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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_P05
         (571 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g47930.1 68418.m05921 40S ribosomal protein S27 (RPS27D)            93   2e-19
At3g61110.1 68416.m06839 40S ribosomal protein S27 (ARS27A) iden...    87   1e-17
At2g45710.1 68415.m05685 40S ribosomal protein S27 (RPS27A)            85   2e-17
At5g59990.1 68418.m07523 expressed protein ; expression supporte...    32   0.31 
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro...    28   3.8  
At5g03450.1 68418.m00300 zinc finger (C3HC4-type RING finger) fa...    28   5.0  
At2g33350.1 68415.m04088 hypothetical protein                          28   5.0  
At1g51310.1 68414.m05770 tRNA methyl transferase family protein ...    28   5.0  
At5g07410.1 68418.m00848 pectinesterase family protein contains ...    27   8.8  
At1g69940.1 68414.m08049 pectinesterase family protein contains ...    27   8.8  

>At5g47930.1 68418.m05921 40S ribosomal protein S27 (RPS27D)
          Length = 84

 Score = 92.7 bits (220), Expect = 2e-19
 Identities = 40/52 (76%), Positives = 44/52 (84%)
 Frame = +3

Query: 408 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVC 563
           IDLLHP P  E+RKHKLKRLV  PNS+FMDVKC GC+ ITTVFSH+Q VVVC
Sbjct: 7   IDLLHPPPELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58


>At3g61110.1 68416.m06839 40S ribosomal protein S27 (ARS27A)
           identical to cDNA ribosomal protein S27 (ARS27A)
           GI:4193381
          Length = 86

 Score = 86.6 bits (205), Expect = 1e-17
 Identities = 38/52 (73%), Positives = 43/52 (82%)
 Frame = +3

Query: 408 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVC 563
           IDLL+P    E+RKHKLKRLV  PNS+FMDVKC GC+ ITTVFSH+Q VVVC
Sbjct: 7   IDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58


>At2g45710.1 68415.m05685 40S ribosomal protein S27 (RPS27A)
          Length = 84

 Score = 85.4 bits (202), Expect = 2e-17
 Identities = 37/52 (71%), Positives = 43/52 (82%)
 Frame = +3

Query: 408 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVC 563
           IDLL+P    E+RKHKLKRLV  PNS+FMDVKC GC+ ITTVFSH+Q VV+C
Sbjct: 7   IDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58


>At5g59990.1 68418.m07523 expressed protein ; expression supported
           by MPSS
          Length = 241

 Score = 31.9 bits (69), Expect = 0.31
 Identities = 17/44 (38%), Positives = 23/44 (52%)
 Frame = +2

Query: 233 KPEPERTRRKISLRSSSLNIVNFNKRNIELRFICKHHTRGSRPR 364
           K  PE  + KI    S  N+ NFNKR   +++ C+     SRPR
Sbjct: 157 KYSPEEKKEKIEKYRSKRNLRNFNKR---IKYECRKTLADSRPR 197


>At1g31810.1 68414.m03904 formin homology 2 domain-containing protein
            / FH2 domain-containing protein low similarity to
            SP|P48608 Diaphanous protein {Drosophila melanogaster};
            contains Pfam profile PF02181: Formin Homology 2(FH2)
            Domain
          Length = 1201

 Score = 28.3 bits (60), Expect = 3.8
 Identities = 14/65 (21%), Positives = 32/65 (49%)
 Frame = +2

Query: 152  PFLMLRPIFSIFLKTFISAQAQPKLSTKPEPERTRRKISLRSSSLNIVNFNKRNIELRFI 331
            PF  +  I ++F+KTFI ++ + +   + E ++  ++     S+      +  N  ++ I
Sbjct: 1137 PFEQVTKILTLFMKTFIKSREENEKQAEAEKKKLEKEAIKEKSATKKDGVDNDNDLIQQI 1196

Query: 332  CKHHT 346
             +H T
Sbjct: 1197 HRHRT 1201


>At5g03450.1 68418.m00300 zinc finger (C3HC4-type RING finger)
           family protein 
          Length = 630

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = -3

Query: 404 ERHGYSFASCRIRHEGDFLVCG 339
           E HG S  SC  RH+GD L+ G
Sbjct: 266 EHHGQS-PSCSFRHQGDLLING 286


>At2g33350.1 68415.m04088 hypothetical protein
          Length = 440

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 15/44 (34%), Positives = 22/44 (50%)
 Frame = +2

Query: 233 KPEPERTRRKISLRSSSLNIVNFNKRNIELRFICKHHTRGSRPR 364
           K  PE+ + KI       N  NFNK+   +++ C+     SRPR
Sbjct: 335 KLSPEQRKEKIRRYMKKRNERNFNKK---IKYACRKTLADSRPR 375


>At1g51310.1 68414.m05770 tRNA methyl transferase family protein
           contains Pfam PF03054: tRNA methyl transferase profile;
           similar to tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           (EC 2.1.1.61). (Swiss-Prot:Q8ZPZ4) [Salmonella
           typhimurium]
          Length = 421

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = -3

Query: 428 GRVQ*INCE-RHGYSFASCRIRHEGDFLVCGVYI 330
           G+V+ + C+ RHG  F SC    EGD  V  V++
Sbjct: 303 GKVRELRCKVRHGPGFYSCSFEMEGDGDVAVVHL 336


>At5g07410.1 68418.m00848 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 361

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 5/51 (9%)
 Frame = +1

Query: 82  ITINLS*RFSFA*NCDRWSSLDNAVSDVATHFFN--FLE---DFHFGSGAA 219
           +++ +S  F+   NC  +   D    D   HFF   ++E   DF FGSG +
Sbjct: 176 LSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTS 226


>At1g69940.1 68414.m08049 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 361

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 5/51 (9%)
 Frame = +1

Query: 82  ITINLS*RFSFA*NCDRWSSLDNAVSDVATHFFN--FLE---DFHFGSGAA 219
           +++ +S  F+   NC  +   D    D   HFF   ++E   DF FGSG +
Sbjct: 176 LSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTS 226


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,193,924
Number of Sequences: 28952
Number of extensions: 223431
Number of successful extensions: 660
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 660
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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