Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0003_A07
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g03190.1 68416.m00315 glutathione S-transferase, putative ide... 40 0.001
At5g17220.1 68418.m02018 glutathione S-transferase, putative 38 0.004
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 38 0.006
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 37 0.013
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 33 0.16
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 31 0.50
At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 31 0.50
At1g49660.1 68414.m05569 expressed protein 31 0.50
At4g02520.1 68417.m00345 glutathione S-transferase, putative 30 1.2
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 1.2
At2g02930.1 68415.m00241 glutathione S-transferase, putative 30 1.2
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 30 1.5
At2g20980.1 68415.m02483 expressed protein 30 1.5
At4g31230.1 68417.m04433 protein kinase family protein contains ... 29 3.5
At1g48900.1 68414.m05478 signal recognition particle 54 kDa prot... 29 3.5
At4g11400.1 68417.m01838 ARID/BRIGHT DNA-binding domain-containi... 28 4.7
At3g16830.1 68416.m02149 WD-40 repeat family protein contains 10... 28 4.7
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 28 4.7
At5g25220.1 68418.m02990 homeobox protein knotted-1 like 3 (KNAT... 28 6.1
At3g05360.1 68416.m00584 disease resistance family protein / LRR... 28 6.1
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 28 6.1
At4g35300.2 68417.m05018 transporter-related low similarity to h... 27 8.1
At4g35300.1 68417.m05017 transporter-related low similarity to h... 27 8.1
At3g62900.1 68416.m07066 expressed protein ; expression supporte... 27 8.1
At3g23120.1 68416.m02914 leucine-rich repeat family protein cont... 27 8.1
At2g24370.1 68415.m02912 protein kinase family protein contains ... 27 8.1
At1g14380.2 68414.m01705 calmodulin-binding family protein conta... 27 8.1
At1g14380.1 68414.m01704 calmodulin-binding family protein conta... 27 8.1
>At3g03190.1 68416.m00315 glutathione S-transferase, putative
identical to glutathione S-transferase GB:AAB09584 from
[Arabidopsis thaliana]
Length = 214
Score = 40.3 bits (90), Expect = 0.001
Identities = 30/85 (35%), Positives = 44/85 (51%), Gaps = 6/85 (7%)
Frame = +3
Query: 66 VVFYYFPVKALGEGPRMLLAYGGQ--EFEDKRISMDQWPEIKP----KTPFGQMPLLEID 227
VV Y +KA R+LL + + EFE + +D+ + KP + PFGQ+P +E
Sbjct: 2 VVKVYGQIKAANP-QRVLLCFLEKDIEFEVIHVDLDKLEQKKPQHLLRQPFGQVPAIEDG 60
Query: 228 GKQYAQSIAISRYLGRKYGLAGADL 302
+ +S AI+RY KY G DL
Sbjct: 61 YLKLFESRAIARYYATKYADQGTDL 85
>At5g17220.1 68418.m02018 glutathione S-transferase, putative
Length = 214
Score = 38.3 bits (85), Expect = 0.004
Identities = 25/70 (35%), Positives = 38/70 (54%), Gaps = 6/70 (8%)
Frame = +3
Query: 111 RMLLAY--GGQEFEDKRISMDQWPEIKP----KTPFGQMPLLEIDGKQYAQSIAISRYLG 272
R+LL + G EFE I +D + + KP + PFGQ+P +E + +S AI+RY
Sbjct: 16 RVLLCFLEKGIEFEIIHIDLDTFEQKKPEHLLRQPFGQVPAIEDGDFKLFESRAIARYYA 75
Query: 273 RKYGLAGADL 302
K+ G +L
Sbjct: 76 TKFADQGTNL 85
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 37.9 bits (84), Expect = 0.006
Identities = 25/66 (37%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +3
Query: 174 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYG---LAGADLEEDFEIDQNVEFLN 344
PE P G++P+LE +S AI+RY+ RK G L G+ L E I+Q ++F +
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSS 102
Query: 345 -DIRAN 359
+I AN
Sbjct: 103 LEIDAN 108
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 36.7 bits (81), Expect = 0.013
Identities = 27/84 (32%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Frame = +3
Query: 66 VVFYYFPVKALGEGPRMLLAYGGQEFEDKRISM----DQWPEIKPKTPFGQMPLLEIDGK 233
V+ Y P+ A + + L G FE + + + PE PFG++P+L +DG
Sbjct: 2 VLTIYAPLFASSKRAVVTLVEKGVSFETVNVDLMKGEQRQPEYLAIQPFGKIPVL-VDGD 60
Query: 234 -QYAQSIAISRYLGRKYGLAGADL 302
+ +S AI RY+ KY G DL
Sbjct: 61 YKIFESRAIMRYIAEKYRSQGPDL 84
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 33.1 bits (72), Expect = 0.16
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +3
Query: 174 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYG---LAGADLEEDFEIDQNVEF 338
P P G++P+LE +S AI+RY+ R G L G+ L E +I+Q ++F
Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDF 100
>At3g62760.1 68416.m07050 glutathione S-transferase, putative
Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
Length = 219
Score = 31.5 bits (68), Expect = 0.50
Identities = 15/43 (34%), Positives = 22/43 (51%)
Frame = +3
Query: 174 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 302
P PFG++P L+ D +S AI+ Y+ K+ G DL
Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAITAYIAEKHRDKGTDL 85
>At1g49860.1 68414.m05590 glutathione S-transferase, putative
similar to GI:860955 from [Hyoscyamus muticus] (Plant
Physiol. 109 (1), 253-260 (1995))
Length = 254
Score = 31.5 bits (68), Expect = 0.50
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +3
Query: 195 PFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADLEED 311
PFG++P+LE + + AI+RYL +Y G +L D
Sbjct: 53 PFGEVPVLEDGDLKLFEPKAITRYLAEQYKDVGTNLLPD 91
>At1g49660.1 68414.m05569 expressed protein
Length = 319
Score = 31.5 bits (68), Expect = 0.50
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -2
Query: 578 LFKCRFLKHRLQVIEHTGEDEIAPSQLSKSDVAAVLLEYFIQFL 447
L KC + + ++V+E GED + Q KSD A L+ F++F+
Sbjct: 275 LEKCEW-EGTVEVVEEEGEDHVFHLQNPKSDKALKFLKKFVEFI 317
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 189 KTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 302
+ PFGQ+P E + +S AI++Y+ +Y G +L
Sbjct: 49 RNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNL 86
>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
identical to GB:X95295. Based on identical cDNA hits,
the translation is now 40 AAs longer at the N-terminal,
and start of exon2 is also corrected.
Length = 263
Score = 30.3 bits (65), Expect = 1.2
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +3
Query: 195 PFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 302
PFGQ+P LE +S AI++YL +Y G L
Sbjct: 99 PFGQIPALEDGDLTLFESRAITQYLAEEYSEKGEKL 134
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 189 KTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 302
+ PFGQ+P E + +S AI++Y+ +Y G +L
Sbjct: 49 RNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNL 86
>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
putative similar to SP|Q03460 Glutamate synthase [NADH],
chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
{Medicago sativa}
Length = 2208
Score = 29.9 bits (64), Expect = 1.5
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 5/52 (9%)
Frame = +2
Query: 74 LLFPREGARRGSADVASLRRAGIRR-----QTHINGPMA*NQAQDPVRSNAV 214
L FP G GSA+ ++L G + H+N P+A + Q+ R+N+V
Sbjct: 915 LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSV 966
>At2g20980.1 68415.m02483 expressed protein
Length = 396
Score = 29.9 bits (64), Expect = 1.5
Identities = 27/100 (27%), Positives = 45/100 (45%), Gaps = 4/100 (4%)
Frame = +3
Query: 231 KQYAQSIAISRYLGRKYGLAGADLEEDFEIDQNVEFLNDI-RANAAAVHYEKDEAVK--- 398
K+ Q + + G + L+GAD Q + FLN++ R A+ +K EAV
Sbjct: 291 KKTTQPVRVLSVEGLRKALSGADKVTPNVHSQGIRFLNEMARQKASKNVNKKSEAVNKST 350
Query: 399 AKKLAEFRETKYQFLIEKLNEILQKNGGHIALGKLTWGDF 518
K+ A +ET+ ++ +K I+ GK+ DF
Sbjct: 351 EKRKASTKETQVNGEPKRKKTEHRKETPEISTGKMMLLDF 390
>At4g31230.1 68417.m04433 protein kinase family protein contains
Pfam profiles PF00069: Protein kinase domain, PF00582:
universal stress protein family
Length = 764
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/37 (43%), Positives = 22/37 (59%)
Frame = -2
Query: 500 LSKSDVAAVLLEYFIQFLNEELVFGLAEFCQFLGFHR 390
L +SDVA L+EY Q + E LV G + FL F++
Sbjct: 112 LEESDVARALVEYANQVMIEVLVVGSSSKGGFLRFNK 148
>At1g48900.1 68414.m05478 signal recognition particle 54 kDa protein
3 / SRP54 (SRP-54C) identical to SP|P49967 Signal
recognition particle 54 kDa protein 3 (SRP54)
{Arabidopsis thaliana}
Length = 495
Score = 28.7 bits (61), Expect = 3.5
Identities = 14/47 (29%), Positives = 27/47 (57%)
Frame = +3
Query: 444 IEKLNEILQKNGGHIALGKLTWGDFIFAGMFDYLKSMLQEPTLEEKY 584
++KL E++ K+ L KL+ G+F M+D +++L L+E +
Sbjct: 304 VDKLQEVVPKDQQPELLEKLSQGNFTLRIMYDQFQNILNMGPLKEVF 350
>At4g11400.1 68417.m01838 ARID/BRIGHT DNA-binding domain-containing
protein / ELM2 domain-containing protein / Myb-like
DNA-binding domain-containing protein similar to
BRG1-binding protein ELD/OSA1 [Homo sapiens]
GI:18568414; contains Pfam profiles PF01388: ARID/BRIGHT
DNA binding domain, PF01448: ELM2 domain, PF00249:
Myb-like DNA-binding domain
Length = 573
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = +1
Query: 169 NGLKSSPRPRSVKCRCWKLTVNSTLRASPSAGTSVA 276
+ L S + RS KCR KLT+ ++ RA +AGTS A
Sbjct: 328 SSLVSLSKSRSTKCR--KLTIIASERAGLTAGTSRA 361
>At3g16830.1 68416.m02149 WD-40 repeat family protein contains 10
WD-40 repeats (PF00400) (1 weak)
Length = 1131
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 249 IAISRYLGRKYGLAGADLEEDFEIDQNVEFLNDI 350
++ +++L Y G+DL + EID +V +ND+
Sbjct: 426 VSFTKHLIHVYAYQGSDLRQHLEIDAHVGCVNDL 459
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = +3
Query: 195 PFGQMPLLEIDGK-QYAQSIAISRYLGRKYGLAGADL 302
PFG +P + +DG + +S A+ RY+ KY G DL
Sbjct: 49 PFGTVPAV-VDGDYKIFESRAVMRYVAEKYRSQGPDL 84
>At5g25220.1 68418.m02990 homeobox protein knotted-1 like 3 (KNAT3)
identical to homeobox protein knotted-1 like 3 (KNAT3)
SP:P48000 from [Arabidopsis thaliana]
Length = 431
Score = 27.9 bits (59), Expect = 6.1
Identities = 24/80 (30%), Positives = 35/80 (43%)
Frame = +1
Query: 361 PPPSITRKTRR*KPRNWQNSARPNTSSSLRN*MKYSRRTAATSLLES*LGAISSSPVCSI 540
PPP + + P NW N+A +S + N + TA T+ S S SP S
Sbjct: 31 PPPQQQQHFQEAPPPNWLNTALLRSSDNNNNFLNLHTATANTTTASS-----SDSP--SS 83
Query: 541 T*SRCFRNRHLKRSTRSLRR 600
+ N+ L RS+ L+R
Sbjct: 84 AAAAAAANQWLSRSSSFLQR 103
>At3g05360.1 68416.m00584 disease resistance family protein / LRR
family protein contains leucine rich-repeat domains
Pfam:PF00560, INTERPRO:IPR001611; similar to
elicitor-inducible LRR receptor-like protein EILP
[Nicotiana tabacum] gi|6635236|dbj|BAA88636; similar to
Cf-2.2 [Lycopersicon pimpinellifolium]
gi|1184077|gb|AAC15780
Length = 786
Score = 27.9 bits (59), Expect = 6.1
Identities = 21/62 (33%), Positives = 29/62 (46%)
Frame = -2
Query: 389 LVFLVMDGGGVCTNIVKELHVLINLEVFL*VCTSEAVLATEVPADGDALSVLFTVNFQQR 210
L L + G +NI + L L NLE + S L+ +P D +LS L T+NF
Sbjct: 624 LRLLNLSGNSFTSNIPQSLANLTNLET---LDLSRNQLSGHIPRDLGSLSFLSTMNFSHN 680
Query: 209 HL 204
L
Sbjct: 681 LL 682
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 27.9 bits (59), Expect = 6.1
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +3
Query: 174 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 302
P PFGQ+P+ G + +S AIS Y+ + G L
Sbjct: 79 PSFLAINPFGQVPVFLDGGLKLTESRAISEYIATVHKSRGTQL 121
>At4g35300.2 68417.m05018 transporter-related low similarity to
hexose transporter [Solanum tuberosum] GI:8347246;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 729
Score = 27.5 bits (58), Expect = 8.1
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 51 LTMPNVVFYYFPVKALGEGPRMLLAYGGQEFEDKRI 158
L +P++VF++ V L E PR L++ G+ E KR+
Sbjct: 170 LFIPSLVFFFLTVFFLPESPRWLVS-KGRMLEAKRV 204
>At4g35300.1 68417.m05017 transporter-related low similarity to
hexose transporter [Solanum tuberosum] GI:8347246;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 739
Score = 27.5 bits (58), Expect = 8.1
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 51 LTMPNVVFYYFPVKALGEGPRMLLAYGGQEFEDKRI 158
L +P++VF++ V L E PR L++ G+ E KR+
Sbjct: 170 LFIPSLVFFFLTVFFLPESPRWLVS-KGRMLEAKRV 204
>At3g62900.1 68416.m07066 expressed protein ; expression supported by
MPSS
Length = 1401
Score = 27.5 bits (58), Expect = 8.1
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -1
Query: 144 RIPARRRLATSADPRRAPSRG 82
RIP R +A A P R PSRG
Sbjct: 1087 RIPERSNIADLASPSRPPSRG 1107
>At3g23120.1 68416.m02914 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Hcr2-5b GB:AAC78595
[Lycopersicon esculentum] (Plant Cell 10, 1915-1926
(1998);
Length = 784
Score = 27.5 bits (58), Expect = 8.1
Identities = 20/62 (32%), Positives = 27/62 (43%)
Frame = -2
Query: 389 LVFLVMDGGGVCTNIVKELHVLINLEVFL*VCTSEAVLATEVPADGDALSVLFTVNFQQR 210
L+ L + G NI L + NLE + S L+ E+P LS L +NF
Sbjct: 635 LLHLNLSGNAFTGNIPPSLANITNLET---LDLSRNNLSGEIPRSLGNLSFLSNINFSHN 691
Query: 209 HL 204
HL
Sbjct: 692 HL 693
>At2g24370.1 68415.m02912 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 816
Score = 27.5 bits (58), Expect = 8.1
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -2
Query: 500 LSKSDVAAVLLEYFIQFLNEELVFGLAEFCQFLGFHR 390
L +SDVA L+EY Q E LV G + FL F++
Sbjct: 111 LEESDVAKALVEYVNQAAIEVLVVGSSSKGGFLRFNK 147
>At1g14380.2 68414.m01705 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 602
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/63 (23%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +3
Query: 57 MPNVVFYYFPVKALGEGPRML---LAYGGQEFEDKRISMDQWPEIKPKTPFGQMPLLEID 227
M N + F K L P L + YG +E ++ +++W +++ + ++P +EI
Sbjct: 187 MDNPTKFVFVDKLLASSPTALPLKIQYGPEEPNSAKVWLERWTQLQVWSSGSRVPRIEIP 246
Query: 228 GKQ 236
Q
Sbjct: 247 KSQ 249
>At1g14380.1 68414.m01704 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 664
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/63 (23%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +3
Query: 57 MPNVVFYYFPVKALGEGPRML---LAYGGQEFEDKRISMDQWPEIKPKTPFGQMPLLEID 227
M N + F K L P L + YG +E ++ +++W +++ + ++P +EI
Sbjct: 187 MDNPTKFVFVDKLLASSPTALPLKIQYGPEEPNSAKVWLERWTQLQVWSSGSRVPRIEIP 246
Query: 228 GKQ 236
Q
Sbjct: 247 KSQ 249
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,404,493
Number of Sequences: 28952
Number of extensions: 278077
Number of successful extensions: 811
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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