Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_E24
(537 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 109 2e-25
SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase |Schizosacchar... 108 6e-25
SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyce... 66 4e-12
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 51 1e-07
SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces p... 29 0.33
SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 28 0.77
SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomy... 28 0.77
SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein Rga5|Sch... 26 3.1
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 26 4.1
SPBC3B9.03 |||signal recognition particle receptor alpha subunit... 25 5.4
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 25 7.2
SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces ... 25 7.2
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 25 7.2
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 25 9.5
>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 429
Score = 109 bits (263), Expect = 2e-25
Identities = 52/143 (36%), Positives = 91/143 (63%), Gaps = 1/143 (0%)
Frame = +1
Query: 106 SPMDCYLVNRSLKTLAIRMEQHKKSSLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQ- 282
+P +L+ + L+TL++RME+H +++ +A +L KHPKV V +PGL SH H++ KK
Sbjct: 279 NPFGVFLLLQGLETLSLRMERHVQNAFALAKYLEKHPKVNWVSYPGLESHVSHKLAKKYL 338
Query: 283 TSGHSGVFSFVHVGGLKESNKFLSSLKIFTLAESLGGYESLAELPSVMTHASVPAEQRAQ 462
+G+ V SF GG +S K +++LK+ + ++G ++L P+ TH + E++
Sbjct: 339 KNGYGAVLSFGAKGGPDQSRKVVNALKLASQLANVGDAKTLVIAPAYTTHLQLTDEEQIS 398
Query: 463 LGITDSLVRLSVGLEDTEDLIAD 531
G+T L+R++VG+E +D+IAD
Sbjct: 399 AGVTKDLIRVAVGIEHIDDIIAD 421
>SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 108 bits (259), Expect = 6e-25
Identities = 61/180 (33%), Positives = 101/180 (56%), Gaps = 1/180 (0%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
Y++GH D++ G IA + F NAMG +P +C+L+ R +KT+ +R+E+ +++
Sbjct: 203 YLSGHHDLMGGVIATKSDEIAKSVFFNINAMGAAMAPFECFLLLRGIKTMGLRVERAQQN 262
Query: 181 SLIIANWLLKHPKVKDVLHPGL-PSHPQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSS 357
++ IA +L K V PGL P I G V S V G ++ S +++
Sbjct: 263 AIEIAKFLKS--KGLQVNFPGLDPDAKSTAIFYSFARGPGAVMS-VFTGDVEVSKTIVNT 319
Query: 358 LKIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLEDTEDLIADLE 537
K+F ++ S G SL +P+ M+HAS+ E R G+++ L+R+ VG+E+ +DL ADLE
Sbjct: 320 TKLFEISVSFGAVNSLISMPAYMSHASIKKEVRDARGLSEDLIRICVGIENVDDLKADLE 379
>SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 398
Score = 65.7 bits (153), Expect = 4e-12
Identities = 59/192 (30%), Positives = 86/192 (44%), Gaps = 14/192 (7%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
Y+ GHSDV+ G D L+ + +G + P +L+ RSL+T +R+ +H ++
Sbjct: 213 YLAGHSDVLAGVTASKDRSKILDLKADRAYLGTILHPQQAFLLLRSLRTFPLRIAKHSEN 272
Query: 181 SLIIANWLLK--------------HPKVKDVLHPGLPSHPQHEITKKQTSGHSGVFSFVH 318
++A L K + +V H L + + + K T GH+ FS V
Sbjct: 273 GFLVAQHLNKLATDEQFATSLGIDSSLILEVYHNSLQT--KEFVAKNLTGGHASCFS-VL 329
Query: 319 VGGLKESNKFLSSLKIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSV 498
+ + LK F A SLG ESL E MT + I LVRLS+
Sbjct: 330 LKSDTVAKHLCCELKYFHHATSLGSVESLIEWRR-MTDSK----------IDPRLVRLSI 378
Query: 499 GLEDTEDLIADL 534
G+ED DLIADL
Sbjct: 379 GIEDAADLIADL 390
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 51.2 bits (117), Expect = 1e-07
Identities = 35/137 (25%), Positives = 70/137 (51%), Gaps = 1/137 (0%)
Frame = +1
Query: 115 DCYLVNRSLKTLAIRMEQHKKSSLIIANWLLKHPKVKDVLHPGLPSHPQH-EITKKQTSG 291
D + R+ + A R + ++ I N L ++PK+K + +P + +H E +++ G
Sbjct: 468 DALTLERNSRDFAERSQVINHNAETICNLLYQNPKIKTLYYPKYNTSKEHFEACRRENGG 527
Query: 292 HSGVFSFVHVGGLKESNKFLSSLKIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGI 471
+ G+ S V +++ +F +++ SLG +LA +++ H + + GI
Sbjct: 528 YGGLLSVVFHNP-EDARQFYDKIQV-AKGPSLGTNFTLASPYAILAHYQ-ELDWAGENGI 584
Query: 472 TDSLVRLSVGLEDTEDL 522
+LVR+SVG+E +E L
Sbjct: 585 DRNLVRVSVGMEPSEHL 601
>SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 598
Score = 29.5 bits (63), Expect = 0.33
Identities = 18/61 (29%), Positives = 30/61 (49%)
Frame = +1
Query: 289 GHSGVFSFVHVGGLKESNKFLSSLKIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLG 468
G V ++H GL + + + K TLAE+L G++ LAE ++ S P + L
Sbjct: 360 GIPSVLKYLHAEGLIDGSNITVTGK--TLAENLRGFKDLAEGQKIIRPLSNPIKTEGHLR 417
Query: 469 I 471
+
Sbjct: 418 V 418
>SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 73
Score = 28.3 bits (60), Expect = 0.77
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = +2
Query: 167 NIRNPHSLSRI-GYSNILKLKMFCIQVFRL 253
N NPH L +I YS L +C+ ++RL
Sbjct: 4 NHENPHFLFKIDSYSKTFLLSCYCVSIYRL 33
>SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 505
Score = 28.3 bits (60), Expect = 0.77
Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Frame = +1
Query: 184 LIIANWLLKHPKVKDVLHPGLPSHPQHE----ITKKQTSGHSGVFSFVHVGGLKESNKFL 351
L++ WL+ P D + L P E IT + + + + + VGG E +L
Sbjct: 120 LVVDFWLI--PTEGDPVSASLSLPPWKEAVRIITPITSLSANIILNMLPVGGFGEIEYWL 177
Query: 352 SSLKIFTLA 378
SS+K+FT+A
Sbjct: 178 SSIKVFTVA 186
>SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein
Rga5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 361
Score = 26.2 bits (55), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = +1
Query: 229 VLHPGLPSHPQHEITKK--QTSGHSGVFSFVHVG 324
+ PG+ SHP HE+ K Q S + +F H G
Sbjct: 209 IFQPGILSHPDHEVYSKDYQISQTALLFLINHQG 242
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 25.8 bits (54), Expect = 4.1
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 4/56 (7%)
Frame = -2
Query: 524 IRSSVSSNPTESL--TRESVIPS*ALCSAGTDAC-VXTDGNSAKLSY-PPKLSASV 369
I S + P E T + ++P +C + +D+ V + NSAKL Y PP L++ +
Sbjct: 731 IGSRFYAQPEEEFQTTMDDILPC-YICESSSDSLSVISRSNSAKLPYVPPSLTSLI 785
>SPBC3B9.03 |||signal recognition particle receptor alpha subunit
Srp101|Schizosaccharomyces pombe|chr 2|||Manual
Length = 547
Score = 25.4 bits (53), Expect = 5.4
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = +1
Query: 319 VGGLKESNKFLSSLKIFTLAESLGGYESLAE 411
+G L++ K KIF +AE+L G +SLA+
Sbjct: 449 MGSLEKFTKATKLDKIFQVAEALVGTDSLAQ 479
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 25.0 bits (52), Expect = 7.2
Identities = 26/88 (29%), Positives = 41/88 (46%), Gaps = 9/88 (10%)
Frame = -2
Query: 530 SAIRSSVSSNPTESLTRESVIPS*ALCSAGTDACVXTDGN----SAKLSYPPKL-----S 378
S++++S SSN + LT++S P+ A V T N + + P + S
Sbjct: 655 SSLQTS-SSNKSSLLTKKSSFPALLKGFLALPATVTTSANPLGFKSTIQRPIPIKNNEDS 713
Query: 377 ASVKIFNELKNLFDSFKPPTCTKLNTPE 294
S K F++ + KPPT K N+PE
Sbjct: 714 DSCKAFSQAPPIATK-KPPTFLKPNSPE 740
>SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 615
Score = 25.0 bits (52), Expect = 7.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 130 NRSLKTLAIRMEQHKKSSLIIANWLLKHPKVKDVLH 237
NR ++ + +E H SL + N + +VK+VLH
Sbjct: 522 NREIQNEPVSLESHFSKSLALQNDPTEVIQVKNVLH 557
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.0 bits (52), Expect = 7.2
Identities = 17/45 (37%), Positives = 19/45 (42%)
Frame = -3
Query: 457 PFARRVPTRASXPTVIPPNSRILPNFQPV*RFSTNLKTYSTLLNH 323
PFA P S T PPNS N P FS+ + T NH
Sbjct: 224 PFATSSPNFLSTSTPAPPNSN---NANPSTLFSSIPSSRHTTSNH 265
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +1
Query: 223 KDVLHPGLPSHPQHEITKKQTSGHS 297
+D L LPS HEITK Q G +
Sbjct: 88 EDPLRKRLPSLAIHEITKAQLDGEN 112
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.133 0.378
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,135,001
Number of Sequences: 5004
Number of extensions: 40810
Number of successful extensions: 143
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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