Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_B05
(481 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 152 2e-38
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 122 2e-29
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 113 2e-26
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 108 5e-25
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 97 9e-22
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 83 3e-17
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 63 2e-11
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 31 0.12
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 29 0.48
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 28 0.64
SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa... 27 1.9
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 26 2.6
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 2.6
SPCC4B3.01 ||SPCP25A2.01c|thiosulfate sulfurtransferase|Schizosa... 26 3.4
SPAC959.10 |sen15||tRNA-splicing endonuclease subunit Sen15|Schi... 26 3.4
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 25 5.9
SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr... 25 5.9
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 25 7.8
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 25 7.8
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 152 bits (369), Expect = 2e-38
Identities = 65/153 (42%), Positives = 105/153 (68%)
Frame = +3
Query: 12 RLFVEETIHDEFVKRVLEETKKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEGAK 191
R++V+E ++DEF+KR++ + K + +GDP T G Q K ++++ Y E G+ GAK
Sbjct: 309 RVYVQEDVYDEFIKRMVAKAKTLKVGDPFAEDTFQGAQVSKQQYERIVSYIESGIAHGAK 368
Query: 192 LLYGGKRVDRKGFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRANNTE 371
L GGKR G+F +PT+ ++VT+DM + KEE FGP++ + KF + +E+ IRR NN+
Sbjct: 369 LEIGGKRHGNLGYFVEPTILSNVTEDMAVGKEEIFGPVLAVIKF--KTIEEAIRRGNNST 426
Query: 372 YGLASGVFTRDVSRALQFAERIDAGTVFVNTYN 470
YGLA+GV T +++ A++ + ++AGTV+VN YN
Sbjct: 427 YGLAAGVHTNNITNAIKVSNALEAGTVWVNCYN 459
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 122 bits (295), Expect = 2e-29
Identities = 59/158 (37%), Positives = 101/158 (63%), Gaps = 2/158 (1%)
Frame = +3
Query: 12 RLFVEETIHDEFVKRVLEET-KKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEGA 188
R++V+++++D+F++ + + +G P T GP +K +++ Y E G KEGA
Sbjct: 302 RIYVQDSVYDKFIELFKKHVIQDYIVGMPFDDNTVVGPVVNKTQYNRIKNYIEQGKKEGA 361
Query: 189 KLLYGGKRVDRK-GFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRANN 365
KL+ G + + K G+F PT+F D +++M I KEE FGP++ ISKF + ++ I +ANN
Sbjct: 362 KLVLGDEPLPLKQGYFISPTIFADCSENMTIVKEEIFGPVVAISKFKTE--DEAIEKANN 419
Query: 366 TEYGLASGVFTRDVSRALQFAERIDAGTVFVNTYNKTD 479
T YGLA+ FT+D+ RA + ++ ++AG VF+N+ +D
Sbjct: 420 TTYGLAAMCFTKDLERAHRVSDELEAGMVFINSTENSD 457
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 113 bits (271), Expect = 2e-26
Identities = 56/151 (37%), Positives = 92/151 (60%), Gaps = 1/151 (0%)
Frame = +3
Query: 12 RLFVEETIHDEFVKRVLEETKKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEGAK 191
R++V + ++DEFV ++ E+ K + +GD +A GP + K+ ++ E V +GAK
Sbjct: 303 RVYVHKNVYDEFVSKLTEKVKTIKVGDGFDSSSAVGPLISQDGCKKVSKHIEDAVSKGAK 362
Query: 192 LLYGGKRV-DRKGFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRANNT 368
+ GGK + KG+F +PTV + VT DM +A EE+FGP+ + KF + E+VI AN++
Sbjct: 363 ITVGGKEISSSKGYFFEPTVLSGVTQDMLVASEETFGPLASVFKFD--DTEEVIEWANDS 420
Query: 369 EYGLASGVFTRDVSRALQFAERIDAGTVFVN 461
+ GLA VFT ++S + A+ ++ G V N
Sbjct: 421 DVGLAGYVFTNNLSTMIHVAKELEVGLVGAN 451
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 108 bits (259), Expect = 5e-25
Identities = 56/156 (35%), Positives = 88/156 (56%), Gaps = 1/156 (0%)
Frame = +3
Query: 6 AGRLFVEETIHDEFVKRVLEETKKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEG 185
A RL V E++ DE + ++ E K+ IG+ L GP K +K++ Y + + EG
Sbjct: 308 ASRLIVHESVADEVLGGLVSEANKLIIGNGLDPQVTLGPVVSKTQFEKIVSYIQSAINEG 367
Query: 186 AKLLYGG-KRVDRKGFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRAN 362
K + GG R ++KG+F PTVFT+V I +EE FGP++ + F + E+ + AN
Sbjct: 368 CKCVVGGLPRSEQKGYFIPPTVFTNVQTHNKIWREEIFGPVLAVKTFHTN--EEALELAN 425
Query: 363 NTEYGLASGVFTRDVSRALQFAERIDAGTVFVNTYN 470
++EYGL SGVF+ + F+ I+AG +N Y+
Sbjct: 426 DSEYGLGSGVFSTNPKTLEFFSNNIEAGMCSLNNYH 461
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 97.5 bits (232), Expect = 9e-22
Identities = 50/154 (32%), Positives = 85/154 (55%), Gaps = 1/154 (0%)
Frame = +3
Query: 6 AGRLFVEETIHDEFVKRVLEETKKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEG 185
A R++V +++D FV V E K +G L G HGP + + K+ ++ E V++G
Sbjct: 306 ANRIYVHSSVYDAFVDLVTERVSKFKLGYGLDAGVTHGPLISEKAISKVKQHVEDAVQKG 365
Query: 186 AKLLYGGKRVDRKG-FFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRAN 362
++ GGK G + +PTV + M I++EE+FGP+ + KF + ++V+ AN
Sbjct: 366 GVVVTGGKVASNLGPMYFEPTVIINAKQGMLISEEETFGPVGALFKFDTE--DEVVAWAN 423
Query: 363 NTEYGLASGVFTRDVSRALQFAERIDAGTVFVNT 464
++ GLA +F++D+SR + E + G V NT
Sbjct: 424 DSPVGLAGYLFSKDISRVFRVGEALQVGMVGCNT 457
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 82.6 bits (195), Expect = 3e-17
Identities = 46/156 (29%), Positives = 75/156 (48%), Gaps = 4/156 (2%)
Frame = +3
Query: 12 RLFVEETIHDEFVKRVLEETKKMSIGDPLHRGTAHGPQNHKAHMDKLIEYCEVGVKEGAK 191
R+ + ++D + ++ M +G G D L + V +GA+
Sbjct: 278 RIIALDGVYDTIITKLYNRISTMRLGMYTQNDVDMGAMVSNNRFDHLESLIQDAVSKGAR 337
Query: 192 LLYGGKRVDR----KGFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRA 359
L+YGG R KG + PT+ D T++M IA+EE F PI ++ F +++ E + A
Sbjct: 338 LVYGGHRFQHPKYPKGNYFLPTLLVDATNEMKIAQEECFAPIALV--FRAKSPEHALEIA 395
Query: 360 NNTEYGLASGVFTRDVSRALQFAERIDAGTVFVNTY 467
N TE+GL + VF RD F + ++ G V VN +
Sbjct: 396 NGTEFGLGASVFGRDKQLCQYFTDNLETGMVAVNDF 431
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 63.3 bits (147), Expect = 2e-11
Identities = 46/156 (29%), Positives = 73/156 (46%), Gaps = 6/156 (3%)
Frame = +3
Query: 12 RLFVEETIHDE-FVKRVLEETKKMSIGDPLHR-GTAHGPQNHKAHMDKLIEYCEVGVKEG 185
RL+V + + F + ++ K + +G PL GP H+A +KL + E +
Sbjct: 337 RLYVSKYAWENGFRDELTKQVKSLKVGAPLTDFANFVGPVIHQASFNKLKKVLESAASDS 396
Query: 186 A-KLLYGGKRVDRKGFFSQPTVFTDVTDDMYIAKEESFGPIMIISKFSSRNMEDVIRRAN 362
++L GGK D +GFF +PTV I E FGP++ + + N++ V +
Sbjct: 397 EIEVLAGGKADDSEGFFVEPTVLLSKNPKHDIFVNELFGPVLSVYVYEDDNLDAVCDLID 456
Query: 363 -NTEYGLASGVFTRD--VSRALQFAERIDAGTVFVN 461
T YGL +F +D V R L R AG ++N
Sbjct: 457 TTTPYGLTGSIFAQDRVVVRKLTDRLRNAAGNFYIN 492
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 30.7 bits (66), Expect = 0.12
Identities = 19/60 (31%), Positives = 31/60 (51%)
Frame = +3
Query: 300 PIMIISKFSSRNMEDVIRRANNTEYGLASGVFTRDVSRALQFAERIDAGTVFVNTYNKTD 479
P+ I + SS +++ I NT+ GL+S D S+ F D T+F +Y+KT+
Sbjct: 651 PVCIYTFLSSGMLDERIFIRQNTKQGLSSSFIDSDASQKKNFFTGEDIKTLF--SYSKTE 708
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 28.7 bits (61), Expect = 0.48
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = -1
Query: 460 LTNTVPASMRSANCSARDTSRVNTPLARPYSVLFAR 353
+T +P+S S+N S +DT+ VN+P A +S F +
Sbjct: 1 MTFRIPSSQESSNKSEKDTT-VNSPNASSFSSFFKK 35
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 28.3 bits (60), Expect = 0.64
Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = -1
Query: 469 LYVLTNTVPASMRSA-NCSARDTSRVNTPLARPYSVLFARRITSSMF 332
LYV + S+ S+ C+A S+ N RPYSV+ +R S F
Sbjct: 936 LYVYKSDATNSLASSIRCTADKISKSNFDFRRPYSVIPSRMTGRSSF 982
>SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 639
Score = 26.6 bits (56), Expect = 1.9
Identities = 15/35 (42%), Positives = 16/35 (45%)
Frame = -3
Query: 308 HDRSERFLLSDVHVVGDVSEHSWLGEEPLAVDAFP 204
HDRSER GD S H +EPL V P
Sbjct: 43 HDRSER---PSSREAGDRSRHERFSKEPLEVKKLP 74
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 26.2 bits (55), Expect = 2.6
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = +3
Query: 342 DVIRRANNTEYGLASGV 392
D+I+R +TEYG+A+G+
Sbjct: 239 DIIQRKGSTEYGVAAGL 255
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 2.6
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 256 SVNTVGWEKNPLRSTRFPPYSNLAPSFTPTS 164
S ++ G+ +P P YS +PS++PTS
Sbjct: 1559 SPSSPGYSTSPAYMPSSPSYSPTSPSYSPTS 1589
Score = 25.4 bits (53), Expect = 4.5
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -1
Query: 298 PNDSSLAMYMSSVTSVNTVGWEKNPLRSTRFPPYSNLAPSFTPTS 164
P S+ YM S S + +P S P YS +PS++PTS
Sbjct: 1563 PGYSTSPAYMPSSPSYSPT----SPSYSPTSPSYSPTSPSYSPTS 1603
Score = 24.6 bits (51), Expect = 7.8
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 229 NPLRSTRFPPYSNLAPSFTPTS 164
+P S P YS +PS++PTS
Sbjct: 1603 SPSYSATSPSYSPTSPSYSPTS 1624
>SPCC4B3.01 ||SPCP25A2.01c|thiosulfate
sulfurtransferase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 298
Score = 25.8 bits (54), Expect = 3.4
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +3
Query: 216 DRKGFFSQPTVF 251
DRKGFFS P VF
Sbjct: 100 DRKGFFSSPRVF 111
>SPAC959.10 |sen15||tRNA-splicing endonuclease subunit
Sen15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 143
Score = 25.8 bits (54), Expect = 3.4
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 15 LFVEETIHDEFVKRVLEETKKMSI 86
L+++ETI EF+ V + KK+S+
Sbjct: 75 LYLQETISIEFLSSVFDSMKKLSL 98
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 25.0 bits (52), Expect = 5.9
Identities = 26/101 (25%), Positives = 38/101 (37%)
Frame = -1
Query: 469 LYVLTNTVPASMRSANCSARDTSRVNTPLARPYSVLFARRITSSMFXXXXXXXXXIGPND 290
++ LTNT+ AS + S TP + PY A T S+ D
Sbjct: 98 VFELTNTLAASPAPPSLSEPSYVMARTPSSSPYPHDRAEVGTKSLSAQFGGMSLGA---D 154
Query: 289 SSLAMYMSSVTSVNTVGWEKNPLRSTRFPPYSNLAPSFTPT 167
+ AM + + SV+ T PP NL S++ T
Sbjct: 155 GAAAMPTNELVSVDLYNQTPEISDLTSPPPPINLPLSYSAT 195
>SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 511
Score = 25.0 bits (52), Expect = 5.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -3
Query: 332 PAAKFADNHDRSERFLLSDVHVVG 261
PA + +N + ERFL+ D+ V+G
Sbjct: 475 PATNY-ENFEYEERFLIQDLEVIG 497
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 24.6 bits (51), Expect = 7.8
Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 1/25 (4%)
Frame = +3
Query: 33 IH-DEFVKRVLEETKKMSIGDPLHR 104
IH D+ +V EET +++GDP+ R
Sbjct: 64 IHQDKCTIQVYEETSGLTVGDPVQR 88
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 24.6 bits (51), Expect = 7.8
Identities = 26/108 (24%), Positives = 43/108 (39%), Gaps = 4/108 (3%)
Frame = -1
Query: 463 VLTNTVPASMRSANCSARDTS----RVNTPLARPYSVLFARRITSSMFXXXXXXXXXIGP 296
V T+T S N S TS +TP+ + + ITSS
Sbjct: 486 VNTSTPITSSTVVNSSTPITSSTALNTSTPITSSSVLNSSTPITSSTALNTSTSITSSSV 545
Query: 295 NDSSLAMYMSSVTSVNTVGWEKNPLRSTRFPPYSNLAPSFTPTSQYSI 152
+SS + S+V + +T + L S+ S + + TP ++YS+
Sbjct: 546 LNSSTPITSSTVVNTSTPITSSSVLNSSTPITSSTVVNTSTPITRYSV 593
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,683,071
Number of Sequences: 5004
Number of extensions: 31701
Number of successful extensions: 156
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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