Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_A14
(611 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g24230.1 68418.m02851 expressed protein 32 0.26
At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family prote... 31 0.60
At5g67040.1 68418.m08452 hypothetical protein contains Pfam prof... 30 1.4
At1g10040.1 68414.m01132 expressed protein non-consensus GC dono... 30 1.4
At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase (X... 29 3.2
At5g65960.1 68418.m08303 expressed protein 28 5.6
At5g24220.1 68418.m02850 lipase class 3-related 28 5.6
At4g10550.1 68417.m01727 subtilase family protein contains simil... 28 5.6
At4g10260.1 68417.m01684 pfkB-type carbohydrate kinase family pr... 28 5.6
At5g59400.2 68418.m07444 expressed protein predicted protein, Ar... 27 7.4
At5g59400.1 68418.m07443 expressed protein predicted protein, Ar... 27 7.4
At4g10955.1 68417.m01782 lipase class 3 family protein contains ... 27 7.4
At1g26120.1 68414.m03188 esterase-related contains similaity to ... 27 7.4
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 27 9.8
At2g34500.1 68415.m04237 cytochrome P450 family protein similar ... 27 9.8
At1g68960.1 68414.m07893 hypothetical protein contains Pfam prof... 27 9.8
At1g44130.1 68414.m05097 nucellin protein, putative similar to n... 27 9.8
At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase f... 27 9.8
>At5g24230.1 68418.m02851 expressed protein
Length = 369
Score = 32.3 bits (70), Expect = 0.26
Identities = 29/87 (33%), Positives = 45/87 (51%)
Frame = +2
Query: 47 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLTGLQVGR 226
LH +T RF+ + + +A+AK G + A+ L GHSLG+ ++ AGK T +G +
Sbjct: 149 LHDST--RFV-HAIQVIQSAVAKTGNA--AVWLAGHSLGAAVALLAGKIMTR-SGFPLES 202
Query: 227 ISGLDPAGPCFSNIPAELRLKHTDAKY 307
P FS+IP E +K K+
Sbjct: 203 YL----FNPPFSSIPIEKLVKSEKLKH 225
>At4g10050.1 68417.m01643 hydrolase, alpha/beta fold family protein
similar to protein phosphatase methylesterase-1 [Homo
sapiens] GI:5533003; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 350
Score = 31.1 bits (67), Expect = 0.60
Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 3/46 (6%)
Frame = +2
Query: 92 EVLAAMAK-GGMSPDAIHLIGHSLGSHIS--GFAGKTFTTLTGLQV 220
+VLA + + G SP AI L+GHS+G ++ A KT +L GL V
Sbjct: 134 DVLAVIKELYGDSPPAIVLVGHSMGGSVAVQVAANKTLPSLAGLVV 179
>At5g67040.1 68418.m08452 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 165
Score = 29.9 bits (64), Expect = 1.4
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 71 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH 169
F+G L A G+ P++I+ IGH LGS+
Sbjct: 104 FLGASEAFCLTASMYPGLKPNSIYYIGHGLGSY 136
>At1g10040.1 68414.m01132 expressed protein non-consensus GC donor
splice site at exon boundary 21576
Length = 412
Score = 29.9 bits (64), Expect = 1.4
Identities = 19/48 (39%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = +2
Query: 50 HST-TY--VRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFA 184
HST T+ V +GE+L E + ++ K S I +GHSLG I+ +A
Sbjct: 120 HSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYA 167
>At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase
(XSP1) identical to subtilisin-type serine endopeptidase
XSP1 GI:6708179 from [Arabidopsis thaliana]
Length = 749
Score = 28.7 bits (61), Expect = 3.2
Identities = 23/94 (24%), Positives = 41/94 (43%), Gaps = 9/94 (9%)
Frame = +2
Query: 92 EVLAAMAKGGMSPDAIHLIGHSLGSHISGFAG-----KTFT----TLTGLQVGRISGLDP 244
+V+ + G++PD+ + H LG + + G K FT + G + + G P
Sbjct: 138 DVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVP 197
Query: 245 AGPCFSNIPAELRLKHTDAKYVDVIHTDGGVYGL 346
AG S I + HT + V+ + +YG+
Sbjct: 198 AGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGI 231
>At5g65960.1 68418.m08303 expressed protein
Length = 393
Score = 27.9 bits (59), Expect = 5.6
Identities = 11/39 (28%), Positives = 20/39 (51%)
Frame = -2
Query: 133 IGTHTALCHSREDFTELLSYEPNVRGRMEVQPTDERAAV 17
+GT C+ ED +L+S N+R + + P +R +
Sbjct: 332 LGTENEKCYEFEDVEQLMSEIGNIRDNLRLMPDFQRREI 370
>At5g24220.1 68418.m02850 lipase class 3-related
Length = 376
Score = 27.9 bits (59), Expect = 5.6
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +2
Query: 125 SPDAIHLIGHSLGSHISGFAGKTFTT 202
S AI L GHSLG+ + AGKT T+
Sbjct: 171 SEAAIWLAGHSLGAALVLLAGKTMTS 196
>At4g10550.1 68417.m01727 subtilase family protein contains
similarity to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana]
Length = 778
Score = 27.9 bits (59), Expect = 5.6
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +2
Query: 74 IGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSH-ISGFAGKTFTTLTGLQVGRISGLDPAG 250
+G+ +G +A + G + A ++ + + +S A T TT + +SG A
Sbjct: 500 VGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQGFIMLSGTSMAA 559
Query: 251 PCFSNIPAELRLKHTD 298
P S + A L+ H D
Sbjct: 560 PAISGVAALLKALHRD 575
>At4g10260.1 68417.m01684 pfkB-type carbohydrate kinase family
protein contains Pfam profile: PF00294 pfkB family
carbohydrate kinase
Length = 324
Score = 27.9 bits (59), Expect = 5.6
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -2
Query: 124 HTALCHSREDFTELLSYEPNVRGRMEVQPTDERAAVQG 11
H A + +D LLSY+PNV R+ + P+ E AA++G
Sbjct: 150 HMAAMKTAKDAGVLLSYDPNV--RLPLWPSTE-AAIEG 184
>At5g59400.2 68418.m07444 expressed protein predicted protein,
Arabidopsis thaliana
Length = 301
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +3
Query: 42 CTSIRPRTFGS*ERSSVKSSRLWQRA 119
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At5g59400.1 68418.m07443 expressed protein predicted protein,
Arabidopsis thaliana
Length = 299
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +3
Query: 42 CTSIRPRTFGS*ERSSVKSSRLWQRA 119
CTSIRPR GS S V+ SRL RA
Sbjct: 5 CTSIRPRLIGS--SSIVELSRLINRA 28
>At4g10955.1 68417.m01782 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 350
Score = 27.5 bits (58), Expect = 7.4
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +2
Query: 47 LHSTTYVRFIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKT 193
LH+TT + + ++A++ GG S + L GHSLG+ ++ GKT
Sbjct: 136 LHTTTRFEIAIQAVRNIVASV--GGSS---VWLAGHSLGASMALLTGKT 179
>At1g26120.1 68414.m03188 esterase-related contains similaity to
esterase 6 GI:606998 from [Drosophila simulans] and
esterase GI:12584120 from [Sphingomonas elodea]
Length = 476
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 107 MAKGGMSPDAIHLIGHSLGSHIS 175
+A+ G PD I+L+G S G+HI+
Sbjct: 270 IAEYGGDPDRIYLMGQSAGAHIA 292
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 27.1 bits (57), Expect = 9.8
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = +2
Query: 71 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT--GLQVGRISGLDP 244
F+ +K+ V A A G++ A+ + G S A K F + GL + LDP
Sbjct: 328 FVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISESE-ATKNFYLIDKDGLVTTERTKLDP 386
Query: 245 AGPCFSNIPAELR 283
F+ PAE+R
Sbjct: 387 GAVLFAKNPAEIR 399
>At2g34500.1 68415.m04237 cytochrome P450 family protein similar to
Cytochrome P450 61 (C-22 sterol desaturase) (SP:P54781)
{Saccharomyces cerevisiae}
Length = 495
Score = 27.1 bits (57), Expect = 9.8
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +2
Query: 128 PDAIHLIGHSLGSHISG 178
PDA HLIGH G + G
Sbjct: 101 PDAFHLIGHPFGKKLFG 117
>At1g68960.1 68414.m07893 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 376
Score = 27.1 bits (57), Expect = 9.8
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 71 FIGEKLGEVLAAMAKGGMSPDAIHLIGHSLGSHISGFAGKTFTTLT 208
F+GE L+A G++P++++ GH G I A T L+
Sbjct: 313 FLGENESFCLSAKDFPGLNPNSVYFAGHGSGYGIWDLASCTIRYLS 358
>At1g44130.1 68414.m05097 nucellin protein, putative similar to
nucellin GI:2290202 from [Hordeum vulgare]
Length = 405
Score = 27.1 bits (57), Expect = 9.8
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = +3
Query: 207 PDFKSAGYRDWIRRDPASVIFPLS 278
P KS+ ++ +I+ P+SV+FPLS
Sbjct: 17 PLSKSSIFKTFIKSSPSSVVFPLS 40
>At1g06520.1 68414.m00691 phospholipid/glycerol acyltransferase
family protein contains Pfam profile PF01553:
Acyltransferase
Length = 585
Score = 27.1 bits (57), Expect = 9.8
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = -1
Query: 194 TSYQRNLICESRGCALSDESHRDSY 120
T YQ L+ ++R C++S + +RD++
Sbjct: 82 TKYQELLLDKNRACSVSSDHYRDTF 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,649,582
Number of Sequences: 28952
Number of extensions: 260878
Number of successful extensions: 655
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 654
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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