Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_I04
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11130.1 68416.m01349 clathrin heavy chain, putative similar ... 270 4e-73
At3g08530.1 68416.m00990 clathrin heavy chain, putative similar ... 268 1e-72
At4g17140.1 68417.m02580 pleckstrin homology (PH) domain-contain... 29 1.7
At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam ... 29 2.2
At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam ... 29 2.2
At1g42440.1 68414.m04894 expressed protein contains Pfam domain,... 28 3.9
At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low sim... 28 5.1
At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE... 27 6.8
At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containi... 27 6.8
At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containi... 27 6.8
At4g28810.1 68417.m04119 expressed protein PMID: 12679534, putat... 27 6.8
At1g60987.1 68414.m06866 expressed protein 27 6.8
At1g42430.1 68414.m04893 expressed protein 27 6.8
At3g59140.1 68416.m06593 ABC transporter family protein putative... 27 9.0
>At3g11130.1 68416.m01349 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1705
Score = 270 bits (662), Expect = 4e-73
Identities = 117/191 (61%), Positives = 158/191 (82%)
Frame = +3
Query: 3 KLLYNNVSNFARLAITLVHLKEFQGAVDSARKANSTRTWKEVCFACVDAGEFRLAQMCGL 182
K++Y +SN+A+LA+TLV L++FQGAVD+ARKANS +TWKEVCFACVDA EFRLAQ+CGL
Sbjct: 1229 KIIYAFISNWAKLAVTLVKLQQFQGAVDAARKANSAKTWKEVCFACVDAEEFRLAQICGL 1288
Query: 183 HIVVHADELEDLINYYQDRGHFDELISLLEAALGLERAHMGMFTELAILYSKYKPAKMRE 362
+I++ D+LE++ YYQ+RG F+ELISL+E+ LGLERAHMG+FTEL +LY++Y+ K+ E
Sbjct: 1289 NIIIQVDDLEEVSEYYQNRGCFNELISLMESGLGLERAHMGIFTELGVLYARYRYEKLME 1348
Query: 363 HLELFWSRVNIPKVLRAAEHAHLWSELVFLYDKYEEYDNAALTMMQHPTEAWREGHFKDI 542
H++LF +R+NIPK++RA + W EL +LY +Y+E+DNAA T+M H EAW FKDI
Sbjct: 1349 HIKLFSTRLNIPKLIRACDEQQHWQELTYLYIQYDEFDNAATTVMNHSPEAWEHMQFKDI 1408
Query: 543 ITKVANMELYY 575
+ KVAN+ELYY
Sbjct: 1409 VAKVANVELYY 1419
>At3g08530.1 68416.m00990 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1703
Score = 268 bits (658), Expect = 1e-72
Identities = 116/191 (60%), Positives = 157/191 (82%)
Frame = +3
Query: 3 KLLYNNVSNFARLAITLVHLKEFQGAVDSARKANSTRTWKEVCFACVDAGEFRLAQMCGL 182
K++Y +SN+ +LA+TLV L++FQGAVD+ARKANS +TWKEVCFACVDA EFRLAQ+CGL
Sbjct: 1229 KIIYAFISNWGKLAVTLVKLQQFQGAVDAARKANSAKTWKEVCFACVDAEEFRLAQICGL 1288
Query: 183 HIVVHADELEDLINYYQDRGHFDELISLLEAALGLERAHMGMFTELAILYSKYKPAKMRE 362
+I++ D+LE++ YYQ+RG F+ELISL+E+ LGLERAHMG+FTEL +LY++Y+ K+ E
Sbjct: 1289 NIIIQVDDLEEVSEYYQNRGCFNELISLMESGLGLERAHMGIFTELGVLYARYRYEKLME 1348
Query: 363 HLELFWSRVNIPKVLRAAEHAHLWSELVFLYDKYEEYDNAALTMMQHPTEAWREGHFKDI 542
H++LF +R+NIPK++RA + W EL +LY +Y+E+DNAA T+M H EAW FKDI
Sbjct: 1349 HIKLFSTRLNIPKLIRACDEQQHWQELTYLYIQYDEFDNAATTVMNHSPEAWEHMQFKDI 1408
Query: 543 ITKVANMELYY 575
+ KVAN+ELYY
Sbjct: 1409 VAKVANVELYY 1419
>At4g17140.1 68417.m02580 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 1322
Score = 29.5 bits (63), Expect = 1.7
Identities = 17/79 (21%), Positives = 37/79 (46%)
Frame = +3
Query: 105 STRTWKEVCFACVDAGEFRLAQMCGLHIVVHADELEDLINYYQDRGHFDELISLLEAALG 284
S + + E A + G +RL+ GL D ++ ++N+++ + I+ LE A
Sbjct: 471 SVKLFPETKIADIKLGRYRLSSPSGLLAEYLKDSIDGIVNFFESSTAVSQTIA-LETAAA 529
Query: 285 LERAHMGMFTELAILYSKY 341
++ + TE +Y ++
Sbjct: 530 VQDDYKHELTEEMDMYLQF 548
>At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 299
Score = 29.1 bits (62), Expect = 2.2
Identities = 27/99 (27%), Positives = 40/99 (40%)
Frame = -3
Query: 522 RAKPRSDAASW*APRCHTPRTYHTGTLIPTTGVRVPLPVEPSEC*HATRTAPSVPSS*PA 343
++KP S AAS + ++ T P+ + P V TR P P+ PA
Sbjct: 76 KSKPASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPAPTRPPPPAPTPTPA 135
Query: 342 CT*SRVSPAL*TCPCELVPGPMPLPIN*SVHQSVPDPDN 226
P T E V P+P P+ ++ S P PD+
Sbjct: 136 -------PVAAT---ETVTTPIPEPVPATISSSTPAPDS 164
>At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 419
Score = 29.1 bits (62), Expect = 2.2
Identities = 27/99 (27%), Positives = 40/99 (40%)
Frame = -3
Query: 522 RAKPRSDAASW*APRCHTPRTYHTGTLIPTTGVRVPLPVEPSEC*HATRTAPSVPSS*PA 343
++KP S AAS + ++ T P+ + P V TR P P+ PA
Sbjct: 76 KSKPASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPAPTRPPPPAPTPTPA 135
Query: 342 CT*SRVSPAL*TCPCELVPGPMPLPIN*SVHQSVPDPDN 226
P T E V P+P P+ ++ S P PD+
Sbjct: 136 -------PVAAT---ETVTTPIPEPVPATISSSTPAPDS 164
>At1g42440.1 68414.m04894 expressed protein contains Pfam domain,
PF04950: Protein of unknown function (DUF663)
Length = 793
Score = 28.3 bits (60), Expect = 3.9
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -2
Query: 406 RTFGMLTRDQNSSKCSLILAG 344
R GML D++S KC+L+L+G
Sbjct: 256 RKAGMLVDDESSGKCTLLLSG 276
>At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low
similarity to breast cancer susceptibility protein
[Gallus gallus] GI:19568157; contains Pfam profile
PF00634: BRCA2 repeat
Length = 1155
Score = 27.9 bits (59), Expect = 5.1
Identities = 9/27 (33%), Positives = 17/27 (62%)
Frame = +3
Query: 495 MQHPTEAWREGHFKDIITKVANMELYY 575
+QH + W H++ I+ K+A ++YY
Sbjct: 570 LQHASRKWVTNHYRWIVWKLACYDIYY 596
>At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE) /
sigma-like factor (SIG5) identical to RNA polymerase
sigma subunit SigE [Arabidopsis thaliana] GI:4972299,
sigma-like factor [Arabidopsis thaliana] GI:4033838;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2
Length = 517
Score = 27.5 bits (58), Expect = 6.8
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +3
Query: 366 LELFWSRVNIPKVLRAAEHAHLWSELVFLYDKYEEYDNAALTMMQHPTEA 515
+ L W ++ IP VL + EHA L+ + + E D ++ + P EA
Sbjct: 207 VSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREA 256
>At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 939
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/44 (31%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = -2
Query: 130 QTSFHVRVELAFLALST-APWNSLRCTSVMANRAKLLTLLYNSL 2
+T+ ++ + ++ ST +PWN++ C S AKL LY+ L
Sbjct: 744 ETALNIFHQTKNISSSTISPWNAIICGSATHGHAKLALDLYSDL 787
>At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 938
Score = 27.5 bits (58), Expect = 6.8
Identities = 19/78 (24%), Positives = 33/78 (42%)
Frame = +3
Query: 141 VDAGEFRLAQMCGLHIVVHADELEDLINYYQDRGHFDELISLLEAALGLERAHMGMFTEL 320
+ + EF + QM ++ + +IN Y +G +E +SLL G FT
Sbjct: 380 LSSAEFIITQMLEKSVIPNVVTYSSMINGYVKKGMLEEAVSLLRKMEDQNVVPNG-FTYG 438
Query: 321 AILYSKYKPAKMREHLEL 374
++ +K K +EL
Sbjct: 439 TVIDGLFKAGKEEMAIEL 456
>At4g28810.1 68417.m04119 expressed protein PMID: 12679534, putative
bHLH127 transcription factor
Length = 880
Score = 27.5 bits (58), Expect = 6.8
Identities = 13/53 (24%), Positives = 28/53 (52%)
Frame = -1
Query: 161 SELARVDASEANLLPCPSRVSFPCAVDGTLEFLEVHKRNGQPCKVADIVVQQF 3
S+L+RV + P P++ FP ++ +F+ +H+ QP +++ Q+
Sbjct: 519 SDLSRVHVPSLHSNPVPNQPRFPAYINPYSQFVGLHQMQ-QPPLPLQVILSQY 570
>At1g60987.1 68414.m06866 expressed protein
Length = 97
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Frame = -1
Query: 176 THLSQSELARVDASEANLLPCPSRVSF---PCAVDGTLEFLEV 57
+HLSQ E +R++ + CPS++ C VDG + L++
Sbjct: 19 SHLSQGEESRMNINAERRPWCPSKIQMFDTNCEVDGAKQCLDL 61
>At1g42430.1 68414.m04893 expressed protein
Length = 435
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/51 (27%), Positives = 18/51 (35%)
Frame = +1
Query: 268 WKRHWAWNELTWACSQSWRYSTLSTSRLR*GNTWSCSGRVSTFRRFYGQRN 420
W WA EL W S R G TW S + R +G+ +
Sbjct: 296 WTDKWAETELGTKWGDKWEEKFFSGIGSRQGETWHVSPNSDRWSRTWGEEH 346
>At3g59140.1 68416.m06593 ABC transporter family protein putative
multi resistance protein mrp - Arabidopsis thaliana,
EMBL:ATMRPPROT
Length = 1453
Score = 27.1 bits (57), Expect = 9.0
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 435 SELVFLYDKYEEYDNAALTMMQH-PTEAWREGHFKDIITKVANMEL 569
SEL+ D+ + N +L M+ AW E HFK +I K+ N+EL
Sbjct: 452 SELMTSQDERLKACNESLVNMKVLKLYAW-ESHFKKVIEKLRNIEL 496
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,202,355
Number of Sequences: 28952
Number of extensions: 246358
Number of successful extensions: 756
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 724
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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