Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0003_B07
         (488 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   207   6e-55
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   203   1e-53
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    29   0.38 
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr...    27   1.5  
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc...    27   2.0  
SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni...    25   4.6  
SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S...    25   6.1  
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    25   8.1  
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe...    25   8.1  
SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces pomb...    25   8.1  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  207 bits (506), Expect = 6e-55
 Identities = 100/144 (69%), Positives = 117/144 (81%), Gaps = 1/144 (0%)
 Frame = +3

Query: 60  KIGINGFGRIGRLVLRASIDKGA-DVVAINDPFIGLDYMVYLFQYDSTHGRFKGTVEAVD 236
           K+GINGFGRIGR+VLR +I  G   VVA+NDPFI LDYM Y+F+YDSTHGRF+G+VE   
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 237 GHLVVNGKKIAVFSERDPHAIPWGQAGAEYVVESTGVFTTTDKASAHLVGGAKKVIISAP 416
           G LV++G  I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL GGAK+VIISAP
Sbjct: 65  GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124

Query: 417 SADAPMFVVGVNLEAYDPSYKVIS 488
           S DAPMFVVGVNLE ++PS KVIS
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVIS 148


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  203 bits (496), Expect = 1e-53
 Identities = 98/144 (68%), Positives = 116/144 (80%), Gaps = 1/144 (0%)
 Frame = +3

Query: 60  KIGINGFGRIGRLVLR-ASIDKGADVVAINDPFIGLDYMVYLFQYDSTHGRFKGTVEAVD 236
           K+GINGFGRIGR+VLR A + K   VVAINDPFI L+YM Y+F+YDSTHGRF G+VE  D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 237 GHLVVNGKKIAVFSERDPHAIPWGQAGAEYVVESTGVFTTTDKASAHLVGGAKKVIISAP 416
           G LV++G  I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL GGAK+VIISAP
Sbjct: 65  GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124

Query: 417 SADAPMFVVGVNLEAYDPSYKVIS 488
           S DAPM+VVGVN E ++PS KVIS
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVIS 148


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 29.1 bits (62), Expect = 0.38
 Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
 Frame = +3

Query: 33  KYTLTIIMSKIGINGFGRIGRLVLRASIDKGADVVAINDPF----IGLDYMVYLFQYDST 200
           KY L   +S +   G GR+  L++  SI K  D +    PF      + Y++   Q D  
Sbjct: 627 KYKLDSCVSTLTRMGLGRVSTLLVTDSISKCIDEITKASPFADHPAAMQYIMNAAQ-DIL 685

Query: 201 HGRFKGTVEAVD 236
             RF  T E V+
Sbjct: 686 RRRFHATSEQVE 697


>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 512

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = +1

Query: 67  ELTVLVASVAWCSVLRLIRV 126
           +L+V VA +AWC   RL+R+
Sbjct: 122 KLSVAVAELAWCPKSRLVRI 141


>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 822

 Score = 26.6 bits (56), Expect = 2.0
 Identities = 12/28 (42%), Positives = 19/28 (67%)
 Frame = -1

Query: 260 LSVYDEVSIYGLNGTLETTVSRIILEKV 177
           L +   V++ G NG  +TT+ ++ILEKV
Sbjct: 617 LDLKSRVALVGPNGAGKTTLIKLILEKV 644


>SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit
           Orc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 707

 Score = 25.4 bits (53), Expect = 4.6
 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
 Frame = -3

Query: 291 GDPSQRTQL--SSFRLRRGVHLRPQRY 217
           G P++R  L   +F +RRGVHL+  +Y
Sbjct: 163 GIPTKRKDLFPCNFFIRRGVHLKVNKY 189


>SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 609

 Score = 25.0 bits (52), Expect = 6.1
 Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 10/72 (13%)
 Frame = +3

Query: 33  KYTLTIIMS----KIGINGFGRIGRLVLRASIDKGADVVAINDPFIGLD------YMVYL 182
           +Y +TI +     ++ +N F  +G+L++  + D+  D+   ND    ++      YM Y+
Sbjct: 508 RYIITIAVGDHTGQLWLNVFDDVGKLIMHKTADELNDLQE-NDENAFMNCMAEACYMPYI 566

Query: 183 FQYDSTHGRFKG 218
           FQ  +    FKG
Sbjct: 567 FQCRAKQDNFKG 578


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 24.6 bits (51), Expect = 8.1
 Identities = 19/72 (26%), Positives = 32/72 (44%)
 Frame = -1

Query: 335 FNNVFSTSLTPWNSMGIPLREHSYLLSVYDEVSIYGLNGTLETTVSRIILEKVDHVVKTN 156
           +N +       +NS+    R  S L   + E  I+ ++ TLE+  S +     DHV K  
Sbjct: 684 YNTIMEVWNIKYNSLNALARLLSALYKFHPEFCIHVIDDTLESLFSAV--NNSDHVEK-Q 740

Query: 155 ERIIDSNNIGTL 120
           +R+  +  I  L
Sbjct: 741 KRLAQARFISEL 752


>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 551

 Score = 24.6 bits (51), Expect = 8.1
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = -1

Query: 362 ISGCEHTG*FNNVFSTSLTPWNSMGIPL 279
           +S   H+G F     T L+PWN   IPL
Sbjct: 445 VSSSRHSGIFAT--PTFLSPWNIKNIPL 470


>SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 392

 Score = 24.6 bits (51), Expect = 8.1
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -1

Query: 320 STSLTPWNSMGIPLREHSYLLSVYDEVSI 234
           S S    +S  +PLREH  L +VY +V +
Sbjct: 50  SLSSVDVSSPSLPLREHLPLKNVYSQVPL 78


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,112,773
Number of Sequences: 5004
Number of extensions: 43589
Number of successful extensions: 156
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)