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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_N21
         (443 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77270.1 68414.m08999 expressed protein                             31   0.26 
At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type f...    29   1.9  
At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type f...    29   1.9  
At5g44120.3 68418.m05400 12S seed storage protein (CRA1) nearly ...    27   4.3  
At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit...    27   5.7  
At3g48500.1 68416.m05294 expressed protein                             27   5.7  
At2g39770.1 68415.m04883 GDP-mannose  pyrophosphorylase (GMP1) i...    27   5.7  
At1g15200.1 68414.m01817 protein-protein interaction regulator f...    27   7.5  
At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal hydrolase-r...    26   10.0 

>At1g77270.1 68414.m08999 expressed protein
          Length = 682

 Score = 31.5 bits (68), Expect = 0.26
 Identities = 20/60 (33%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
 Frame = +2

Query: 68  TRYISDRRCANIRRNSAAVVMRVRKKLKTGL-RIRRQDLPQPVPLVLREGQDAQRFENRE 244
           TRY+ D +  N R + +  + R  K+LKT + R     + +  P+V  + +D QR +NRE
Sbjct: 390 TRYVRDSQEKNTRNDESQTIFRASKRLKTEIGRADHNSISKTNPVV--KTKDIQR-KNRE 446


>At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type
           family protein contains Pfam domain PF00050: Kazal-type
           serine protease inhibitor domain
          Length = 144

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 18/49 (36%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
 Frame = +3

Query: 120 PSSCA--CARNLRPVCGSDGKTYHNQCLLYCERDKTHSDLKIVKEGTCE 260
           P+SC   C R   PVCG D  TY   C      D     +++VK+G C+
Sbjct: 68  PASCPVQCFRP-DPVCGEDSVTYWCGC-----ADALCHGVRVVKQGACD 110


>At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type
           family protein contains Pfam domain PF00050: Kazal-type
           serine protease inhibitor domain
          Length = 117

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = +3

Query: 153 PVCGSDGKTYHNQCLLYCERDKTHSDLKIVKEGTCE 260
           PVCG+DG TY   C      D      ++VK+G C+
Sbjct: 53  PVCGTDGVTYWCGC-----PDAACHGARVVKKGACD 83


>At5g44120.3 68418.m05400 12S seed storage protein (CRA1) nearly
           identical to SP|P15455 [Plant Mol Biol 11:805-820
           (1988)]; contains Pfam profile PF00190 Cupin and Prosite
           11-S plant seed storage proteins signature PS00305
          Length = 472

 Score = 27.5 bits (58), Expect = 4.3
 Identities = 15/43 (34%), Positives = 21/43 (48%)
 Frame = +3

Query: 291 YAPVCGTDGNTYPNKCSLECSRPLAPSLEMKHRGECHEVKVAD 419
           YA   G  G  +PN+C L+    L PS  +K   E   ++V D
Sbjct: 21  YAAQQGQQGQQFPNECQLDQLNALEPSHVLK--SEAGRIEVWD 61


>At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit
           SKP1/ASK1 (At2) / UFO-binding protein (UIP2) E3
           ubiquitin ligase; skp1b; identical to UIP2 GI:3719211
           from [Arabidopsis thaliana]; contains Pfam profiles
           PF01466: Skp1 family, dimerisation domain and
           PF03931:Skp1 family, tetramerisation domain; identical
           to cDNA UFO binding protein UIP2 mRNA, partial cds
           GI:3719210
          Length = 171

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +1

Query: 118 CRRHARAQET*DRSADQTARLTTTSAS 198
           C+RH  A E  + +AD  A  TTT+ +
Sbjct: 60  CKRHVEAAEKSETTADAAAATTTTTVA 86


>At3g48500.1 68416.m05294 expressed protein 
          Length = 668

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +3

Query: 285 FIYAPVCGTDGNTYPNKCSLECSRPLAP 368
           F + P+   DG+T P++   +C RP  P
Sbjct: 354 FDFPPIFHRDGDTNPDEIRRDCGRPPEP 381


>At2g39770.1 68415.m04883 GDP-mannose  pyrophosphorylase (GMP1)
           identical to GDP-mannose pyrophosphorylase from
           Arabidopsis thaliana [GI:3598958]; updated per Conklin
           PL et al, PNAS 1999, 96(7):4198-203
          Length = 361

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 8/17 (47%), Positives = 13/17 (76%)
 Frame = -2

Query: 442 RVQIHGCISATLTSWHS 392
           R++ H CIS+++  WHS
Sbjct: 297 RIKKHACISSSIIGWHS 313


>At1g15200.1 68414.m01817 protein-protein interaction regulator
           family protein contains Pfam PF04696: pinin/SDK/memA/
           protein conserved region
          Length = 423

 Score = 26.6 bits (56), Expect = 7.5
 Identities = 17/56 (30%), Positives = 29/56 (51%)
 Frame = +2

Query: 80  SDRRCANIRRNSAAVVMRVRKKLKTGLRIRRQDLPQPVPLVLREGQDAQRFENRER 247
           SD++ + + R S +   R  ++ +T  R     LP+P P VL + +D  +  NR R
Sbjct: 112 SDKKQSGLHRGSWS--QRDAEQRRTNKRYEAFALPEPAPRVLPKNED-PKLVNRNR 164


>At1g65200.1 68414.m07392 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1101

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 13/43 (30%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
 Frame = +3

Query: 189 QCLLYCERDKTHSDLKIVKEGTCEEAD--PCVCTFIYAPVCGT 311
           Q + +CE D+ +  L+ +K+  CE  D    VC  + +  C T
Sbjct: 383 QIICFCECDELNQILEFLKKIKCERDDGTDLVCRVVESFYCAT 425


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,513,309
Number of Sequences: 28952
Number of extensions: 195780
Number of successful extensions: 581
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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