Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_B17
(570 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g43750.1 68416.m04674 zinc finger (C3HC4-type RING finger) fa... 30 0.95
At5g45340.2 68418.m05566 cytochrome P450 family protein similar ... 30 1.2
At5g45340.1 68418.m05565 cytochrome P450 family protein similar ... 30 1.2
At2g45670.1 68415.m05678 calcineurin B subunit-related contains ... 29 2.2
At4g19230.2 68417.m02837 cytochrome P450 family protein cytochro... 29 2.9
At4g19230.1 68417.m02836 cytochrome P450 family protein cytochro... 29 2.9
At4g01970.1 68417.m00262 galactinol-raffinose galactosyltransfer... 29 2.9
At3g46160.1 68416.m04995 protein kinase-related contains eukaryo... 29 2.9
At5g40390.1 68418.m04899 raffinose synthase family protein simil... 28 5.0
At3g53640.1 68416.m05925 protein kinase family protein contains ... 28 5.0
At2g07505.1 68415.m00868 hypothetical protein and genefinder 27 6.7
At1g79190.1 68414.m09233 expressed protein 27 6.7
At5g47020.1 68418.m05795 glycine-rich protein strong similarity ... 27 8.8
At3g10870.1 68416.m01309 hydrolase, alpha/beta fold family prote... 27 8.8
At2g22300.1 68415.m02646 ethylene-responsive calmodulin-binding ... 27 8.8
>At3g43750.1 68416.m04674 zinc finger (C3HC4-type RING finger)
family protein contains a zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 346
Score = 30.3 bits (65), Expect = 0.95
Identities = 13/35 (37%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +1
Query: 133 NNIRLKVVRNYDLWSRFV-MDHDIVEVDILNVVVC 234
N+I+LKV+ N WS+ H I ++ NV++C
Sbjct: 304 NDIKLKVLANQQKWSQCAKCQHMIARIEGCNVIIC 338
>At5g45340.2 68418.m05566 cytochrome P450 family protein similar to
SP|Q42569|C901_ARATH Cytochrome P450 90A1 (SP:Q42569)
[Arabidopsis thaliana]; contains Pfam profile: PF00067:
Cytochrome P450
Length = 446
Score = 29.9 bits (64), Expect = 1.2
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = -1
Query: 411 LLLPKGRVGGMPYVLMVYISEFHPPNVSYSASEDKYGSRFLSANPLGFP 265
L LP G +G PYV + PNV ++A + +YGS F + LG P
Sbjct: 34 LPLPPGTMG-YPYVGETFQLYSQDPNVFFAAKQRRYGSVF-KTHVLGCP 80
>At5g45340.1 68418.m05565 cytochrome P450 family protein similar to
SP|Q42569|C901_ARATH Cytochrome P450 90A1 (SP:Q42569)
[Arabidopsis thaliana]; contains Pfam profile: PF00067:
Cytochrome P450
Length = 463
Score = 29.9 bits (64), Expect = 1.2
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = -1
Query: 411 LLLPKGRVGGMPYVLMVYISEFHPPNVSYSASEDKYGSRFLSANPLGFP 265
L LP G +G PYV + PNV ++A + +YGS F + LG P
Sbjct: 34 LPLPPGTMG-YPYVGETFQLYSQDPNVFFAAKQRRYGSVF-KTHVLGCP 80
>At2g45670.1 68415.m05678 calcineurin B subunit-related contains
Pfam PF00036: EF hand domain and Prosite PS00018:
EF-hand calcium-binding domain; contains Pfam profile
PF01553: Acyltransferase; weak similarity to Calcineurin
B subunit isoform 2 (Protein phosphatase 2B regulatory
subunit 2) (Protein phosphatase 3 regulatory subunit B
alpha isoform 2) (Swiss-Prot:Q63811) [Mus musculus]
Length = 539
Score = 29.1 bits (62), Expect = 2.2
Identities = 20/77 (25%), Positives = 33/77 (42%), Gaps = 2/77 (2%)
Frame = -1
Query: 564 SVIVKESTENL-FTVDDFKSANEIYVKAQNIVEGKKETLE-ERTRHCDLFPHHLLLPKGR 391
+++ ES ++L F ++ IYV + K E +R CD FP LL P+G
Sbjct: 194 TIVASESHDSLPFVGTIIRAMQVIYVNRFSQTSRKNAVHEIKRKASCDRFPRLLLFPEGT 253
Query: 390 VGGMPYVLMVYISEFHP 340
++ + F P
Sbjct: 254 TTNGKVLISFQLGAFIP 270
>At4g19230.2 68417.m02837 cytochrome P450 family protein cytochrome
P450, Arabidopsis thaliana; supported by cDNA:
gi_15293092_gb_AY050980.1_
Length = 484
Score = 28.7 bits (61), Expect = 2.9
Identities = 18/49 (36%), Positives = 25/49 (51%)
Frame = -1
Query: 411 LLLPKGRVGGMPYVLMVYISEFHPPNVSYSASEDKYGSRFLSANPLGFP 265
L LP G +G PYV + PNV + + + +YGS F + LG P
Sbjct: 34 LPLPPGTMG-WPYVGETFQLYSQDPNVFFQSKQKRYGSVF-KTHVLGCP 80
>At4g19230.1 68417.m02836 cytochrome P450 family protein cytochrome
P450, Arabidopsis thaliana; supported by cDNA:
gi_15293092_gb_AY050980.1_
Length = 467
Score = 28.7 bits (61), Expect = 2.9
Identities = 18/49 (36%), Positives = 25/49 (51%)
Frame = -1
Query: 411 LLLPKGRVGGMPYVLMVYISEFHPPNVSYSASEDKYGSRFLSANPLGFP 265
L LP G +G PYV + PNV + + + +YGS F + LG P
Sbjct: 34 LPLPPGTMG-WPYVGETFQLYSQDPNVFFQSKQKRYGSVF-KTHVLGCP 80
>At4g01970.1 68417.m00262 galactinol-raffinose
galactosyltransferase, putative similar to
galactinol-raffinose galactosyltransferase GI:6634701
from [Vigna angularis]
Length = 807
Score = 28.7 bits (61), Expect = 2.9
Identities = 14/46 (30%), Positives = 20/46 (43%)
Frame = +3
Query: 273 QEDWQIGIENHTCLRLHCRKRSVDGIQKYRPSIHTAYHQHDLSVIK 410
Q DW + +H C H R++ G Y S H H+ +IK
Sbjct: 529 QPDWDMFQSDHVCAEYHAASRAICGGPVYL-SDHLGKASHNFDLIK 573
>At3g46160.1 68416.m04995 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 393
Score = 28.7 bits (61), Expect = 2.9
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -1
Query: 486 AQNIVEGKKETLEERTRHCDLFPHHLLLP 400
A I+ G K EE+ HCD+ P +++LP
Sbjct: 175 ANEILLGLKYIHEEKIIHCDIKPKNIILP 203
>At5g40390.1 68418.m04899 raffinose synthase family protein similar
to galactinol-raffinose galactosyltransferase [Vigna
angularis] GI:6634701, seed imbibition protein
GB:AAA32975 GI:167100 from [Hordeum vulgare]; contains
Pfam profile PF05691: Raffinose synthase or seed
imbibition protein Sip1
Length = 783
Score = 27.9 bits (59), Expect = 5.0
Identities = 13/46 (28%), Positives = 19/46 (41%)
Frame = +3
Query: 273 QEDWQIGIENHTCLRLHCRKRSVDGIQKYRPSIHTAYHQHDLSVIK 410
Q DW + H C H R++ G Y I +HD ++K
Sbjct: 514 QPDWDMFQSTHPCAEFHAASRAISGGPIY---ISDCVGKHDFDLLK 556
>At3g53640.1 68416.m05925 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 642
Score = 27.9 bits (59), Expect = 5.0
Identities = 24/90 (26%), Positives = 38/90 (42%), Gaps = 3/90 (3%)
Frame = -1
Query: 501 EIYVKAQNIVEGKKETLEERTRHCDLFPHHLLLPKGRVGGMPYVLMVYISEFHPPNVSYS 322
++ V A+ + K HCD+ P ++L+ +GR M+ + +F +
Sbjct: 430 DVRVYAEQLFISLKHLKNCGVLHCDIKPDNILMNEGR-------NMLKLCDFGSAMFAGE 482
Query: 321 ASEDKY-GSRFLSANP--LGFPVDRPLHLW 241
Y SRF A LG P D PL +W
Sbjct: 483 NQVTPYLVSRFYRAPEIILGLPYDHPLDIW 512
>At2g07505.1 68415.m00868 hypothetical protein and genefinder
Length = 143
Score = 27.5 bits (58), Expect = 6.7
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = -1
Query: 567 ESVIVKESTENLFTVDDFKSANEIYVKAQNIVEGKKETLEERTRHCD 427
+ V+V+E + L +D+ + A+ K +E + ETL TR C+
Sbjct: 52 DEVMVEEMEDILPKIDELEGASLTLQKGLQALEFEMETLAMETRTCE 98
>At1g79190.1 68414.m09233 expressed protein
Length = 1274
Score = 27.5 bits (58), Expect = 6.7
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 5/35 (14%)
Frame = -1
Query: 477 IVEGKKETLEERTRH-----CDLFPHHLLLPKGRV 388
+VE K+ L+ T H C+ FPH L+ P G++
Sbjct: 389 LVERTKKWLDSTTSHVNKLLCETFPHILIHPAGKI 423
>At5g47020.1 68418.m05795 glycine-rich protein strong similarity to
unknown protein (emb|CAB87688.1)
Length = 1417
Score = 27.1 bits (57), Expect = 8.8
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -3
Query: 247 PVADYKQQHSKYQLPRCHDP 188
PV YK KY+LP C+ P
Sbjct: 829 PVCPYKCVSEKYRLPNCYTP 848
>At3g10870.1 68416.m01309 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, SP|Q43360 PIR7B protein {Oryza sativa};
contains Pfam profile PF00561: hydrolase, alpha/beta
fold family
Length = 276
Score = 27.1 bits (57), Expect = 8.8
Identities = 19/48 (39%), Positives = 25/48 (52%)
Frame = +2
Query: 413 WCGNKSQCLVRSSRVSFLPSTIF*AFT*ISLADLKSSTVNKFSVDSLT 556
WC K +CL+ S FT ++ DLKSS ++ SVDSLT
Sbjct: 32 WCWYKIKCLMEVS-----------GFT-VTCIDLKSSGIDSSSVDSLT 67
>At2g22300.1 68415.m02646 ethylene-responsive calmodulin-binding
protein, putative (SR1) identical to partial sequence of
ethylene-induced calmodulin-binding protein GI:11545505
from [Arabidopsis thaliana]; contains Pfam profiles
PF03859: CG-1 domain, PF00612: IQ calmodulin-binding
motif, and PF00023: Ankyrin repeat
Length = 1032
Score = 27.1 bits (57), Expect = 8.8
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -1
Query: 567 ESVIVKESTENLFTVDDFKSANEIYVKAQN 478
E ++ + E+ FT+ DF S E VK+QN
Sbjct: 311 EHEVLDQILESSFTMQDFASLQESMVKSQN 340
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,741,094
Number of Sequences: 28952
Number of extensions: 258970
Number of successful extensions: 647
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 646
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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