BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_D24 (636 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 30 0.32 SPAC23A1.15c |sec20||SNARE Sec20|Schizosaccharomyces pombe|chr 1... 26 4.0 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 26 4.0 SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 26 5.2 SPCC1393.13 |||DUF89 family protein|Schizosaccharomyces pombe|ch... 26 5.2 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 26 5.2 SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|... 26 5.2 SPBC1703.11 |||optic atrophy 3 family protein|Schizosaccharomyce... 25 9.1 >SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 767 Score = 29.9 bits (64), Expect = 0.32 Identities = 26/99 (26%), Positives = 40/99 (40%), Gaps = 6/99 (6%) Frame = +2 Query: 251 NTVLRLHPHVCFNRKYFNILKEIGKNDGGVLEHS---GAMFIDSKINVYEQFK---QDVQ 412 N L+++PH N ++ N K IG+ G + H A F+ S + Q K QD++ Sbjct: 484 NYTLQINPHSGINPEHLNYFKFIGRVIGLAIFHRRFVDAFFVVSFYKMILQKKVTLQDME 543 Query: 413 NTGVSEVYELPWXXXXXXXXXINDFVRSATHEAFDEIVT 529 + L W + D S F E+VT Sbjct: 544 SMDAEYYRSLVWILDNDITGVL-DLTFSVEDNCFGEVVT 581 >SPAC23A1.15c |sec20||SNARE Sec20|Schizosaccharomyces pombe|chr 1|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.0 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +1 Query: 94 SVFEEVIVRLQRWTSDESQSRNGKPFRRIW 183 + EE +V LQ+ S E R+ + FR+IW Sbjct: 6 NALEEKVVELQQSESVEVIKRHFREFRKIW 35 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 26.2 bits (55), Expect = 4.0 Identities = 24/99 (24%), Positives = 42/99 (42%), Gaps = 2/99 (2%) Frame = +2 Query: 59 NAYGKCNDYTAHQFLKRSLYDFNA--GLVTRVNQETESHFVVSGLSAWAILSTLSFGAAE 232 ++Y + Y+AH KR + F+ GL E F++S I + A + Sbjct: 444 DSYAEIMRYSAHDLKKRLMIRFDGEDGL-DYGGLSREFFFLLSHKMFDPIYCLFEYSAVD 502 Query: 233 ETFDEINTVLRLHPHVCFNRKYFNILKEIGKNDGGVLEH 349 N L+++PH N ++ N + IG+ G + H Sbjct: 503 ------NYTLQINPHSSINPEHLNYFRFIGRVIGLAIFH 535 >SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosaccharomyces pombe|chr 1|||Manual Length = 180 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/56 (21%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = +1 Query: 61 CIREVQRLYGSSVFEEVIVRLQRWTSDESQSRNGKPFR-RIWTISMGNSIDAIVWR 225 C+ E + E++IVR + W +S + G + + +++ N + VW+ Sbjct: 54 CVDEATMIDAEPELEDIIVRTKLWNFRQSFTIEGSIYEVGSFKVAIANVLQKSVWK 109 >SPCC1393.13 |||DUF89 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 442 Score = 25.8 bits (54), Expect = 5.2 Identities = 22/100 (22%), Positives = 42/100 (42%), Gaps = 1/100 (1%) Frame = +2 Query: 338 VLEHSG-AMFIDSKINVYEQFKQDVQNTGVSEVYELPWXXXXXXXXXINDFVRSATHEAF 514 VL+++G +F+D Y K ++ T + ++PW ++D + + F Sbjct: 250 VLDNAGFELFVDLLFATY-LLKTEIAETIILHPKDVPWF--------VSDVLVNDIPHLF 300 Query: 515 DEIVTPSELDGVLLVLIDALYFKGHWKGVIPLRKYWNRAH 634 + + + +GV + D F K VI +W AH Sbjct: 301 NSLTSYFSGEGVQKLASDLAEFHAEGKIVIRPNPFWTTAH 340 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.8 bits (54), Expect = 5.2 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Frame = +2 Query: 479 NDFVRSATHEAFDE--IVTPSELDGVLLVLIDALYFKGHWKGV 601 +D ++A E +E + P E D V+L DA++ KG K + Sbjct: 164 SDEKQNAYEEKIEERILANPDESDDVMLAARDAIFSKGQSKRI 206 >SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 570 Score = 25.8 bits (54), Expect = 5.2 Identities = 8/10 (80%), Positives = 9/10 (90%) Frame = -1 Query: 420 PVFWTSCLNC 391 PVFW SCL+C Sbjct: 229 PVFWISCLSC 238 >SPBC1703.11 |||optic atrophy 3 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 218 Score = 25.0 bits (52), Expect = 9.1 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = +2 Query: 137 VTRVNQETESHFVVSGLSAWAILSTLSFGAAEETFDEINTVL 262 + R+N+ E FV+ ST + E+ FDE++ V+ Sbjct: 125 IVRINEIMEKQFVLQKKKNELQSSTEEIDSTEKDFDELHKVI 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,496,772 Number of Sequences: 5004 Number of extensions: 49522 Number of successful extensions: 123 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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