Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_O06
(510 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 29 0.31
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.53
SPAC2F3.11 |||exopolyphosphatase |Schizosaccharomyces pombe|chr ... 27 1.6
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 26 2.9
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.8
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 3.8
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 25 5.0
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 6.6
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 6.6
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 25 8.7
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 8.7
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 25 8.7
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 25 8.7
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 8.7
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 29.5 bits (63), Expect = 0.31
Identities = 13/42 (30%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = -3
Query: 400 SACIGGTPEISPFGFELPRTSSGADTKDKF-VGRRTILNPVI 278
+A + GTP + P FE P+ S A +F +G +++P +
Sbjct: 770 TASVDGTPMVRPLFFEFPKQISLASVDKQFMIGTALLISPAL 811
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.53
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -2
Query: 248 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 168
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPAC2F3.11 |||exopolyphosphatase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 384
Score = 27.1 bits (57), Expect = 1.6
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +3
Query: 336 LDVRGSSNPNGEISGVPPIHAD*GGEACSYYKGPRLIKGYPEIC 467
LD + N NG I+G+ H D GG S + PR+I+ C
Sbjct: 120 LDRKDLENFNGSIAGIIDHHKDEGG---SLHADPRIIEECGSCC 160
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 26.2 bits (55), Expect = 2.9
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 385 GTPEISPFGFELPRTSSGADTKDKF-VGRRTILNPVIYSSV 266
G P I P FE P S A+ +F VG ++ PV+ +V
Sbjct: 772 GLPLIRPLFFEFPNEPSLANADRQFMVGSALLVTPVLEPNV 812
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.8 bits (54), Expect = 3.8
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -1
Query: 78 CILGFTLSACFISRTRAVKPS 16
CI GF ++ + +RTR + PS
Sbjct: 325 CIAGFVVNELYTTRTRIIAPS 345
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 3.8
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 160 DNVDQVYDAWDVKVNGAIYVWQ 95
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.4 bits (53), Expect = 5.0
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -1
Query: 78 CILGFTLSACFISRTRAVKPS 16
CI GF ++ + +RTR + PS
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS 345
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 6.6
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +2
Query: 119 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 214
Y D P I+ V +N+ D T + NP + D
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 25.0 bits (52), Expect = 6.6
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +1
Query: 325 YQHHWTYVEARIQTVKFPEFHRYTPIRV 408
Y H+ V +R T FP + R P+R+
Sbjct: 272 YVSHFALVHSRFSTNTFPSWDRAQPMRL 299
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 24.6 bits (51), Expect = 8.7
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 5/66 (7%)
Frame = +2
Query: 2 ESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIVNI 166
ES H EG F + E MK+ALN ++ +S ++ I FD I D V
Sbjct: 987 ESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSVED 1046
Query: 167 QAFDYY 184
++Y
Sbjct: 1047 ILTEFY 1052
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 24.6 bits (51), Expect = 8.7
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 191 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 310
+R+P + Y + P N +NA A+NY+ + A H
Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489
>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
L32|Schizosaccharomyces pombe|chr 2|||Manual
Length = 103
Score = 24.6 bits (51), Expect = 8.7
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 29 ALVREMKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINL 148
AL+R A++ K S LP+++ I+ PSI N+
Sbjct: 2 ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 24.6 bits (51), Expect = 8.7
Identities = 16/58 (27%), Positives = 22/58 (37%)
Frame = +3
Query: 315 LSLVSAPLDVRGSSNPNGEISGVPPIHAD*GGEACSYYKGPRLIKGYPEICAKFINPT 488
+SLV G I G P ++ G+ S Y GPR + K + PT
Sbjct: 73 ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPT 130
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 24.6 bits (51), Expect = 8.7
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +2
Query: 179 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 307
Y TP Y P Y P R+P Q+ A + NG PT
Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,200,948
Number of Sequences: 5004
Number of extensions: 46691
Number of successful extensions: 147
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 204242806
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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