Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_E24
(537 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /... 157 6e-39
At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /... 157 6e-39
At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloropla... 151 2e-37
At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla... 128 3e-30
At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /... 83 1e-16
At1g64660.1 68414.m07330 Cys/Met metabolism pyridoxal-phosphate-... 65 3e-11
At2g02490.1 68415.m00188 hydroxyproline-rich glycoprotein family... 31 0.64
At3g24380.1 68416.m03061 hypothetical protein similar to At5g368... 30 0.85
At1g27790.1 68414.m03401 hypothetical protein similar to At3g243... 30 0.85
At4g05290.1 68417.m00801 hypothetical protein similar to At3g243... 30 1.1
At2g14774.1 68415.m01671 hypothetical protein 29 1.5
At4g07943.1 68417.m01271 hypothetical protein similar to At4g052... 29 2.6
At3g43390.1 68416.m04592 hypothetical protein similar to At3g243... 29 2.6
At2g02800.2 68415.m00225 protein kinase (APK2b) identical to pro... 29 2.6
At2g02800.1 68415.m00224 protein kinase (APK2b) identical to pro... 29 2.6
At2g45620.1 68415.m05672 nucleotidyltransferase family protein l... 28 4.5
At2g05564.1 68415.m00590 hypothetical protein 28 4.5
At1g54920.2 68414.m06269 expressed protein 28 4.5
At2g31650.1 68415.m03864 trithorax 1 (ATX-1) (TRX1) identical to... 27 7.9
>At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 449
Score = 157 bits (380), Expect = 6e-39
Identities = 75/179 (41%), Positives = 114/179 (63%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
++ GHSDV+ G V +A ++ FLQN+ G +P DC+L R +KT+A+R+E+ +++
Sbjct: 264 FIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAPFDCWLCLRGIKTMALRIEKQQEN 323
Query: 181 SLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSSL 360
+ IA +L HP+VK V + GLP HP H + Q G VFSF+ G + S + +
Sbjct: 324 ARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAKGAGSVFSFI-TGSVALSKHLVETT 382
Query: 361 KIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLEDTEDLIADLE 537
K F++A S G +SL +P M+HAS+PAE R G+T+ LVR+S G+ED +DLI+DL+
Sbjct: 383 KYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAGIEDVDDLISDLD 441
>At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 464
Score = 157 bits (380), Expect = 6e-39
Identities = 75/179 (41%), Positives = 114/179 (63%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
++ GHSDV+ G V +A ++ FLQN+ G +P DC+L R +KT+A+R+E+ +++
Sbjct: 279 FIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAPFDCWLCLRGIKTMALRIEKQQEN 338
Query: 181 SLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSSL 360
+ IA +L HP+VK V + GLP HP H + Q G VFSF+ G + S + +
Sbjct: 339 ARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAKGAGSVFSFI-TGSVALSKHLVETT 397
Query: 361 KIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLEDTEDLIADLE 537
K F++A S G +SL +P M+HAS+PAE R G+T+ LVR+S G+ED +DLI+DL+
Sbjct: 398 KYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAGIEDVDDLISDLD 456
>At3g01120.1 68416.m00016 cystathionine gamma-synthase, chloroplast
/ O-succinylhomoserine (Thiol)-lyase (CGS) identical to
SP|P55217 Cystathionine gamma-synthase, chloroplast
precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine
(Thiol)-lyase) {Arabidopsis thaliana}
Length = 563
Score = 151 bits (367), Expect = 2e-37
Identities = 74/178 (41%), Positives = 115/178 (64%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
Y+ GH+DV+ G I + +++R L + +G +P YL+ R +KTL +R++Q +
Sbjct: 380 YIGGHNDVLAGC-ICGSLKLVSEIRNLHHVLGGTLNPNAAYLIIRGMKTLHLRVQQQNST 438
Query: 181 SLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSSL 360
+ +A L HPKV V +PGLPSHP+HE+ K+Q +G GV SF G ++ + KF+ SL
Sbjct: 439 AFRMAEILEAHPKVSHVYYPGLPSHPEHELAKRQMTGFGGVVSFEIDGDIETTIKFVDSL 498
Query: 361 KIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLEDTEDLIADL 534
KI +A S GG ES+ + P++M++ +P E+R + GI D+LVR S G+ED ED+ AD+
Sbjct: 499 KIPYIAPSFGGCESIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFGVEDFEDVKADI 556
>At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast,
putative / O-succinylhomoserine (Thiol)-lyase, putative
strong similarity to SP|P55217 Cystathionine
gamma-synthase, chloroplast precursor (EC 4.2.99.9)
(CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis
thaliana}; contains Pfam profile PF01053: Cys/Met
metabolism PLP-dependent enzyme
Length = 412
Score = 128 bits (308), Expect = 3e-30
Identities = 71/178 (39%), Positives = 105/178 (58%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
Y+ GH+D + G+ I + +K+R L +G +P YL+ R +KT+ +R+ Q +
Sbjct: 230 YIGGHNDFLAGS-ISGSMELVSKIRNLHKLLGGTLNPNAAYLLIRGMKTMHLRVRQQNST 288
Query: 181 SLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSSL 360
+ +A L HPKV V + GLPSHP+H I K+Q +G G+ SF G LK + KF+ +L
Sbjct: 289 GMKMAQVLEAHPKVSRVYYLGLPSHPEHLIAKRQMTGIGGLISFEIDGDLKTTIKFIDAL 348
Query: 361 KIFTLAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLEDTEDLIADL 534
KI LA S GG ESL + + +P E+R + G D+LVR S G+ED ED+ AD+
Sbjct: 349 KIPYLAASFGGCESLVDQLATGIW-DIPREERLKDGFQDNLVRFSFGIEDFEDIKADV 405
>At3g57050.3 68416.m06352 cystathionine beta-lyase, chloroplast /
beta-cystathionase / cysteine lyase (CBL) identical to
SP|P53780 Cystathionine beta-lyase, chloroplast
precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
(Cysteine lyase) {Arabidopsis thaliana}
Length = 378
Score = 83.0 bits (196), Expect = 1e-16
Identities = 37/90 (41%), Positives = 59/90 (65%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDCYLVNRSLKTLAIRMEQHKKS 180
++ GHSDV+ G V +A ++ FLQN+ G +P DC+L R +KT+A+R+E+ +++
Sbjct: 279 FIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAPFDCWLCLRGIKTMALRIEKQQEN 338
Query: 181 SLIIANWLLKHPKVKDVLHPGLPSHPQHEI 270
+ IA +L HP+VK V + GLP HP H +
Sbjct: 339 ARKIAMYLSSHPRVKKVYYAGLPDHPGHHL 368
>At1g64660.1 68414.m07330 Cys/Met metabolism
pyridoxal-phosphate-dependent enzyme family protein
similar to SP|P13254 Methionine gamma-lyase (EC
4.4.1.11) (L-methioninase) {Pseudomonas putida};
contains Pfam profile PF01053: Cys/Met metabolism
PLP-dependent enzyme
Length = 441
Score = 64.9 bits (151), Expect = 3e-11
Identities = 46/181 (25%), Positives = 95/181 (52%), Gaps = 10/181 (5%)
Frame = +1
Query: 1 YMNGHSDVIMGAAIVNDGVIAAKLRFLQNAMGIVPSPMDC---YLVNRSLKTLAIRMEQH 171
+++G +D+I GA ++ ++ + ++ ++ M+ + ++ + L +RM +H
Sbjct: 249 FISGGADIIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREH 308
Query: 172 KKSSLIIANWLLKHPKVKDVLHPGLPSHPQHEITKKQTS---GHSGVFSFVHVGGLKESN 342
+ + A ++ +K V++PGL +HPQH++ K + G+ G+ S + + +++N
Sbjct: 309 SHRAQVYAE-RMRDLGMK-VIYPGLETHPQHKLFKGMVNRDYGYGGLLS-IDMETEEKAN 365
Query: 343 KFLSSLKIFT----LAESLGGYESLAELPSVMTHASVPAEQRAQLGITDSLVRLSVGLED 510
K ++ L+ T +A SLG YE+L T + + Q+ GI+ LVR+SVG
Sbjct: 366 KLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVG 425
Query: 511 T 513
T
Sbjct: 426 T 426
>At2g02490.1 68415.m00188 hydroxyproline-rich glycoprotein family
protein related to LENOD2 [Lupinus luteus]
gi|296830|emb|CAA39050; and genefinder
Length = 302
Score = 30.7 bits (66), Expect = 0.64
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = +1
Query: 208 KHPKVKDVLHPGLPS--HPQHEITKKQTSGHSGVFSFVH 318
++P V + HP PS HP H+ TK + + GVF H
Sbjct: 72 QYPSVFPIPHPVPPSPGHPPHQNTKISVNQYPGVFPIPH 110
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = +1
Query: 208 KHPKVKDVLHPGLPS--HPQHEITKKQTSGHSGVFSFVH 318
++P V + HP PS HP H+ K + + GVF H
Sbjct: 101 QYPGVFPIPHPVPPSPGHPPHQNAKISVNQYPGVFPIPH 139
>At3g24380.1 68416.m03061 hypothetical protein similar to At5g36840,
At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
At2g05560, At4g08880, At1g34730, At1g27790
Length = 230
Score = 30.3 bits (65), Expect = 0.85
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C ++TPEC VC+
Sbjct: 173 KYGNTLKYTYANSFSFPECVDVSTPECAEVCY 204
>At1g27790.1 68414.m03401 hypothetical protein similar to At3g24380,
At5g36840, At5g35010, At3g42740, At4g05290, At2g14770,
At3g43390, At2g05560, At4g08880, At1g34730
Length = 120
Score = 30.3 bits (65), Expect = 0.85
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C ++TPEC VC+
Sbjct: 63 KYGNTLKYTYANSFSFPECVDVSTPECAEVCY 94
>At4g05290.1 68417.m00801 hypothetical protein similar to At3g24380,
At5g36840, At5g35010, At3g42740, At2g14770, At3g43390,
At2g05560, At4g08880, At1g34730, At1g27790
Length = 213
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C L+TPEC +C+
Sbjct: 156 KYGNTLKYTYANSFSYPECGDLSTPECAEMCY 187
>At2g14774.1 68415.m01671 hypothetical protein
Length = 230
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C ++TPEC VC+
Sbjct: 173 KYGNTLKYTYANSFSYPECGDVSTPECADVCY 204
>At4g07943.1 68417.m01271 hypothetical protein similar to At4g05290,
At3g42740, At5g35010, At5g36840, At3g43390, At2g14770,
At2g05560, At4g08880, At1g34730
Length = 213
Score = 28.7 bits (61), Expect = 2.6
Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + SF P C + TP+C ++C+
Sbjct: 156 KYGNTLKYTYAKSFSYPECGDIRTPDCAMMCY 187
>At3g43390.1 68416.m04592 hypothetical protein similar to At3g24380,
At5g36840, At5g35010, At3g42740, At4g05290, At2g14770,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At3g24390,
At4g05280, At1g25886, At4g03300
Length = 1113
Score = 28.7 bits (61), Expect = 2.6
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C ++TPEC +C+
Sbjct: 1056 KYGNTLKYTYANSFSYPKCGDVSTPECTEMCY 1087
>At2g02800.2 68415.m00225 protein kinase (APK2b) identical to
protein kinase APK2b [Arabidopsis thaliana]
gi|2852449|dbj|BAA24695
Length = 426
Score = 28.7 bits (61), Expect = 2.6
Identities = 21/71 (29%), Positives = 34/71 (47%)
Frame = -2
Query: 536 SKSAIRSSVSSNPTESLTRESVIPS*ALCSAGTDACVXTDGNSAKLSYPPKLSASVKIFN 357
S A +S+SS + +R +V S ++ S + + T ++ P L A FN
Sbjct: 17 SPHANSASLSSRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEGEILSSPNLKAFT--FN 74
Query: 356 ELKNLFDSFKP 324
ELKN +F+P
Sbjct: 75 ELKNATRNFRP 85
>At2g02800.1 68415.m00224 protein kinase (APK2b) identical to
protein kinase APK2b [Arabidopsis thaliana]
gi|2852449|dbj|BAA24695
Length = 426
Score = 28.7 bits (61), Expect = 2.6
Identities = 21/71 (29%), Positives = 34/71 (47%)
Frame = -2
Query: 536 SKSAIRSSVSSNPTESLTRESVIPS*ALCSAGTDACVXTDGNSAKLSYPPKLSASVKIFN 357
S A +S+SS + +R +V S ++ S + + T ++ P L A FN
Sbjct: 17 SPHANSASLSSRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEGEILSSPNLKAFT--FN 74
Query: 356 ELKNLFDSFKP 324
ELKN +F+P
Sbjct: 75 ELKNATRNFRP 85
>At2g45620.1 68415.m05672 nucleotidyltransferase family protein low
similarity to SP|O13833| Caffeine-induced death protein
1 {Schizosaccharomyces pombe}; contains Pfam profiles
PF03828: PAP/25A associated domain, PF01909:
Nucleotidyltransferase domain
Length = 764
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +1
Query: 202 LLKHPKV---KDVLHPGLPSHPQHEITKKQTSGHSGVFSFVHVG 324
L++HP+ + P L H H++ +Q GHSG ++ H+G
Sbjct: 186 LMRHPQSTLSNSNMDPNLSHHRNHDL-HEQRGGHSGRGNWGHIG 228
>At2g05564.1 68415.m00590 hypothetical protein
Length = 231
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -2
Query: 368 KIFNELKNLF-DSFKPPTCTKLNTPECPLVCF 276
K N LK + +SF P C ++TPEC +C+
Sbjct: 174 KYGNTLKYTYANSFLYPECGDVSTPECAEMCY 205
>At1g54920.2 68414.m06269 expressed protein
Length = 890
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = +1
Query: 256 PQHEITKKQTSGHSGVFSFVHVGGLKESNKFLSSLK 363
P+ ++T K+++G F F V L+ +N F SS++
Sbjct: 296 PKRDLTNKESNGWGFGFGFEPVSKLETTNSFQSSVE 331
>At2g31650.1 68415.m03864 trithorax 1 (ATX-1) (TRX1) identical to
trithorax-like protein 1 GI:12659210 from [Arabidopsis
thaliana]; characterized in Alvarez-Venegas R,et al,
ATX-1, an Arabidopsis Homolog of Trithorax, Activates
Flower Homeotic Genes.(Curr Biol. 2003 Apr
15;13(8):627-37 PMID: 12699618); contains Pfam profiles
PF00856: SET domain, PF00855: PWWP domain, PF00628,
PHD-finger; identical to cDNA trithorax-like protein 1
(TRX1) GI:12659209
Length = 1062
Score = 27.1 bits (57), Expect = 7.9
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -2
Query: 455 LCSAGTDACVXTDGNSAKLSYPPKLSASVKIFNELKN 345
+C AC+ NS +++Y P + + + EL+N
Sbjct: 726 ICGVSYGACIQCSNNSCRVAYHPLCARAAGLCVELEN 762
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.133 0.378
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,129,701
Number of Sequences: 28952
Number of extensions: 214955
Number of successful extensions: 633
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 629
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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