Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_F15
(612 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 43 4e-05
SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein s... 27 2.8
SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3 |Schizosac... 26 4.9
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 6.5
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 25 6.5
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 6.5
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 6.5
SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.6
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 8.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 124 bits (298), Expect = 1e-29
Identities = 52/74 (70%), Positives = 63/74 (85%)
Frame = -3
Query: 610 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSSKSGDAAIVNLVP 431
LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK KSGDA I +VP
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
Query: 430 SKPLCVESFQEFPP 389
SKP+CVE+F ++ P
Sbjct: 405 SKPMCVEAFTDYAP 418
Score = 47.2 bits (107), Expect = 2e-06
Identities = 24/36 (66%), Positives = 25/36 (69%)
Frame = -1
Query: 432 PPSPCVWSPSRNSHPLGRFAVRDMRQTVAVGVIKAV 325
P P + PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 404 PSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 124 bits (298), Expect = 1e-29
Identities = 52/74 (70%), Positives = 63/74 (85%)
Frame = -3
Query: 610 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSSKSGDAAIVNLVP 431
LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK KSGDA I +VP
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
Query: 430 SKPLCVESFQEFPP 389
SKP+CVE+F ++ P
Sbjct: 405 SKPMCVEAFTDYAP 418
Score = 47.2 bits (107), Expect = 2e-06
Identities = 24/36 (66%), Positives = 25/36 (69%)
Frame = -1
Query: 432 PPSPCVWSPSRNSHPLGRFAVRDMRQTVAVGVIKAV 325
P P + PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 404 PSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 124 bits (298), Expect = 1e-29
Identities = 52/74 (70%), Positives = 63/74 (85%)
Frame = -3
Query: 610 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSSKSGDAAIVNLVP 431
LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK KSGDA I +VP
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
Query: 430 SKPLCVESFQEFPP 389
SKP+CVE+F ++ P
Sbjct: 405 SKPMCVEAFTDYAP 418
Score = 47.2 bits (107), Expect = 2e-06
Identities = 24/36 (66%), Positives = 25/36 (69%)
Frame = -1
Query: 432 PPSPCVWSPSRNSHPLGRFAVRDMRQTVAVGVIKAV 325
P P + PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 404 PSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 42.7 bits (96), Expect = 4e-05
Identities = 21/72 (29%), Positives = 38/72 (52%)
Frame = -3
Query: 610 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSSKSGDAAIVNLVP 431
L P ++ GY+ V+ HTA FA++ K+D +T + ++ P + G I L
Sbjct: 567 LELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELET 625
Query: 430 SKPLCVESFQEF 395
P+C+E F+++
Sbjct: 626 QTPVCMERFEDY 637
Score = 25.4 bits (53), Expect = 6.5
Identities = 12/23 (52%), Positives = 17/23 (73%), Gaps = 1/23 (4%)
Frame = -1
Query: 387 LGRFAVRDMRQTVAVG-VIKAVN 322
+GRF +RD TVAVG V+K ++
Sbjct: 640 MGRFTLRDQGTTVAVGKVVKILD 662
>SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein
subunit L40|Schizosaccharomyces pombe|chr 1|||Manual
Length = 279
Score = 26.6 bits (56), Expect = 2.8
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 426 SPCVWSPSRNSHPLGRFAVRDMR 358
S WSPS + PL FA+ D+R
Sbjct: 208 SKVTWSPSLSEPPLPPFAIGDLR 230
>SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 328
Score = 25.8 bits (54), Expect = 4.9
Identities = 24/108 (22%), Positives = 42/108 (38%), Gaps = 2/108 (1%)
Frame = -3
Query: 442 NLVPSKPLCVESFQEFPPPRSFRRA*HEADGRRRCNKGCELQGSRWWQSHQSCRKSHQGQ 263
N + K + ++F E F+ EAD + + + G L + S +
Sbjct: 109 NTITRKDIVQKAFPEGTSRNLFQSVFEEADRQLQLSFGFRLVAVTQSNRKKDMAVSQLRR 168
Query: 262 EVASTVNSSVLYTTAILHS--PKGVQKERRATNSFLFYIFYMAYTVTL 125
S NSS L+ +L S P +QK+ R + Y + +T+
Sbjct: 169 PATSNANSSNLHRYWVLRSTLPMELQKDSRLIVDSVLDTAYYGFLMTV 216
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 6.5
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 503 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 604
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 25.4 bits (53), Expect = 6.5
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +3
Query: 366 HARRNDRGGGNSWKDSTH 419
H+RR+ R N+W++ TH
Sbjct: 200 HSRRSYRNDRNNWRERTH 217
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 25.4 bits (53), Expect = 6.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 392 WEFLEGLHTQGLGGNQVDNGGITRFTGFRV 481
++FL+ + + LGGN + G+T G+ +
Sbjct: 389 YDFLQFMTSSPLGGNMACSAGLTSLLGYHL 418
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.4 bits (53), Expect = 6.5
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = -2
Query: 398 IPTPSVVSPCVT*GRRSPSV**RL*TSRKQVVAKSPKLPKKPP 270
+P+PS +SP VT + P V RL V A P++P+ PP
Sbjct: 1040 VPSPSALSPSVT--PQLPPVSSRL----PPVSATRPQIPQPPP 1076
>SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 143
Score = 25.0 bits (52), Expect = 8.6
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = -3
Query: 523 KEKVDRRTGKSTEDNPKSSKSGDAAIVNL 437
+EKV +RT S N +S++ D ++NL
Sbjct: 39 EEKVTQRTASSDSLNTIASENNDENVINL 67
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 8.6
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +2
Query: 266 PLVAFSAALVTLPPPASLKFTAFITPTATVCLMSRTA 376
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,617,385
Number of Sequences: 5004
Number of extensions: 56104
Number of successful extensions: 173
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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