Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_F17
(528 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 149 2e-36
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 53 1e-07
At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) f... 29 1.5
At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containi... 28 3.4
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 28 4.5
At5g01190.1 68418.m00024 laccase, putative / diphenol oxidase, p... 28 4.5
At1g07510.1 68414.m00804 FtsH protease, putative similar to AAA-... 28 4.5
At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,... 27 5.9
At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,... 27 5.9
At3g27880.1 68416.m03476 expressed protein 27 7.8
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 149 bits (360), Expect = 2e-36
Identities = 68/122 (55%), Positives = 87/122 (71%)
Frame = +1
Query: 163 GMVKSIHGTYKVKYRPDGPSGEEVEIDFTPPFARVPMIASLEKILNVKLPSPDTLDTAES 342
GMVK + G YK+KY +G + +EIDFTPPF R+ MI LEK+ KL P L + E+
Sbjct: 396 GMVKELTGGYKIKYNANGYDKDPIEIDFTPPFRRIEMIGELEKV--AKLNIPKDLASEEA 453
Query: 343 NALLSQLCEKHEVECPPPRTTARLLDKLVGEFLEDKCINPTFILDHPQIMSPLSKYHRDI 522
N L C + +V+CPPP+TTARLLDKLVGEFLE C+NPTFI++ P+IMSPL+K+HR
Sbjct: 454 NKYLIDACARFDVKCPPPQTTARLLDKLVGEFLEPTCVNPTFIINQPEIMSPLAKWHRSK 513
Query: 523 PG 528
G
Sbjct: 514 SG 515
Score = 81.4 bits (192), Expect = 3e-16
Identities = 35/48 (72%), Positives = 41/48 (85%)
Frame = +2
Query: 2 LVVGGLDRVMKSEDSSRNEGIDLTHNPEFTTCEFYMAYADYNDLITIT 145
L+VGGL+RV + RNEGIDLTHNPEFTTCEFYMA+ADYNDL+ +T
Sbjct: 343 LIVGGLERVYEIGKQFRNEGIDLTHNPEFTTCEFYMAFADYNDLMEMT 390
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 52.8 bits (121), Expect = 1e-07
Identities = 22/45 (48%), Positives = 31/45 (68%)
Frame = +2
Query: 2 LVVGGLDRVMKSEDSSRNEGIDLTHNPEFTTCEFYMAYADYNDLI 136
++VGG ++V + RNEGI HNPEFTT E Y AY+DY+ ++
Sbjct: 315 MLVGGFEKVYEIGRIFRNEGISTRHNPEFTTIEMYEAYSDYHSMM 359
Score = 38.3 bits (85), Expect = 0.003
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +1
Query: 412 LLDKLVGEFLEDKCINPTFILDHPQIMSPLSKYHRDIPG 528
LL+++ +E K + PTF+LD+P +SPL+K HR G
Sbjct: 448 LLNEIFEVVVEPKLVQPTFVLDYPIEISPLAKPHRGNAG 486
>At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N)
family protein similar to SP|P36419 Aspartyl-tRNA
synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) {Thermus thermophilus}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 664
Score = 29.5 bits (63), Expect = 1.5
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 2 LVVGGLDRVMKSEDSSRNEGIDLTHNPEFTTCEFYMAYADYNDLITI 142
L+V G D+ + R+E + PEFT + MA+ D++ +
Sbjct: 283 LMVSGFDKYYQIARCFRDEDLRADRQPEFTQLDMEMAFMPMEDMLKL 329
>At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 822
Score = 28.3 bits (60), Expect = 3.4
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = +1
Query: 334 AESNALLSQLCEKHEVECPPPRTTARLLDKLVGEFLEDKCINPTF 468
+ SN ++ LC+ C P R T + L G DK +N F
Sbjct: 435 SRSNEMIKMLCDMKSNGCSPNRATWNTMLALCGNKGMDKFVNRVF 479
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 27.9 bits (59), Expect = 4.5
Identities = 16/37 (43%), Positives = 18/37 (48%)
Frame = -2
Query: 473 RINVGLMHLSSRNSPTNLSRSRAVVLGGGHSTSCFSH 363
RI L HL+S + NLS A GG S FSH
Sbjct: 15 RIGALLRHLNSGSDSDNLSSLYASPTSGGTGGSVFSH 51
>At5g01190.1 68418.m00024 laccase, putative / diphenol oxidase,
putative similar to diphenol oxidase [Nicotiana
tabacum][GI:1685087]
Length = 553
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 3/34 (8%)
Frame = +2
Query: 431 GNFLKISASIQHLFWIIHR---L*AHYLNTTGIY 523
GNF ++ A+I ++ + + + L AHY N TGIY
Sbjct: 367 GNFSRVVAAINNITFKMPKTALLQAHYFNLTGIY 400
>At1g07510.1 68414.m00804 FtsH protease, putative similar to
AAA-metalloprotease FtsH [Pisum sativum] GI:15021761;
contains Pfam profiles PF01434: Peptidase family M41,
PF00004: ATPase AAA family
Length = 813
Score = 27.9 bits (59), Expect = 4.5
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = -2
Query: 419 SRSRAVVLGGGHSTSCFSHNWLR--RAFDSAVSSVSGEGNFTFKIFSKLA 276
SRS+ V GGG ++ F+ LR + ++AV+ V G F + F+ A
Sbjct: 14 SRSKGFVYGGGVRSAVFNQGRLRAPQNLEAAVNQVDGGLGFLRRHFASFA 63
>At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 788
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2
Query: 320 IH*IQQNQMLSSASCVRNTRWNAHRQGLQ 406
+H IQ+ L A C R RW+ H L+
Sbjct: 436 VHSIQETGYLELACCFRKGRWSTHYTDLE 464
>At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 558
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2
Query: 320 IH*IQQNQMLSSASCVRNTRWNAHRQGLQ 406
+H IQ+ L A C R RW+ H L+
Sbjct: 440 VHSIQETGYLELACCFRKGRWSTHYTDLE 468
>At3g27880.1 68416.m03476 expressed protein
Length = 242
Score = 27.1 bits (57), Expect = 7.8
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = -2
Query: 344 FDSAVSSVSGEGNFTFKIFSKLAIIGTLA 258
F +A +S SG+G F +F+K I G ++
Sbjct: 41 FSTAATSSSGDGGLVFPVFNKNLISGDVS 69
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,104,146
Number of Sequences: 28952
Number of extensions: 251119
Number of successful extensions: 637
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 636
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -