Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_E14
(556 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 46 2e-05
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 42 2e-04
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 41 6e-04
At1g64030.1 68414.m07252 serpin family protein / serine protease... 38 0.005
At2g14540.1 68415.m01628 serpin family protein / serine protease... 37 0.010
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 36 0.018
At2g35580.1 68415.m04357 serpin family protein / serine protease... 36 0.018
At5g19600.1 68418.m02333 sulfate transporter, putative similar t... 29 1.6
At4g00510.1 68417.m00070 cyclic nucleotide-binding domain-contai... 29 2.1
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 28 3.6
At4g19920.1 68417.m02918 disease resistance protein (TIR class),... 28 3.6
At1g62170.1 68414.m07013 serpin family protein / serine protease... 28 3.6
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 28 4.8
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 28 4.8
At4g10660.1 68417.m01741 hypothetical protein 27 8.4
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 46.0 bits (104), Expect = 2e-05
Identities = 45/153 (29%), Positives = 65/153 (42%), Gaps = 9/153 (5%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKVKLEMPK----FEIKSKLDL 391
F M + LP+ DGL+++L+K + E G L+ L T L +PK FE K+ L
Sbjct: 234 FSMYIYLPNDKDGLAALLEKISTEPGFLDSHIPLHRTPVDA-LRIPKLNFSFEFKASEVL 292
Query: 390 KP--LMPKFGVSKIFNEPAPNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYS 217
K L F E + GD + VS +A I+VDEE +P
Sbjct: 293 KDMGLTSPFTSKGNLTEMVDSPSNGDKLHVSSIIHKACIEVDEEGTEAAAVSVAIMMPQC 352
Query: 216 SHSRPRQPLVFKVDHPFAYFILYEDK--IIFAG 124
P F DHPF + + ++ I+F G
Sbjct: 353 LMRNPD----FVADHPFLFTVREDNSGVILFIG 381
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 42.3 bits (95), Expect = 2e-04
Identities = 41/154 (26%), Positives = 64/154 (41%), Gaps = 10/154 (6%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDKAG-EKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLM 379
F M + LP+ +GL+ +L+K G E ++ L + +PKF+ + + ++
Sbjct: 169 FSMYIYLPNDKEGLAPLLEKIGSEPSFFDNHIPLHCISVGA-FRIPKFKFSFEFNASEVL 227
Query: 378 PKFGVSKIFN------EPAPNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYS 217
G++ FN E + GD + VS +A I+VDEE S
Sbjct: 228 KDMGLTSPFNNGGGLTEMVDSPSNGDDLYVSSILHKACIEVDEEGTEAAAVSVGVVSCTS 287
Query: 216 SHSRPRQPLVFKVDHPFAYFILYEDK---IIFAG 124
P F D PF F + EDK I+F G
Sbjct: 288 FRRNPD----FVADRPF-LFTVREDKSGVILFMG 316
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 40.7 bits (91), Expect = 6e-04
Identities = 36/137 (26%), Positives = 59/137 (43%), Gaps = 7/137 (5%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLM 379
F M LPD +GLS +LDK G L++ R + + ++PKF+ D ++
Sbjct: 234 FSMYFYLPDANNGLSDLLDKIVSTPGFLDNHIPRRQVKVR-EFKIPKFKFSFGFDASNVL 292
Query: 378 PKFGVSKIFN--EPAPNIVK----GDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYS 217
G++ F+ E +V+ G ++ VS F +A I+V+EE +
Sbjct: 293 KGLGLTSPFSGEEGLTEMVESPEMGKNLCVSNIFHKACIEVNEE-GTEAAAASAGVIKLR 351
Query: 216 SHSRPRQPLVFKVDHPF 166
+ F DHPF
Sbjct: 352 GLLMEEDEIDFVADHPF 368
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 37.9 bits (84), Expect = 0.005
Identities = 39/151 (25%), Positives = 63/151 (41%), Gaps = 7/151 (4%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKV--KLEMPKFEIKSKLDLKP 385
F M LPDK DGL +L+K A G L+ PT R K +PKF+I+ +
Sbjct: 237 FSMYFYLPDKKDGLDDLLEKMASTPGFLDSHI---PTYRDELEKFRIPKFKIEFGFSVTS 293
Query: 384 LMPKFGVSKIFNEPAPNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYS-SHS 208
++ + G+ + + +A +++DEE S
Sbjct: 294 VLDRLGLRSM-----------------SMYHKACVEIDEEGAEAAAATADGDCGCSLDFV 336
Query: 207 RPRQPLVFKVDHPFAYFILYEDK---IIFAG 124
P + + F DHPF F++ E+K ++F G
Sbjct: 337 EPPKKIDFVADHPF-LFLIREEKTGTVLFVG 366
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 36.7 bits (81), Expect = 0.010
Identities = 28/89 (31%), Positives = 43/89 (48%), Gaps = 3/89 (3%)
Frame = -2
Query: 381 MPKFGVSKIFNEPAPNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYSSHSRP 202
+PKF + F A ++ + VS Q+A I++DEE V S P
Sbjct: 308 IPKFKIE--FGFEASSVFNDFELNVS-LHQKALIEIDEEGTEAAAATTVVVVTGSCLWEP 364
Query: 201 RQPLVFKVDHPFAYFILYEDK---IIFAG 124
++ + F DHPF F++ EDK ++FAG
Sbjct: 365 KKKIDFVADHPF-LFLIREDKTGTLLFAG 392
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 35.9 bits (79), Expect = 0.018
Identities = 38/160 (23%), Positives = 65/160 (40%), Gaps = 16/160 (10%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMP 376
F M + LP+ DGL ++L++ K D R ++PKF+ + ++
Sbjct: 234 FAMYIYLPNDRDGLPTLLEEISSKPRFLDNHIPRQRILTEAFKIPKFKFSFEFKASDVLK 293
Query: 375 KFGVSKIFNEPA-----------PNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXX 229
+ G++ F + N+ +++ VS F +A I+VDEE
Sbjct: 294 EMGLTLPFTHGSLTEMVESPSIPENLCVAENLFVSNVFHKACIEVDEEGTEAAA------ 347
Query: 228 VPYSSHSRPRQPLV---FKVDHPFAYFILYEDK--IIFAG 124
S S + L+ F DHPF + + E I+F G
Sbjct: 348 --VSVASMTKDMLLMGDFVADHPFLFTVREEKSGVILFMG 385
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 35.9 bits (79), Expect = 0.018
Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 2/66 (3%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKVK-LEMPKFEIKSKLDLKPL 382
F M + LPD+ DGL S+L++ A +G L+D L + +K L++P+F+ +
Sbjct: 236 FSMQIYLPDEKDGLPSMLERLASTRGFLKDNEVLPSHSAVIKELKIPRFKFDFAFEASEA 295
Query: 381 MPKFGV 364
+ FG+
Sbjct: 296 LKGFGL 301
>At5g19600.1 68418.m02333 sulfate transporter, putative similar to
sulfate transporter [Arabidopsis thaliana] GI:2285885;
contains Pfam profiles PF00916: Sulfate transporter
family, PF01740: STAS domain; supporting cDNA
gi|14141683|dbj|AB061739.1|
Length = 634
Score = 29.5 bits (63), Expect = 1.6
Identities = 19/58 (32%), Positives = 30/58 (51%)
Frame = -2
Query: 525 LDGLSSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 352
L G+S++ D G + LLE L N K+ + P+FE+ K+ L + K G +F
Sbjct: 557 LSGVSTI-DMTGMETLLEIQRILGSKNIKMVIINPRFEVLEKMMLSHFVEKIGKEYMF 613
>At4g00510.1 68417.m00070 cyclic nucleotide-binding
domain-containing protein contains Pfam profile: PF00027
cyclic nucleotide-binding domain
Length = 175
Score = 29.1 bits (62), Expect = 2.1
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = -2
Query: 516 LSSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKF 370
+ SV D E+ L+ED+ L P + +V P F S L +K L F
Sbjct: 118 IKSVSDSDKERCLVEDILYLEPLDVRVSPNCPSFSSISVLMIKVLTIHF 166
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 28.3 bits (60), Expect = 3.6
Identities = 11/47 (23%), Positives = 23/47 (48%)
Frame = -2
Query: 204 PRQPLVFKVDHPFAYFILYEDKIIFAGTYTH*YKTLLTESLILKQSY 64
P Q +++ P +F++ + I+F G + YK + E + +Y
Sbjct: 468 PAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKEVDDDTY 514
>At4g19920.1 68417.m02918 disease resistance protein (TIR class),
putative domain signature TIR exists, suggestive of a
disease resistance protein.
Length = 274
Score = 28.3 bits (60), Expect = 3.6
Identities = 20/65 (30%), Positives = 31/65 (47%), Gaps = 4/65 (6%)
Frame = +1
Query: 154 NEISERMINFENQRLARSGMR*IWYSNCTSESAGCGSFFIYFDKCLLEG----FRHYYTV 321
NE+ R + +R+ S M + +S+ SES C + + D C+ EG +Y V
Sbjct: 85 NEMKGRDLQNLFKRIENSKMALVIFSDRFSESDWCLNELVKIDDCVKEGKLTVIPVFYRV 144
Query: 322 TLDDV 336
DDV
Sbjct: 145 NTDDV 149
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 28.3 bits (60), Expect = 3.6
Identities = 37/147 (25%), Positives = 61/147 (41%), Gaps = 3/147 (2%)
Frame = -2
Query: 555 FRMLVLLPDKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLM 379
F M + LPDK L +L++ G L+ N + ++++ KF I
Sbjct: 301 FAMYIYLPDKKGELDDLLERMTSTPGFLDS------HNPERRVKVGKFRI---------- 344
Query: 378 PKFGVSKIFNEPAPNIVKGDSVVVSEAFQEAFIKVDEEXXXXXXXXXXXXVPYSSHSRPR 199
PKF + F A + + VS +Q+ I++DE+ Y + +
Sbjct: 345 PKFKIE--FGFEASSAFSDFELDVS-FYQKTLIEIDEKGTEAVTFTAFRSA-YLGCALVK 400
Query: 198 QPLVFKVDHPFAYFILYED--KIIFAG 124
P+ F DHPF + I E ++FAG
Sbjct: 401 -PIDFVADHPFLFLIREEQTGTVLFAG 426
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 27.9 bits (59), Expect = 4.8
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -2
Query: 219 SSHSRPRQPLVFKVDHPFAYFILYEDKIIFAGTYTH*YKTLLTESLILKQSYT 61
S RP Q +F+ P + L++ ++ G + YK +TE ++YT
Sbjct: 464 SLKGRPVQARIFEGKEPPQFVALFQHMVVLKGGLSSGYKNSMTEKGSSGETYT 516
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 27.9 bits (59), Expect = 4.8
Identities = 10/42 (23%), Positives = 23/42 (54%)
Frame = -2
Query: 189 VFKVDHPFAYFILYEDKIIFAGTYTH*YKTLLTESLILKQSY 64
+++ + P +F +++ ++F G + YK LL E + + Y
Sbjct: 470 IYQGNEPSRFFPMFQSLVVFKGGLSRRYKVLLAEKEKIGEEY 511
>At4g10660.1 68417.m01741 hypothetical protein
Length = 317
Score = 27.1 bits (57), Expect = 8.4
Identities = 14/44 (31%), Positives = 29/44 (65%)
Frame = +2
Query: 278 LINASWKASDTTTLSPLTMFGAGSLNIFETPNLGIKGLRSNLLF 409
L++++W+ ++TTL +T+F S+ ET L + G+R+++ F
Sbjct: 228 LVSSNWEEINSTTLDGVTIF--ASMYSSET-RLDVLGMRNSVYF 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,767,026
Number of Sequences: 28952
Number of extensions: 239273
Number of successful extensions: 701
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 679
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 699
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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