Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_C24
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam... 53 2e-07
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 31 0.46
At5g16690.1 68418.m01954 origin recognition complex subunit 3-re... 30 1.4
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 1.9
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 1.9
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 29 3.2
At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related ... 29 3.2
At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger) fa... 28 4.3
At4g01240.1 68417.m00163 hypothetical protein 27 9.9
At1g40230.1 68414.m04784 hypothetical protein 27 9.9
>At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family
protein similar to iturin A synthetase C [Bacillus
subtilis] GI:16040972; contains Pfam profile PF00501:
AMP-binding enzyme
Length = 1040
Score = 52.8 bits (121), Expect = 2e-07
Identities = 49/143 (34%), Positives = 68/143 (47%), Gaps = 11/143 (7%)
Frame = +3
Query: 189 CLKLWVCSGETLSRDLALKFFQYFGDHDGYKLANFYGTTEVMADITYY---VLETLGQMH 359
CLKL V SGE L + N YG+TEV D TY+ L L +
Sbjct: 224 CLKLVVLSGEPFPVSLWDSLHSLLPETC---FLNLYGSTEVSGDCTYFDCSELPRLLKTE 280
Query: 360 LFLTVPIGVPISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGYVRG---KDPHRFIHN 530
+VPIG ISN V LL + + GE+CV+G L++GY+ + + +HN
Sbjct: 281 EIGSVPIGKSISNCKVVLLGDEDKPYE----GEICVSGLCLSQGYMHSSIESEGYVKLHN 336
Query: 531 ----PHNTHPNFAKL-YRTGDFG 584
H T+ ++L YRTGD+G
Sbjct: 337 NSLCNHLTNDCGSQLYYRTGDYG 359
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 31.5 bits (68), Expect = 0.46
Identities = 28/106 (26%), Positives = 45/106 (42%), Gaps = 1/106 (0%)
Frame = +3
Query: 183 LKCLKLWVCSGETLSRDLALKFFQYFGDHDGYKLANFYGTTEVMADITYYVLETLGQMHL 362
L+ L+ C G L +D+A +F Q F D D + YG TE T G +
Sbjct: 320 LRSLRSLGCGGAPLGKDIAERFKQKFPDVD---IVQGYGLTESSGP----AASTFGPEEM 372
Query: 363 FLTVPIGVPISNNYVYLLDENM-RCVREGEPGEVCVAGFNLARGYV 497
+G N ++D + + G+ GE+ + G + +GYV
Sbjct: 373 VKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYV 418
>At5g16690.1 68418.m01954 origin recognition complex subunit
3-related / ORC3-related low similarity to SP|Q9UBD5
Origin recognition complex subunit 3 (Origin recognition
complex subunit Latheo) {Homo sapiens}
Length = 556
Score = 29.9 bits (64), Expect = 1.4
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +3
Query: 183 LKCLKLWVCSGETLSRDLALKFFQYFGDHDGYKLANFYGTTEVMADITYYVLETLGQM 356
LK + L CSG T+S +AL YF DG L +F T ++ A + ++ LE L M
Sbjct: 304 LKAVFLKPCSGFTVSHKVALFMRSYFLCQDG-TLTSFVRTLKI-ACLQHFSLEPLSIM 359
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 29.5 bits (63), Expect = 1.9
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Frame = +3
Query: 381 GVPISNNYVYLLDENMR-CVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFA 557
G + N + ++D + + +PGE+C+ G + +GY ++NP T
Sbjct: 382 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGY---------LNNPAATAETID 432
Query: 558 K--LYRTGDFGILD 593
K TGD G++D
Sbjct: 433 KDGWLHTGDIGLID 446
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 29.5 bits (63), Expect = 1.9
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Frame = +3
Query: 381 GVPISNNYVYLLDENMR-CVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFA 557
G + N + ++D + + +PGE+C+ G + +GY ++NP T
Sbjct: 382 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGY---------LNNPAATAETID 432
Query: 558 K--LYRTGDFGILD 593
K TGD G++D
Sbjct: 433 KDGWLHTGDIGLID 446
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 28.7 bits (61), Expect = 3.2
Identities = 20/73 (27%), Positives = 29/73 (39%), Gaps = 1/73 (1%)
Frame = +3
Query: 378 IGVPISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFA 557
+G P+ +L+E GEVC+ G N+ +GY +NP F
Sbjct: 337 VGKPVGQEMA-ILNEKGEIQEPNNKGEVCIRGPNVTKGYK---------NNPEANKAGFE 386
Query: 558 -KLYRTGDFGILD 593
+ TGD G D
Sbjct: 387 FGWFHTGDIGYFD 399
>At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related
contains weak similarity to Swiss-Prot:P10366 ATP
phosphoribosyltransferase [Escherichia coli]
Length = 1402
Score = 28.7 bits (61), Expect = 3.2
Identities = 14/56 (25%), Positives = 29/56 (51%)
Frame = -1
Query: 202 HNLRHLSGLNAGSKDRYKRIERSRVGTNTKRRTSCSTKTLTSFCGSLVVSLDKITN 35
++ +H + + SKD++ I+ S ++ KR+ KT S++V +K+ N
Sbjct: 542 YHAKHGRRVKSKSKDKHLAIDNSSSSSSGKRKRGTLVKTTGEGVRSIIVDGEKVLN 597
>At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 1092
Score = 28.3 bits (60), Expect = 4.3
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -3
Query: 566 IQFGKIRVSVMRVMDEAMRIFTAHISSSKIETSHTH 459
I F R++ +R EA R+F +HIS++ I T H
Sbjct: 169 ITFWIWRLAFVRTFGEAQRLFLSHISTTVILTDCLH 204
>At4g01240.1 68417.m00163 hypothetical protein
Length = 659
Score = 27.1 bits (57), Expect = 9.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 3 EIWGPLIHGRSLVILSRDTTRDPQ 74
++W +I G V L RDTT+D Q
Sbjct: 104 KVWETMILGEKAVELERDTTQDAQ 127
>At1g40230.1 68414.m04784 hypothetical protein
Length = 303
Score = 27.1 bits (57), Expect = 9.9
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -2
Query: 474 NQPHTPHRVHLPERIAYSHPEGKRNCSISVHQ 379
N P +P VH PE +++SH + S V +
Sbjct: 224 NSPFSPFVVHPPELLSHSHEHSTAHLSTGVRR 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,892,235
Number of Sequences: 28952
Number of extensions: 287668
Number of successful extensions: 753
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 735
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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