BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_C05
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52550.1 68418.m06525 expressed protein 30 0.52
At2g02120.1 68415.m00148 plant defensin-fusion protein, putative... 29 0.91
At5g10080.1 68418.m01168 aspartyl protease family protein contai... 29 1.6
At5g06580.1 68418.m00743 FAD linked oxidase family protein simil... 27 3.7
At4g04650.1 68417.m00680 hypothetical protein 26 8.5
At3g30240.1 68416.m03821 hypothetical protein 26 8.5
At2g44900.1 68415.m05589 armadillo/beta-catenin repeat family pr... 26 8.5
At2g19920.1 68415.m06036 RNA-dependent RNA polymerase family pro... 26 8.5
>At5g52550.1 68418.m06525 expressed protein
Length = 360
Score = 30.3 bits (65), Expect = 0.52
Identities = 14/52 (26%), Positives = 23/52 (44%)
Frame = -3
Query: 200 DSCSDGVRRWYCLNISSVPWDHVEARARRCSGGVSAGKRHHRCNCYYVSEHH 45
+ C D R PWD+ + SGG++ H RC+ Y V +++
Sbjct: 250 EDCDDSYRNTLNQETGFKPWDYT-TKINLFSGGINRFFPHQRCSSYAVHDNN 300
>At2g02120.1 68415.m00148 plant defensin-fusion protein, putative
(PDF2.1) plant defensin protein family member, personal
communication, Bart Thomma
(Bart.Thomma@agr.kuleuven.ac.be); contains a
gamma-thionin family signature (PDOC00725)
Length = 77
Score = 29.5 bits (63), Expect = 0.91
Identities = 12/26 (46%), Positives = 14/26 (53%), Gaps = 1/26 (3%)
Frame = -2
Query: 318 SKCRRSCHPDELPGG-CRPFCLRVFC 244
+ C CH + PGG CR F R FC
Sbjct: 48 TNCENVCHNEGFPGGDCRGFRRRCFC 73
>At5g10080.1 68418.m01168 aspartyl protease family protein contains
Eukaryotic and viral aspartyl proteases active site,
PROSITE:PS00141
Length = 528
Score = 28.7 bits (61), Expect = 1.6
Identities = 16/50 (32%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Frame = +3
Query: 108 GAPPSACF-DMIPGHAADVQTVPAPYTITTAVSSVKAGHSIDVVISGKTP 254
G PS C D I A + +P + T + GH++ I+GKTP
Sbjct: 447 GWSPSKCQEDKIEPPQASPGSTSSPNPLPTDEQQSRGGHAVSPAIAGKTP 496
>At5g06580.1 68418.m00743 FAD linked oxidase family protein similar
to SP|Q12627 from Kluyveromyces lactis and SP|P32891
from Saccharomyces cerevisiae; contains Pfam FAD linked
oxidases, C-terminal domain PF02913, Pfam FAD binding
domain PF01565
Length = 567
Score = 27.5 bits (58), Expect = 3.7
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -1
Query: 355 LRGCEKV-SSGLTVQVPTILSP*RASRRMPAILSSGVLPL 239
LR C ++ S+ + T+LSP + RR+P SS + PL
Sbjct: 17 LRPCRQLHSTPKSTGDVTVLSPVKGRRRLPTCWSSSLFPL 56
>At4g04650.1 68417.m00680 hypothetical protein
Length = 332
Score = 26.2 bits (55), Expect = 8.5
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 149 VPWDHVEARARRCSGGVSAGKRHHRCNCYYVSEHHLFYKFQ 27
V W+ + R R + G+S CN + S HLF++ Q
Sbjct: 149 VAWNRLHTRDRLQNWGLSIPAECLLCNAHDDSRAHLFFECQ 189
>At3g30240.1 68416.m03821 hypothetical protein
Length = 189
Score = 26.2 bits (55), Expect = 8.5
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = -3
Query: 404 FFGMHLVGHGVIWFTTVEG 348
FF +LVGH + FT+VEG
Sbjct: 84 FFAENLVGHALDSFTSVEG 102
>At2g44900.1 68415.m05589 armadillo/beta-catenin repeat family
protein / F-box family protein contains similarity to
F-box protein FBL2 GI:6010699 from [Rattus norvegicus];
contains Pfam profiles PF00514:
Armadillo/beta-catenin-like repeat, PF00646: F-box
domain
Length = 930
Score = 26.2 bits (55), Expect = 8.5
Identities = 11/47 (23%), Positives = 20/47 (42%)
Frame = -3
Query: 197 SCSDGVRRWYCLNISSVPWDHVEARARRCSGGVSAGKRHHRCNCYYV 57
S + + W CL SS W ++ R + ++A N +Y+
Sbjct: 69 SLASTCKTWRCLGASSCLWTSLDLRPHKFDASMAASLASRCVNLHYL 115
>At2g19920.1 68415.m06036 RNA-dependent RNA polymerase family
protein contains Pfam domain, PF05183: RNA dependent RNA
polymerase
Length = 927
Score = 26.2 bits (55), Expect = 8.5
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +2
Query: 164 DSTSALHHHYSCQLGKSWSFH*RGYQRQNT 253
+S S H+Y C + S+ +GY +NT
Sbjct: 207 ESDSGKTHYYQCHVAPDGSYRLKGYFLENT 236
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,213,881
Number of Sequences: 28952
Number of extensions: 220568
Number of successful extensions: 584
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 584
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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