Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_H17
(556 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 99 2e-21
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 96 1e-20
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 94 5e-20
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 91 5e-19
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.22
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 1.6
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 28 3.6
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 27 6.4
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 98.7 bits (235), Expect = 2e-21
Identities = 56/177 (31%), Positives = 83/177 (46%), Gaps = 3/177 (1%)
Frame = +2
Query: 35 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 214
+ D+ T +G+ + ++LA ++Y+SG S D+KV+ V P+ + P+ +WY +A
Sbjct: 3 VTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVA 62
Query: 215 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX---LFGSGXXXXXXXXXXXXX 385
S + GV + LFG
Sbjct: 63 SQLAKSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE--- 119
Query: 386 XXLKAYADKKSKKPALIAKSSIILDVKPWDDETDLKDMENQVRTIEMDGLLWGASKL 556
+ A K +KKP KSS+++DVKPWDDETD+K +E VR +EM GL WGASKL
Sbjct: 120 ---REAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKL 173
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 96.3 bits (229), Expect = 1e-20
Identities = 62/184 (33%), Positives = 87/184 (47%), Gaps = 11/184 (5%)
Frame = +2
Query: 38 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI-- 211
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + +V RW++HI
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 212 ---ASYTPAERK-TWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 364
S AE EG S S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGE-ETEEEK 121
Query: 365 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVKPWDDETDLKDMENQVRTIEMDGLLWG 544
+KA KK KSS+++D+KPWDDETD+K +E VR+I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 545 ASKL 556
ASKL
Sbjct: 177 ASKL 180
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 94.3 bits (224), Expect = 5e-20
Identities = 54/175 (30%), Positives = 82/175 (46%), Gaps = 1/175 (0%)
Frame = +2
Query: 35 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 214
+ D+ T +GL L ++LA ++Y+SG S D+KV+ V + P P+ +WY +A
Sbjct: 3 VTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVA 62
Query: 215 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXXL 394
S+ ++GV +
Sbjct: 63 SHLAKSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAE 122
Query: 395 KAYADKK-SKKPALIAKSSIILDVKPWDDETDLKDMENQVRTIEMDGLLWGASKL 556
+ A KK +KK KSS++L+VKPWDDETD+K +E VR+++M GL WGASKL
Sbjct: 123 EREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKL 177
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 91.1 bits (216), Expect = 5e-19
Identities = 57/184 (30%), Positives = 85/184 (46%), Gaps = 11/184 (5%)
Frame = +2
Query: 38 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSH--- 208
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + + RWY+H
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 209 ---IASYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 364
I+ + EG + S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGE-ETEEEK 121
Query: 365 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVKPWDDETDLKDMENQVRTIEMDGLLWG 544
+KA KK KSS+++D+KPWDDETD+K +E V++I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 545 ASKL 556
ASKL
Sbjct: 177 ASKL 180
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 32.3 bits (70), Expect = 0.22
Identities = 22/57 (38%), Positives = 32/57 (56%), Gaps = 9/57 (15%)
Frame = +2
Query: 68 LNELNQYLAERSYV--SGYTPSQADIKVFEQV-GKVPAAS------LPHVLRWYSHI 211
L +LN LA +S + +G TPS AD+ VF + V S +PHV+RW ++I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 29.5 bits (63), Expect = 1.6
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +2
Query: 413 KSKKPALIAKSSIILDVKPWDDETDLKDMENQVRTIEMDGLLWG 544
K KP +I K+SI+L + D+E LK+ +R I++D L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 28.3 bits (60), Expect = 3.6
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 68 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASL 181
+N + + +R V G TP+QAD++V VG + A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.5 bits (58), Expect = 6.4
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 68 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKV 166
L +L Y YVS PSQA + + EQ GK+
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGKI 526
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,446,531
Number of Sequences: 28952
Number of extensions: 178338
Number of successful extensions: 415
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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