BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_N10 (469 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 40 8e-04 At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 39 0.002 At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 31 0.51 At1g50030.1 68414.m05614 target of rapamycin protein (TOR) ident... 30 0.68 At1g36730.1 68414.m04569 eukaryotic translation initiation facto... 30 0.90 At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydro... 29 1.2 At4g01110.1 68417.m00149 hypothetical protein 27 4.8 At1g73200.1 68414.m08471 expressed protein 27 4.8 At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein ... 27 6.3 At5g61390.1 68418.m07702 exonuclease family protein contains exo... 27 8.4 >At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 39.9 bits (89), Expect = 8e-04 Identities = 14/35 (40%), Positives = 24/35 (68%) Frame = +2 Query: 2 SEEVILKWYREPNSSKGKMMFLDQMKKFVEWLQNA 106 +E+ IL W+R+ +SKG+ F+ ++ FV WL+ A Sbjct: 373 AEDTILHWFRKGTNSKGRQTFVKSLEPFVNWLEEA 407 >At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|P47823 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus cuniculus}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 38.7 bits (86), Expect = 0.002 Identities = 14/35 (40%), Positives = 23/35 (65%) Frame = +2 Query: 2 SEEVILKWYREPNSSKGKMMFLDQMKKFVEWLQNA 106 +E+ IL WYR+ + KG+ F+ ++ FV WL+ A Sbjct: 373 AEDTILHWYRKGTNPKGRQTFVKGLEPFVNWLEEA 407 >At1g77840.1 68414.m09070 eukaryotic translation initiation factor 5, putative / eIF-5, putative similar to SP|P55876 Eukaryotic translation initiation factor 5 (eIF-5) {Zea mays}; contains Pfam profiles PF02020: eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in IF2B/IF5 Length = 437 Score = 30.7 bits (66), Expect = 0.51 Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = +2 Query: 5 EEVILKWYREPNSSKGKMMFL-DQMKKFVEWLQNA 106 EEV+L WY + + K + +K FVEWLQ+A Sbjct: 395 EEVVLDWYEKGLTGADKSSPVWKNVKPFVEWLQSA 429 >At1g50030.1 68414.m05614 target of rapamycin protein (TOR) identical to pTOR [Arabidopsis thaliana] GI:12002902; contains Pfam profiles PF00454 Phosphatidylinositol 3- and 4-kinase, PF02259 FAT domain, PF02260 FATC domain Length = 2481 Score = 30.3 bits (65), Expect = 0.68 Identities = 15/58 (25%), Positives = 30/58 (51%) Frame = +2 Query: 230 QTDRQEFKPADGAVNLPEPRRTTPSSIFNNCIIMISDLSRETSEWRRRILKKNPHKYT 403 + D ++ PAD ++LP+P+R+T + M+ D + +E ++ + HK T Sbjct: 2364 EPDEEDEDPAD--IDLPQPQRSTREKEILQAVNMLGDANEVLNERAVVVMARMSHKLT 2419 >At1g36730.1 68414.m04569 eukaryotic translation initiation factor 5, putative / eIF-5, putative similar to SP|P55876 Eukaryotic translation initiation factor 5 (eIF-5) {Zea mays}; contains Pfam profiles PF02020: eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in IF2B/IF5 Length = 439 Score = 29.9 bits (64), Expect = 0.90 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +2 Query: 5 EEVILKWYREPNSSKGKMMFLDQMKKFVEWLQNA 106 E+VI++WY N L + F+EWLQNA Sbjct: 402 EDVIVEWY---NKGVKSSPVLKNVTPFIEWLQNA 432 >At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydroxynaphthoic acid synthetase, putative / DHNA synthetase, putative contains similarity to MENB from Escherichia coli [SP|P27290], Bacillus subtilis [SP|P23966]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 337 Score = 29.5 bits (63), Expect = 1.2 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +2 Query: 317 NCIIMISDLSRETSEWRRRILKKNP 391 N ++ + DL +ET +W R IL+ +P Sbjct: 254 NTVVPLEDLEKETVKWCREILRNSP 278 >At4g01110.1 68417.m00149 hypothetical protein Length = 265 Score = 27.5 bits (58), Expect = 4.8 Identities = 13/51 (25%), Positives = 26/51 (50%), Gaps = 2/51 (3%) Frame = -1 Query: 469 FNRFNELCACVCLSVCVRMCFYCVFMWIFF--*NPPTPFARFSRQITNHYN 323 ++R C CVC++V + + + + +FF +P P R S +++N Sbjct: 58 WSRCRVFCCCVCITVAIVILLLILTVSVFFLYYSPRLPVVRLSSFRVSNFN 108 >At1g73200.1 68414.m08471 expressed protein Length = 779 Score = 27.5 bits (58), Expect = 4.8 Identities = 18/69 (26%), Positives = 31/69 (44%), Gaps = 4/69 (5%) Frame = +2 Query: 224 HRQTDRQEFKPA-DGAVNLPEPRRTTPSSIFNNCIIMISDLSRETSEWRRRILKKNPHKY 400 +R+ DRQE + D +PR++ S+ +I I +L +W + L K K Sbjct: 36 NRKRDRQESNSSSDPNFKSFDPRQSIDFSLNKQGVIWILELDENVKDWMKEKLPKEQKKK 95 Query: 401 TI---KTHP 418 + + HP Sbjct: 96 RVDLLEVHP 104 >At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 521 Score = 27.1 bits (57), Expect = 6.3 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 6/60 (10%) Frame = +2 Query: 149 ISAASETDKARAVPSIILPQGY-IQTHRQTDRQEF-----KPADGAVNLPEPRRTTPSSI 310 +SA + + AR +I+PQ + + R E KP +G+++ P+P PSSI Sbjct: 270 VSAVTASPMARKPQPVIIPQEHSVAVSRSFTNPERRVSPPKPVNGSISPPKPITGKPSSI 329 >At5g61390.1 68418.m07702 exonuclease family protein contains exonuclease domain, Pfam:PF00929 Length = 487 Score = 26.6 bits (56), Expect = 8.4 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 2/60 (3%) Frame = +2 Query: 218 QTHRQTD--RQEFKPADGAVNLPEPRRTTPSSIFNNCIIMISDLSRETSEWRRRILKKNP 391 QTHR D R F+ + + P + N + + + ETS RRR +KK+P Sbjct: 165 QTHRSLDDVRMNFEVLKYCATVLFLESSLPDELIENSV---TTTTPETSSRRRRTIKKSP 221 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,275,754 Number of Sequences: 28952 Number of extensions: 171551 Number of successful extensions: 524 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 514 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 524 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 791932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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