Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_I23
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 149 2e-36
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 147 5e-36
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 145 3e-35
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 145 3e-35
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 91 8e-19
At4g01810.1 68417.m00238 protein transport protein-related relat... 29 3.6
At2g37660.1 68415.m04619 expressed protein 28 4.7
At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containi... 28 6.2
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 149 bits (360), Expect = 2e-36
Identities = 69/122 (56%), Positives = 84/122 (68%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF AG++ G EIWRI+NFE V V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 378 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGS 557
G +TSQDEAG AA+ TV LD GG AVQHRE GHES+ FL YF + L+GG SG
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 558 TT 563
T
Sbjct: 128 KT 129
Score = 51.2 bits (117), Expect = 6e-07
Identities = 33/133 (24%), Positives = 54/133 (40%)
Frame = +3
Query: 168 KDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRN 347
K A + P + G+ +E+W + + +D GK Y GD Y++L T
Sbjct: 374 KSAPVNEDIPPLLESGGK---LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGER 430
Query: 348 NLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQ 527
+ + W G ++ ++ A L + + G VQ R G E F+ F +
Sbjct: 431 KDEYFLSCWFGKKSIPEDQDTAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALF-QPMV 489
Query: 528 YLDGGNPSGSTTS 566
L GG SG +S
Sbjct: 490 VLKGGLSSGYKSS 502
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 147 bits (357), Expect = 5e-36
Identities = 67/119 (56%), Positives = 83/119 (69%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF G++ G EIWRI+NFEPV V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 378 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSG 554
G +TSQDEAG AA+ TV LD GG AVQ+RE GHES+ FL YF + L+GG SG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASG 128
Score = 49.6 bits (113), Expect = 2e-06
Identities = 38/148 (25%), Positives = 58/148 (39%)
Frame = +3
Query: 123 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 302
++QQ V + LS K + P G+ +E+W I + GK Y
Sbjct: 364 LKQQGVG--LKGLS-KSTPVNEDIPPLLEGGGK---LEVWYIDANSKTVLSKDHVGKLYS 417
Query: 303 GDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMG 482
GD Y++L T + + W G ++Q++ A L + + G VQ R G
Sbjct: 418 GDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLASTMTNSLKGRPVQARIFEG 477
Query: 483 HESNHFLQYFTSALQYLDGGNPSGSTTS 566
E F+ F + L GG SG S
Sbjct: 478 KEPPQFVALF-QHMVVLKGGLSSGYKNS 504
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 145 bits (351), Expect = 3e-35
Identities = 63/118 (53%), Positives = 84/118 (71%)
Frame = +3
Query: 201 AFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIG 380
A AG+++G+EIWRI+NF+PV V + GKF+ GDSYI+LKTT+ + +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 381 SETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSG 554
++SQDEAGA A++TV LD GG AVQ+RE GHE+ FL YF + +GG SG
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASG 128
Score = 40.3 bits (90), Expect = 0.001
Identities = 31/141 (21%), Positives = 60/141 (42%), Gaps = 2/141 (1%)
Frame = +3
Query: 123 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 302
+++Q VN Q + + +K P G +++WRI E + +++ + KFY
Sbjct: 364 LQRQGVNVQ-GLVKTSSSSSKDEPKPYIDGTGN---LQVWRINCEEKILLEAAEQSKFYS 419
Query: 303 GDSYIILKT--TSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREA 476
GD YI+ + D+ +L + W G ++ +++ +A L + + Q R
Sbjct: 420 GDCYILQYSYPGEDREEHL---VGTWFGKQSVEEDRASAISLANKMVESMKFVPAQARIN 476
Query: 477 MGHESNHFLQYFTSALQYLDG 539
G E F S + + G
Sbjct: 477 EGKEPIQFFVIMQSFITFKGG 497
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 145 bits (351), Expect = 3e-35
Identities = 66/119 (55%), Positives = 81/119 (68%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF AG++AG+EIWRI+NF P + GKF+ GDSYI+LKTT+ K L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 378 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSG 554
G +TSQDEAG AA+ TV LD GG AVQ+RE GHE+ FL YF + +GG SG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASG 128
Score = 41.5 bits (93), Expect = 5e-04
Identities = 26/108 (24%), Positives = 45/108 (41%)
Frame = +3
Query: 231 VEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGA 410
+++WR+ +Q+ D KFY GD Y+ + + I W G ++ ++E G+
Sbjct: 394 LQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKE-EVLIGTWFGKQSVEEERGS 452
Query: 411 AAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSG 554
A + + + Q R G E F S + + GG SG
Sbjct: 453 AVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSG 499
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 90.6 bits (215), Expect = 8e-19
Identities = 39/122 (31%), Positives = 70/122 (57%)
Frame = +3
Query: 174 ARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNL 353
+R + AF G ++G+EIW + N + +++ FGKF+ G++Y++L+T K +
Sbjct: 2 SRLSKDIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESP 61
Query: 354 SWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYL 533
+DIHYW+G + ++ ++ A+ + LD G VQ+RE G E+ FL YF + +
Sbjct: 62 QYDIHYWLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPV 121
Query: 534 DG 539
+G
Sbjct: 122 EG 123
Score = 35.9 bits (79), Expect = 0.024
Identities = 26/115 (22%), Positives = 48/115 (41%), Gaps = 1/115 (0%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYII-LKTTSDKRNNLSWDIHYW 374
P + N + +++WR+ + + D K + GD Y++ K T +R ++ W
Sbjct: 382 PLYTNC--RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL--LYVW 437
Query: 375 IGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDG 539
IG E+ Q + A + G +V G+E + F F S + + G
Sbjct: 438 IGCESIQQDRADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVFKGG 492
>At4g01810.1 68417.m00238 protein transport protein-related related
to Sec23 protein [Homo sapiens] gi|1296664|emb|CAA65774
Length = 880
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +3
Query: 261 PVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSW--DIHYWIGSETSQDEAGAAAIL 422
P + ++ G F + +Y L SDK L D+ W+G+E S DE +AA+L
Sbjct: 733 PRCLMHQEGGTFEELPAYD-LSMQSDKAVILDHGTDVFIWLGAELSADEVKSAAVL 787
>At2g37660.1 68415.m04619 expressed protein
Length = 325
Score = 28.3 bits (60), Expect = 4.7
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +3
Query: 372 WIGSETSQDEAGAAAILTVGLDDKFGGAAVQH-REAMGHESNHFLQYFTSALQYL-DGGN 545
WIG + D A AA + + L GG + H ++G+ + L + A QYL D G
Sbjct: 182 WIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSIGNA--NILVWKRKAEQYLADSGI 239
Query: 546 P 548
P
Sbjct: 240 P 240
>At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containing
protein low similiarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile: PF01535 PPR
repeat
Length = 409
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = -2
Query: 333 MSSLELYRNLLCRTSQSLSIGLRLVQNSESAKF 235
M++ L+ +L CR + + +G ++N+ES++F
Sbjct: 1 MATRSLFHSLRCRLTNNGVLGSNFIRNAESSRF 33
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,549,432
Number of Sequences: 28952
Number of extensions: 339345
Number of successful extensions: 894
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 893
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -