BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_N06 (583 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 288 5e-79 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 171 8e-44 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 165 4e-42 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 2e-32 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 29 0.50 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 28 0.87 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.5 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 2.0 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.6 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 4.6 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 6.1 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 6.1 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 25 6.1 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.1 SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 8.1 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 25 8.1 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 288 bits (706), Expect = 5e-79 Identities = 131/193 (67%), Positives = 154/193 (79%) Frame = +1 Query: 1 GDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGA 180 G S+ Q ER+NVY+NEA+GGKYVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGA Sbjct: 38 GTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGA 97 Query: 181 GNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIRE 360 GN WAKGHYTEGAEL D+VLDVVR+E+E+CD LQGFQ KIRE Sbjct: 98 GNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIRE 157 Query: 361 EYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICFRTLKL 540 EYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CID EAL I TLK+ Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217 Query: 541 STPTYGDLNHLVS 579 +P+Y DLNHLVS Sbjct: 218 KSPSYDDLNHLVS 230 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 171 bits (415), Expect = 8e-44 Identities = 75/181 (41%), Positives = 114/181 (62%) Frame = +1 Query: 37 YYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEG 216 +++E GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115 Query: 217 AELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSV 396 E++DSVL+ +R+ +++C LQGF ++ EY + +SV Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSV 175 Query: 397 VPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICFRTLKLSTPTYGDLNHLV 576 P+P+VS +VVEPYN+ L+ H ++N+D T+ +D EA YDIC R L + PTY +LN L+ Sbjct: 176 YPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235 Query: 577 S 579 + Sbjct: 236 A 236 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 165 bits (401), Expect = 4e-42 Identities = 73/183 (39%), Positives = 111/183 (60%) Frame = +1 Query: 31 NVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYT 210 + +++E GKYVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Query: 211 EGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTY 390 G ELVD V D +R+ +++C LQGF ++ EY + + Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQF 169 Query: 391 SVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICFRTLKLSTPTYGDLNH 570 SV P+P+VS +VVEPYN+ L+ H ++ D T+ +D E+ YDIC R L + P+Y +LN Sbjct: 170 SVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNR 229 Query: 571 LVS 579 L++ Sbjct: 230 LIA 232 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 133 bits (321), Expect = 2e-32 Identities = 63/190 (33%), Positives = 107/190 (56%), Gaps = 3/190 (1%) Frame = +1 Query: 19 LERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNW 192 ++R +V++ ++ +Y+PRAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNW Sbjct: 45 VDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNW 104 Query: 193 AKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPD 372 A G Y+ + + ++D++ +E++ D L+GF ++ + YP Sbjct: 105 ANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPK 163 Query: 373 RIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDXEALYDICFRTLKLSTP 549 +I+ TYSV P S VSD VV+PYN+ L++ +L N D +D AL I L P Sbjct: 164 KIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223 Query: 550 TYGDLNHLVS 579 T+ N LVS Sbjct: 224 TFHQQNQLVS 233 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 29.1 bits (62), Expect = 0.50 Identities = 29/87 (33%), Positives = 43/87 (49%), Gaps = 7/87 (8%) Frame = +2 Query: 299 TLSE-AVPAPVWAHYLSQRSVKSTPTES*THTQSSP----RPKYQTPSSNPTMRLSQCTN 463 T+S+ AVP+ +A+ +S RSV S T+S T++SP K S T+ S TN Sbjct: 643 TVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSPPFVNNTKPIVAKSPVTVTASSETN 702 Query: 464 --S*KTQTKHTVSXTRHSTIFASARSN 538 S K K + + + +F RSN Sbjct: 703 KKSQKINKKASPRVSLWTKLFGKFRSN 729 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 28.3 bits (60), Expect = 0.87 Identities = 20/77 (25%), Positives = 37/77 (48%) Frame = +2 Query: 353 SVKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSXTRHSTIFASAR 532 S S+P+ S T T +SP + SS+ + S ++S + + + S + S+ +S+ Sbjct: 139 SSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 198 Query: 533 SNYLHPPMEISIT*SPS 583 S+ + IT S S Sbjct: 199 SSSSSSSSSVPITSSTS 215 Score = 25.4 bits (53), Expect = 6.1 Identities = 15/70 (21%), Positives = 34/70 (48%) Frame = +2 Query: 329 WAHYLSQRSVKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSXTRH 508 W+ YL+ V T S + + ++ +PSS+ T + ++S + + + S + Sbjct: 113 WSVYLTGNGVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSS 172 Query: 509 STIFASARSN 538 S+ +S+ S+ Sbjct: 173 SSSSSSSSSS 182 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 1.5 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +1 Query: 193 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 282 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.1 bits (57), Expect = 2.0 Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 1/80 (1%) Frame = +2 Query: 131 SDRYSALTTLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 307 ++ Y +TL P+P+ T+ + S S +LT ++ PN V + N++ S Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101 Query: 308 EAVPAPVWAHYLSQRSVKST 367 A P+ A + +R V +T Sbjct: 102 TAPLNPIDACRVGERKVFTT 121 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 2.6 Identities = 28/117 (23%), Positives = 42/117 (35%), Gaps = 1/117 (0%) Frame = +2 Query: 179 PVTTGPRDTTQRALS*STQFLT*SEKNPNHVIVYKDSNSHTLSEAVPAPVWAHYL-SQRS 355 PVT+ P TT S S + + + N+ I + T L + Sbjct: 485 PVTSTPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTP 544 Query: 356 VKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSXTRHSTIFAS 526 V STP + T S+ P TP ++ +S T T T T S ++ S Sbjct: 545 VTSTPLATTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTS 601 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.6 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 124 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 225 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.8 bits (54), Expect = 4.6 Identities = 14/40 (35%), Positives = 18/40 (45%) Frame = +2 Query: 260 PNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSVKSTPTES 379 P VIV +DS S L+ +P P H L P+ S Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQLQSFVPPQPPSSS 62 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 6.1 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +1 Query: 145 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 276 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 6.1 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -3 Query: 182 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 87 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 25.4 bits (53), Expect = 6.1 Identities = 27/79 (34%), Positives = 35/79 (44%), Gaps = 3/79 (3%) Frame = +2 Query: 287 SNSHTLSEAVPAPVWAHYLSQRSVKSTPTES*THTQSSPRPKYQTPSSNPTMR--LSQCT 460 SNS S VP+ V H S KS T T + SS + + T S+ TM +S T Sbjct: 3672 SNSKVTSNKVPSTVSPHSSSISDTKSPATV--TISSSSGQFTHSTFSTGSTMHNTVSHAT 3729 Query: 461 N-S*KTQTKHTVSXTRHST 514 + S T H + T ST Sbjct: 3730 STSSSFTTSHLPTNTLAST 3748 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.0 bits (52), Expect = 8.1 Identities = 31/102 (30%), Positives = 41/102 (40%), Gaps = 5/102 (4%) Frame = +2 Query: 293 SHTLSEAVPAPVWAH-YLSQRSVKSTPTES*THTQ---SSPRPKYQTPSSNPTMR-LSQC 457 S T SE P+ + L SV S S T + SS P ++ T LS Sbjct: 122 SPTSSEYTPSSTESSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTSSTFSSLSSS 181 Query: 458 TNS*KTQTKHTVSXTRHSTIFASARSNYLHPPMEISIT*SPS 583 T+S + T S T S S S+YL +S + SPS Sbjct: 182 TSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPS 223 >SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 530 Score = 25.0 bits (52), Expect = 8.1 Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 1/80 (1%) Frame = +2 Query: 314 VPAPVWAHYLSQRSVKST-PTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHT 490 V APVW++ S T T S S P ++ SS P ++ + T+ T Sbjct: 121 VTAPVWSNTSVPVSTPETSATSSSEFFTSYPATSSESSSSYPASSTEVASSYSASSTEVT 180 Query: 491 VSXTRHSTIFASARSNYLHP 550 S S + A++ S+Y+ P Sbjct: 181 SSYPASSEV-ATSTSSYVAP 199 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 25.0 bits (52), Expect = 8.1 Identities = 13/52 (25%), Positives = 29/52 (55%) Frame = +2 Query: 383 THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSXTRHSTIFASARSN 538 T + S+P + SS+P+ S+ ++S K+ + + S S+ +S++S+ Sbjct: 148 TSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSSSSSSSKSSSSSSSSSKSS 199 Score = 25.0 bits (52), Expect = 8.1 Identities = 17/62 (27%), Positives = 33/62 (53%) Frame = +2 Query: 353 SVKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSXTRHSTIFASAR 532 S STP+ S + + SSP + SS+ + S ++S K+ + + S S+ +S++ Sbjct: 149 SSSSTPSSSSSSSSSSPSSS-SSKSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSSSK 207 Query: 533 SN 538 S+ Sbjct: 208 SS 209 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.136 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,202,168 Number of Sequences: 5004 Number of extensions: 43300 Number of successful extensions: 168 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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