BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_H12 (628 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g04710.1 68414.m00468 acetyl-CoA C-acyltransferase, putative ... 159 1e-39 At2g33150.1 68415.m04062 acetyl-CoA C-acyltransferase, putative ... 154 4e-38 At5g48880.2 68418.m06047 acetyl-CoA C-acyltransferase 1 / 3-keto... 143 7e-35 At5g48880.1 68418.m06046 acetyl-CoA C-acyltransferase 1 / 3-keto... 143 7e-35 At5g48230.2 68418.m05959 acetyl-CoA C-acyltransferase, putative ... 100 9e-22 At5g48230.1 68418.m05958 acetyl-CoA C-acyltransferase, putative ... 100 9e-22 At5g47720.4 68418.m05895 acetyl-CoA C-acyltransferase, putative ... 99 1e-21 At5g47720.3 68418.m05894 acetyl-CoA C-acyltransferase, putative ... 99 1e-21 At5g47720.2 68418.m05896 acetyl-CoA C-acyltransferase, putative ... 99 1e-21 At5g47720.1 68418.m05893 acetyl-CoA C-acyltransferase, putative ... 99 1e-21 At5g50890.1 68418.m06308 hypothetical protein 29 2.5 At2g22470.1 68415.m02664 arabinogalactan-protein (AGP2) identica... 29 2.5 At1g77420.1 68414.m09016 hydrolase, alpha/beta fold family prote... 29 2.5 At5g46290.1 68418.m05698 3-oxoacyl-[acyl-carrier-protein] syntha... 28 5.8 At4g20690.1 68417.m03007 hypothetical protein 28 5.8 At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-contain... 27 7.7 >At1g04710.1 68414.m00468 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative similar to 3-ketoacyl-CoA thiolase (E.C. 2.3.1.16) from [Arabidopsis thaliana] GI:2981616, [Cucumis sativus] GI:393707, [Cucurbita cv. Kurokawa Amakuri] GI:1694621; contains InterPro accession IPR002155: Thiolase Length = 443 Score = 159 bits (386), Expect = 1e-39 Identities = 84/178 (47%), Positives = 106/178 (59%), Gaps = 4/178 (2%) Frame = +3 Query: 6 VDEHPRPQTTLEGLKKLPPVFKKEGLVTXXXXXXXXXXXXXLVLASEEAA--KNLKPLAR 179 VD+ RP TTL GL KL PVFK++G T ++L A K L L Sbjct: 252 VDDGIRPNTTLSGLAKLKPVFKEDGTTTAGNSSQLSDGAGAVLLMRRNVAMQKGLPILGV 311 Query: 180 FVGWSYVGVDPSIMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDM 359 F +S VGVDP+IMG+GP AI +K + LND+DL EIN AF +Q + C L LD Sbjct: 312 FRTFSAVGVDPAIMGVGPAVAIPAAVKAAGLELNDVDLFEINEAFASQFVYCRNKLGLDA 371 Query: 360 QKLNVNGGATALGHPLGASGSRITAHLVHELRRRGLKRAIG--SACIGGGQGIAVMIE 527 +K+NVNGGA A+GHPLGA+G+R A L+HE++RRG G S CIG G G A + E Sbjct: 372 EKINVNGGAIAIGHPLGATGARCVATLLHEMKRRGKDCRFGVVSMCIGSGMGAAAVFE 429 >At2g33150.1 68415.m04062 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative similar to 3-ketoacyl-CoA thiolase (E.C. 2.3.1.16) from [Arabidopsis thaliana] GI:2981616, [Cucumis sativus] GI:393707, [Cucurbita cv. Kurokawa Amakuri] GI:1694621; contains InterPro accession IPR002155: Thiolase Length = 462 Score = 154 bits (374), Expect = 4e-38 Identities = 84/178 (47%), Positives = 105/178 (58%), Gaps = 4/178 (2%) Frame = +3 Query: 6 VDEHPRPQTTLEGLKKLPPVFKKEGLVTXXXXXXXXXXXXXLVLASEEAA--KNLKPLAR 179 VD+ RP TTL L KL PVFKK+G T ++L A K L L Sbjct: 260 VDDGIRPTTTLASLGKLKPVFKKDGTTTAGNSSQVSDGAGAVLLMKRSVAMQKGLPVLGV 319 Query: 180 FVGWSYVGVDPSIMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDM 359 F ++ VGVDP+IMGIGP AI +K + L+DIDL EIN AF +Q + C L LD Sbjct: 320 FRTFAAVGVDPAIMGIGPAVAIPAAVKAAGLELDDIDLFEINEAFASQFVYCRNKLGLDP 379 Query: 360 QKLNVNGGATALGHPLGASGSRITAHLVHELRRRGLKRAIG--SACIGGGQGIAVMIE 527 +K+NVNGGA A+GHPLGA+G+R A L+HE++RRG G S CIG G G A + E Sbjct: 380 EKINVNGGAMAIGHPLGATGARCVATLLHEMKRRGKDCRFGVVSMCIGTGMGAAAVFE 437 >At5g48880.2 68418.m06047 acetyl-CoA C-acyltransferase 1 / 3-ketoacyl-CoA thiolase 1 (PKT1) identical to 3-keto-acyl-CoA-thiolase 1 [Arabidopsis thaliana] GI:3169568 Length = 457 Score = 143 bits (347), Expect = 7e-35 Identities = 77/178 (43%), Positives = 103/178 (57%), Gaps = 4/178 (2%) Frame = +3 Query: 6 VDEHPRPQTTLEGLKKLPPVFKKEGLVTXXXXXXXXXXXXXLVLASEEAA--KNLKPLAR 179 VD+ RP + + L KL VFK+ G T ++L A K L L Sbjct: 261 VDDGVRPNSNMADLAKLKTVFKQNGSTTAGNASQISDGAGAVLLMKRSLAMKKGLPILGV 320 Query: 180 FVGWSYVGVDPSIMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDM 359 F ++ GV+PS+MGIGP AI K+ + ++DIDL EIN AF +Q + K L+LDM Sbjct: 321 FRSFAVTGVEPSVMGIGPAVAIPAATKLAGLNVSDIDLFEINEAFASQYVYSCKKLELDM 380 Query: 360 QKLNVNGGATALGHPLGASGSRITAHLVHELRRRGLKRAIG--SACIGGGQGIAVMIE 527 +K+NVNGGA A+GHPLGA+G+R A L+HE++RRG G S CIG G G A + E Sbjct: 381 EKVNVNGGAIAIGHPLGATGARCVATLLHEMKRRGKDCRFGVISMCIGTGMGAAAVFE 438 >At5g48880.1 68418.m06046 acetyl-CoA C-acyltransferase 1 / 3-ketoacyl-CoA thiolase 1 (PKT1) identical to 3-keto-acyl-CoA-thiolase 1 [Arabidopsis thaliana] GI:3169568 Length = 414 Score = 143 bits (347), Expect = 7e-35 Identities = 77/178 (43%), Positives = 103/178 (57%), Gaps = 4/178 (2%) Frame = +3 Query: 6 VDEHPRPQTTLEGLKKLPPVFKKEGLVTXXXXXXXXXXXXXLVLASEEAA--KNLKPLAR 179 VD+ RP + + L KL VFK+ G T ++L A K L L Sbjct: 218 VDDGVRPNSNMADLAKLKTVFKQNGSTTAGNASQISDGAGAVLLMKRSLAMKKGLPILGV 277 Query: 180 FVGWSYVGVDPSIMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDM 359 F ++ GV+PS+MGIGP AI K+ + ++DIDL EIN AF +Q + K L+LDM Sbjct: 278 FRSFAVTGVEPSVMGIGPAVAIPAATKLAGLNVSDIDLFEINEAFASQYVYSCKKLELDM 337 Query: 360 QKLNVNGGATALGHPLGASGSRITAHLVHELRRRGLKRAIG--SACIGGGQGIAVMIE 527 +K+NVNGGA A+GHPLGA+G+R A L+HE++RRG G S CIG G G A + E Sbjct: 338 EKVNVNGGAIAIGHPLGATGARCVATLLHEMKRRGKDCRFGVISMCIGTGMGAAAVFE 395 >At5g48230.2 68418.m05959 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 403 Score = 100 bits (239), Expect = 9e-22 Identities = 60/164 (36%), Positives = 82/164 (50%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNLK--PLARFVGWSYVGVDPS 215 L+KL P FK+ G VT LVL S E A L LA+ G+ +P Sbjct: 238 LRKLRPSFKENGGTVTAGNASSISDGAAALVLVSGEKALQLGLLVLAKIKGYGDAAQEPE 297 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 P AI + + + +D EIN AF L+ K L + +K+NVNGGA +L Sbjct: 298 FFTTAPALAIPKAIAHAGLESSQVDYYEINEAFAVVALANQKLLGIAPEKVNVNGGAVSL 357 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ L++R K +G C GGG A+++E Sbjct: 358 GHPLGCSGARILITLLGILKKRNGKYGVGGVCNGGGGASALVLE 401 >At5g48230.1 68418.m05958 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 398 Score = 100 bits (239), Expect = 9e-22 Identities = 60/164 (36%), Positives = 82/164 (50%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNLK--PLARFVGWSYVGVDPS 215 L+KL P FK+ G VT LVL S E A L LA+ G+ +P Sbjct: 233 LRKLRPSFKENGGTVTAGNASSISDGAAALVLVSGEKALQLGLLVLAKIKGYGDAAQEPE 292 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 P AI + + + +D EIN AF L+ K L + +K+NVNGGA +L Sbjct: 293 FFTTAPALAIPKAIAHAGLESSQVDYYEINEAFAVVALANQKLLGIAPEKVNVNGGAVSL 352 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ L++R K +G C GGG A+++E Sbjct: 353 GHPLGCSGARILITLLGILKKRNGKYGVGGVCNGGGGASALVLE 396 >At5g47720.4 68418.m05895 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 406 Score = 99 bits (238), Expect = 1e-21 Identities = 61/164 (37%), Positives = 84/164 (51%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNL--KPLARFVGWSYVGVDPS 215 LKKL P FK++G VT LVL S E A L +A+ G++ P Sbjct: 241 LKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKIRGYADAAQAPE 300 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 + P AI +K + + +D EIN AF L+ K L LD ++LN +GGA +L Sbjct: 301 LFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLLGLDPERLNAHGGAVSL 360 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ LR + K + S C GGG A+++E Sbjct: 361 GHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 404 >At5g47720.3 68418.m05894 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 405 Score = 99 bits (238), Expect = 1e-21 Identities = 61/164 (37%), Positives = 84/164 (51%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNL--KPLARFVGWSYVGVDPS 215 LKKL P FK++G VT LVL S E A L +A+ G++ P Sbjct: 240 LKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKIRGYADAAQAPE 299 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 + P AI +K + + +D EIN AF L+ K L LD ++LN +GGA +L Sbjct: 300 LFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLLGLDPERLNAHGGAVSL 359 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ LR + K + S C GGG A+++E Sbjct: 360 GHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403 >At5g47720.2 68418.m05896 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 415 Score = 99 bits (238), Expect = 1e-21 Identities = 61/164 (37%), Positives = 84/164 (51%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNL--KPLARFVGWSYVGVDPS 215 LKKL P FK++G VT LVL S E A L +A+ G++ P Sbjct: 240 LKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKIRGYADAAQAPE 299 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 + P AI +K + + +D EIN AF L+ K L LD ++LN +GGA +L Sbjct: 300 LFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLLGLDPERLNAHGGAVSL 359 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ LR + K + S C GGG A+++E Sbjct: 360 GHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403 >At5g47720.1 68418.m05893 acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative strong similarity to Acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9) [Raphanus sativus] GI:1542941; contains InterPro accession IPR002155: Thiolase Length = 405 Score = 99 bits (238), Expect = 1e-21 Identities = 61/164 (37%), Positives = 84/164 (51%), Gaps = 3/164 (1%) Frame = +3 Query: 45 LKKLPPVFKKEG-LVTXXXXXXXXXXXXXLVLASEEAAKNL--KPLARFVGWSYVGVDPS 215 LKKL P FK++G VT LVL S E A L +A+ G++ P Sbjct: 240 LKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKIRGYADAAQAPE 299 Query: 216 IMGIGPVPAIENLLKVTNMTLNDIDLIEINVAFVAQTLSCAKALKLDMQKLNVNGGATAL 395 + P AI +K + + +D EIN AF L+ K L LD ++LN +GGA +L Sbjct: 300 LFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLLGLDPERLNAHGGAVSL 359 Query: 396 GHPLGASGSRITAHLVHELRRRGLKRAIGSACIGGGQGIAVMIE 527 GHPLG SG+RI L+ LR + K + S C GGG A+++E Sbjct: 360 GHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403 >At5g50890.1 68418.m06308 hypothetical protein Length = 408 Score = 29.1 bits (62), Expect = 2.5 Identities = 19/64 (29%), Positives = 29/64 (45%) Frame = -1 Query: 301 ISIRSISLRVMLVTLRRFSMAGTGPMPMMLGSTPTYDQPTNRASGFRFLAASSLASTRAP 122 +S+R+IS + + R SM P++ S P+N +SGFR S S + P Sbjct: 247 MSLRNISEKAGVAWKRLMSMLPPKHEPLIQNSNEGQVSPSNVSSGFRNWVPSFYGSNQKP 306 Query: 121 APSL 110 L Sbjct: 307 TVDL 310 >At2g22470.1 68415.m02664 arabinogalactan-protein (AGP2) identical to gi|3883122|gb|AAC77824; supported by cDNA gi|3883121|gb|AF082299 Length = 131 Score = 29.1 bits (62), Expect = 2.5 Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 3/29 (10%) Frame = -1 Query: 160 FLAASSLASTRAPAPSLI---PDALPAVT 83 FLA S LA APAP+ + P ALP VT Sbjct: 14 FLATSCLAQAPAPAPTTVTPPPTALPPVT 42 >At1g77420.1 68414.m09016 hydrolase, alpha/beta fold family protein similar to monoglyceride lipase from [Homo sapiens] GI:14594904, [Mus musculus] GI:2632162; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 382 Score = 29.1 bits (62), Expect = 2.5 Identities = 15/33 (45%), Positives = 20/33 (60%) Frame = -1 Query: 205 TPTYDQPTNRASGFRFLAASSLASTRAPAPSLI 107 TP +D P S FR+ SSLA TR AP+++ Sbjct: 6 TPRFDTPAT-LSPFRYRYGSSLAITRRFAPAIV 37 >At5g46290.1 68418.m05698 3-oxoacyl-[acyl-carrier-protein] synthase I identical to Swiss-Prot:P52410 3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplast precursor (EC 2.3.1.41) (Beta-ketoacyl-ACP synthase I) (KAS I) [Arabidopsis thaliana] Length = 473 Score = 27.9 bits (59), Expect = 5.8 Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 4/75 (5%) Frame = +3 Query: 207 DPSIMGIGPVPAIENLLKVTNMTLNDIDLIEINV-AFVAQTLSCAKALKLDMQK---LNV 374 DP G+G IE L+ ++ +++ I + + +A L+ A+K + + + Sbjct: 332 DPRADGLGVSSCIERCLEDAGVSPEEVNYINAHATSTLAGDLAEINAIKKVFKSTSGIKI 391 Query: 375 NGGATALGHPLGASG 419 N + +GH LGA+G Sbjct: 392 NATKSMIGHCLGAAG 406 >At4g20690.1 68417.m03007 hypothetical protein Length = 138 Score = 27.9 bits (59), Expect = 5.8 Identities = 17/66 (25%), Positives = 30/66 (45%) Frame = -1 Query: 232 GPMPMMLGSTPTYDQPTNRASGFRFLAASSLASTRAPAPSLIPDALPAVTRPSFLKTGGS 53 G + S+ QP + + L + S++S+ + PS A +TRP +L + Sbjct: 11 GHLETKTNSSDRPSQPARHLTRLKHLVSRSVSSSSSARPS---QAARQLTRPKYLASRPI 67 Query: 52 FFRPSS 35 FRP + Sbjct: 68 VFRPQA 73 >At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-containing protein similar to SP|Q06881 Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP) {Anabaena sp.}; contains Pfam profile PF00364: Biotin-requiring enzyme Length = 274 Score = 27.5 bits (58), Expect = 7.7 Identities = 16/57 (28%), Positives = 21/57 (36%) Frame = -1 Query: 178 RASGFRFLAASSLASTRAPAPSLIPDALPAVTRPSFLKTGGSFFRPSSVVCGRGCSS 8 + GFR A L +P P I + A P + T GS S + SS Sbjct: 113 KLGGFRLYVARKLTDESSPPPQQIQPVVAASATPEGVHTNGSATSSSLAITKTSSSS 169 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,205,561 Number of Sequences: 28952 Number of extensions: 263281 Number of successful extensions: 888 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 845 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 876 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1275599520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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