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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_C09
         (371 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, puta...   108   1e-24
At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a)    106   5e-24
At1g45000.1 68414.m05158 26S proteasome regulatory complex subun...    33   0.061
At5g43010.1 68418.m05245 26S proteasome AAA-ATPase subunit (RPT4...    33   0.080
At5g35475.1 68418.m04219 hypothetical protein very low similarit...    27   3.0  
At3g29185.2 68416.m03659 expressed protein                             27   3.0  
At3g29185.1 68416.m03658 expressed protein                             27   3.0  
At4g11730.1 68417.m01871 ATPase, plasma membrane-type, putative ...    26   7.0  

>At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit,
           putative almost identical to 26S proteasome AAA-ATPase
           subunit RPT6a GI:6652888 from [Arabidopsis thaliana];
           almost identical to a member of conserved Sug1 CAD
           family AtSUG1 GI:13537115 from [Arabidopsis thaliana]
          Length = 419

 Score =  108 bits (259), Expect = 1e-24
 Identities = 52/84 (61%), Positives = 62/84 (73%)
 Frame = +2

Query: 110 KGEGFRPYYITKIEELQLIVAEKSQNLRRLQAQRNELNAKVRMLRXXXXXXXXXGSYVGE 289
           +GEG   YY+  ++ELQ +  EKS NL RL+AQRNELN++VRMLR         GSYVGE
Sbjct: 28  QGEGLNKYYLQHLDELQRLQREKSYNLNRLEAQRNELNSRVRMLREELQLLQEPGSYVGE 87

Query: 290 VVKPMDKKKVLVKVHPEGKFVVDL 361
           VVK M K KVLVKVHPEGK+VVD+
Sbjct: 88  VVKVMGKNKVLVKVHPEGKYVVDI 111


>At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a)
          Length = 419

 Score =  106 bits (254), Expect = 5e-24
 Identities = 51/84 (60%), Positives = 62/84 (73%)
 Frame = +2

Query: 110 KGEGFRPYYITKIEELQLIVAEKSQNLRRLQAQRNELNAKVRMLRXXXXXXXXXGSYVGE 289
           +GEG + YY+  I ELQ  + +K+ NL RL+AQRNELN++VRMLR         GSYVGE
Sbjct: 28  QGEGLKQYYLQHIHELQRQLRQKTNNLNRLEAQRNELNSRVRMLREELQLLQEPGSYVGE 87

Query: 290 VVKPMDKKKVLVKVHPEGKFVVDL 361
           VVK M K KVLVKVHPEGK+VVD+
Sbjct: 88  VVKVMGKNKVLVKVHPEGKYVVDI 111


>At1g45000.1 68414.m05158 26S proteasome regulatory complex subunit
           p42D, putative similar to 26S proteasome regulatory
           complex subunit p42D [Drosophila melanogaster]
           gi|6434958|gb|AAF08391
          Length = 399

 Score = 33.1 bits (72), Expect = 0.061
 Identities = 19/74 (25%), Positives = 38/74 (51%)
 Frame = +2

Query: 149 EELQLIVAEKSQNLRRLQAQRNELNAKVRMLRXXXXXXXXXGSYVGEVVKPMDKKKVLVK 328
           +EL+  V    +NLR  + + N+    ++ L+         G  +GEV++P+D ++++VK
Sbjct: 26  KELESRVRTARENLRAAKKEFNKTEDDLKSLQSV-------GQIIGEVLRPLDNERLIVK 78

Query: 329 VHPEGKFVVDLXQK 370
                ++VV    K
Sbjct: 79  ASSGPRYVVGCRSK 92


>At5g43010.1 68418.m05245 26S proteasome AAA-ATPase subunit (RPT4a)
           gb|AAF22524.1
          Length = 399

 Score = 32.7 bits (71), Expect = 0.080
 Identities = 19/74 (25%), Positives = 38/74 (51%)
 Frame = +2

Query: 149 EELQLIVAEKSQNLRRLQAQRNELNAKVRMLRXXXXXXXXXGSYVGEVVKPMDKKKVLVK 328
           +EL+  V    +NLR  + + N+    ++ L+         G  +GEV++P+D ++++VK
Sbjct: 26  KELESRVRTARENLRGAKKEFNKTEDDLKSLQSV-------GQIIGEVLRPLDNERLIVK 78

Query: 329 VHPEGKFVVDLXQK 370
                ++VV    K
Sbjct: 79  ASSGPRYVVGCRSK 92


>At5g35475.1 68418.m04219 hypothetical protein very low similarity
           to transposase [Ipomoea purpurea] GI:4063770
          Length = 136

 Score = 27.5 bits (58), Expect = 3.0
 Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
 Frame = +2

Query: 17  FHFIVNL--NLFTVCETK*LANMTVTKMEVDSTKGEG 121
           FH+++N   N  T      +  M + K++VD T GEG
Sbjct: 57  FHYLINKIQNELTALLANEIKAMIIKKIQVDDTSGEG 93


>At3g29185.2 68416.m03659 expressed protein
          Length = 411

 Score = 27.5 bits (58), Expect = 3.0
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +2

Query: 272 GSYVGEVVKPMDKKKVLVKVHPEGKFVVDL 361
           G  V ++    D+KKV   VH EGK   DL
Sbjct: 333 GQVVQDISSTSDEKKVTTNVHWEGKMSKDL 362


>At3g29185.1 68416.m03658 expressed protein
          Length = 433

 Score = 27.5 bits (58), Expect = 3.0
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +2

Query: 272 GSYVGEVVKPMDKKKVLVKVHPEGKFVVDL 361
           G  V ++    D+KKV   VH EGK   DL
Sbjct: 333 GQVVQDISSTSDEKKVTTNVHWEGKMSKDL 362


>At4g11730.1 68417.m01871 ATPase, plasma membrane-type, putative /
           proton pump, putative similar to plasma membrane-type
           ATPase SP|P20431 and SP|P19456 {Arabidopsis thaliana};
           contains InterPro accession IPR001757: ATPase, E1-E2
           type
          Length = 813

 Score = 26.2 bits (55), Expect = 7.0
 Identities = 15/48 (31%), Positives = 26/48 (54%), Gaps = 4/48 (8%)
 Frame = +3

Query: 168 WLKSLKIFVVYKLNVMN*TLKYVCCVKNSSFYRNRVHM----WVKLSN 299
           WL ++  F  + L++M   ++Y+   K  S + N VH+    + KLSN
Sbjct: 737 WLYNIIFF--FPLDIMKFGIRYILTGKAQSLFDNMVHLVLNSYAKLSN 782


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,027,513
Number of Sequences: 28952
Number of extensions: 116546
Number of successful extensions: 254
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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