Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_H20
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 105 2e-23
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 104 3e-23
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 99 1e-21
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 97 5e-21
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 72 2e-13
At4g39060.1 68417.m05532 F-box family protein low similarity to ... 30 0.69
At3g47500.1 68416.m05166 Dof-type zinc finger domain-containing ... 29 2.1
At1g66950.1 68414.m07612 ABC transporter family protein similar ... 28 2.8
At5g37540.1 68418.m04521 aspartyl protease family protein weak s... 28 3.7
At4g19330.1 68417.m02848 kelch repeat-containing F-box family pr... 27 6.4
At5g45250.1 68418.m05553 disease resistance protein (TIR-NBS-LRR... 27 8.5
At4g16070.1 68417.m02437 lipase class 3 family protein low simil... 27 8.5
At2g35340.1 68415.m04333 RNA helicase, putative similar to ATP-d... 27 8.5
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 105 bits (252), Expect = 2e-23
Identities = 56/130 (43%), Positives = 75/130 (57%)
Frame = +1
Query: 1 AGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPG 180
AG AA+ TV LD GG AVQHRE GHES+ FL YF + L+GG SGF V
Sbjct: 76 AGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGFK-TVEEEV 134
Query: 181 AEKRMFQVKGERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
E R++ KG+R +R++QV SS+N D FILD IY + G + E+ KA+ +
Sbjct: 135 FETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNGANSNIQERAKALEVV 194
Query: 361 NQIRDQDHNG 390
++D+ H G
Sbjct: 195 QYLKDKYHEG 204
Score = 29.1 bits (62), Expect = 1.6
Identities = 27/124 (21%), Positives = 53/124 (42%), Gaps = 10/124 (8%)
Frame = +1
Query: 31 LDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGAEKRMF---- 198
+ + G VQ R G E F+ F + L GG SG+ + + +
Sbjct: 459 MSNSLKGRPVQGRIYEGKEPPQFVALF-QPMVVLKGGLSSGYKSSMGESESTDETYTPES 517
Query: 199 ----QVKGE--RNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
QV G N + QV+ + +S+N +CF+L ++++ G ++ + + A +A
Sbjct: 518 IALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHGNQSTHEQLELATKVA 577
Query: 361 NQIR 372
++
Sbjct: 578 EFLK 581
Score = 28.3 bits (60), Expect = 2.8
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1
Query: 250 SSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
S + C++LD +IY++VG R V++ KA S +
Sbjct: 272 SMLENTKCYLLDCGAEIYIWVG-RVTQVDERKAASQS 307
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 104 bits (250), Expect = 3e-23
Identities = 53/131 (40%), Positives = 78/131 (59%)
Frame = +1
Query: 1 AGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPG 180
AGA A++TV LD GG AVQ+RE GHE+ FL YF + +GG SGFNHV
Sbjct: 78 AGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFNHVKPEE- 136
Query: 181 AEKRMFQVKGERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
+ R++ KG+ VRV++V + S++N D FILD I+ + G ++ E+ KA+ +
Sbjct: 137 HQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVV 196
Query: 361 NQIRDQDHNGR 393
I+D H+G+
Sbjct: 197 QYIKDTYHDGK 207
Score = 30.7 bits (66), Expect = 0.52
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +1
Query: 268 DCFILDIHYDIYVYVGERTKNVEKLKAISMANQIRDQD 381
D FILD H +++V+VG++ +K +A+ + D
Sbjct: 653 DIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLKHD 690
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 99.1 bits (236), Expect = 1e-21
Identities = 55/137 (40%), Positives = 76/137 (55%)
Frame = +1
Query: 1 AGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPG 180
AG AA+ TV LD GG AVQ+RE GHES+ FL YF + L+GG SGF
Sbjct: 78 AGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGFKK-PEEEE 136
Query: 181 AEKRMFQVKGERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
E R++ KG+R V ++QV SS+N D FILD IY + G + E+ KA+ +
Sbjct: 137 FETRLYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNGANSNIQERAKALVVI 196
Query: 361 NQIRDQDHNGRGRVELL 411
++D+ H G V ++
Sbjct: 197 QYLKDKFHEGTSDVAIV 213
Score = 41.1 bits (92), Expect = 4e-04
Identities = 33/131 (25%), Positives = 59/131 (45%), Gaps = 10/131 (7%)
Frame = +1
Query: 10 AAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGAEK 189
A L + + G VQ R G E F+ F + L GG SG+ + +T G+
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQHMV-VLKGGLSSGYKNSMTEKGSSG 512
Query: 190 RMF--------QVKGE--RNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEK 339
+ QV G N + QV+ + +S+N DCF+L ++++VG + + ++
Sbjct: 513 ETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVGNHSTHEQQ 572
Query: 340 LKAISMANQIR 372
A +A ++
Sbjct: 573 ELAAKVAEFLK 583
Score = 28.3 bits (60), Expect = 2.8
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +1
Query: 250 SSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
S + C++LD +I+++VG T+ E+ AI A
Sbjct: 274 SMLENNKCYLLDCGSEIFIWVGRVTQVEERKTAIQAA 310
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 97.1 bits (231), Expect = 5e-21
Identities = 52/130 (40%), Positives = 72/130 (55%)
Frame = +1
Query: 1 AGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPG 180
AG AA+ TV LD GG AVQ+RE GHE+ FL YF + +GG SGF HVV
Sbjct: 78 AGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFKHVVAEEH 137
Query: 181 AEKRMFQVKGERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMA 360
R+F +G+ V V++V SS+N D +ILD I+ + G + E+ KA+ +
Sbjct: 138 I-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVV 196
Query: 361 NQIRDQDHNG 390
I+D H+G
Sbjct: 197 QYIKDTYHDG 206
Score = 32.7 bits (71), Expect = 0.13
Identities = 13/38 (34%), Positives = 24/38 (63%)
Frame = +1
Query: 268 DCFILDIHYDIYVYVGERTKNVEKLKAISMANQIRDQD 381
D FI+D H +I+V+VG+ KL A+++ + ++D
Sbjct: 651 DIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEKFIEKD 688
Score = 28.3 bits (60), Expect = 2.8
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +1
Query: 199 QVKGERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVG 315
Q G N++ QVDP+ +S+N +IL ++ + G
Sbjct: 525 QGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 72.1 bits (169), Expect = 2e-13
Identities = 43/141 (30%), Positives = 69/141 (48%), Gaps = 2/141 (1%)
Frame = +1
Query: 31 LDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGAEKRMFQVKG 210
LD G VQ+RE G E+ FL YF + ++G + + + + KG
Sbjct: 88 LDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKYSPKTG--IAGETYQVTLLRCKG 145
Query: 211 ERNVRVRQVDPLISSMNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMANQIRDQDHNG 390
+ VRV++V L SS+N D FILD ++++ G + EK KA+ + I+D H+G
Sbjct: 146 DHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSSTQEKAKAMEVVEYIKDNKHDG 205
Query: 391 RGRVELL--AHYSNEVDTLKF 447
R V + +S + D +F
Sbjct: 206 RCEVATIEDGKFSGDSDAGEF 226
Score = 32.3 bits (70), Expect = 0.17
Identities = 14/64 (21%), Positives = 33/64 (51%)
Frame = +1
Query: 256 MNQGDCFILDIHYDIYVYVGERTKNVEKLKAISMANQIRDQDHNGRGRVELLAHYSNEVD 435
+ + C++LD H +++V++G T E+ +IS + + ++ GR L + ++
Sbjct: 277 LEKNKCYMLDCHSEVFVWMGRNTSLTERKTSISSSEEFLRKE--GRSTTTSLVLLTEGLE 334
Query: 436 TLKF 447
+F
Sbjct: 335 NARF 338
Score = 27.9 bits (59), Expect = 3.7
Identities = 10/38 (26%), Positives = 22/38 (57%)
Frame = +1
Query: 268 DCFILDIHYDIYVYVGERTKNVEKLKAISMANQIRDQD 381
D F+LD ++YV++G + K +A+++ + + D
Sbjct: 648 DVFLLDCQSEVYVWIGSNSNIKSKEEALTLGLKFLEMD 685
>At4g39060.1 68417.m05532 F-box family protein low similarity to
SKP1 interacting partner 6 [Arabidopsis thaliana]
GI:10716957; contains Pfam profile PF00646: F-box domain
Length = 375
Score = 30.3 bits (65), Expect = 0.69
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -3
Query: 179 PGFVTTWLKPDGLPPSKYCSADVKYCRKWLLSWPIASRCCTAAPPNL 39
P + + W+KPD ++YC+ K R L+ P+ S T PPNL
Sbjct: 64 PIWFSLWIKPDDQTLNQYCNIQEKSTRNLLV--PVPS-SYTPFPPNL 107
>At3g47500.1 68416.m05166 Dof-type zinc finger domain-containing
protein identical to H-protein promoter binding
factor-2a GI:3386546 from [Arabidopsis thaliana]
Length = 448
Score = 28.7 bits (61), Expect = 2.1
Identities = 18/61 (29%), Positives = 21/61 (34%)
Frame = -3
Query: 209 PLTWNIRFSAPGFVTTWLKPDGLPPSKYCSADVKYCRKWLLSWPIASRCCTAAPPNLSSR 30
P TWN PGF P G P Y + S PI+ +C P L
Sbjct: 296 PYTWNPAMPPPGF----YPPPGYPMPFYPYWTIPMLPPHQSSSPISQKCSNTNSPTLGKH 351
Query: 29 P 27
P
Sbjct: 352 P 352
>At1g66950.1 68414.m07612 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]
Length = 1454
Score = 28.3 bits (60), Expect = 2.8
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +1
Query: 49 GAAVQHREAMGHESNHFLQYFTS---ALQYLDGGNPSGFNHVVTNPGAEKRM 195
G V + ++GH S ++YF + + DG NP+ + VT P E +M
Sbjct: 1081 GGQVIYAGSLGHHSQKLVEYFEAVEGVPKINDGYNPATWMLDVTTPSMESQM 1132
>At5g37540.1 68418.m04521 aspartyl protease family protein weak
similarity to CND41, chloroplast nucleoid DNA binding
protein [Nicotiana tabacum] GI:2541876; contains Prosite
PS00141: Eukaryotic and viral aspartyl proteases active
site; contains 1 predicted transmembrane domain
Length = 442
Score = 27.9 bits (59), Expect = 3.7
Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = -2
Query: 324 SSFAHINVYVVMYVKDEAVALIHGRYQWVYLSHSNIPLAFNLEHTF-LGTRIRHDVVETG 148
S F H+ VK+E V L+ R + Y+ S + F+ H+ +G I V E G
Sbjct: 315 SEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLIGDLVFEFG 374
Query: 147 RVTPIQV 127
R I V
Sbjct: 375 RGVEILV 381
>At4g19330.1 68417.m02848 kelch repeat-containing F-box family
protein very low similarity to SKP1 interacting partner
6 [Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 537
Score = 27.1 bits (57), Expect = 6.4
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = -3
Query: 161 WLKPDGLPPSKYCSADVKYCRKWLLSWPIA-SRC 63
W+KP P + + D+K WLL P SRC
Sbjct: 248 WIKPYDHQPLTHWTIDIKCTGHWLLPMPSPYSRC 281
>At5g45250.1 68418.m05553 disease resistance protein (TIR-NBS-LRR
class), putative (RPS4) domain signature TIR-NBS-LRR
exists, suggestive of a disease resistance protein.
Identical to RPS4 (GI:11357255). False intron created at
intron 2 to escape a frameshift in the BAC sequence.
Length = 1217
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = -2
Query: 66 VLYRRSTELVIEADSQDGSRSR 1
+LY+ S E+ ++A +QDGSR R
Sbjct: 510 LLYKFSREVDLKASNQDGSRQR 531
>At4g16070.1 68417.m02437 lipase class 3 family protein low
similarity to calmodulin-binding heat-shock protein
CaMBP [Nicotiana tabacum] GI:1087073; contains Pfam
profile PF01764: Lipase, PF03893: Lipase 3 N-terminal
region
Length = 654
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/40 (27%), Positives = 21/40 (52%)
Frame = -3
Query: 251 DISGSTCLTRTFLSPLTWNIRFSAPGFVTTWLKPDGLPPS 132
+ + +TC T + +TW++ S F+TT + L P+
Sbjct: 277 EFASATCFTFAPAACMTWDLAESGKHFITTIINGSDLVPT 316
>At2g35340.1 68415.m04333 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1110
Score = 26.6 bits (56), Expect = 8.5
Identities = 20/62 (32%), Positives = 28/62 (45%), Gaps = 4/62 (6%)
Frame = +1
Query: 166 VTNPGAEKRMFQVKGERNVRVR--QVDPLISSMNQGDCF--ILDIHYDIYVYVGERTKNV 333
V A K KG++ R + Q++ N D F + D HY IYV V E +NV
Sbjct: 125 VKKQSASKTGKSDKGQKRFRKKSEQLEDDDDEANYFDIFYSMTDFHYFIYVIVREDKRNV 184
Query: 334 EK 339
+
Sbjct: 185 RR 186
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,141,032
Number of Sequences: 28952
Number of extensions: 239718
Number of successful extensions: 624
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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