BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_H23 (590 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 68 4e-12 At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 62 4e-10 At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 55 3e-08 At1g65520.1 68414.m07434 enoyl-CoA hydratase/isomerase family pr... 55 4e-08 At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar t... 52 2e-07 At4g14440.1 68417.m02227 enoyl-CoA hydratase/isomerase family pr... 52 4e-07 At4g14430.1 68417.m02226 enoyl-CoA hydratase/isomerase family pr... 44 6e-05 At1g02790.1 68414.m00235 exopolygalacturonase / galacturan 1,4-a... 36 0.015 At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydro... 34 0.062 At4g32910.1 68417.m04683 expressed protein 31 0.76 At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family pr... 31 0.76 At2g36450.1 68415.m04474 AP2 domain-containing protein low simil... 29 3.1 At1g66600.1 68414.m07568 WRKY family transcription factor contai... 28 4.1 At2g42570.1 68415.m05268 expressed protein 28 5.4 At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase sm... 28 5.4 At2g44610.1 68415.m05553 Ras-related GTP-binding protein, putati... 27 7.1 At4g14905.2 68417.m02290 kelch repeat-containing F-box family pr... 27 9.4 At4g14905.1 68417.m02289 kelch repeat-containing F-box family pr... 27 9.4 At1g50030.1 68414.m05614 target of rapamycin protein (TOR) ident... 27 9.4 >At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2) identical to fatty acid multifunctional protein (AtMFP2) GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty acid beta-oxidation); contains Pfam profiles PF02737 (3-hydroxyacyl-CoA dehydrogenase, NAD binding domain), PF00378 (enoyl-CoA hydratase/isomerase family protein), PF00725 (3-hydroxyacyl-CoA dehydrogenase) Length = 725 Score = 68.1 bits (159), Expect = 4e-12 Identities = 40/137 (29%), Positives = 76/137 (55%), Gaps = 4/137 (2%) Frame = +2 Query: 131 LTVDNEGIAVLTMQRPPVNSLNLELLQDISKSLDD-VGKNKSKGMILASSSPTVFSAGLD 307 + V +G+AV+T+ PPVNSL+ ++L ++ + ++ + +N K +++ + FS G D Sbjct: 11 MEVGGDGVAVITLINPPVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGR-FSGGFD 69 Query: 308 IM---EMYKPDLKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEYRVM 478 I EM K ++K + + ++ + L + P+ AAI+G A GG +++C R+ Sbjct: 70 ISGFGEMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARIS 129 Query: 479 ANGKYSIGLNETALGIV 529 A +GL E LG++ Sbjct: 130 APAA-QLGLPELQLGVI 145 >At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty acid multifunctional protein (AIM1) identical to gi:4337025; contains Pfam profiles PF02737 (3-hydroxyacyl-CoA dehydrogenase, NAD binding domain), PF00378 (enoyl-CoA hydratase/isomerase family protein), PF00725 (3-hydroxyacyl-CoA dehydrogenase) Length = 721 Score = 61.7 bits (143), Expect = 4e-10 Identities = 47/148 (31%), Positives = 76/148 (51%), Gaps = 5/148 (3%) Frame = +2 Query: 101 MSAKAGPLVDLTVDNEGIAVLTMQRPPVNSLNLELLQDISKSLDDVG-KNKSKGMILASS 277 M+ K G V + V N+G+AV+T+ PPVNSL ++ + + D +N K ++L + Sbjct: 1 MAKKIG--VTMEVGNDGVAVITISNPPVNSLASPIISGLKEKFRDANQRNDVKAIVLIGN 58 Query: 278 SPTVFSAGLDI---MEMYKP-DLKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGC 445 + FS G DI +++K DL +++ V + S P AA+ G A GG Sbjct: 59 NGR-FSGGFDINVFQQVHKTGDLSLMPE--VSVELVCNLMEDSRKPVVAAVEGLALGGGL 115 Query: 446 LFSLSCEYRVMANGKYSIGLNETALGIV 529 +++C RV A K +GL E LG++ Sbjct: 116 ELAMACHARVAA-PKAQLGLPELTLGVI 142 >At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family protein similar to 3-hydroxybutyryl-CoA dehydratase (Crotonase) from Clostridium acetobutylicum [SP|P52046], FadB1x (enoyl-CoA hydratase) from Pseudomonas putida [GI:13310130]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 265 Score = 55.2 bits (127), Expect = 3e-08 Identities = 36/126 (28%), Positives = 68/126 (53%), Gaps = 4/126 (3%) Frame = +2 Query: 122 LVDLTVDNEGIAVLTMQRPP-VNSLNLELLQDISKSLDDVGKNKSKGMILASSSPTVFSA 298 L+ + ++ GIAV+T+ RP +NSL ++ D++K+ D+ ++S +++ + S F + Sbjct: 9 LIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCS 68 Query: 299 GLDIM---EMYKPDLKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEY 469 G+D+ ++K D+K ET D +++ P AING A G +L+C+ Sbjct: 69 GVDLTAAESVFKGDVKDPET------DPVVQMERLRKPIIGAINGFAITAGFELALACDI 122 Query: 470 RVMANG 487 V + G Sbjct: 123 LVASRG 128 >At1g65520.1 68414.m07434 enoyl-CoA hydratase/isomerase family protein low similarity to enoyl-CoA hydratase [Escherichia coli] GI:2764828, carnitine racemase SP|P31551 [Escherichia coli]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 240 Score = 54.8 bits (126), Expect = 4e-08 Identities = 30/121 (24%), Positives = 61/121 (50%), Gaps = 2/121 (1%) Frame = +2 Query: 191 LNLELLQDISKSLDDVGKNKS--KGMILASSSPTVFSAGLDIMEMYKPDLKRAETFWTAL 364 LN LL + +++ + + S + +++ +S FS G D+ + + + + L Sbjct: 23 LNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSNGYDLA-LAESNPSLSVVMDAKL 81 Query: 365 QDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEYRVMANGKYSIGLNETALGIVAPRWF 544 + + L PT AA+ GHA A GC+ ++S +Y +M + + ++E + ++ P WF Sbjct: 82 RSLVADLISLPMPTIAAVTGHASAAGCILAMSHDYVLMRRDRGFLYMSELDIELIVPAWF 141 Query: 545 M 547 M Sbjct: 142 M 142 >At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar to AU-binding protein/Enoyl-CoA hydratase [Homo sapiens] GI:780241, [Mus musculus]GI:6840920; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 229 Score = 52.4 bits (120), Expect = 2e-07 Identities = 31/110 (28%), Positives = 55/110 (50%), Gaps = 1/110 (0%) Frame = +2 Query: 203 LLQDISKSLDDVGK-NKSKGMILASSSPTVFSAGLDIMEMYKPDLKRAETFWTALQDVWL 379 +L+ + + + + + N ++ +++ S P VF AG D+ E T+ +L+ ++ Sbjct: 1 MLKSLQNAFESIHQDNSARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFS 60 Query: 380 KLYGSSFPTAAAINGHAPAGGCLFSLSCEYRVMANGKYSIGLNETALGIV 529 + S PT AAI G A GG +L+C+ R+ GL ET L I+ Sbjct: 61 FIEALSIPTIAAIEGAALGGGLEMALACDLRICGENAV-FGLPETGLAII 109 >At4g14440.1 68417.m02227 enoyl-CoA hydratase/isomerase family protein low similarity to PhaB [Pseudomonas putida] GI:3253198, SP|P31551 Carnitine racemase {Escherichia coli}; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 238 Score = 51.6 bits (118), Expect = 4e-07 Identities = 35/142 (24%), Positives = 65/142 (45%), Gaps = 5/142 (3%) Frame = +2 Query: 134 TVDNEG-IAVLTMQRPPVNSLNLELLQDISKSLDDVGKNKSKGMILASSSP-TVFSAGLD 307 T++ G + +LT+ + + + + + L+ +KG +L ++ FS G D Sbjct: 3 TLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNGFD 62 Query: 308 IMEMYKPD---LKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEYRVM 478 + +KR + + V L PT AA+NGHA A G +F+LS +Y M Sbjct: 63 LAWAQSAGHGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHDYVFM 122 Query: 479 ANGKYSIGLNETALGIVAPRWF 544 + + ++E +G+ P +F Sbjct: 123 RKDRGVLYMSEVDIGLPVPDYF 144 >At4g14430.1 68417.m02226 enoyl-CoA hydratase/isomerase family protein low siimilarity to 2-cyclohexenylcarbonyl CoA isomerase [Streptomyces collinus] GI:8133118, enoyl-CoA isomerase [Escherichia coli] GI:2764829; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 240 Score = 44.4 bits (100), Expect = 6e-05 Identities = 35/144 (24%), Positives = 64/144 (44%), Gaps = 7/144 (4%) Frame = +2 Query: 134 TVDNEG-IAVLTMQRPPVNSLNLELLQDISKSLDDVGKNKSKGMIL-ASSSPTVFSAGLD 307 T++ G + +LT+ + + + + I L+ ++G IL +++ FS G D Sbjct: 3 TLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNGFD 62 Query: 308 IMEMYKPDLK-----RAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEYR 472 + K R + + V L PT AA+NGHA A G + +LS +Y Sbjct: 63 LAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHDYV 122 Query: 473 VMANGKYSIGLNETALGIVAPRWF 544 M + + ++E +G+ P +F Sbjct: 123 FMRKDRGVLYMSEVDIGLSMPDYF 146 >At1g02790.1 68414.m00235 exopolygalacturonase / galacturan 1,4-alpha-galacturonidase (PGA3) / pectinase identical to SP|P49062 Exopolygalacturonase clone GBGE184 precursor (EC 3.2.1.67) (ExoPG) (Galacturan 1,4-alpha-galacturonidase) {Arabidopsis thaliana} Length = 422 Score = 36.3 bits (80), Expect = 0.015 Identities = 30/113 (26%), Positives = 51/113 (45%), Gaps = 2/113 (1%) Frame = +2 Query: 254 KGMILASSSPTVFSAGLDIMEMYKPDLKRAETFWTALQDVWLKLYGSSFPTAAAI-NGHA 430 +G+ S +PTV+ DI + TF A + W+++ S P + G Sbjct: 40 RGLAANSGNPTVY----DITKFGAVGDGSTNTF-KAFLNTWIQVCDSPVPATLLVPKGTF 94 Query: 431 PAGGCLFSLSCEYRVMANGKYSIG-LNETALGIVAPRWFMYSMVEHDLEQGSG 586 AG +F+ C+ +V N IG + T G P WF++ V++ + G+G Sbjct: 95 LAGPVIFAGPCKSKVTVN---VIGTIIATTSGYATPEWFLFERVDNLVLTGTG 144 >At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydroxynaphthoic acid synthetase, putative / DHNA synthetase, putative contains similarity to MENB from Escherichia coli [SP|P27290], Bacillus subtilis [SP|P23966]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 337 Score = 34.3 bits (75), Expect = 0.062 Identities = 25/117 (21%), Positives = 56/117 (47%), Gaps = 3/117 (2%) Frame = +2 Query: 143 NEGIAVLTMQRPPV-NSLNLELLQDISKSLDDVGKNKSKGMILASSSPT-VFSAGLD-IM 313 +EGIA +T+ RP N+ + ++++ ++ +D + S G+I+ + T F +G D + Sbjct: 84 DEGIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQAL 143 Query: 314 EMYKPDLKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFSLSCEYRVMAN 484 + + D+ +++ P A + G+A GG + + C+ + A+ Sbjct: 144 RTQDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAAD 200 >At4g32910.1 68417.m04683 expressed protein Length = 716 Score = 30.7 bits (66), Expect = 0.76 Identities = 15/48 (31%), Positives = 25/48 (52%) Frame = +2 Query: 200 ELLQDISKSLDDVGKNKSKGMILASSSPTVFSAGLDIMEMYKPDLKRA 343 +L + KSL D + +G++ S + S GLD + Y+ D KR+ Sbjct: 564 QLFDSVGKSLSDESLKQWEGLVELLGSESQISGGLDFLHKYR-DFKRS 610 >At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family protein similar to CHY1 [gi:8572760]; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 387 Score = 30.7 bits (66), Expect = 0.76 Identities = 27/136 (19%), Positives = 56/136 (41%), Gaps = 3/136 (2%) Frame = +2 Query: 152 IAVLTMQRP-PVNSLNLELLQDISKSLDDVGKNKSKGMILASSSPTVFSAGLDIMEMYKP 328 + + T+ RP +N ++ E++ +++ L+ K+ +IL + FSAG D+ Y Sbjct: 21 VRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLILIKGTGRAFSAGGDLKVFYHG 80 Query: 329 DLKRAETFWTALQDVWLKLYGSSF--PTAAAINGHAPAGGCLFSLSCEYRVMANGKYSIG 502 + + WL + ++ + +NG + GG + ++ V+ K Sbjct: 81 QESKDSCLEVVYRMYWLCYHIHTYKKTQVSLVNGISMGGGAALMVPMKFSVVTE-KTVFA 139 Query: 503 LNETALGIVAPRWFMY 550 E + G F Y Sbjct: 140 TPEASFGFHTDCGFSY 155 >At2g36450.1 68415.m04474 AP2 domain-containing protein low similarity to DREB1B GI:3738226 from [Arabidopsis thaliana]; contains Pfam profile PF00847: AP2 domain Length = 184 Score = 28.7 bits (61), Expect = 3.1 Identities = 16/53 (30%), Positives = 28/53 (52%) Frame = +2 Query: 149 GIAVLTMQRPPVNSLNLELLQDISKSLDDVGKNKSKGMILASSSPTVFSAGLD 307 G+A + N +N + +D+ + +V N ++GM+L+ PTVF A D Sbjct: 118 GVACMNSSYDNTN-MNGFMDEDLVFDMPNVLMNMAEGMLLSPPRPTVFDAAYD 169 >At1g66600.1 68414.m07568 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 241 Score = 28.3 bits (60), Expect = 4.1 Identities = 10/38 (26%), Positives = 23/38 (60%) Frame = +2 Query: 65 NNVKSAVPNCRLMSAKAGPLVDLTVDNEGIAVLTMQRP 178 N +K+ + NC++ S PL++ +D+ +A+ ++ P Sbjct: 21 NILKTVLDNCKVSSVSTEPLINTILDSFSLALSSVNSP 58 >At2g42570.1 68415.m05268 expressed protein Length = 367 Score = 27.9 bits (59), Expect = 5.4 Identities = 36/134 (26%), Positives = 57/134 (42%), Gaps = 13/134 (9%) Frame = +2 Query: 71 VKSAVPNCRLMSAKAGPLVDLTVDNEGIAVLTMQRPPVNSLNLELLQDISKSLDDVGK-N 247 + S VPN R + L LT + G+ +L + + LN+E + + K LD + + N Sbjct: 135 IHSWVPNTRYTLIRQKGLASLTFEEYGVTLLLYRTQFLVDLNVEKVGRVLK-LDSIKQGN 193 Query: 248 KSKGM-ILASSS-----PTVFSAGLDIME----MYKPDLKRAETFWTALQD--VWLKLYG 391 +GM +L +S T D ME +YK D+ R F+ + W+ Y Sbjct: 194 MWRGMDVLIFNSWHWWTHTEHIQPWDYMEDGNRLYK-DMNRLVAFYKGMTTWARWVNAYV 252 Query: 392 SSFPTAAAINGHAP 433 T NG +P Sbjct: 253 DPSKTKVFFNGVSP 266 >At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase small chain, putative / ribonucleotide reductase, putative similar to ribonucleotide reductase GI:4151068 from [Nicotiana tabacum] Length = 816 Score = 27.9 bits (59), Expect = 5.4 Identities = 29/104 (27%), Positives = 49/104 (47%) Frame = +2 Query: 275 SSPTVFSAGLDIMEMYKPDLKRAETFWTALQDVWLKLYGSSFPTAAAINGHAPAGGCLFS 454 +SPT+F+AG +P L + F ++D ++ + A I+ AGG S Sbjct: 201 ASPTLFNAGTP-----RPQL--SSCFLVCMKDDSIEGIYETLKECAVIS--KSAGGIGVS 251 Query: 455 LSCEYRVMANGKYSIGLNETALGIVAPRWFMYSMVEHDLEQGSG 586 + + + A G Y G N T+ GIV P +++ ++QG G Sbjct: 252 V---HNIRATGSYIRGTNGTSNGIV-PMLRVFNDTARYVDQGGG 291 >At2g44610.1 68415.m05553 Ras-related GTP-binding protein, putative similar to GTP-binding protein GI:623586 from [Nicotiana tabacum] ; contains an ADP-ribosylation factors family signature for proteins involved in protein trafficking Length = 208 Score = 27.5 bits (58), Expect = 7.1 Identities = 10/33 (30%), Positives = 20/33 (60%) Frame = -3 Query: 165 VSTAIPSLSTVKSTNGPALADINLQLGTADLTL 67 ++ A+P + T+ ST + D+NL+ A+ +L Sbjct: 166 IAAALPGMETLSSTKQEDMVDVNLKSSNANASL 198 >At4g14905.2 68417.m02290 kelch repeat-containing F-box family protein contains Pfam PF01344: Kelch motif (2 copies) and PF00646: F-box domain; similar to putative SKP1 interacting partner 6 (GP:21689879) (SKIP6) {Arabidopsis thaliana}|19798784|gb|AU230074.1|AU230074 Length = 372 Score = 27.1 bits (57), Expect = 9.4 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +3 Query: 300 VLI*WKCTNQI*SEQKRFGLHYKMYGLSYMAQAFLLQLPSMVTHQPEGAYFPYRASTE*W 479 VL + C NQI + G+ +GL + + MV H A ++ TE W Sbjct: 271 VLYGFYCMNQILAYDLVVGMGTVFWGLEGLPEGLQSCTGRMVNHGGRLAILFKKSPTEIW 330 Query: 480 RTE 488 RTE Sbjct: 331 RTE 333 >At4g14905.1 68417.m02289 kelch repeat-containing F-box family protein contains Pfam PF01344: Kelch motif (2 copies) and PF00646: F-box domain; similar to putative SKP1 interacting partner 6 (GP:21689879) (SKIP6) {Arabidopsis thaliana}|19798784|gb|AU230074.1|AU230074 Length = 372 Score = 27.1 bits (57), Expect = 9.4 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +3 Query: 300 VLI*WKCTNQI*SEQKRFGLHYKMYGLSYMAQAFLLQLPSMVTHQPEGAYFPYRASTE*W 479 VL + C NQI + G+ +GL + + MV H A ++ TE W Sbjct: 271 VLYGFYCMNQILAYDLVVGMGTVFWGLEGLPEGLQSCTGRMVNHGGRLAILFKKSPTEIW 330 Query: 480 RTE 488 RTE Sbjct: 331 RTE 333 >At1g50030.1 68414.m05614 target of rapamycin protein (TOR) identical to pTOR [Arabidopsis thaliana] GI:12002902; contains Pfam profiles PF00454 Phosphatidylinositol 3- and 4-kinase, PF02259 FAT domain, PF02260 FATC domain Length = 2481 Score = 27.1 bits (57), Expect = 9.4 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = -3 Query: 162 STAIPSLSTVKSTNGPALADINLQLGTADLTLLIK*RKGSI 40 S + S+ + K N P LA +NL+LGT L GSI Sbjct: 1695 SDILASMVSSKGANVPLLARVNLKLGTWQWALSSGLNDGSI 1735 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,623,306 Number of Sequences: 28952 Number of extensions: 256763 Number of successful extensions: 625 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 608 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 620 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1171109464 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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