Samia BLAST Result against mosquito

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_N04
         (535 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U50475-1|AAA93477.1|  207|Anopheles gambiae protein ( Anopheles ...    86   6e-19
U51225-1|AAA96405.1|  692|Anopheles gambiae hexamerin protein.         86   8e-19
AF020870-1|AAC31873.1|  692|Anopheles gambiae hexamerin A protein.     86   8e-19
AF020872-1|AAC31875.1|  692|Anopheles gambiae hexamerin A protein.     85   1e-18
AF020871-1|AAC31874.1|  692|Anopheles gambiae hexamerin A protein.     85   1e-18
AF004916-1|AAB94672.1|  686|Anopheles gambiae pro-phenol oxidase...    48   2e-07
AJ459962-1|CAD31061.1|  685|Anopheles gambiae prophenoloxidase 9...    48   3e-07
AJ010195-1|CAA09034.1|  687|Anopheles gambiae prophenoloxidase p...    47   5e-07
L76038-1|AAC27383.1|  683|Anopheles gambiae prophenoloxidase pro...    41   2e-05
AF031626-1|AAD01936.1|  683|Anopheles gambiae prophenoloxidase p...    41   2e-05
AF004915-1|AAB94671.1|  688|Anopheles gambiae pro-phenol oxidase...    41   3e-05
AJ459960-1|CAD31059.1|  696|Anopheles gambiae prophenoloxidase 7...    37   4e-04
AJ010194-1|CAA09033.1|  684|Anopheles gambiae prophenoloxidase p...    34   0.003
AJ010193-1|CAA09032.1|  684|Anopheles gambiae prophenoloxidase p...    29   0.13 
AJ459961-1|CAD31060.1|  700|Anopheles gambiae prophenoloxidase 8...    27   0.52 
EF588468-1|ABQ96704.1|  176|Anopheles gambiae transposase protein.     24   2.8  
AB090822-2|BAC57920.1| 1173|Anopheles gambiae reverse transcript...    24   3.7  
AF291654-1|AAG00600.1| 1340|Anopheles gambiae thioester-containi...    23   6.4  
DQ370043-1|ABD18604.1|  161|Anopheles gambiae putative TIL domai...    23   8.5  

>U50475-1|AAA93477.1|  207|Anopheles gambiae protein ( Anopheles
           gambiae putativearylphorin precursor, mRNA, partial cds.
           ).
          Length = 207

 Score = 86.2 bits (204), Expect = 6e-19
 Identities = 51/146 (34%), Positives = 78/146 (53%), Gaps = 1/146 (0%)
 Frame = +2

Query: 74  RLRQSLQKGKF-ESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLSLA 250
           ++R+ ++ G F +  G +I+    +++ F GN    N D V R  +   Y     +L L+
Sbjct: 4   KIRKIIEDGFFVKEDGTRINLRLPESVEFFGNLLNSNVDSVDRNYV--GYIEVFSRLLLS 61

Query: 251 MCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHF 430
                    F+ +   PSAL  ++T+LRDP FYQLY R +     FK +L  Y  E+L+F
Sbjct: 62  ------GNDFNAYKVWPSALMQFETSLRDPVFYQLYERFMDLYYYFKRFLPSYTYEELNF 115

Query: 431 VGVKINDVVVEKLVTFFDYXQFDATN 508
            GV I DV  +KL+T+FDY   D +N
Sbjct: 116 NGVVIKDVTFDKLMTYFDYFDSDVSN 141


>U51225-1|AAA96405.1|  692|Anopheles gambiae hexamerin protein.
          Length = 692

 Score = 85.8 bits (203), Expect = 8e-19
 Identities = 56/171 (32%), Positives = 86/171 (50%), Gaps = 5/171 (2%)
 Frame = +2

Query: 11  KAGHYIYTA*ELQAYVLDILK----RLRQSLQKGKF-ESYGKKIDFHDEQAINFVGNYWQ 175
           K+  Y Y   +   Y LD +     ++R+ ++ G F +  G +I+    +++ F GN   
Sbjct: 311 KSRDYNYMISDESYYKLDWINAWEAKIRKIIEDGFFVKEDGTRINLRLPESVEFFGNLLN 370

Query: 176 ENADXV*REKLQQDYSTNLMKLSLAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQL 355
            N D V       +Y   +   S  + S      F+ +   PSAL  ++T+LRDP FYQL
Sbjct: 371 SNVDSV-----DANYVGYIEVFSRLLLS---GNDFNAYKVWPSALMQFETSLRDPVFYQL 422

Query: 356 YXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEKLVTFFDYXQFDATN 508
           Y R +     FK +L  Y  E+L+F GV I DV  +KL+T+FDY   D +N
Sbjct: 423 YERFMDLYYYFKRFLPSYTYEELNFNGVVIKDVTFDKLMTYFDYFDSDVSN 473


>AF020870-1|AAC31873.1|  692|Anopheles gambiae hexamerin A protein.
          Length = 692

 Score = 85.8 bits (203), Expect = 8e-19
 Identities = 56/171 (32%), Positives = 86/171 (50%), Gaps = 5/171 (2%)
 Frame = +2

Query: 11  KAGHYIYTA*ELQAYVLDILK----RLRQSLQKGKF-ESYGKKIDFHDEQAINFVGNYWQ 175
           K+  Y Y   +   Y LD +     ++R+ ++ G F +  G +I+    +++ F GN   
Sbjct: 311 KSRDYNYMISDESYYKLDWINAWEAKIRKIIEDGFFVKEDGTRINLRLPESVEFFGNLLN 370

Query: 176 ENADXV*REKLQQDYSTNLMKLSLAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQL 355
            N D V       +Y   +   S  + S      F+ +   PSAL  ++T+LRDP FYQL
Sbjct: 371 SNVDSV-----DANYVGYIEVFSRLLLS---GNDFNAYKVWPSALMQFETSLRDPVFYQL 422

Query: 356 YXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEKLVTFFDYXQFDATN 508
           Y R +     FK +L  Y  E+L+F GV I DV  +KL+T+FDY   D +N
Sbjct: 423 YERFMDLYYYFKRFLPSYTYEELNFNGVVIKDVTFDKLMTYFDYFDSDVSN 473



 Score = 22.6 bits (46), Expect = 8.5
 Identities = 11/32 (34%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = +2

Query: 284 KHTFMPSALDFYQTALRDPAFYQLYXRI-VGY 376
           K+TF+ ++ DFY +      +  LY +I +GY
Sbjct: 551 KNTFVRNSRDFYWSVKDRTMYTDLYKKIMLGY 582


>AF020872-1|AAC31875.1|  692|Anopheles gambiae hexamerin A protein.
          Length = 692

 Score = 85.0 bits (201), Expect = 1e-18
 Identities = 50/146 (34%), Positives = 77/146 (52%), Gaps = 1/146 (0%)
 Frame = +2

Query: 74  RLRQSLQKGKF-ESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLSLA 250
           ++R+ ++ G F +  G +I+    +++ F GN    N D V       +Y   +   S  
Sbjct: 336 KIRKIIEDGFFVKEDGTRINLRLPESVEFFGNLLNSNVDSV-----DANYVGYIEVFSRL 390

Query: 251 MCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHF 430
           + S      F+ +   PSAL  ++T+LRDP FYQLY R +     FK +L  Y  E+L+F
Sbjct: 391 LLS---GNDFNAYKVWPSALMQFETSLRDPVFYQLYERFMDLYYYFKRFLPSYTYEELNF 447

Query: 431 VGVKINDVVVEKLVTFFDYXQFDATN 508
            GV I DV  +KL+T+FDY   D +N
Sbjct: 448 NGVVIKDVTFDKLMTYFDYFDSDVSN 473


>AF020871-1|AAC31874.1|  692|Anopheles gambiae hexamerin A protein.
          Length = 692

 Score = 85.0 bits (201), Expect = 1e-18
 Identities = 50/146 (34%), Positives = 77/146 (52%), Gaps = 1/146 (0%)
 Frame = +2

Query: 74  RLRQSLQKGKF-ESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLSLA 250
           ++R+ ++ G F +  G +I+    +++ F GN    N D V       +Y   +   S  
Sbjct: 336 KIRKIIEDGFFVKEDGTRINLRLPESVEFFGNLLNSNVDSV-----DANYVGYIEVFSRL 390

Query: 251 MCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHF 430
           + S      F+ +   PSAL  ++T+LRDP FYQLY R +     FK +L  Y  E+L+F
Sbjct: 391 LLS---GNDFNAYKVWPSALMQFETSLRDPVFYQLYERFMDLYYYFKRFLPSYTYEELNF 447

Query: 431 VGVKINDVVVEKLVTFFDYXQFDATN 508
            GV I DV  +KL+T+FDY   D +N
Sbjct: 448 NGVVIKDVTFDKLMTYFDYFDSDVSN 473


>AF004916-1|AAB94672.1|  686|Anopheles gambiae pro-phenol oxidase
           subunit 2 protein.
          Length = 686

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 29/116 (25%), Positives = 59/116 (50%)
 Frame = +2

Query: 116 GKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLSLAMCSVQHPKPFDKHTF 295
           G +I   +   I+ +GN  + +A  V   +   +Y  NL  +     +  H  P ++   
Sbjct: 330 GNRIPLDERTGIDVLGNIMEPSALSV-NSQFYGNYHGNLHNI----IAYSHD-PDNRFLE 383

Query: 296 MPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVE 463
               +  +QTA+RDP+FY+L+ ++    + +K  L+PY   +L++ G++I  + V+
Sbjct: 384 GYGVVGEFQTAMRDPSFYRLHAQVDNMFHRYKRTLQPYNANQLNYNGIQIQSLGVQ 439


>AJ459962-1|CAD31061.1|  685|Anopheles gambiae prophenoloxidase 9
           protein.
          Length = 685

 Score = 47.6 bits (108), Expect = 3e-07
 Identities = 32/133 (24%), Positives = 59/133 (44%), Gaps = 1/133 (0%)
 Frame = +2

Query: 68  LKRLRQSLQKG-KFESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLS 244
           L R+++++Q G    + G ++    ++ I+ +GN  + +   V        Y  N   L 
Sbjct: 314 LGRIKEAIQSGFAMAADGTRVPLDPKKGIDILGNIMENSILSV-----NVPYYGNYHSLG 368

Query: 245 LAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKL 424
             +    H  P + +      +  + TA+RDP FY+ +  +    +  K  L PY   +L
Sbjct: 369 HVLIGFIHD-PDNLYLEGHGVMGDFTTAMRDPTFYRFHGHVDDVFDMHKQKLSPYKAHEL 427

Query: 425 HFVGVKINDVVVE 463
            F GV I+D  V+
Sbjct: 428 SFPGVSISDATVQ 440


>AJ010195-1|CAA09034.1|  687|Anopheles gambiae prophenoloxidase
           protein.
          Length = 687

 Score = 46.8 bits (106), Expect = 5e-07
 Identities = 30/116 (25%), Positives = 58/116 (50%)
 Frame = +2

Query: 116 GKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLSLAMCSVQHPKPFDKHTF 295
           G +I   ++  I+ +GN  + +A  V   +   +Y  ++  L     S  H  P ++   
Sbjct: 331 GNRIPLDEQTGIDVLGNIIEPSALSV-NSQYYGNYHGHMHNL----ISFSHD-PENRFLE 384

Query: 296 MPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVE 463
               +  +QTA+RDPAFY+L+ ++    + +K  L+PY   ++ + GV+I    V+
Sbjct: 385 GYGVVGEFQTAMRDPAFYRLHAQVDNMFHRYKRTLQPYNANQIGYAGVQIQSFGVQ 440


>L76038-1|AAC27383.1|  683|Anopheles gambiae prophenoloxidase
           protein.
          Length = 683

 Score = 41.1 bits (92), Expect = 2e-05
 Identities = 30/133 (22%), Positives = 61/133 (45%), Gaps = 1/133 (0%)
 Frame = +2

Query: 68  LKRLRQSLQKG-KFESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLS 244
           + R+ +++ +G   +  G +I   +++ I+ +GN  + +     R+     ++   + +S
Sbjct: 313 IDRIYEAIHQGFVVDESGNRIPLDEQKGIDHLGNIIESSILSPNRQLYGDMHNMGHVFIS 372

Query: 245 LAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKL 424
            A     H  P  +H      +    TA+RDP FY+ +  I       K+ L PY + +L
Sbjct: 373 YA-----HD-PDHRHLESFGVMGDVATAMRDPVFYRWHSYIDDIFQEHKNKLPPYTRSQL 426

Query: 425 HFVGVKINDVVVE 463
            F G+ I  + V+
Sbjct: 427 TFDGISITGITVQ 439


>AF031626-1|AAD01936.1|  683|Anopheles gambiae prophenoloxidase
           protein.
          Length = 683

 Score = 41.1 bits (92), Expect = 2e-05
 Identities = 30/133 (22%), Positives = 61/133 (45%), Gaps = 1/133 (0%)
 Frame = +2

Query: 68  LKRLRQSLQKG-KFESYGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLS 244
           + R+ +++ +G   +  G +I   +++ I+ +GN  + +     R+     ++   + +S
Sbjct: 313 IDRIYEAIHQGFVVDESGNRIPLDEQKGIDHLGNIIESSILSPNRQLYGDMHNMGHVFIS 372

Query: 245 LAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKL 424
            A     H  P  +H      +    TA+RDP FY+ +  I       K+ L PY + +L
Sbjct: 373 YA-----HD-PDHRHLESFGVMGDVATAMRDPVFYRWHSYIDDIFQEHKNKLPPYTRSQL 426

Query: 425 HFVGVKINDVVVE 463
            F G+ I  + V+
Sbjct: 427 TFDGISITGITVQ 439


>AF004915-1|AAB94671.1|  688|Anopheles gambiae pro-phenol oxidase
           subunit 1 protein.
          Length = 688

 Score = 40.7 bits (91), Expect = 3e-05
 Identities = 20/47 (42%), Positives = 26/47 (55%)
 Frame = +2

Query: 323 TALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVE 463
           TA+RDP FY+ +  I G     K  L PY  E+L   GV +N V V+
Sbjct: 393 TAMRDPIFYRWHGMIDGIFRRHKELLTPYTAEQLGNPGVTVNSVGVQ 439


>AJ459960-1|CAD31059.1|  696|Anopheles gambiae prophenoloxidase 7
           protein.
          Length = 696

 Score = 37.1 bits (82), Expect = 4e-04
 Identities = 36/154 (23%), Positives = 67/154 (43%), Gaps = 10/154 (6%)
 Frame = +2

Query: 68  LKRLRQSLQKGKFES-YGKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLS 244
           L+R+  ++ +G   +  G++    + + I+ +GN  + ++  V R+     +++  + LS
Sbjct: 327 LQRIIAAIDEGVVVAPSGQRTQLDNFRGIDILGNIVESSSISVNRQLYGDTHNSGHVLLS 386

Query: 245 LAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKL 424
           L    V  P+     +F    +    TA+RDP FY+ +  +       K    PY   +L
Sbjct: 387 L----VHDPREDFLESF--GVMGDVTTAMRDPVFYRWHTFVDSIFQRHKQRFAPYGPAEL 440

Query: 425 HFVGVKI---------NDVVVEKLVTFFDYXQFD 499
              GV +          D V   L+TF+   QFD
Sbjct: 441 RNPGVNLLSLETELDRRDSVKNTLLTFWQRSQFD 474



 Score = 25.4 bits (53), Expect = 1.2
 Identities = 8/40 (20%), Positives = 22/40 (55%)
 Frame = +2

Query: 347 YQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEK 466
           + L  R+   + +F+ ++ PY   ++  + ++  + VVE+
Sbjct: 656 FPLDRRVANTVRSFQDFVAPYQNMRVATITIRFTNTVVER 695


>AJ010194-1|CAA09033.1|  684|Anopheles gambiae prophenoloxidase
           protein.
          Length = 684

 Score = 33.9 bits (74), Expect = 0.003
 Identities = 34/154 (22%), Positives = 65/154 (42%), Gaps = 10/154 (6%)
 Frame = +2

Query: 68  LKRLRQSLQKGKFESY-GKKIDFHDEQAINFVGNYWQENADXV*REKLQQDYSTNLMKLS 244
           +KR+ +++  G  ++  G ++   +E+ I+ +G+  + + + +       DY  N     
Sbjct: 314 IKRIFEAIDNGYAQATNGDRVPLDNEKGIDLIGDLLEASTNSI-NFNYYGDYHQN----G 368

Query: 245 LAMCSVQHPKPFDKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKL 424
             M    H  P + +      +    T +RDP FY+ +  I       K  L  Y   +L
Sbjct: 369 HVMLGYIHD-PDNSYLEGVGVMGDLTTTMRDPLFYRWHQHIDDIFVRHKQRLPAYTSSEL 427

Query: 425 HFVGVKIN--DVVVEK-------LVTFFDYXQFD 499
            F  + ++  DV + K       L+TF+   QFD
Sbjct: 428 SFNDITVDSFDVQLNKANAPKNVLLTFWQRSQFD 461


>AJ010193-1|CAA09032.1|  684|Anopheles gambiae prophenoloxidase
           protein.
          Length = 684

 Score = 28.7 bits (61), Expect = 0.13
 Identities = 14/47 (29%), Positives = 23/47 (48%)
 Frame = +2

Query: 323 TALRDPAFYQLYXRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVE 463
           TA+RDP FY+ +  I       K  L  Y  ++L F  V ++   ++
Sbjct: 394 TAMRDPVFYRWHQHIDDIFVRHKQRLPAYTGQELAFNDVAVDSFEIQ 440


>AJ459961-1|CAD31060.1|  700|Anopheles gambiae prophenoloxidase 8
           protein.
          Length = 700

 Score = 26.6 bits (56), Expect = 0.52
 Identities = 34/137 (24%), Positives = 62/137 (45%), Gaps = 6/137 (4%)
 Frame = +2

Query: 32  TA*ELQAYVLDI---LKRLRQSLQKG-KFESYGKKIDFHDEQAINFVGNYWQENADXV*R 199
           +A  L+  + DI   L R+ +++  G      G ++   + + I+ +GN  + +A  + R
Sbjct: 313 SADRLEVRIADIESWLPRVLEAIDAGFAVSDDGVRVPLDETRGIDVLGNILERSAISINR 372

Query: 200 EKLQQDYSTNLMKLSLAMCSVQHPKPFDKHTFMPSA--LDFYQTALRDPAFYQLYXRIVG 373
             L  D   N+  + LA   +  P+     T++ S+  +    TA+RDP FY+ +  I  
Sbjct: 373 N-LYGDVH-NMGHVLLAF--IHDPRG----TYLESSGVMGGVATAMRDPIFYRWHKFIDN 424

Query: 374 YINAFKHYLKPYPQEKL 424
                K  L PY   +L
Sbjct: 425 IFLRNKARLAPYTMAEL 441


>EF588468-1|ABQ96704.1|  176|Anopheles gambiae transposase protein.
          Length = 176

 Score = 24.2 bits (50), Expect = 2.8
 Identities = 9/28 (32%), Positives = 17/28 (60%)
 Frame = +3

Query: 33  QRKNYKHTFWTF*KDFVSPYRKANSNRM 116
           + +N+K  F+T   +++ P RK  SN +
Sbjct: 126 ESENFKKFFYTLNPNYIMPTRKCLSNAL 153


>AB090822-2|BAC57920.1| 1173|Anopheles gambiae reverse transcriptase
           protein.
          Length = 1173

 Score = 23.8 bits (49), Expect = 3.7
 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
 Frame = +1

Query: 175 RERRXCMKREVTTG---LFNESYEIVARHVLGA 264
           +ERR  +KR +TTG   LF +  + V  +V G+
Sbjct: 346 QERRSELKRAITTGKGQLFQQQIDEVNANVYGS 378


>AF291654-1|AAG00600.1| 1340|Anopheles gambiae thioester-containing
            protein I protein.
          Length = 1340

 Score = 23.0 bits (47), Expect = 6.4
 Identities = 11/39 (28%), Positives = 21/39 (53%)
 Frame = +2

Query: 281  DKHTFMPSALDFYQTALRDPAFYQLYXRIVGYINAFKHY 397
            +++ F  +A   ++ AL+ PA+  +Y      +NA K Y
Sbjct: 1280 ERNCFTVTAYRRFKVALKRPAYVVVYDYYNTNLNAIKVY 1318


>DQ370043-1|ABD18604.1|  161|Anopheles gambiae putative TIL domain
           polypeptide protein.
          Length = 161

 Score = 22.6 bits (46), Expect = 8.5
 Identities = 7/15 (46%), Positives = 10/15 (66%)
 Frame = +3

Query: 240 CRSPCARCSTLNHST 284
           CR  C+RC T  H++
Sbjct: 120 CRDECSRCVTTIHTS 134


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 535,981
Number of Sequences: 2352
Number of extensions: 11184
Number of successful extensions: 33
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33
length of database: 563,979
effective HSP length: 60
effective length of database: 422,859
effective search space used: 49474503
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)