BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0003_A24 (498 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g26840.1 68416.m03357 esterase/lipase/thioesterase family pro... 39 0.002 At3g51520.1 68416.m05642 diacylglycerol acyltransferase family c... 38 0.004 At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family prote... 33 0.14 At4g21370.1 68417.m03088 S-locus protein kinase, putative simila... 31 0.57 At1g33410.1 68414.m04136 expressed protein 27 5.3 At3g26820.1 68416.m03355 esterase/lipase/thioesterase family pro... 27 7.0 At3g08810.1 68416.m01023 kelch repeat-containing F-box family pr... 27 7.0 At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xy... 27 9.3 At1g02650.1 68414.m00215 DNAJ heat shock N-terminal domain-conta... 27 9.3 >At3g26840.1 68416.m03357 esterase/lipase/thioesterase family protein contains Pfam profile PF03096: Ndr family Length = 701 Score = 38.7 bits (86), Expect = 0.002 Identities = 35/134 (26%), Positives = 63/134 (47%), Gaps = 10/134 (7%) Frame = +3 Query: 51 GYEIRGLENLP-DGPYLVIYYHGALPVDMYYFVARMQLFKQTQIHTVADRFMFRIPGWSM 227 G +R LE LP +GP L + YH L ++ V ++ + + +A +F+ S+ Sbjct: 430 GTVVRSLEGLPSEGPVLYVGYHMILGFELAPMVIQLMTERNIHLRGLAHPMLFKNLQDSL 489 Query: 228 ----LLENLCVIPGTVQTCASV---LKNGNSLAISPGGVYEA--QFGDHYYRLNWKTRIG 380 + + ++ G + ++ L+ + + PGGV EA + G+ Y +L W R Sbjct: 490 VDTKMFDKYKIMGGVPVSHFNIYKLLREKAHVLLYPGGVREALHRKGEEY-KLFWPERSE 548 Query: 381 FAKVALEAKVPIVP 422 F +VA + IVP Sbjct: 549 FVRVASKFGAKIVP 562 >At3g51520.1 68416.m05642 diacylglycerol acyltransferase family contains Pfam PF03982: Diacylglycerol acyltransferase Length = 314 Score = 37.9 bits (84), Expect = 0.004 Identities = 30/118 (25%), Positives = 49/118 (41%), Gaps = 2/118 (1%) Frame = +3 Query: 81 PDGPYLVIYY-HGALPVDMYYFVARMQLFKQTQIHTVADRFMFRIPGWSMLLENLCVIPG 257 P+ Y+ Y H LP+ + I +A +F P + L + Sbjct: 99 PNRAYVFGYEPHSVLPIGVVALCDLTGFMPIPNIKVLASSAIFYTPFLRHIWTWLGLTAA 158 Query: 258 TVQTCASVLKNGNSLAISPGGVYEAQFGDH-YYRLNWKTRIGFAKVALEAKVPIVPMF 428 + + S+L +G S + PGGV E H + R GF ++A+E P+VP+F Sbjct: 159 SRKNFTSLLDSGYSCVLVPGGVQETFHMQHDAENVFLSRRRGFVRIAMEQGSPLVPVF 216 >At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family protein contains Pfam profile PF00561: hydrolase, alpha/beta fold family; contains non-consensus splice site (GC) at intron 10 Length = 565 Score = 32.7 bits (71), Expect = 0.14 Identities = 32/135 (23%), Positives = 59/135 (43%), Gaps = 11/135 (8%) Frame = +3 Query: 51 GYEIRGLENLP-DGPYLVIYYHGALPVDMYYFVARMQLFKQTQIHTVADRFMFR------ 209 G +RGL +P +GP L++ H L D + + + + MF Sbjct: 409 GKVVRGLGGIPSEGPVLLVGNHMLLASDKISLPGQFVHERNINLRPLVHPMMFSRLRDGL 468 Query: 210 IPGWSM--LLENLCVIPGTVQTCASVLKNGNSLAISPGGVYEA--QFGDHYYRLNWKTRI 377 +P S+ +L + +P + ++L + + + PGG+ EA + G+ Y +L W + Sbjct: 469 LPDVSVYDMLRMMGSVPISGTHLHNLLSAKSHILLFPGGIREALHRKGEEY-KLMWPEKA 527 Query: 378 GFAKVALEAKVPIVP 422 F + A + IVP Sbjct: 528 EFVRAAAKFGAKIVP 542 >At4g21370.1 68417.m03088 S-locus protein kinase, putative similar to SRKa [Arabidopsis lyrata] gi|13620927|dbj|BAB40986; contains Pfam domains PF00954: S-locus glycoprotein family, PF00069: Protein kinase domain and PF01453: Lectin (probable mannose binding) Length = 844 Score = 30.7 bits (66), Expect = 0.57 Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 4/55 (7%) Frame = +3 Query: 225 MLLENLCVIPGTVQTCASVLKNGNSLAISPGGVYEAQF----GDHYYRLNWKTRI 377 +L +L + T+ S+ + N +SPGGV+E F GD +Y W +I Sbjct: 23 ILFPDLSISVNTLSATESLTISSNKTIVSPGGVFELGFFRILGDSWYLGIWYKKI 77 >At1g33410.1 68414.m04136 expressed protein Length = 1459 Score = 27.5 bits (58), Expect = 5.3 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = +2 Query: 197 IHVSYTWMVDAPRESMRYPGNRA 265 +H Y W+ P E+ RYP +A Sbjct: 1135 VHPGYAWIDPVPEETTRYPVKKA 1157 >At3g26820.1 68416.m03355 esterase/lipase/thioesterase family protein contains Interpro entry IPR000379 Length = 634 Score = 27.1 bits (57), Expect = 7.0 Identities = 20/73 (27%), Positives = 33/73 (45%), Gaps = 2/73 (2%) Frame = +3 Query: 249 IPGTVQTCASVLKNGNSLAISPGGVYEA--QFGDHYYRLNWKTRIGFAKVALEAKVPIVP 422 +P + +L+ + + PGGV EA + G+ Y +L W + F +VA + IVP Sbjct: 436 VPVSNMNFYKLLREKAHVLLYPGGVREALHRKGEEY-KLFWPEQSEFVRVASKFGAKIVP 494 Query: 423 MFTQNVREAFRTV 461 + F V Sbjct: 495 FGVVGEDDIFNIV 507 >At3g08810.1 68416.m01023 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 343 Score = 27.1 bits (57), Expect = 7.0 Identities = 19/55 (34%), Positives = 27/55 (49%) Frame = +1 Query: 97 SLSTITELCLSICIISSHECNYSSRLKSIL*LTDSCFVYLDGRCSSRIYALSREP 261 SLS TE+CL +C+ C+ R S+ D Y + + S YAL+R P Sbjct: 67 SLSGNTEICLYVCL----RCSTGYRWFSLSRKPDQTLTYEERKSSG--YALARVP 115 >At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xylan endohydrolase isoenzyme X-I, Hordeum vulgare,PID:g1813595 Length = 576 Score = 26.6 bits (56), Expect = 9.3 Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 1/66 (1%) Frame = +3 Query: 48 HGYEIRGLENLPDGP-YLVIYYHGALPVDMYYFVARMQLFKQTQIHTVADRFMFRIPGWS 224 HG +RG L D P Y + + D+Y V R ++++V R+ ++ GW Sbjct: 285 HGIAVRGHNVLWDDPKYQPGWVNSLSGNDLYNAVKR-------RVYSVVSRYKGQLLGWD 337 Query: 225 MLLENL 242 ++ ENL Sbjct: 338 VVNENL 343 >At1g02650.1 68414.m00215 DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226: DnaJ domain Length = 513 Score = 26.6 bits (56), Expect = 9.3 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -3 Query: 436 FCVNIGTIGTLASRATLAKPILV 368 FC ++ +G+L SRA L +PI V Sbjct: 491 FCRDMAAVGSLISRAGLRQPITV 513 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,149,478 Number of Sequences: 28952 Number of extensions: 219489 Number of successful extensions: 594 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 579 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 593 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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