
K A K+P+ + Q +K ++ L+ GYV+ F + W+
Sbjct: 129 KPEKAKKNPESYGLGLEQCVKIIRKLECSGYVESTFRQKFLTWF 172
>At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT3)
identical to potassium channel [Arabidopsis thaliana]
gi|1100898|gb|AAA97865; Note: also identical to AKT3
[Arabidopsis thaliana] gi|1172218|gb|AAA96153, which is
a truncated version of AKT2, PMID:10852932; member of
the 1 pore, 6 transmembrane (1P/6TM- Shaker-type) K+
channel family, PMID:11500563; identical to cDNA
inward-rectifying K+ channel (AKT3) GI:1172219
Length = 802
Score = 26.2 bits (55), Expect = 3.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 34 KQNRVSIYEYLFKEGVMVAKKDYH 105
KQN V + + L K+G+ V +D+H
Sbjct: 647 KQNNVEVMKALLKQGLNVDTEDHH 670
>At5g59500.1 68418.m07457 expressed protein
Length = 396
Score = 25.4 bits (53), Expect = 5.7
Identities = 16/52 (30%), Positives = 23/52 (44%)
Frame = +1
Query: 67 FKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQFAWRHFYW 222
F G MV K K D+ K+ L I + + K+ V + F W +F W
Sbjct: 97 FSFGSMVKKVRELKVKPMDVVKLTLLLSILTVAAKKTLTLVLDPFFWMYFSW 148
>At2g22730.1 68415.m02694 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 510
Score = 25.4 bits (53), Expect = 5.7
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +1
Query: 181 GYVKEQFAWRHFYW 222
GYV + F+WR+ +W
Sbjct: 207 GYVGKHFSWRYAFW 220
>At2g26650.1 68415.m03197 potassium channel protein 1 (AKT1)
identical to AKT1 [Arabidopsis thaliana]
gi|563112|gb|AAA96810; member of the 1 pore, 6
transmembrane (1P/6TM- Shaker-type) K+ channel family,
PMID:11500563
Length = 857
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +1
Query: 37 QNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAM 162
+ + + +YL ++G V K+D H DL + + IKA+
Sbjct: 658 EENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKAL 699
>At1g01010.1 68414.m00001 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAC domain protein NAM GB: AAD17313
GI:4325282 from [Arabidopsis thaliana]
Length = 429
Score = 25.0 bits (52), Expect = 7.5
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +1
Query: 109 PKHPDLEKIPNLQVIKAMQSLKSRGYVKEQ 198
P H ++ IP+L +I+ + + K++ K+Q
Sbjct: 333 PGHTRIDDIPSLNIIEPLHNYKAQEQPKQQ 362
>At5g19670.1 68418.m02340 exostosin family protein contains Pfam
domain, PF03016: Exostosin family
Length = 600
Score = 24.6 bits (51), Expect = 9.9
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Frame = +1
Query: 133 IPNLQVIKAMQSLKSRGYVKEQFA----WRHFYWYLTNEGIEYLRIFLH 267
IP L+ I + S+ YVK Q A RHF W+ E + + LH
Sbjct: 541 IPRLKDI--LLSIPEDKYVKMQMAVRKAQRHFLWHAKPEKYDLFHMVLH 587
>At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 788
Score = 24.6 bits (51), Expect = 9.9
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = +1
Query: 49 SIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQFAWR 210
SI E + E +K + + DLEKIP L+V + + K G + ++ WR
Sbjct: 438 SIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFVHFQK--GITEPRWGWR 489
>At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 558
Score = 24.6 bits (51), Expect = 9.9
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = +1
Query: 49 SIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQFAWR 210
SI E + E +K + + DLEKIP L+V + + K G + ++ WR
Sbjct: 442 SIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFVHFQK--GITEPRWGWR 493
>At3g45870.1 68416.m04964 integral membrane family protein / nodulin
MtN21-related simlar to MtN21 GI:2598575 (root nodule
development) Medicago truncatula, EMBL:MTY15293
Length = 385
Score = 24.6 bits (51), Expect = 9.9
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = -3
Query: 146 WRLGIFSRSGCLGAW*SFLATMTPSLKRY 60
W LG+ G +FLA P LK+Y
Sbjct: 194 WNLGVLCLIGNCTCMAAFLAIQAPVLKKY 222
>At1g54550.1 68414.m06221 F-box family protein contains Pfam:PF00646
F-box domain; contains TIGRFAM TIGR01640 : F-box protein
interaction domain
Length = 362
Score = 24.6 bits (51), Expect = 9.9
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = -2
Query: 87 YH-DSLLEEVLIDRNTVLFWHQHLGQ 13
YH D LL + D ++V+ W+ +LGQ
Sbjct: 102 YHCDGLLLCIAKDNSSVMVWNPYLGQ 127
>At1g48310.1 68414.m05396 SNF2 domain-containing protein / helicase
domain-containing protein contains similarity to
DNA-dependent ATPase A GI:6651385 from [Bos taurus]};
contains PFam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2
Length = 673
Score = 24.6 bits (51), Expect = 9.9
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 31 PKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIP 138
P++ +VS+ L GV+ AK Y+ + KIP
Sbjct: 67 PQEQKVSVKILLHSSGVLAAKFPYNQVVVDAVRKIP 102
>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
Similar to gb|U04299 mannosyl-oligosaccharide
alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
and gb|AA394707 come from this gene
Length = 574
Score = 24.6 bits (51), Expect = 9.9
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 49 SIYEYLFKEGVMVAKKDY 102
S YEYL K ++ K+DY
Sbjct: 275 SFYEYLLKAYILFGKEDY 292
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,142,541
Number of Sequences: 28952
Number of extensions: 109063
Number of successful extensions: 289
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 287
length of database: 12,070,560
effective HSP length: 69
effective length of database: 10,072,872
effective search space used: 231676056
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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