Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_P19
(524 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 123 1e-29
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 112 3e-26
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 92 4e-20
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 83 3e-17
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 70 2e-13
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 32 0.060
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 27 1.7
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 25 5.2
SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G family|Schizosaccha... 25 6.9
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos... 25 6.9
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 123 bits (297), Expect = 1e-29
Identities = 63/160 (39%), Positives = 87/160 (54%)
Frame = +3
Query: 45 KVDIKYTKLFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRY 224
K I+ LFINN+ VD+V +P E +I +VA+ F
Sbjct: 17 KTYIQPVGLFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTD 76
Query: 225 SEWRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAG 404
+ WR ++QRG L +LA +E++ +YLA +ETLDNGK + A +V A+ RYY G
Sbjct: 77 APWRKFSSAQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGG 136
Query: 405 KADKILGNTIPADGEVLTFTLKEPVGVCGQILPWNYPIPM 524
ADK G TI D + +T EP+GVCGQI+PWN+P M
Sbjct: 137 WADKDYGQTIETDIKRFAYTRHEPIGVCGQIIPWNFPFLM 176
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 112 bits (270), Expect = 3e-26
Identities = 69/174 (39%), Positives = 92/174 (52%), Gaps = 3/174 (1%)
Frame = +3
Query: 12 LFTRVNFI--KMVKVDIKYTKLFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXX 185
LF +NF K VK I L+IN EW S +T+ T++P E VI +V
Sbjct: 5 LFVSINFPNGKSVKQPIG---LYINGEWHK--SAETWETVDPSIEEVIAKVYLAGEKEID 59
Query: 186 XXXXXXXXXFHRYSEWRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKP-VKQAEQ 362
F W+ + S++G LL KLA L E+ A LA +E +D+GKP V A
Sbjct: 60 YAVKSAKEAF---KTWKKVPGSEKGELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARG 116
Query: 363 EVLWASGIVRYYAGKADKILGNTIPADGEVLTFTLKEPVGVCGQILPWNYPIPM 524
+V ++RY AG ADKI G IP E L + + P+GVCGQI+PWNYP+ M
Sbjct: 117 DVDGTIALLRYCAGWADKIYGQVIPTGPEKLAYAKRTPIGVCGQIVPWNYPLNM 170
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 92.3 bits (219), Expect = 4e-20
Identities = 50/160 (31%), Positives = 81/160 (50%), Gaps = 2/160 (1%)
Frame = +3
Query: 51 DIKYTKLFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSE 230
D + + F+ +W+ + + KTF NP +I +VA+ F Y
Sbjct: 14 DKSHAQSFVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKN 73
Query: 231 WRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKA 410
+ + SQ LL + A L+ + L ++ TL+NGKP+ QAE EV SG +++YA +A
Sbjct: 74 FTHVQRSQ---LLERWAELIMENKDDLVKMLTLENGKPLSQAEMEVTTCSGYLKWYAAEA 130
Query: 411 DKILGNTIPADGEVLTF--TLKEPVGVCGQILPWNYPIPM 524
+ G+ P+ + F ++K+PVGV I PWN+P M
Sbjct: 131 VRTFGDVAPSSLQSQNFLISIKQPVGVSALITPWNFPAAM 170
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 82.6 bits (195), Expect = 3e-17
Identities = 50/174 (28%), Positives = 88/174 (50%), Gaps = 1/174 (0%)
Frame = +3
Query: 6 ARLFTRVNFIKMVKVDIKYTKLFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXX 185
++L T + + + ++ + +I +WV A S KTF NP + V +
Sbjct: 5 SKLSTNIKDLSLFDLEEFPARSYIGGKWVTAASGKTFDVENPGLNETLAPVTDMSVEETR 64
Query: 186 XXXXXXXXXFHRYSEWRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQE 365
F Y R D +R +L + L+ +A LA + TL+NGK + A+ E
Sbjct: 65 KAIKVAHEAFLSY---RNSDIKERYAILRRWYDLIMENADDLATMMTLENGKALGDAKGE 121
Query: 366 VLWASGIVRYYAGKADKILG-NTIPADGEVLTFTLKEPVGVCGQILPWNYPIPM 524
V++A+ + ++AG+A +I G +++ ++ + T+K+PVGV G I PWN+P M
Sbjct: 122 VVYAAKFIDWFAGEALRISGDSSMSSNPQNRIITIKQPVGVVGIITPWNFPAAM 175
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 69.7 bits (163), Expect = 2e-13
Identities = 46/157 (29%), Positives = 71/157 (45%), Gaps = 5/157 (3%)
Frame = +3
Query: 69 LFINNEWVDAV--SKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLL 242
LFI+ ++V + + K P INP E +I A F R W
Sbjct: 23 LFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTF-RSGIWAKW 81
Query: 243 DASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKAD--- 413
QRGL+L K+A +M + LA ++T++ GKP A ++ + + YYA A+
Sbjct: 82 PGKQRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALFDIDSCADMFEYYAEVAETDN 141
Query: 414 KILGNTIPADGEVLTFTLKEPVGVCGQILPWNYPIPM 524
+ +P + F + P GV G I PWN+P+ M
Sbjct: 142 PTVKVPLPNNPGFCAFEKRFPRGVIGVITPWNFPLKM 178
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 31.9 bits (69), Expect = 0.060
Identities = 27/103 (26%), Positives = 44/103 (42%), Gaps = 6/103 (5%)
Frame = +3
Query: 228 EWRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKP-VKQAEQEVLWASGIVRYYAG 404
EW+ ++R L L + R+ AE+ D GK V A E+L + +
Sbjct: 40 EWKSTSFAERRNFLKALKENIIRNQDKYAEIACKDTGKTLVDAAFGEILVTLEKINWTLA 99
Query: 405 KADKILGNTIPADGEVLT-----FTLKEPVGVCGQILPWNYPI 518
++ L T +LT + EP+GV ++ WNYP+
Sbjct: 100 NGEQSLRPT-KRPNSLLTSYKGGYVKYEPLGVIAALVSWNYPL 141
>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 695
Score = 27.1 bits (57), Expect = 1.7
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +3
Query: 261 LLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNTIPA 440
L+ KL + KY ++ D G P+ + + E L SG+ + +K+L +
Sbjct: 531 LIKEKLTVNVPVKPKYSSQETAFDTGAPISEEQIEELLNSGLDEQ---EGEKLLVLHVSE 587
Query: 441 DGEVLTFT--LKEPVGV 485
V TFT LK P G+
Sbjct: 588 KDPVGTFTEVLKNPFGL 604
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 25.4 bits (53), Expect = 5.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 345 VKQAEQEVLWASGIVRYYAGKADKILG 425
VK+ + +W G + YAG+ +ILG
Sbjct: 219 VKKGDTVAIWGMGPIGLYAGRWAQILG 245
>SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 496
Score = 25.0 bits (52), Expect = 6.9
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = +3
Query: 249 SQRGLLLFKLATLMERDAKYLAE--LETLDNGKPVK 350
SQ G LLF L +L ERD + A+ E L+N V+
Sbjct: 143 SQIGKLLFDLRSLNERDNPFHAQNNAENLENATFVR 178
>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 467
Score = 25.0 bits (52), Expect = 6.9
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -1
Query: 356 CLLHWFSVVQCFEF 315
CL+HWF +++ EF
Sbjct: 337 CLVHWFQIMRSAEF 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,061,788
Number of Sequences: 5004
Number of extensions: 38280
Number of successful extensions: 94
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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