BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_P08 (493 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g44222.1 68414.m05107 hypothetical protein 35 0.026 At3g05220.2 68416.m00570 heavy-metal-associated domain-containin... 34 0.045 At3g05220.1 68416.m00569 heavy-metal-associated domain-containin... 34 0.045 At4g28520.3 68417.m04081 12S seed storage protein, putative / cr... 33 0.14 At4g28520.2 68417.m04079 12S seed storage protein, putative / cr... 33 0.14 At4g28520.1 68417.m04080 12S seed storage protein, putative / cr... 33 0.14 At5g28480.1 68418.m03462 hypothetical protein 31 0.42 At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 31 0.42 At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 31 0.42 At2g37410.2 68415.m04588 mitochondrial import inner membrane tra... 30 0.74 At2g37410.1 68415.m04587 mitochondrial import inner membrane tra... 30 0.74 At1g64450.1 68414.m07306 proline-rich family protein contains pr... 30 0.97 At2g42520.1 68415.m05262 DEAD box RNA helicase, putative similar... 27 5.2 At3g03090.1 68416.m00305 sugar transporter family protein simila... 27 6.9 At2g26590.1 68415.m03190 adhesion regulating molecule family sim... 27 6.9 At1g70650.1 68414.m08145 zinc finger (Ran-binding) family protei... 27 9.1 At1g15830.1 68414.m01900 expressed protein 27 9.1 >At1g44222.1 68414.m05107 hypothetical protein Length = 85 Score = 35.1 bits (77), Expect = 0.026 Identities = 17/41 (41%), Positives = 22/41 (53%) Frame = +2 Query: 338 IGMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQG 460 +G PG G G+ G G G G +G +GI G G+ GI G Sbjct: 5 LGRPGMLGSPGMLGILGMFGISGMVGRLGILGRLGMMGIPG 45 Score = 28.3 bits (60), Expect = 3.0 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = +1 Query: 73 GERGWPGIEGLKGEQGENGEFGLDGFTGPVGL 168 G G PG+ G G G G FG+ G G +G+ Sbjct: 3 GRLGRPGMLGSPGMLGILGMFGISGMVGRLGI 34 >At3g05220.2 68416.m00570 heavy-metal-associated domain-containing protein similar to farnesylated protein 1 (GI:23304411) {Hordeum vulgare subsp. spontaneum}; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 478 Score = 34.3 bits (75), Expect = 0.045 Identities = 16/38 (42%), Positives = 19/38 (50%) Frame = +2 Query: 350 GKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G GP G G G +G GPMG +G G + G GP Sbjct: 325 GPMGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGP 362 Score = 31.1 bits (67), Expect = 0.42 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 338 IGMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQG 460 +G G GP G G+ G +G +GP G + + G G G G Sbjct: 327 MGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGPMGPMG 367 Score = 27.1 bits (57), Expect = 6.9 Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 2/37 (5%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIG--IPGARGL 445 G G GP G G G +GPMG G P +GL Sbjct: 343 GPMGMMGPGGPMSMMGPGGPMGPMGGQGGSYPAVQGL 379 >At3g05220.1 68416.m00569 heavy-metal-associated domain-containing protein similar to farnesylated protein 1 (GI:23304411) {Hordeum vulgare subsp. spontaneum}; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 577 Score = 34.3 bits (75), Expect = 0.045 Identities = 16/38 (42%), Positives = 19/38 (50%) Frame = +2 Query: 350 GKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G GP G G G +G GPMG +G G + G GP Sbjct: 424 GPMGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGP 461 Score = 31.1 bits (67), Expect = 0.42 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 338 IGMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQG 460 +G G GP G G+ G +G +GP G + + G G G G Sbjct: 426 MGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGPMGPMG 466 Score = 27.1 bits (57), Expect = 6.9 Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 2/37 (5%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIG--IPGARGL 445 G G GP G G G +GPMG G P +GL Sbjct: 442 GPMGMMGPGGPMSMMGPGGPMGPMGGQGGSYPAVQGL 478 >At4g28520.3 68417.m04081 12S seed storage protein, putative / cruciferin, putative strong similarity to SP|P33525 Cruciferin CRU1 precursor (11S globulin) (12S storage protein) from Brassica napus; contains Pfam profile PF00190 Cupin and Prosite 11-S plant seed storage proteins signature PS00305 isoform contains non-consensus AC acceptor splice site at intron 3 Length = 453 Score = 32.7 bits (71), Expect = 0.14 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Frame = +1 Query: 13 KGYRGDLGEPGIAISPKGQRGERG--WPGIEGLKGEQGENGEFG 138 +G +G G+P +GQ+G +G W G +G +G+QG G+ G Sbjct: 133 QGRQGQQGQPWEGQGQQGQQGRQGQPWEG-QGQQGQQGRQGQQG 175 Score = 28.3 bits (60), Expect = 3.0 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPG--IEGLKGEQGENG 129 G +G +G G+P +GQ+G +G G EG +G+QG+ G Sbjct: 147 GQQGQQGRQGQPWEGQGQQGQQGRQGQQGQPWEG-QGQQGQQG 188 >At4g28520.2 68417.m04079 12S seed storage protein, putative / cruciferin, putative strong similarity to SP|P33525 Cruciferin CRU1 precursor (11S globulin) (12S storage protein) from Brassica napus; contains Pfam profile PF00190 Cupin and Prosite 11-S plant seed storage proteins signature PS00305 isoform contains non-consensus AC acceptor splice site at intron 3 Length = 394 Score = 32.7 bits (71), Expect = 0.14 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Frame = +1 Query: 13 KGYRGDLGEPGIAISPKGQRGERG--WPGIEGLKGEQGENGEFG 138 +G +G G+P +GQ+G +G W G +G +G+QG G+ G Sbjct: 133 QGRQGQQGQPWEGQGQQGQQGRQGQPWEG-QGQQGQQGRQGQQG 175 Score = 28.3 bits (60), Expect = 3.0 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPG--IEGLKGEQGENG 129 G +G +G G+P +GQ+G +G G EG +G+QG+ G Sbjct: 147 GQQGQQGRQGQPWEGQGQQGQQGRQGQQGQPWEG-QGQQGQQG 188 >At4g28520.1 68417.m04080 12S seed storage protein, putative / cruciferin, putative strong similarity to SP|P33525 Cruciferin CRU1 precursor (11S globulin) (12S storage protein) from Brassica napus; contains Pfam profile PF00190 Cupin and Prosite 11-S plant seed storage proteins signature PS00305 isoform contains non-consensus AC acceptor splice site at intron 3 Length = 524 Score = 32.7 bits (71), Expect = 0.14 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Frame = +1 Query: 13 KGYRGDLGEPGIAISPKGQRGERG--WPGIEGLKGEQGENGEFG 138 +G +G G+P +GQ+G +G W G +G +G+QG G+ G Sbjct: 133 QGRQGQQGQPWEGQGQQGQQGRQGQPWEG-QGQQGQQGRQGQQG 175 Score = 28.3 bits (60), Expect = 3.0 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPG--IEGLKGEQGENG 129 G +G +G G+P +GQ+G +G G EG +G+QG+ G Sbjct: 147 GQQGQQGRQGQPWEGQGQQGQQGRQGQQGQPWEG-QGQQGQQG 188 >At5g28480.1 68418.m03462 hypothetical protein Length = 1230 Score = 31.1 bits (67), Expect = 0.42 Identities = 22/53 (41%), Positives = 25/53 (47%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGFTGPVG 165 G G G GE G P G GE G G +G G G +GE G +G G VG Sbjct: 426 GEGGPSGGDGEGG----PSGGDGEGGPSGGDGEGGPNGADGEGGPNGADGEVG 474 Score = 28.7 bits (61), Expect = 2.2 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 426 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPNGADGEGGP 466 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/48 (39%), Positives = 22/48 (45%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGF 150 G G G GE G P G GE G G +G G G +GE G + F Sbjct: 435 GEGGPSGGDGEGG----PSGGDGEGGPNGADGEGGPNGADGEVGDEAF 478 Score = 27.1 bits (57), Expect = 6.9 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 417 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGP 457 >At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28270, At2g05450, At1g45090, At2g16180, At2g06750 Length = 1224 Score = 31.1 bits (67), Expect = 0.42 Identities = 22/53 (41%), Positives = 25/53 (47%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGFTGPVG 165 G G G GE G P G GE G G +G G G +GE G +G G VG Sbjct: 439 GEGGPSGGDGEGG----PSGGDGEGGPSGGDGEGGPNGADGEGGPNGADGEVG 487 Score = 28.7 bits (61), Expect = 2.2 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 439 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPNGADGEGGP 479 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/48 (39%), Positives = 22/48 (45%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGF 150 G G G GE G P G GE G G +G G G +GE G + F Sbjct: 448 GEGGPSGGDGEGG----PSGGDGEGGPNGADGEGGPNGADGEVGDEAF 491 Score = 27.1 bits (57), Expect = 6.9 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 430 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGP 470 >At1g45090.1 68414.m05169 Ulp1 protease family protein similar to At5g28270, At2g12100, At2g05450, At2g16180, At2g06750; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 1210 Score = 31.1 bits (67), Expect = 0.42 Identities = 22/53 (41%), Positives = 25/53 (47%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGFTGPVG 165 G G G GE G P G GE G G +G G G +GE G +G G VG Sbjct: 430 GEGGPSGGDGEGG----PSGGDGEGGPSGGDGEGGPNGADGEGGPNGADGEVG 478 Score = 28.7 bits (61), Expect = 2.2 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 430 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPNGADGEGGP 470 Score = 27.9 bits (59), Expect = 3.9 Identities = 19/48 (39%), Positives = 22/48 (45%) Frame = +1 Query: 7 GLKGYRGDLGEPGIAISPKGQRGERGWPGIEGLKGEQGENGEFGLDGF 150 G G G GE G P G GE G G +G G G +GE G + F Sbjct: 439 GEGGPSGGDGEGG----PSGGDGEGGPNGADGEGGPNGADGEVGDEAF 482 Score = 27.1 bits (57), Expect = 6.9 Identities = 16/41 (39%), Positives = 18/41 (43%) Frame = +2 Query: 341 GMPGKFGPNGIPGKKGEIGDIGPMGSIGIPGARGLQGIQGP 463 G G G +G G G G+ GP G G G G G GP Sbjct: 421 GEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGPSGGDGEGGP 461 >At2g37410.2 68415.m04588 mitochondrial import inner membrane translocase (TIM17) nearly identical to SP|Q9SP35 Mitochondrial import inner membrane translocase subunit TIM17 {Arabidopsis thaliana} Length = 243 Score = 30.3 bits (65), Expect = 0.74 Identities = 13/29 (44%), Positives = 18/29 (62%) Frame = +2 Query: 374 PGKKGEIGDIGPMGSIGIPGARGLQGIQG 460 PG +G G G G G+PG +G+ G+QG Sbjct: 148 PGMQGMPGMQGMQGMPGMPGMQGMPGMQG 176 Score = 29.1 bits (62), Expect = 1.7 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +2 Query: 359 GPNGIPGKKGEIGDIGPMGSIGIPGARGLQ 448 G G+PG +G G G G G+PG +G+Q Sbjct: 149 GMQGMPGMQGMQGMPGMPGMQGMPGMQGMQ 178 >At2g37410.1 68415.m04587 mitochondrial import inner membrane translocase (TIM17) nearly identical to SP|Q9SP35 Mitochondrial import inner membrane translocase subunit TIM17 {Arabidopsis thaliana} Length = 243 Score = 30.3 bits (65), Expect = 0.74 Identities = 13/29 (44%), Positives = 18/29 (62%) Frame = +2 Query: 374 PGKKGEIGDIGPMGSIGIPGARGLQGIQG 460 PG +G G G G G+PG +G+ G+QG Sbjct: 148 PGMQGMPGMQGMQGMPGMPGMQGMPGMQG 176 Score = 29.1 bits (62), Expect = 1.7 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +2 Query: 359 GPNGIPGKKGEIGDIGPMGSIGIPGARGLQ 448 G G+PG +G G G G G+PG +G+Q Sbjct: 149 GMQGMPGMQGMQGMPGMPGMQGMPGMQGMQ 178 >At1g64450.1 68414.m07306 proline-rich family protein contains proline rich extensins, INTERPRO:IPR0002965 Length = 342 Score = 29.9 bits (64), Expect = 0.97 Identities = 14/29 (48%), Positives = 17/29 (58%) Frame = -3 Query: 89 GQPRSPR*PFGLIAIPGSPKSPLYPFNPG 3 G P PR P G PG+P +P+ P NPG Sbjct: 191 GPPSFPRNP-GSPEFPGNPGAPIIPRNPG 218 >At2g42520.1 68415.m05262 DEAD box RNA helicase, putative similar to SP|O00571 DEAD-box protein 3 (Helicase-like protein 2) {Homo sapiens}, DEAD box RNA helicase DDX3 [Homo sapiens] GI:3523150; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 633 Score = 27.5 bits (58), Expect = 5.2 Identities = 14/29 (48%), Positives = 14/29 (48%) Frame = +1 Query: 16 GYRGDLGEPGIAISPKGQRGERGWPGIEG 102 GYR D G PG S G RG GW G Sbjct: 79 GYRADAGRPGSG-SGYGGRGGGGWNNRSG 106 >At3g03090.1 68416.m00305 sugar transporter family protein similar to xylose permease [Bacillus megaterium] GI:1924928; contains Pfam profile PF00083: major facilitator superfamily protein Length = 503 Score = 27.1 bits (57), Expect = 6.9 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = +2 Query: 242 KEIKERAYFHLIFYPASVVKPVFQVLMAGQDFIGM 346 KE+ A+ +L+ +VV PVF +L+ G+ GM Sbjct: 122 KELILAAFLYLVGAIVTVVAPVFSILIIGRVTYGM 156 >At2g26590.1 68415.m03190 adhesion regulating molecule family similar to oocyte membrane protein (GI:6174842) [Xenopus laevis]; similar to Adhesion regulating molecule 1 precursor (110 kDa cell membrane glycoprotein) (Gp110) (Swiss-Prot:Q16186) [Homo sapiens]; contains Pfam PF04683: Adhesion regulating molecule conserved region Length = 300 Score = 27.1 bits (57), Expect = 6.9 Identities = 13/21 (61%), Positives = 13/21 (61%) Frame = +1 Query: 229 PKGIKGDQGEGLLPSDILPGE 291 P GI GDQ EGL DIL E Sbjct: 188 PVGIAGDQDEGLALGDILKPE 208 >At1g70650.1 68414.m08145 zinc finger (Ran-binding) family protein contains Pfam domain, PF00641: Zn-finger in Ran binding protein and others Length = 466 Score = 26.6 bits (56), Expect = 9.1 Identities = 17/42 (40%), Positives = 20/42 (47%) Frame = +1 Query: 289 ERGETGFPGLDGRPGLYWNARKVWS*RHPWKKR*NRRYRSNG 414 E G GFP GR + +A K R WK +R RSNG Sbjct: 382 EDGFMGFPVEGGRSNVSKSAEK----REQWKLEMTQRIRSNG 419 >At1g15830.1 68414.m01900 expressed protein Length = 483 Score = 26.6 bits (56), Expect = 9.1 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 1/47 (2%) Frame = +1 Query: 22 RGDLGEPGIAISPKGQRGERGWPGIEGL-KGEQGENGEFGLDGFTGP 159 RG GEP I +P +RG G P I G ++G GE + G P Sbjct: 127 RGGGGEPAIPGAPPPKRGGGGEPVIPGAPPPKRGGGGEPVIPGAPPP 173 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,015,213 Number of Sequences: 28952 Number of extensions: 160100 Number of successful extensions: 503 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 414 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 485 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 858708096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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