Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_C06
(632 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 217 4e-57
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 52 4e-07
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 52 4e-07
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 48 5e-06
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 47 1e-05
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 34 0.090
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 32 0.36
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 32 0.36
At5g53130.1 68418.m06604 cyclic nucleotide-regulated ion channel... 31 0.64
At4g21780.1 68417.m03151 expressed protein 31 0.64
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 30 1.5
At1g01340.1 68414.m00049 cyclic nucleotide-regulated ion channel... 29 1.9
At4g01010.1 68417.m00136 cyclic nucleotide-regulated ion channel... 29 2.6
At3g45850.1 68416.m04962 kinesin motor protein-related kinesin-r... 29 2.6
At2g46430.1 68415.m05778 cyclic nucleotide-regulated ion channel... 29 2.6
At1g77640.1 68414.m09039 AP2 domain-containing transcription fac... 29 2.6
At1g27170.1 68414.m03310 disease resistance protein (TIR-NBS-LRR... 28 5.9
At5g62090.2 68418.m07793 expressed protein 27 7.8
At5g62090.1 68418.m07792 expressed protein 27 7.8
At5g09640.1 68418.m01115 sinapoylglucose:choline sinapoyltransfe... 27 7.8
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 7.8
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 7.8
At1g21790.1 68414.m02727 expressed protein 27 7.8
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 217 bits (531), Expect = 4e-57
Identities = 107/201 (53%), Positives = 137/201 (68%), Gaps = 3/201 (1%)
Frame = +2
Query: 38 SLEAIVNNNLSGKDLEEFNRIYYGR---KDHFEIKLKDTSXXXXXXXXXXXXXXXFPAKK 208
SL +++ NL + +E NR+ GR + +I L +++ F A K
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 209 EQTRPPRIVKVGIIQHSIAIPTDRPINEQKKAIFDKVKKIIDVAGQEGVNIICFQELWNM 388
EQ R PR+V+VG+IQ+SIA+PT P ++Q + IFDK+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 389 PFAFCTREKQPWCEFAESAEEGPTTRFLRELAVKYAMVIVSSILERDEKHADFLWNTTVI 568
PFAFCTRE++ WCEFAE +G +T+FL+ELA KY MVIVS ILERD H + LWNT VI
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 569 ISDTGNVIGKHRKNHIPRVGD 631
I + GN+IGKHRKNHIPRVGD
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGD 220
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 51.6 bits (118), Expect = 4e-07
Identities = 30/115 (26%), Positives = 53/115 (46%), Gaps = 4/115 (3%)
Frame = +2
Query: 284 INEQKKAIFDKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEG--- 454
+ KK KK I+ A +G ++ E+WN P++ + +AE + G
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151
Query: 455 -PTTRFLRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNHI 616
P+T L E++ + + I+ + E+ D L+NT + G + KHRK H+
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 51.6 bits (118), Expect = 4e-07
Identities = 30/115 (26%), Positives = 53/115 (46%), Gaps = 4/115 (3%)
Frame = +2
Query: 284 INEQKKAIFDKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEG--- 454
+ KK KK I+ A +G ++ E+WN P++ + +AE + G
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151
Query: 455 -PTTRFLRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNHI 616
P+T L E++ + + I+ + E+ D L+NT + G + KHRK H+
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 48.0 bits (109), Expect = 5e-06
Identities = 31/101 (30%), Positives = 56/101 (55%), Gaps = 1/101 (0%)
Frame = +2
Query: 320 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEGPTTRFLRELAVKYAM 499
++++ A +G NII QEL+ + FC +++ + + A+ + PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 500 VI-VSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNHIP 619
VI VS E + H +N+ II G +G +RK+HIP
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIP 126
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 46.8 bits (106), Expect = 1e-05
Identities = 31/99 (31%), Positives = 54/99 (54%), Gaps = 1/99 (1%)
Frame = +2
Query: 326 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEGPTTRFLRELAVKYAMVI 505
++ A +G NII QEL+ + FC +++ + + A+ + PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 506 -VSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNHIP 619
VS E + H +N+ II G +G +RK+HIP
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIP 153
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 33.9 bits (74), Expect = 0.090
Identities = 41/142 (28%), Positives = 59/142 (41%), Gaps = 13/142 (9%)
Frame = +2
Query: 221 PPRIVKVGIIQHSIAIPTDRPINEQKKAIFDKVKKIIDVAGQEGVNIICFQELW------ 382
P V+V I+Q S + D P A DK +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 383 --NMPFAFCTREKQPWCEFAE---SAEE--GPTTRFLRELAVKYAMVIVSSILERDEKHA 541
A ++ EF SA + GP L ELA K + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 542 DFLWNTTVIISDTGNVIGKHRK 607
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 31.9 bits (69), Expect = 0.36
Identities = 29/115 (25%), Positives = 51/115 (44%), Gaps = 13/115 (11%)
Frame = +2
Query: 302 AIFDKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 448
A DK ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 449 E--GPTTRFLRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRK 607
+ GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 31.9 bits (69), Expect = 0.36
Identities = 19/52 (36%), Positives = 26/52 (50%)
Frame = +2
Query: 452 GPTTRFLRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRK 607
GP L ELA K + +V +E+D L+ T + S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At5g53130.1 68418.m06604 cyclic nucleotide-regulated ion channel /
cyclic nucleotide-gated channel (CNGC1) almost identical
to cyclic nucleotide-regulated ion channel 1 pir:T51354,
GI:11357236 from [Arabidopsis thaliana]
Length = 716
Score = 31.1 bits (67), Expect = 0.64
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = +1
Query: 25 PRNPKP*SDRQQQLKWQRPRGVQQDLLWTERP--FRNQIKRYI 147
P N + R +Q KWQ RGV ++ L + P R IKR++
Sbjct: 434 PENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHL 476
>At4g21780.1 68417.m03151 expressed protein
Length = 115
Score = 31.1 bits (67), Expect = 0.64
Identities = 22/65 (33%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = +3
Query: 336 SPVKRELI*FVSKNYGTC-PSLSARGRNNPGANSLNLPRKDRLHAFSVSWLSNTPW*SCL 512
S V++E+ F KN+ TC P R R N N ++DR + SW S T W
Sbjct: 15 SLVRKEIDYF--KNFSTCHPQFDPR-RGNSEENKNTFKQRDRSSSRLGSWFSKTKWRKKK 71
Query: 513 LSSRG 527
+RG
Sbjct: 72 RQTRG 76
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.9 bits (64), Expect = 1.5
Identities = 38/142 (26%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 221 PPRIVKVGIIQHSIAIPTDRPINEQKKAIFDKVKKIIDVAGQEGVNIICFQE--LWNMPF 394
P V+V I+Q S + D P A DK +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 395 AFC---------TREKQPWCEFAESAEE--GPTTRFLRELAVKYAMVIVSSILERDEKHA 541
F + + ++ SA GP L ++A K + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 542 DFLWNTTVIISDTGNVIGKHRK 607
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At1g01340.1 68414.m00049 cyclic nucleotide-regulated ion channel
(CNGC10) (ACBK1) almost identical to CaM-regulated
potassium ion channel (ACBK1) GI:8515883 from
[Arabidopsis thaliana]; contains Pfam domain, PF00520:
Ion transport protein
Length = 706
Score = 29.5 bits (63), Expect = 1.9
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 2/34 (5%)
Frame = +1
Query: 52 RQQQLKWQRPRGVQQDLLWTERP--FRNQIKRYI 147
R +Q +WQ RGV+++ L P R IKR++
Sbjct: 425 RYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHL 458
>At4g01010.1 68417.m00136 cyclic nucleotide-regulated ion channel,
putative (CNGC13) similar to CaM-regulated potassium ion
channel (ACBK1) GI:8515883 from [Arabidopsis thaliana]
Length = 696
Score = 29.1 bits (62), Expect = 2.6
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +1
Query: 52 RQQQLKWQRPRGVQQDLLWTERP--FRNQIKRY 144
R +Q KWQ RGV+++ L P R IKR+
Sbjct: 431 RYEQYKWQETRGVEEENLLRNLPKDLRRDIKRH 463
>At3g45850.1 68416.m04962 kinesin motor protein-related
kinesin-related protein TKRP125, Nicotiana tabacum,
PIR:T02017
Length = 1058
Score = 29.1 bits (62), Expect = 2.6
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 2/83 (2%)
Frame = +2
Query: 251 QHSIAIPTDRPINEQ--KKAIFDKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPW 424
++ I IP DR I E+ KKA+ +K++++ + + ++ QEL+N T E
Sbjct: 426 KNGIYIPKDRYIQEEAEKKAMAEKIERLELQSESKDKRVVDLQELYNSQ-QILTAELSEK 484
Query: 425 CEFAESAEEGPTTRFLRELAVKY 493
E E E T L +L KY
Sbjct: 485 LEKTEKKLE-ETEHSLFDLEEKY 506
>At2g46430.1 68415.m05778 cyclic nucleotide-regulated ion channel /
cyclic nucleotide-gated channel (CNGC3) identical to
cyclic nucleotide and calmodulin-regulated ion channel
GI:4581201 from [Arabidopsis thaliana]
Length = 706
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = +1
Query: 52 RQQQLKWQRPRGVQQDLLWTERP--FRNQIKRYI 147
+ +Q KWQ +GV+++ L + P R IKR++
Sbjct: 434 KYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHL 467
>At1g77640.1 68414.m09039 AP2 domain-containing transcription
factor, putative Similar to DREB1A (GP:3660548)
[Arabidopsis thaliana]
Length = 244
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 450 SSADSANSHQGCFSRVQKAKGMFHSSW 370
SS+ SA HQ C ++++K KG+ SW
Sbjct: 25 SSSTSALRHQSCKNKIKKYKGVRMRSW 51
>At1g27170.1 68414.m03310 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1544
Score = 27.9 bits (59), Expect = 5.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = -2
Query: 586 VSRVTNNNRRIPKKVGVFLVPLEDRRHDYHGVFDSQ 479
V RV P+KVG F+V LE D G+ D++
Sbjct: 331 VKRVLAELSNTPEKVGEFIVGLESPLKDLTGLIDTE 366
>At5g62090.2 68418.m07793 expressed protein
Length = 816
Score = 27.5 bits (58), Expect = 7.8
Identities = 28/108 (25%), Positives = 44/108 (40%)
Frame = +2
Query: 290 EQKKAIFDKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEGPTTRF 469
E KA+ + V + I V + + II QEL + + FCTR + E P
Sbjct: 410 EYGKAVQESVYEHIRVVREGHLRIIFSQELKILSWEFCTRRHE---ELLPRRLVAPQVNQ 466
Query: 470 LRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNH 613
L ++A K I S D H L + ++ G + K ++H
Sbjct: 467 LLQVAEKCQSTIDQS--GSDGIHQQDLQANSNMVMAAGRQLAKSLESH 512
>At5g62090.1 68418.m07792 expressed protein
Length = 816
Score = 27.5 bits (58), Expect = 7.8
Identities = 28/108 (25%), Positives = 44/108 (40%)
Frame = +2
Query: 290 EQKKAIFDKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEEGPTTRF 469
E KA+ + V + I V + + II QEL + + FCTR + E P
Sbjct: 410 EYGKAVQESVYEHIRVVREGHLRIIFSQELKILSWEFCTRRHE---ELLPRRLVAPQVNQ 466
Query: 470 LRELAVKYAMVIVSSILERDEKHADFLWNTTVIISDTGNVIGKHRKNH 613
L ++A K I S D H L + ++ G + K ++H
Sbjct: 467 LLQVAEKCQSTIDQS--GSDGIHQQDLQANSNMVMAAGRQLAKSLESH 512
>At5g09640.1 68418.m01115 sinapoylglucose:choline
sinapoyltransferase (SNG2) GC donor splice site at exon
11 and 13; TA donor splice site at exon 10; similar to
serine carboxypeptidase I precursor (SP:P37890) [Oryza
sativa]; wound-inducible carboxypeptidase, Lycopersicon
esculentum, EMBL:AF242849; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:choline sinapoyltransferase (SNG2)
GI:15418806
Length = 465
Score = 27.5 bits (58), Expect = 7.8
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = -2
Query: 394 EGHVP*FLETNYINSLLTGDIDYLFYLIEN 305
EG +P LET Y++ +GD++ +Y +++
Sbjct: 34 EGPLPFELETGYVSIGESGDVELFYYFVKS 63
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 7.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 569 ISDTGNVIGKHRKNHIP 619
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 7.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 569 ISDTGNVIGKHRKNHIP 619
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g21790.1 68414.m02727 expressed protein
Length = 288
Score = 27.5 bits (58), Expect = 7.8
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = -2
Query: 331 DYLFYLIENCFFLLVNWTV-GWY-RDRVLNNTN 239
+ L+ + CFF L+NW V W RD + NT+
Sbjct: 42 ELLWVICGGCFFQLMNWVVRSWISRDPIFVNTS 74
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,442,565
Number of Sequences: 28952
Number of extensions: 305485
Number of successful extensions: 840
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 810
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 834
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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