Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_A16
(487 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g18050.1 68416.m02296 expressed protein 29 2.2
At5g55110.1 68418.m06870 stigma-specific Stig1 family protein si... 28 3.8
At3g07550.2 68416.m00902 F-box family protein (FBL12) contains s... 28 3.8
At3g07550.1 68416.m00901 F-box family protein (FBL12) contains s... 28 3.8
At5g18430.1 68418.m02171 GDSL-motif lipase/hydrolase family prot... 27 5.1
At3g58860.1 68416.m06560 F-box family protein contains F-box dom... 27 6.7
At5g38396.1 68418.m04641 F-box family protein contains F-box dom... 27 8.9
At3g59000.1 68416.m06576 F-box family protein contains F-box dom... 27 8.9
At3g11370.1 68416.m01382 DC1 domain-containing protein contains ... 27 8.9
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 27 8.9
At2g40980.1 68415.m05062 expressed protein 27 8.9
At2g31620.1 68415.m03863 receptor-like protein kinase-related co... 27 8.9
At1g59312.1 68414.m06676 hypothetical protein 27 8.9
At1g58936.1 68414.m06664 hypothetical protein 27 8.9
At1g58643.1 68414.m06656 hypothetical protein 27 8.9
>At3g18050.1 68416.m02296 expressed protein
Length = 335
Score = 28.7 bits (61), Expect = 2.2
Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = +2
Query: 167 ETDCPAGTHITAKGLCPSQQHRGVECCHSVLRKINTCRSHGGECMDRCPENLTYK-NADD 343
ETDC +G+H A G P G C + L K+N ++ G + N T K + D
Sbjct: 193 ETDCLSGSHNNADGFSPL---LGCNKCLNSLYKLNPKKTSGTRNPSKEDRNRTAKMHNKD 249
Query: 344 C 346
C
Sbjct: 250 C 250
>At5g55110.1 68418.m06870 stigma-specific Stig1 family protein
similar to stigma-specific protein STIG1 [Nicotiana
tabacum] GI:496647; contains Pfam profile PF04885:
Stigma-specific protein, Stig1
Length = 163
Score = 27.9 bits (59), Expect = 3.8
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +2
Query: 206 GLCPSQQHRGVECCHSVLRKINTCRSHGGECMDRC 310
GLC ++ G CC + + H GEC RC
Sbjct: 115 GLCKNKCKFGQTCCRGQCVYVAYDKRHCGECNHRC 149
>At3g07550.2 68416.m00902 F-box family protein (FBL12) contains
similarity to F-box protein FBL6 GI:6456737 from [Homo
sapiens]
Length = 395
Score = 27.9 bits (59), Expect = 3.8
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 263 KINTCRSHGGECMDRCPENLTYKNADDCNIKNK 361
KI+ C+S G C L Y +AD C ++ K
Sbjct: 200 KISNCKSITGVGFSGCSPTLGYVDADSCQLEPK 232
>At3g07550.1 68416.m00901 F-box family protein (FBL12) contains
similarity to F-box protein FBL6 GI:6456737 from [Homo
sapiens]
Length = 395
Score = 27.9 bits (59), Expect = 3.8
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 263 KINTCRSHGGECMDRCPENLTYKNADDCNIKNK 361
KI+ C+S G C L Y +AD C ++ K
Sbjct: 200 KISNCKSITGVGFSGCSPTLGYVDADSCQLEPK 232
>At5g18430.1 68418.m02171 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL1 GI:15054382 from
[Arabidopsis thaliana]; contains Pfam profile PF00657:
GDSL-like Lipase/Acylhydrolase
Length = 362
Score = 27.5 bits (58), Expect = 5.1
Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Frame = +3
Query: 177 VRLVLTSLRKVFVQVNSIGA*NVVIQF*GKL--TPADLTAGNAWTG 308
VRL+++ +K+ +++NS+G V++ G L PA+L G
Sbjct: 192 VRLLISEYKKILLRLNSLGVGRVLVTGAGPLGCAPAELARSGTSNG 237
>At3g58860.1 68416.m06560 F-box family protein contains F-box domain
Pfam:PF00646
Length = 457
Score = 27.1 bits (57), Expect = 6.7
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 335 HFYTSGSQDTCPCIPRREIGRC*FSLKLN 249
H T+ D C CIPR++ GR + +N
Sbjct: 348 HTVTNKCGDVCDCIPRKKKGRSLMACPVN 376
>At5g38396.1 68418.m04641 F-box family protein contains F-box domain
Pfam:PF00646
Length = 462
Score = 26.6 bits (56), Expect = 8.9
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = -3
Query: 335 HFYTSGSQDTCPCIPRREIGR 273
HF T D C C+ R E GR
Sbjct: 362 HFVTDKCGDACDCVSREEKGR 382
>At3g59000.1 68416.m06576 F-box family protein contains F-box domain
Pfam:PF00646
Length = 491
Score = 26.6 bits (56), Expect = 8.9
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = -3
Query: 335 HFYTSGSQDTCPCIPRREIGRC*FSLKLN 249
H T D C C+PR + GR S +N
Sbjct: 364 HHVTDKCGDACDCVPREDKGRSLTSCPVN 392
>At3g11370.1 68416.m01382 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 589
Score = 26.6 bits (56), Expect = 8.9
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +2
Query: 254 VLRKINTCRSHGGECMDRCPENLTYKNADD 343
VLR N+ R C DRCP + +K D
Sbjct: 544 VLRNSNSTRPQCTRCGDRCPGYIYFKRKRD 573
>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
identical to auxin transport protein; BIG [Arabidopsis
thaliana] GI:21779966; contains Pfam profiles PF02207:
Putative zinc finger in N-recognin, PF00569: Zinc finger
ZZ type
Length = 5098
Score = 26.6 bits (56), Expect = 8.9
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = -1
Query: 208 TFRSDVSTSRTVRFSFGTLPA 146
TF++DVST++T+ +G L A
Sbjct: 855 TFQNDVSTAKTLNLDYGDLSA 875
>At2g40980.1 68415.m05062 expressed protein
Length = 617
Score = 26.6 bits (56), Expect = 8.9
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +2
Query: 236 VECCHSVLRKINTCRSHGGECMDRCPENLTY 328
+ CCH L +++ S G D PEN+ Y
Sbjct: 449 IRCCHDCLSALSSSSSAGIRHGDIRPENVVY 479
>At2g31620.1 68415.m03863 receptor-like protein kinase-related
contains Pfam profile: PF01657 Domain of unknown
function that is usually associated with protein kinase
domain Pfam:PF00069; similar to receptor-like protein
kinase 4 (GI:13506745) and receptor-like protein kinase
5 (GI:13506747) [Arabidopsis thaliana]
Length = 255
Score = 26.6 bits (56), Expect = 8.9
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 485 GQRRLKPSCRIRYQAYRYRRP 423
G R L PSC RY+ Y + +P
Sbjct: 235 GGRVLNPSCTFRYELYPFVKP 255
>At1g59312.1 68414.m06676 hypothetical protein
Length = 486
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = +2
Query: 326 YKNADDCNIKNKICCILVXXXXXXXXXXXXXLEVDGIDKLDIE 454
+K DDC ++ LV LEV +DK +IE
Sbjct: 311 FKTEDDCGLRANAFIELVAETVYASGALDQLLEVQKLDKYNIE 353
>At1g58936.1 68414.m06664 hypothetical protein
Length = 486
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = +2
Query: 326 YKNADDCNIKNKICCILVXXXXXXXXXXXXXLEVDGIDKLDIE 454
+K DDC ++ LV LEV +DK +IE
Sbjct: 311 FKTEDDCGLRANAFIELVAETVYASGALDQLLEVQKLDKYNIE 353
>At1g58643.1 68414.m06656 hypothetical protein
Length = 486
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/43 (30%), Positives = 18/43 (41%)
Frame = +2
Query: 326 YKNADDCNIKNKICCILVXXXXXXXXXXXXXLEVDGIDKLDIE 454
+K DDC ++ LV LEV +DK +IE
Sbjct: 311 FKTEDDCGLRANAFIELVAETVYASGALDQLLEVQKLDKYNIE 353
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,727,230
Number of Sequences: 28952
Number of extensions: 161090
Number of successful extensions: 420
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 420
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 838967680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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