Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_B24
(520 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha ch... 56 1e-08
At1g03090.1 68414.m00283 methylcrotonyl-CoA carboxylase alpha ch... 56 1e-08
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 39 0.002
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 38 0.003
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 33 0.12
At5g16390.2 68418.m01916 biotin carboxyl carrier protein 1 (BCCP... 33 0.15
At5g41900.1 68418.m05101 hydrolase, alpha/beta fold family prote... 31 0.47
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 31 0.61
At3g56130.2 68416.m06239 biotin/lipoyl attachment domain-contain... 30 1.1
At3g56130.1 68416.m06238 biotin/lipoyl attachment domain-contain... 30 1.1
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 30 1.1
At5g52790.1 68418.m06551 CBS domain-containing protein-related c... 28 3.3
At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar... 28 4.3
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 27 5.7
At5g23870.3 68418.m02803 pectinacetylesterase family protein con... 27 7.6
At1g53190.1 68414.m06028 zinc finger (C3HC4-type RING finger) fa... 27 7.6
At1g47330.1 68414.m05240 expressed protein contains Pfam profile... 27 7.6
>At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha
chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase
1 (MCCA) nearly identical to SP|Q42523
Methylcrotonyl-CoA carboxylase alpha chain,
mitochondrial precursor (EC 6.4.1.4)
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) {Arabidopsis thaliana}
Length = 734
Score = 56.4 bits (130), Expect = 1e-08
Identities = 26/65 (40%), Positives = 42/65 (64%)
Frame = +1
Query: 22 APMPGTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKLEGDDL 201
APM G V+ + V+ KV++GQPI VL AMKME +V+AP +G ++ + + GQ++
Sbjct: 669 APMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDGSA 728
Query: 202 ICTIE 216
+ I+
Sbjct: 729 LFRIK 733
>At1g03090.1 68414.m00283 methylcrotonyl-CoA carboxylase alpha
chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase
1 (MCCA) nearly identical to SP|Q42523
Methylcrotonyl-CoA carboxylase alpha chain,
mitochondrial precursor (EC 6.4.1.4)
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) {Arabidopsis thaliana}
Length = 714
Score = 56.4 bits (130), Expect = 1e-08
Identities = 26/65 (40%), Positives = 42/65 (64%)
Frame = +1
Query: 22 APMPGTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKLEGDDL 201
APM G V+ + V+ KV++GQPI VL AMKME +V+AP +G ++ + + GQ++
Sbjct: 649 APMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDGSA 708
Query: 202 ICTIE 216
+ I+
Sbjct: 709 LFRIK 713
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 38.7 bits (86), Expect = 0.002
Identities = 19/58 (32%), Positives = 35/58 (60%)
Frame = +1
Query: 22 APMPGTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKLEGD 195
+P PG +KV GDKV+KGQ + ++ AMK+ ++A ++G + + +G+ + D
Sbjct: 192 SPGPGEPPFVKV--GDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVD 247
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 38.3 bits (85), Expect = 0.003
Identities = 20/65 (30%), Positives = 36/65 (55%)
Frame = +1
Query: 22 APMPGTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKLEGDDL 201
+P PG IKV GDKV+KGQ + ++ AMK+ +++ G V + +G+ + D
Sbjct: 217 SPAPGEPPFIKV--GDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTP 274
Query: 202 ICTIE 216
+ ++
Sbjct: 275 LFVVQ 279
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 33.1 bits (72), Expect = 0.12
Identities = 15/60 (25%), Positives = 31/60 (51%)
Frame = +1
Query: 34 GTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKLEGDDLICTI 213
GT+ K GD+VE + IA + K+ + + +P +G+++ + G +E + + I
Sbjct: 108 GTLAAFLKKPGDRVEADEAIAQIETDKVTIDIASPASGVIQEFLVKEGDTVEPGNKVARI 167
>At5g16390.2 68418.m01916 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 254
Score = 32.7 bits (71), Expect = 0.15
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = +1
Query: 22 APMPGTVLTIKVKEGDKVEKGQPIAVLSAMKM 117
+P PG IKV GDKV+KGQ + ++ AMK+
Sbjct: 217 SPAPGEPPFIKV--GDKVQKGQVLCIVEAMKL 246
>At5g41900.1 68418.m05101 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas putida] GI:2822275; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 471
Score = 31.1 bits (67), Expect = 0.47
Identities = 10/38 (26%), Positives = 24/38 (63%)
Frame = +2
Query: 344 VLKPCDAYSANDDVTRARARQLIILPTLLTGSSTSPKW 457
V++ CD + + +++ + R + + T++ SST+P+W
Sbjct: 119 VVESCDHHESKKKISKGKKRLMTLNSTVIEKSSTAPRW 156
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 30.7 bits (66), Expect = 0.61
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +1
Query: 34 GTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQK 183
G V+ KEGDKVE G + + K + ++ G + + +T G K
Sbjct: 100 GNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKILVTEGSK 149
>At3g56130.2 68416.m06239 biotin/lipoyl attachment domain-containing
protein low similarity to SP|Q06881 Biotin carboxyl
carrier protein of acetyl-CoA carboxylase (BCCP)
{Anabaena sp.}; contains Pfam profile PF00364:
Biotin-requiring enzyme
Length = 205
Score = 29.9 bits (64), Expect = 1.1
Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 58 KEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKL-EGDDLICTIE*IH 225
KEGD +++GQ I L + E+ V + AG V + +G + GD L+ + H
Sbjct: 144 KEGDAIKEGQVIGYLHQLGTELPVTSDVAGEVLKLLSDDGDSVGYGDPLVAVLPSFH 200
>At3g56130.1 68416.m06238 biotin/lipoyl attachment domain-containing
protein low similarity to SP|Q06881 Biotin carboxyl
carrier protein of acetyl-CoA carboxylase (BCCP)
{Anabaena sp.}; contains Pfam profile PF00364:
Biotin-requiring enzyme
Length = 281
Score = 29.9 bits (64), Expect = 1.1
Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 58 KEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQKL-EGDDLICTIE*IH 225
KEGD +++GQ I L + E+ V + AG V + +G + GD L+ + H
Sbjct: 220 KEGDAIKEGQVIGYLHQLGTELPVTSDVAGEVLKLLSDDGDSVGYGDPLVAVLPSFH 276
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 29.9 bits (64), Expect = 1.1
Identities = 13/48 (27%), Positives = 27/48 (56%)
Frame = +1
Query: 34 GTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNG 177
G +++ EGDK+ KG+ + V+ + K +M V+ G + ++ + G
Sbjct: 54 GKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVEEG 101
>At5g52790.1 68418.m06551 CBS domain-containing protein-related
contains Pfam profile PF01595: Domain of unknown
function, weak hit to PF00571: CBS domain
Length = 500
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/27 (44%), Positives = 21/27 (77%)
Frame = +2
Query: 404 QLIILPTLLTGSSTSPKWLPVFVDTLI 484
Q ++L TLL G++ + + LP+FVD+L+
Sbjct: 70 QHLLLCTLLIGNALAMEALPIFVDSLL 96
>At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar to
RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF00397:
WW domain
Length = 1088
Score = 27.9 bits (59), Expect = 4.3
Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
Frame = +3
Query: 126 RSGPKSWARQVSRHHQRSETRGG--RPHLYHRMNSYMY 233
RS +SW+R SR + S RGG R Y R S +Y
Sbjct: 985 RSRSRSWSRSRSRSPRHSRDRGGHNRSRSYSRSPSPVY 1022
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 27.5 bits (58), Expect = 5.7
Identities = 12/49 (24%), Positives = 28/49 (57%)
Frame = +1
Query: 34 GTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPRAGLVKSVDITNGQ 180
G +++ EG+K+ KG+ + V+ + K +M V+ G + ++ + G+
Sbjct: 70 GKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEGE 118
>At5g23870.3 68418.m02803 pectinacetylesterase family protein
contains Pfam profile: PF03283 pectinacetylesterase
Length = 451
Score = 27.1 bits (57), Expect = 7.6
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = -3
Query: 380 HHSPNTHRTVLILQLYIKNIFFFYYIVFFSE 288
H+S TH T L L L N FFF+ I FS+
Sbjct: 419 HNSRGTHLTFLFLLL---NFFFFFVISKFSK 446
>At1g53190.1 68414.m06028 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 finger protein RHG1a
GI:3822225 from [Arabidopsis thaliana]; contains Pfam
profile PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 494
Score = 27.1 bits (57), Expect = 7.6
Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +3
Query: 78 ERSADSRPLGHEDGDDRSGP-KSWARQVSRHHQRSET 185
+ + +S PLG E G GP + ++ +HHQR ++
Sbjct: 297 QNTMNSGPLGSEMGSSHMGPVQPTGFRIYQHHQRDDS 333
>At1g47330.1 68414.m05240 expressed protein contains Pfam profile
PF01595: Domain of unknown function
Length = 527
Score = 27.1 bits (57), Expect = 7.6
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +2
Query: 404 QLIILPTLLTGSSTSPKWLPVFVDTLI 484
Q ++L TLL G+S + + LP+F+D ++
Sbjct: 69 QHLLLCTLLIGNSMAMEALPIFLDKIV 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,114,561
Number of Sequences: 28952
Number of extensions: 189118
Number of successful extensions: 517
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 517
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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